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The Antibacterial Activity Test Comparison of Green and Black Tea Ethanol Extract (Camellia sinensis) Against Propionibacterium acnes Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Science Midwifery Vol 10 No 2 (2022): April: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i2.544

Abstract

A study was conducted in connection with the comparison of antibacterial activity tests between ethanol extracts from green tea leaves and black tea leaves (Camellia sinensis) against Propionibacterium acnes. There were obtained by a softening technique that was softened for 24 hours. The filtrate was then concentrated to give a concentrated extract and evaporated ethanol content. The concentrated extract was then diluted with distilled water to extract 5%, 10%, and 15% concentration variations. The test method for inhibitory activity was the disc method using distilled water as a negative blank. The results obtained with blank, 5%, 10% and 15% green tea were 0. 5.25; 7.05 and 7.95 mm and black tea ethanol extract were 0; 2.50; 3.20 and 5.30 mm. The conclusion of this study is that the concentration of the extract is directly proportional to its inhibitory power, and the green tea ethanol extract is more potent than the black tea ethanol extract, so both can be used as antibacterial agents against P. acnes.
The Inhibitory Activity Test of Green Tea Ethanol Extract (Camellia sinensis) Sidamanik Against Escherichia coli Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Khaidir
Science Midwifery Vol 10 No 2 (2022): April: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A study was done in analyzing the inhibitory activity of green tea ethanol extracts against Escherichia coli. The green tea is sourced from a tea plantation in Sidamanik, North Sumatra. The green tea was macerated with ethanol 96 % for 24 hours. After that, the filtrate was taken and then concentrated to give a concentrated extract and evaporated ethanol content until run out and obtained thick extract. The thick extract was then diluted with distilled water to extract 5%, 10%, and 15% concentration variations. The test method for inhibitory activity was the disc method using distilled water as a negative blank. The results obtained with blank, 5%, 10% and 15% green tea were 0. 5.7; 7.10, and 8.05 mm The conclusion of this study is that the concentration of the extract is directly proportional to its inhibitory power, and can be used as an antibacterial agent against E. coli.
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 9, No 1 (2022): August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
Isolasi dan Karakterisasi Bakteri Asam Laktat dari Teh Hijau Fermentasi Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 3, No 2 (2022): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v3i2.9645

Abstract

ABSTRAKTeh hijau adalah jenis teh yang tidak mengalami proses fermentasi akan tetapi mengalami proses pengeringan dan penguapan daun. Teh hijau memiliki aktivitas antioksidan yang tinggi karena kandungan katekin di dalamnya. Namun bioavaibilitas katekin teh hijau berkurang pada saluran cerna karena kurang stabil. Kehadiran probiotik pada teh hijau diharapkan mampu meningkatkan bioavaibilitasnya. Bakteri Asam Laktat (BAL) merupakan kelompok bakteri baik yang merupakan probiotik yang berperan dalam menyehatkan saluran cerna. Peneltian ini bertujuan untuk mengisolasi dan menguji karakterisasi BAL dari Teh Hijau Fermentasi. Teh hijau yang berasal dari Distributor Teh Juma, Kecamatan Sidamanik difermentasi dengan gula aren dan madu hutan dengan variasi waktu 24, 48 dan 72 jam pada suhu rendah. Teh hijau fermentasi kemudian diisolasi kemudian diencerkan dengan pengenceran 10-5 dan 10-6. Hasil inkubasi selama 48 jam diperoleh kemudian dimurnikan dengan metode streak dan diinkubasi kembali selama 24 jam kemudian diuji karakterisasinya secara morfologi, pewarnaan gram, uji katalase dan uji fermentasi. Hasil yang diperoleh adalah pada uji morfologi positif BAL dengan ciri-ciri terlihat koloni tunggal dengan bentuk bulat, licin, warna putih kekuningan.dan terbentuk zona bening di sekitar koloni. Hasil pewarnaan positif BAL dengan ciri-ciri bakteri gram positif ditunjukkkan oleh warna biru ke ungu. Hasil uji katalase ditunjukkan dengan ciri-ciri  tidak menghasilkan gelembung udara yang menandakan adanya BAL karena BAL tidak mengandung oksigen serta uji fermentasi yang ditunjukkan dengan tidak terbentuknya gelembung udara pada tabung Durham yang menandakan tipe fermentasi BAL adalah homofermentatif. Kata kunci : Isolasi; Karakterisasi; Bakteri asam laktat; Teh hijau; Fermentasi.ABSTRACTGreen tea is a type of tea that does not undergo a fermentation process but undergoes a process of drying and evaporation of the leaves. Green tea has high antioxidant activity due to the catechin content in it. However, the bioavailability of green tea catechins is reduced in the gastrointestinal tract because it is less stable. The presence of probiotics in green tea is expected to increase its bioavailability. Lactic Acid Bacteria (LAB) is a group of good bacteria which are probiotics that play a role in healthy gastrointestinal tract. This study aims to isolate and test the characterization of LAB from Fermented Green Tea. Green tea from Juma Tea Distributor, Sidamanik District was fermented with palm sugar and forest honey with variations of 24, 48 and 72 hours at low temperature. The fermented green tea was isolated and then diluted with 10-5 and 10-6 dilutions. The results of incubation for 48 hours were obtained and then purified by streak method and incubated again for 24 hours and then tested for morphological characterization, gram staining, catalase test and fermentation test. The results obtained were a positive morphological test of LAB with the characteristics of a single colony being seen with a round, smooth, yellowish white color, and a clear zone was formed around the colony. The results of positive LAB staining with gram-positive bacteria are indicated by a blue to purple color. The results of the catalase test are indicated by the characteristics of not producing air bubbles which indicate the presence of LAB because LAB does not contain oxygen and the fermentation test is indicated by the absence of air bubbles in the Durham tube which indicates the type of LAB fermentation is homofermentative. Keywords : Isolation; Characterization; Lactic acid bacteria; Green tea; Fermentation;
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 1 (2022): August 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
PHYTOCHEMICAL SCREENING OF THREE BATAK LEEKS VARIANS (A. chinense G. Don) Aliyah Fahmi
JURNAL KIMIA MULAWARMAN Vol 19 No 1 (2021)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v19i1.1002

Abstract

"Phytochemical screening of Three Batak Leeks (A. chinense G. Don) Varians " has been carried out. For phytochemical screening, secondary metabolites were analyzed, including alkaloids, phenolics, flavonoids, terpenoids, steroids and saponins. From the three samples of Batak Leeks (fresh, dried and methanol extract), the result was concluded that secondary metabolites of the terpenoids were weak in the methanol extract and not detected in the fresh and dried Batak leeks .
Applications and Implications from Epigallo Catechin Gallate (EGCG) in Green Tea Fahmi, Aliyah; Syukur, Sumaryati; Chaidir, Zulkarnain; Melia, Sri
Journal of Midwifery and Nursing Vol. 6 No. 1 (2024): January: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v6i1.4287

Abstract

Epigallo catechin gallate (EGCG), a catechin bioactive component found in green tea, has been used widely as a popular beverage in Asian nations for centuries. According to a summary of five years’ worth of recent papers, EGCG has a wide range of positive benefits including anticancer, anti-cholesterol, cardiovascular agent, anti-virus, antioxidant, and adjuvant treatment agent. The purpose of this literature review is to get more knowledge on EGCG, the most prevalent catechin derivative found in green tea. A substance with significant antioxidant capabilities, EGCG has a wide range of applications. However, EGCG also has a number of drawbacks in addition to its benefits, such as low stability, poor absorption, and the requirement for a daily intake restriction because it acts as a pro-oxidant and can be harmful if consumed in excess. As a result, authors attempt to mitigate undesirable effects and discover a way to maximize EGCG benefits through additional study that serves as research material. Furthermore, we are becoming research subjects in order to against the negative effects of EGCG, such as how to use probiotics to boost the absorption of EGCG during digestion.
Production Process of Large Pore Size Activated Carbon from Palm Kernel Shell using Sodium Chloride as An Activator Nurdin, Ahmad; Iriani, Iriani; Harahap, Hamidah; Fahmi, Aliyah
Indonesian Journal of Chemical Research Vol 10 No 1 (2022): Edition for May 2022
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2022.10-ahm

Abstract

This study aimed to determine the yield of activated carbon, iodine number, and surface area of palm activated carbon. Samples were produced by using sodium chloride (NaCl) as an activator. Palm shells that had been produced by the milling process were then sieved with a 12 mesh sieve and soaked in 20 % NaCl solution. The sample solution was heated over a water bath at 70 oC and continued with the drying process at a constant temperature of 105 oC. The activated shells continued the pyrolysis process at temperatures of 300, 400, and 500 oC for 3 hours. The activated carbon obtained from the pyrolysis process was weighed and then washed using hot distilled water. The samples were dried in an oven at a temperature of 105 oC for 24 hours. The results were analyzed for iodine number using iodometric titration method, surface area using Brunauer- Emmett-Teller (BET) method, and pore structure using the Scanning Electron Microscope (SEM) method. The results showed the best yield was 38.13 % obtained at 20% NaCl and a temperature of 400 oC. The best iodine number was 767.745 mg/g and surface area was 6.790 m2/g, pore volume 4.377 cc/g with pore size 9.781 A.
PENYULUHAN SERTA PEMBAGIAN MASKER DI MASA PANDEMI COVID-19 KEPADA PARA PEDAGANG PASAR DI DESA DURIN TONGGAL SUMATERA UTARA Fahmi, Aliyah; Sitompul, Hamela
Reswara: Jurnal Pengabdian Kepada Masyarakat Vol 3, No 1 (2022)
Publisher : Universitas Dharmawangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46576/rjpkm.v3i1.922

Abstract

Kegiatan pengabdian masyarakat di tengah pandemi corona virus disease-19 (COVID-19) sepatutnya di lakukan secara luas, mengingat masih banyak masyarakat yang kurang mematuhi protokol kesehatan dalam rangka mencegah penyebaran COVID-19. Pemerintah Indonesia telah menetapkan langkah-langkah dan upaya pencegahan pandemi COVID-19, salah satunya adalah dengan mensosialisasikan gerakan memakai masker terutama jika berada di tempat umum. Salah satu tempat umum yang berpotensi dalam penyebaran COVID 19 ini adalah pasar dimana di pasar berkumpul masyarakat dari latar belakang yang berbeda-beda dengan kondisi kesehatan yang kita belum ketahui secara pasti. Pasar merupakan tempat untuk melakukan transaksi jual beli dalam memenuhi kebutuhan hidupnya baik kebutuhan primer, sekunder dan tersier. Untuk itu perlu dilakukan sosialisasi untuk pencegahan dengan menggunakan masker bagi pedagang maupun pembeli yang melakukan transaksi jual beli tersebut. Tujuan dari pengabdian ini adalah untuk mencegah dan memutus mata rantai penularan COVID-19 di Pasar Desa Durin Tonggal. Pengabdian pada Masyarakat dilaksanakan dalam bentuk penyuluhan penggunaan masker sekaligus membagikan masker secara gratis kepada pedagang pasar. Metode yang digunakan dalam pelaksanaan pengabdian ini adalah edukasi kepada pedagang pasar dengan menghimbau pentingnya menggunakan masker. Setelah diberikan edukasi dan pembagian masker, pedagang sudah mulai sadar pentingnya penggunaan masker di Pasar