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All Journal Jurnal Kesehatan Masyarakat Indonesia Makara Journal of Science
Hermawati, Ajitya Kurnia
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Public Health

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ISOLASI DAN SKRINING MOLEKULER BAKTERI ASAM LAKTAT PEMBAWA GEN GLUKANSUKRASE DARI MAKANAN DAN MINUMAN MENGANDUNG GULA Malik, Amarila; Hermawati, Ajitya Kurnia; Hestiningtyas, Mahardhika; Radji, Maksum
Makara Journal of Science Vol. 14, No. 1
Publisher : UI Scholars Hub

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Abstract

Isolation and Molecular Screening of Glucansucrase Gene Harboring-Lactic Acid Bacteria. Exopolysaccharides (EPS) have been possessed to be used in pharmaceutical, cosmetic and food industries. Lactic acid bacteria (LAB) produce a wide variety of exopolysaccharides and carries sucrase genes glucansucrase/glucosyltransferase (gtf) and fructansucrase/fructosyltransferases (ftf) for EPS production. In this study, the isolation and screening of EPS producing-LAB (EPS-LAB) were carried out on modified de Mann-Rogosa-Sharpe (MRS) agar medium supplemented with 10% of sucrose on LAB isolated from various unique sugar containing-foods and -beverages originated from local sources. Besides obtaining EPS-LAB, this study aimed to screen for gtf gene as well as to molecular identify strains by using PCR technique. Degenerate primer pairs DegFor and DegRev which targeted the conserved region of gtf genes catalytic domain were used, whereas LABfw and LABrv were used to molecular identify strains using 16S rRNA gene. An approximately 660 base pairs (bp) amplicons which targeted gtf gene were obtained from 13 out of 16 srains chosen. Result from PCR of 16S rRNA gene identification on gtf positive strains revealed that all strains were molecular identified as LAB after DNA sequencing analysis by using blastn. A rare EPS-producing LAB were obtain i.e. Weissella. Strains obtained in this study are potential sources for exploring novel sucrase gene/s and obtain unique EPS polymer product/s.
The Impact of Lean Management Implementation on Inpatient Discharge Prescription Services at Pertamina Central Hospital Hermawati, Ajitya Kurnia; Kristin, Erna; Firman, Firman
Jurnal Kesehatan Masyarakat Indonesia Vol 19, No 3 (2024): Juli - September 2024
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jkmi.19.3.2024.25-31

Abstract

Background: The lead time of inpatient discharge prescription services affects the overall discharge process. In 2022, lead times at Pertamina Central Hospital's Inpatient Pharmacy varied, requiring continuous efforts to meet target times and avoid disrupting discharge plans. This research aims to evaluate the impact of lean management on reducing these lead times. Methods: The study employs a combined quantitative and qualitative research design with an action research approach. It was conducted from March 4 to May 8, 2023, involving pharmacists, pharmaceutical technical personnel, compounders, and pharmacy couriers. Data analysis included measuring lead time changes pre- and post-lean management implementation and narrative analysis of qualitative findings. Results: After implementing lean management, the lead time for non-compounded prescriptions decreased from 42.6 to 25 minutes, with value added ratio (VAR) increasing from 18.9% to 27%. For compounded prescriptions, lead time dropped from 67 to 45.7 minutes, and VAR rose from 25.2% to 37.9%. The Mann-Whitney U test confirmed the significant difference in average lead time. Conclusion: The implementation of lean management in inpatient discharge prescription services has been proven to improve lead time and increase process efficiency (VAR).
Co-Authors Amarila Malik Erna Kristin Firman Firman Hestiningtyas, Mahardhika Maksum Radji