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All Journal HAYATI Journal of Biosciences Makara Journal of Science Jurnal Kedokteran Hewan Indonesian Journal of Biomedicine and Clinical Sciences
Purba, Hastuti Handayani S
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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Modulation of the NF-κB Activation Pathway by Phycocyanobilin from Spirulina platensis: An in Silico Study Iswanti, Febriana Catur; Purba, Hastuti Handayani S; Prijanti, Ani Retno; Fadilah, Fadilah; Herlina, Linda; Paramita, Reni
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

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Abstract

Several studies have predicted the molecular interactions of the active ingredient of Spirulina platensis as an anti-inflammatory compound. However, these interaction studies did not review the modulation of the NF-κB activation pathway, which involves various factors. This study demonstrated the potential of the bioactive compounds of S. platensis for modulating immune function by reducing inflammation through the inhibition of the NF-κB activation pathway. Phycocyanobilin was predicted to have good potential for molecular docking with multisubunit IκB kinase (IKK)1/IKKA, IKK2/IKKB, NF-κB-inducing kinase, and the IκBα/NF-κB complex. Furthermore, β-carotene exhibited good potential for interactions with NF-κB essential modulator/IKK and the NF-κB complex, and α-glucan had the potential for interactions with COX-2. Therefore, supplementation with S. platensisand its bioactive compounds is expected to provide optimal benefits. Several studies have predicted the molecular interactions of the active ingredient of Spirulina platensis as an anti-inflammatory compound. However, these interaction studies did not review the modulation of the NF-κB activation pathway, which involves various factors. This study demonstrated the potential of the bioactive compounds of S. platensis for modulating immune function by reducing inflammation through the inhibition of the NF-κB activation pathway. Phycocyanobilin was predicted to have good potential for molecular docking with multisubunit IκB kinase (IKK)1/IKKA, IKK2/IKKB, NF-κB-inducing kinase, and the IκBα/NF-κB complex. Furthermore, β-carotene exhibited good potential for interactions with NF-κB essential modulator/IKK and the NF-κB complex, and α-glucan had the potential for interactions with COX-2. Therefore, supplementation with S. platensis and its bioactive compounds is expected to provide optimal benefits.
Supplementation Impact of Spirulina platensis Ethanol Extract on Inflammatory Homeostasis Modulation of Rat Spleen at Different Ages Paramita, Reni; Purba, Hastuti Handayani S; Prijanti, Ani Retno; Iswanti, Febriana Catur
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1231-1242

Abstract

Pro- and anti-inflammatory mediators need to be released in a balanced way to maintain a healthy state as we age. One important regulatory element in the equilibrium of pro- and anti-inflammatory mediators is NF-κB. The purpose of this study was to examine how S. platensis affected the control of inflammatory mediators in young, healthy, emerging adults and adults in rats. In this investigation, 200 mg/kg BW of S. platensis extract was administered to six groups of male Wistar rats, ages 12, 18, and 24 weeks, along with a control group. In both the treatment and control groups, NF-κB p65 protein expression was lower at 24 weeks than it was at 12 and 18 weeks. TNF-α and COX-2 proteins were lower in the treatment group than in the control group. All age groups in the treatment group had higher levels of IL-10 protein than the control group. The quantity of NF-κB p65 was positively correlated with COX-2 and TNF-α. By raising the concentration of NF-κB p65, the ethanolic extract of S. platensis altered a mediator of cellular immunity. A decrease followed this in TNF-α and COX-2 and a rise in IL-10 in the rat spleen at different ages.
Potential biomarkers of IFN-γ, IL-2 and CXCL9 for diagnosis of Q fever disease Purba, Hastuti Handayani S; Yasmon, Andi
Indonesian Journal of Biomedicine and Clinical Sciences Vol 56 No 2 (2024)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v56i2.12991

Abstract

The pathogen responsible for Q fever disease, Coxiella burnetii, is a zoonosis classified as a pathogen due to its airborne transmission. The C. burnetii infection could be both acute or chronic in humans. The main and most common entry of the pathogens to the body is through the breathing of polluted aerosols containing a resistant substance similar to C. burnetii spores. This small cell variant (SCV) or spore-like morphotype is extremely stress-resistant, therefore inadequate treatment causes serious effects even death. Due to the diversity of clinical manifestations of Q fever and the presence of less specific and sensitive diagnoses for other diseases, multiple platforms for exploring Q fever biomarkers are required. Apart from serological studies to determine a biomarker for Q fever, it will be prudent to concentrate on the more appropriate cell-mediated immune response. This article discusses C. burnetii causing Q fever disease and how the host develops humoral and cellular immunity, particularly IFN-γ, IL-2 and CXCL9, as potential biomarkers for the diagnosis of Q fever disease.
SEROLOGICAL DETECTION OF BOVINE LEPTOSPIROSIS IN SUKABUMI AND CIANJUR REGIONS Susanti, Susanti; Rachmawati, Faidah; Mulyadi, Andi; Sukatma, Sukatma; Purba, Hastuti Handayani S; Anastasia, Yessy; Ariyanti, Tati; Prihandani, Sri Suryatmiati
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.33751

Abstract

This study aims to detect leptospirosis serologically and serovars found in cattle in the Sukabumi and Cianjur areas. 165 cattle serum samples were taken randomly in the Sukabumi and Cianjur region. Microscopic Agglutination Tests (MAT) were used for serological testing using the antigens of Leptospira serovar Icterohaemorrhagiae, Javanica, Celledoni, Canicola, Ballum, Pyrogenes, Cynopteri, Rachmati, Australis, Pomona, Grippotyphosa, Hardjo, Bataviae, and Tarassovi. The MAT results showed that positive serum leptospirosis in cattle in Sukabumi region was 18.75%, with serovars Hardjo 16.25%, Tarassovi 1.25%, and Hardjo and Tarassovi (1.25%). Meanwhile, in Cianjur region, positive serum was 30.60% with serovar Hardjo 22.35%, Tarassovi 2.35%, Icterohaemorrhagiae 1.18%, Grippotyphosa 1.18%, Hardjo and Tarassovi 1.18%, Cynopteri and Hardjo (1.18%), Rachmati, Pomona, Bataviae and Tarassovi (1.18%). Leptospira serovar Hardjo was the dominant serovar in both regions. Overall, one serovar was detected in 22.42% of cattle, two serovars in 1.82% and four serovars in 0.61% cattle.
Co-Authors Andi Mulyadi Andi Yasmon Ani Retno Prijanti Fadilah Fadilah, Fadilah Febriana Catur Iswanti Linda Herlina Rachmawati, Faidah Reni Paramita Sri Suryatmiati Prihandani Sukatma Sukatma Susanti Susanti Tati Ariyanti Yessy Anastasia, Yessy