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All Journal HAYATI Journal of Biosciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Biotechnology Sari Pediatri Paediatrica Indonesiana Belitung Nursing Journal Indonesian Journal of Chemistry Biota Jurnal Ilmu Kefarmasian Indonesia Pharmaceutical Sciences and Research (PSR) Makara Journal of Science

Amarila Malik

Faculty Of Pharmacy, Universitas Indonesia

Author-ID : 225628

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Characterization of Trypsin-Like Protease of Lactobacillus plantarum FNCC 0270 Trismilah Margono; Wahono Sumaryono; Amarila Malik; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 21 No. 2 (2014): June 2014
Publisher : Bogor Agricultural University, Indonesia

Trypsin is an enzyme that has a unique mechanism of cutting peptide bonds specifically at the carboxyl side of lysine or arginine amino acids, with another amino acid. This study aims to analyze a trypsin-like protease (TLP) found in Lactobacillus plantarum FNCC 0270, by performing partial proteomic tests, i.e. MALDI-TOF/TOF, and standard bioinformatics tools. SDS-PAGE analysis showed 4 protein bands. Two bands of the (P1 and P2) showed molecular weights equivalent to 47.35 and 38.42 kD, each generating 8 and 11 peptide fragments respectively. According to information in www.ncbi.nlm.nih.gov/genbank/structures, the structure of serine protease HtrA (subs. plantarum L. plantarum ST–III) consists of three domains. Using Clone Manager® software by aligning two sequences we obtained eleven. The Lactobacillus produces of the trypsin-like serine protease has 40-90% similarity. Using the Clustal W2 software we passed the 11 sequences through multiple alignments, and found that the isolate L. plantarum is closely related to L. buchneri, L. brevis, and L. malefermentans on the phylogenetic tree. Alignment analysis results showed that all 8 peptide fragments of band 1 and 11 peptide fragments of band 2, of the SDS-PAGE, were located in the active domain region of the fourth trypsin-like serine protease producing Lactobacilli.

Sucrase Activity and Exopolysaccharide Partial Characterization From Three Weissella confusa Strains Amarila Malik; Sheilla Sheilla; Wangi Firdausi; Tri Handayani; Endang Saepudin
HAYATI Journal of Biosciences Vol. 22 No. 3 (2015): July 2015
Publisher : Bogor Agricultural University, Indonesia

Exopolysaccharides (EPSs) produced by lactic acid bacteria have been well known for their important economic value in food, pharmaceutical and health industries. Large extracellular enzyme sucrases are used by lactic acid bacteria to polymerize EPS, i.e. fructansucrase and glucansucrase. This study aimed to characterize sucrase activity of three Weissella confusa strains MBF8-1, MBF8-2 and MBFCNC-2(1), which were isolated previously from local beverages and their EPS products as well. All strains showed ability to form mucoid and slimy colonies by visual inspection on agar plate using raffinose as substrate suggesting that they possessed fructansucrase activity besides glucansucrase. Obtained EPS products were characterized by HPLC analysis after hydrolysis using 3% TCA at 100C for 1 hour, and by viscosity as well. All strains exhibited similar peak patterns, assuming that all of them possessed fructan EPS product. Supernatant and cell pellet were also analyzed by in situ activity assay performing periodic acid Schiff staining after polyacrylamide gel electrophoresis; only cell pellet showed sucrase activity. Viscosity observation showed that EPS products from all strains were able to increase the viscosity slightly.

Profiling Skin Microbiome in Healthy Young Adult Representing Javanese, Papuans, and Chinese Descent in Indonesia Stella Vania; Amarila Malik
HAYATI Journal of Biosciences Vol. 28 No. 4 (2021): October 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.4.249-261

Skin serves as the first physical barrier and biological barrier by the colonization of commensal bacteria to prevent pathogen invasion. It was known that the disruption on normal commensal microbiota composition or dysbiosis causes skin diseases, while the skin microbiota diversity itself is influenced by several factors, one of them is ethnicity. This study shows the influence of ethnicity factor in Papuans, Javanese, and Chinese descent young adults living in Jakarta on skin microbiome profiles. The microbiota genomic DNA are extracted from the face skin samples and sequenced with Next Generation Sequencing method to be further analyzed. The result shows that individuals with the same ethnic background share similar skin microbiome characteristics. The greatest skin microbiome alpha diversity is shown by the Papuans and the Chinese descent the smallest. Ethnicity factor that shows statistically significant differences in interindividual dissimilarities are independent of other intriguing factors such as age, geographical location, etc. Therefore the ethnic origin of individuals especially from three ethnics above is a factor to be considered in skin microbiome research and the skin microbiota composition can be used for potential future applications.

Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient Hanifah Rahmi; Tiodinar Theresia; Amarila Malik; Septelia Inawati W
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

A therapy development with recombinant protein is a new and potential innovation to destroy cancer stem cells (CSCs). Various ways of killing CSCs include the provision of polyvalent anti-protein antibodies that code pluripotency. Therefore, it takes a mixture of Oct-4, c-Myc, Sox2, and Klf4 protein antigens that can stimulate the formation of polyvalent antibodies. This study aimed to construct Sox2 recombinant by identifying the target genes by reverse transcriptase PCR and then arranging their designs to be inserted into the cloning vector pET101/D-TOPOÂ®. The target gene was developed by finding the complete sequences of Sox2 nucleotides on the NCBI GenBank. The growth on LB-ampicillin agar plates was amplified by PCR to obtain colonies with pET101/D-TOPOÂ® vectors and inserts of the pluripotent gene of CSCs, then the PCR results were observed through electrophoresis. A total of fifteen colonies have DNA bands with a base pair of about 300 bp in length. The recombinant clones produced Sox2 genes with a base length of 330 bp.

Cultivation Process Optimization Of Recombinant Bacillus Subtilis Apoptin MUHAMAD SAHLAN; YUKI DESIANDINI; MUHAMMAD IQBAL; NUNUK WIDHYASTUTI; AMARILA MALIK; SISWA SETYAHADI
Microbiology Indonesia Vol. 7 No. 4 (2013): November 2013
Publisher : Indonesian Society for microbiology

The success of recombinan apoptin production in native form in the previous results open the way to develop this anticancer protein production to the larger scale. We optimized cultivation process of recombinant Bacillus subtilis 168 harbouring pOXGW12His8Arg with apoptin gene in a stirred tank fermentor and shake flasks. The parameters to optimize cultivation process are xylose-inducer concentration, agitation speed, and aeration rate. The xylose-inducer concentration variations are carried out in a shake flasks with 100 mL volume broth, while the agitation speed and aeration rate variation is carried out in a stirred tank fermentor with 3 L volume broth. The xylose concentration is varied between 0-5% w/v, while agitation speed and aeration rate are varied between 150-250 rpm and 0.5-1.5 NL min-1 respectively. The best condition in this cultivation is 1% w/v of xylose, 250 rpm of agitation speed and 1,5 NL min-1 of aeration rate giving the specific growth rate value for each parameter of 0.628 h-1; 0.630 h-1; and 0.747 h-1 respectively. The recombinant apoptin were purified by Ni-NTA column using AKTA system. However, the results showed that the optimum condition of producing recombinant apoptin was 1% w/v of xylose, 250 rpm of agitation speed, and 0.5 NL min-1 of aeration.

Angka Kejadian dan Faktor yang Memengaruhi Potensi Interaksi Obat dengan Obat pada Pasien Leukemia Akut Anak yang Menjalani Rawat Inap Sri Wulandah Fitriani; Rina Mutiara; Amarila Malik; Murti Andriastuti
Sari Pediatri Vol 18, No 2 (2016)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Latar belakang. Risiko terjadinya potensi interaksi obat dengan obat (PIOO) pada pasien leukemia akut akibat pemberian terapiÂ multidrug cukup tinggi.Tujuan. Mengetahui angka kejadian dan faktor yang memengaruhi PIOO pada subjek penelitian.Metode. Penelitian dilakukan dengan metode potong lintang. Pengambilan data dilakukan secara retrospektif terhadap pasien leukemiaÂ akut pada anak yang menjalani rawat inap di RSCM pada Januari â€“ Desember 2014. Identifikasi PIOO dilakukan menggunakanÂ perangkat lunak Micromedex Drug ReaxÂ®.Hasil. Didapat 96 subjek yang memenuhi kriteria penelitian. Subjek berpotensi mengalami PIOO 41,6% dengan tingkat keparahanÂ kontraindikasi (4,70%), berat (60,70%), dan sedang (34,60%). Jenis PIOO paling sering adalah deksametason dan flukonazolÂ yang berpotensi meningkatkan paparan glukokortikoid. Terdapat perbedaan bermakna pada faktor usia (p=0,037), lama hari rawatÂ (p=0,000), dan rerata jumlah obat (p=0,000), sedangkan komorbiditas tidak menunjukkan perbedaan (p=0,082).Kesimpulan. Angka kejadian PIOO pasien leukemia akut pada anak yang menjalani rawat inap di RSCM adalah 41,6%. FaktorÂ usia, lama hari rawat, dan rerata jumlah obat berpengaruh signifikan terhadap kejadian PIOO dengan nilai OR 1,8 terhadap pasienÂ berusia >7 tahun, 6,3 terhadap pasien dengan lama hari rawat >7 hari, dan 5,3 terhadap pasien dengan rerata pemberian >4 obat perhari. SariÂ

UGT1A1 gene polymorphisms and jaundice in Indonesian neonates Rinawati Rohsiswatmo; Radhian Amandito; Andiani Wanda Putri; Nilam Sartika; Amarila Malik
Paediatrica Indonesiana Vol 59 No 3 (2019): May 2019
Publisher : Indonesian Pediatric Society

Background Uridine diphospho-glucuronocyltransferase 1A1 (UGT1A1) polymorphisms are a risk factor for unconjugated hyperbilirubinemia in neonates. UGT1A1 polymorphisms decrease bilirubin conjugation, thus causing hyperbilirubinemia. A variety of polymorphisms have been reported, with UGT1A1*60 and UGT1A1*6 especially prominent in the Asian population. Hyperbilirubinemia polymorphism studies are lacking in Indonesian populations. Objective To identify UGT1A1*60 and UGT1A1*6 profiles in Indonesian populations of heterogeneous ethnicity. Methods We enrolled 42 jaundiced neonates who were born from January to April 2017 and treated in the Neonatal Intensive Care Unit of our national referral center, Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Genetic mutations *60 of exon 1 and *6 of the promoter region were analyzed by polymerase chain reaction â€“ restriction fragment length polymorphism methods, with DraI and AvaII as restriction enzymes, respectively. Clinical data including total serum bilirubin and racial information were obtained by medical records and interviews with parents. Results There were no homozygous mutations of UGT1A1*6, but 4.8% of subjects were heterozygous. As for UGT1A1*60, 4.8% were heterozygous and 95.2% were homozygous. Racial variations were not observed for UGT1A1*60, while Betawi descendents were found to have many heteroygous forms of UGT1A1*6. Conclusion Polymorphisms of the UGT1A1 gene were found in Indonesian neonates. Some ethnicities also showed increased tendency towards its incidence, such as the heterozygous form of UGT1A1*6.

Optimasi Perolehan DNA Mikrobioma yang Diekstraksi dari Mekonium dan Feses Neonatus Prematur untuk diaplikasikan pada Next-Gen Sequencing 16S rRNA Larashintya Rulita; Amarila Malik; Radhian Amandito; Rinawati Rohsiswatmo
JURNAL ILMU KEFARMASIAN INDONESIA Vol 19 No 2 (2021): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v19i2.1112

The composition of the intestinal microbiome of neonates can be identified from meconium and feces by Next-Generation Sequencing (NGS) technology. However, the yield of microbiome DNA of meconium and feces has its own challenges due to the consistency and the high content of PCR inhibitors in these samples. This study aims to optimize the yield of microbiome DNA from meconium sample and feces of pre-term neonates. The DNA yield was obtained by applying certain optimized parameters, i.e., considering the replication and condition of the sample, using a particular kit for DNA extraction, and modifying the DNA elution of the column purification. The genomic DNA obtained was quantified and confirmed using Polymerase Chain Reaction. Results showed that the best DNA yield was achieved by replicating the number of samples twice in the pre-extraction stage, working on fresh meconium and feces samples instead, and suspended the sample in ddH2O prior to extraction process as observed on agarose gel visualization with UV trans-illuminator, as well as in quantitative measurement by a nano spectrophotometer. The best extraction process was using MP Biomedical FastDNA Spin Kit for Soil, in addition to the use of an elution buffer in a smaller volume, resulting in a higher concentration and purity of DNA. In conclusion, we were able to obtain an optimized yet reliable DNA yields, especially from meconium, which fulfilled the quality and quantity requirement for further sequencing process of microbiome.

Optimalisasi Komposisi Koktail dan Pengembangan Proses Fermentasi Up-scale dari Empat Strain Bakteri Komensal Kulit Ahmad Baikuni; Meidy Richky Wanyodiharjo; Harry Ardiansyah; Fathan Luthfi Hawari; Amarila Malik
JURNAL ILMU KEFARMASIAN INDONESIA Vol 20 No 1 (2022): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v20i1.1224

Analyzing the actual microbial composition in the skin microbiome is riveting to create a potential sourceof active substances in skincare products. Our previous study isolated four bacterial strains from Javanese male and female skin samples, Staphylococcus hominis MBF12–19J, Staphylococcus warneri MBF02–19J, Bacillus subtilis MBF10–19J, and Micrococcus luteus MBF05–19J. This study aimed to determine the composition of those strains in a bacterial cocktail and optimize the condition for up-scale laboratory production of the cocktail. To assess the ability of bacteria to coexist and live in cocktail communities, simultaneous monitoring was performed using the Deferred Growth Inhibition Assay (DGIA) method and real-time PCR for DNA copy number measurement. Results showed the best composition based on even distribution and cell growth viability was at the ratio 1.5:1:0.5:0.5 of Micrococcus luteus MBF05-19J, Bacillus subtilis MBF10-19J, Staphylococcus warneri MBF02-19J, Staphylococcus hominis MBF12-19J, which is equivalent to DNA copy number/mL 1.209 x 1024 CFU : 2.484 x 1041 CFU : 2.645 x 1041 CFU : 9.041 x 1035 CFU, respectively. The optimum growth incubation time of individual bacterial cultures for an up-scale 2-L bio-fermentor mixture was as follows; Micrococcus luteus MBF05-19J = 21 hrs; Bacillus subtilis MBF10-19J = 7 hrs; Staphylococcus warneri MBF02-19J = 17 hrs; Staphylococcus hominis MBF12-19 = 15 hrs. For up-scale conditions, the fermentation incubation time was 3 hours at 37oC, agitation 50 RPM, and aeration 5% dissolved oxygen.

Isolasi dan Karakterisasi Protease Serupa Tripsin (PST) dari Lactobacillus plantarum FNCC 0270 TRISMILAH TRISMILAH; WAHONO SUMARYONO; AMARILA MALIK; MOHAMAD SADIKIN
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.7 KB)

Trypsin is an enzyme which is important for research as well as for the pharmaceutical industry, medicine and health, which is especially developed as a raw material for digestive enzymes. This study aimed to characterize PST from Lactobacillus plantarum FNCC 0270 that have not been studied as a producer of trypsin. Fermentation for producing PST by L . plantarum FNCC 0270 was performed in the LKB fermentor with working volume of 3.5 liters, at temperature of 37 oC, pH value of 8, agitation rate of 77 rpm and aeration of 0.5 vvm. The media composition were 3.64% baker’s yeast, 1.21% glucose, 0.13% skim milk. The fementation resulted in enzyme activities of 0.00129 U/mL and protein content of 0.49 mg/mL. Purification of the PST conducted by ultrafiltration Hollow Fiber Catridge 5 kD, presipitated with ammonium sulfate saturated (30-70%), 30 kD membrane polyesthersulfone, ion exchange column chromatography Q-XL and HiTrap affinity column chromatography. All of system increased purity of the crude PST. SDS-PAGE of PST indicated the molecular weights of 47.35; 38.42;21.40 and 12.96 kD. The PST was stable at pH value of 8 and temperature range of 25-35 oC. It is proved that the same half-life was 693.2 minutes. Km and Vmax values of the PST were 0.231 mM and 0.001 U/mL, respectively using N-α-benzoyl-DL-arginine-p-nitroanilide as the substrate. PST was inhibited by EDTA, Ca2+, Zn2+, Mg2+, Mn2+ and specific substrates. Based on the result of characterization of PST from L. plantarum FNCC 0270 can be concluded that the trypsin–like protease (PST) was trypsin.

Co-Authors 'Alawiyyah, Raden Zulfa Achmad Fariz Ahmad Baikuni Ahmad Baikuni Ahmad Faried Ajitya Kurnia Hermawati Amin Soebandrio Anandayu Nurfachtiyani ANDI YEHUDA Andiani Wanda Putri Anis Karuniawati Antonius Suwanto Arry Yanuar Asmarinah Astutiati Nurhasanah ASTUTIATI NURHASANAH Atiek Soemiati Aulia Rizki Azminah Azminah Berna Elya CESILIA ANITA Deli Marteka Dewi Puspita Donna M. Ariestanti Dumingan, Alvian Endang Saepudin Endang W Bachtiar ESTI PUSPITASARI Fairuz Andini Fatiningtyas Fathan Luthfi Hawari Fathan Luthfi Hawari Fatiningtyas, Fairuz Andini HANIFAH RAHMI Hanita Omar HANS-JOACHIM FREISLEBEN HARALD HUBER Harry Ardiansyah Hermawati, Ajitya Kurnia Hestiningtyas, Mahardhika Huda, Fathul IMAN SANTOSO INDRIANI INDRIANI Ingrid Faustine Is Helianti IS HELIANTI Islamudin Ahmad Kambang Sariadji Kino-Oka, Masahiro Kusmiati . Kusmiati Kusmiati Kusmiati Kusmiati Larashintya Rulita LILIANA SARAGIH Mahardhika Hestiningtyas Maimunah Maimunah Maksum Radji Meidy Richky Wanyodiharjo Mohamad Sadikin Muhammad Hanafi MUHAMMAD IQBAL Murti Andriastuti, Murti Nadia Farhanah Syafhan Napitupulu, Riswanto Neneng Siti Silfi Ambarwati Nilam Sartika Noverina, Rachmawati NUNUK WIDHYASTUTI Nusaiba, Putri Oktaviyani, Devi Purwaningtyas, Yoggi Ramadhani Putri, Ratih Rinendya Radhian Amandito Radhian Amandito RAFIKA SARI RAMADON, DELLY Ratih Rinendyaputri Retnaningsih, Agustina Rina Mutiara Rinawati Rohsiswatmo Riswanto Napitupulu RIZKI AYU AMALIA Rosari Saleh S.Pd. M Kes I Ketut Sudiana . SAHLAN, MUHAMAD SELA SEPTIMA MARIYA Septelia I. Wanandi Septelia Inawati W SERUNI K.U. FREISLEBEN Sheilla Sheilla SISWA SETYAHADI Sri Wulandah Fitriani Stella Vania Sukma Nuswantara Sukma Nuswantara Sunarno Sunarno Sunarno Sunarno Susan Rahayu Sutriyo Syafrida Siregar Syafrida Siregar, Syafrida Tiodinar Theresia Tri Handayani Trismilah Margono Trismilah Trismilah TRISMILAH TRISMILAH Trismilah, Utama, Hieronimus Adiyoga Nareswara Utami, Emilia Rahmadaniah Wahono Sumaryono WALTER ERDELEN Wangi Firdausi Yamamoto, Riku Yuditya Artha YUKI DESIANDINI ZESSINDA LUTHFA

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