Articles
<![CDATA[UPAYA PENYEDIAAN VIRUS MURNI UNTUK PEMBUATAN ANTISERUM Soybean Mosaic Virus ]]>
<![CDATA[Andayanie, Wuye Ria]]>
<![CDATA[ Buletin Palawija No 28 (2014) ]]>
Publisher : <![CDATA[Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian ]]>
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<![CDATA[SMV merupakan salah satu penyebab penyakit mosaik penting pada kedelai (Glycine max L. Merr.) karena dapat mengakibatkan penurunan hasil hingga 57%. Hingga saat ini SMV masih merupakan salah satu kendala bagi peningkatan produksi kedelai. Hingga saat ini, pemuliaan tanaman kedelai lebih diarahkan untuk hasil tinggi, belum ada program pemuliaan untuk ketahanan terhadap penyakit soybean mosaic virus. Pemurnian virus merupakan salah satu langkah penting untuk kajian suatu virus dan pengelolaan penyakit virus tersebut. Virus murni dapat diisolasi dari satu luka lokal (local lesion) pada Chenopodium amaranticolor yang diinokulasi SMV. Tanaman untuk perbanyakan virus (propagative plants) kebanyakan berbeda dengan tanaman inangnya. Pada SMV hanya diperbanyak pada tanaman kedelai. Salah satu faktor untuk keberhasilan pemurnian virus adalah perbanyakan virus pada tanaman inang yang tepat. Metode pemurnian sangat ditentukan oleh karakter virus. Sifat-sifat kimia fisika protein dan asam nukleat yang berbeda antara virus atau strain virus akan menyebabkan metode pemurnian virus sangat bervariasi. Virus murni ini digunakan untuk karakterisasi virus dan pembuatan antiserum. Identifikasi SMV dengan memanfaatkan reaksi antara antigen dan antibodi telah banyak diaplikasikan sebagai alat deteksi keberadaan virus pada tanaman dan evaluasi genotipe sebagai sumber gen dalam program pemuliaan untuk ketahanan terhadap SMV berdaya hasil tinggi.]]>
<![CDATA[ANTIVIRAL ACTIVITY OF CASHEW NUT SHELL EXTRACT AGAINST COWPEA MILD MOTTLE VIRUS ON SOYBEAN ]]>
<![CDATA[Andayanie, Wuye Ria]]>;
<![CDATA[Nuriana, Wahidin]]>;
<![CDATA[Ermawati, Netty]]>
<![CDATA[ JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 19 No. 2 (2019): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ]]>
Publisher : <![CDATA[Universitas Lampung ]]>
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DOI: 10.23960/j.hptt.219170-178
<![CDATA[Antiviral Activity of Cashew Nut Shell Extract Against Cowpea mild mottle virus on Soybean. Cowpea mild mottle virus (CPMMV) is one of important virus on soybean. Management of CPMMV is usually by controlling its vector using insecticide which has adverse effect on environmental and non target insect. Therefore, it is important to find an alternative control which is environment friendly such as utilizing of plant extract. Cashew nut shell (CNS) is a waste during processing of cashew nut and reported containing some antimicrobe substances. The research was aimed to evaluated the potency of CNS extract as antiviral against CPMMV. The experiment was designed by Randomized Block Design with 4 replicates and each treatment unit consisted of 10 plants. The application method (prior and post virus inoculation, mixing of virus sap and CNS extract) and concentration of CNS extract (0.75 %; 1.5 %; 3.0 %; 6.0 %) were evaluated the effectiveness of their combination to inhibit CPMMV infection. The application of CNS extract were abled to decreased disease incidence and increase incubation period compared  to untreated with CNS extract. and severity of treated plants significantly. However, plants treated with concentration 3.0 % and 6.0 % prior virus inoculation and mixing CNS extract with concentration 1.5 %, 3.0 %, and 6.0 % with CPMMV sap showed that the virus titre was negatively detected by ELISA using CPMMV antisera.  It is indicated that the potential of CNS extract as antiviral. All treatments combination able to suppress virus infection without phytotoxicity effect, except CNS 6.0 % treatment.]]>
<![CDATA[Pendampingan pada Petani Mawar Tabur di Desa Tapak Kecamatan Panekan Kabupaten Magetan ]]>
<![CDATA[Andayanie, Wuye Ria]]>
<![CDATA[ JURNAL DAYA-MAS Vol 3, No 2 (2018): JURNAL DAYA MAS ]]>
Publisher : <![CDATA[Universitas Merdeka Madiun ]]>
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DOI: 10.33319/dymas.v3i2.8
<![CDATA[Rose (Rosa hybrida L.) is classified as the genus Rosa and is an ornamental flower plant in the form of herbs with thorny stems. Roses have many types, one of which is a type of sowing roses that are widely grown in Tapak Village, Panekan Subdistrict. Sowing roses can be used as a support for daily economic needs by residents in Tapak Village. In addition, rose plants can be used as conservation plants, if planted on a bench terrace on sloping land. The Panekan area is an area declared by the Magetan Regency Government as an area prone to landslides. Until now the terraces of sloping land in Tapak Village are often not utilized with terrace reinforcing plants, so land degradation is increasing and expanding, mainly due to the high level of soil erosion, especially in sloping areas. Utilization of conservation techniques with rose plants on sloping lands prone to erosion can reduce degradation of soil fertility. Therefore it is necessary to provide assistance for: 1) planting a terrace with rose plants; 2) supply of rose seeds and non-dependence on synthetic chemical fertilizers. Community service activities are held from October to December 2018. Each partner farmer is given training and is facilitated to supply sowing roses and compost plus for nurseries. The method of activity uses a group approach, field meeting, farmer course. The mentoring activity was carried out with a pattern of rural appraisal Participatory, namely working with members of farmer groups in Tapak Village. The results of the mentoring activities were carried out to increase the income of sowing rose farmers and reduce degradation on sloping land. Keywords—: sowing roses, terrace, degradation, Tapak Village ]]>
<![CDATA[Pendampingan pada Petani Mawar Tabur di Desa Tapak Kecamatan Panekan Kabupaten Magetan ]]>
<![CDATA[Andayanie, Wuye Ria]]>;
<![CDATA[Rohmatiah, Ahadiati]]>
<![CDATA[ JURNAL DAYA-MAS Vol 4, No 2 (2019): JURNAL DAYA MAS ]]>
Publisher : <![CDATA[Universitas Merdeka Madiun ]]>
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DOI: 10.33319/dymas.v4i2.28
<![CDATA[Rose (Rosa hybrida L.) is classified as the genus Rosa and is an ornamental flower plant in the form of herbs with thorny stems. Roses have many types, one of which is a type of sowing roses that are widely grown in Tapak Village, Panekan Subdistrict. Sowing roses can be used as a support for daily economic needs by residents in Tapak Village. In addition, rose plants can be used as conservation plants, if planted on a bench terrace on sloping land. The Panekan area is an area declared by the Magetan Regency Government as an area prone to landslides. Until now the terraces of sloping land in Tapak Village are often not utilized with terrace reinforcing plants, so land degradation is increasing and expanding, mainly due to the high level of soil erosion, especially in sloping areas. Utilization of conservation techniques with rose plants on sloping lands prone to erosion can reduce degradation of soil fertility. Therefore it is necessary to provide assistance for: 1) planting a terrace with rose plants; 2) supply of rose seeds and non-dependence on synthetic chemical fertilizers. Community service activities are held from October to December 2018. Each partner farmer is given training and is facilitated to supply sowing roses and compost plus for nurseries. The method of activity uses a group approach, field meeting, farmer course. The mentoring activity was carried out with a pattern of rural appraisal Participatory, namely working with members of farmer groups in Tapak Village. The results of the mentoring activities were carried out to increase the income of sowing rose farmers and reduce degradation on sloping land.]]>
<![CDATA[UPAYA PENYEDIAAN VIRUS MURNI UNTUK PEMBUATAN ANTISERUM Soybean Mosaic Virus ]]>
<![CDATA[Wuye Ria Andayanie]]>
<![CDATA[ Buletin Palawija No 28 (2014): Buletin Palawija No 28, 2014 ]]>
Publisher : <![CDATA[Balai Penelitian Tanaman Aneka Kacang dan Umbi ]]>
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DOI: 10.21082/bulpa.v0n28.2014.p84-92
<![CDATA[SMV merupakan salah satu penyebab penyakit mosaik penting pada kedelai (Glycine max L. Merr.) karena dapat mengakibatkan penurunan hasil hingga 57%. Hingga saat ini SMV masih merupakan salah satu kendala bagi peningkatan produksi kedelai. Hingga saat ini, pemuliaan tanaman kedelai lebih diarahkan untuk hasil tinggi, belum ada program pemuliaan untuk ketahanan terhadap penyakit soybean mosaic virus. Pemurnian virus merupakan salah satu langkah penting untuk kajian suatu virus dan pengelolaan penyakit virus tersebut. Virus murni dapat diisolasi dari satu luka lokal (local lesion) pada Chenopodium amaranticolor yang diinokulasi SMV. Tanaman untuk perbanyakan virus (propagative plants) kebanyakan berbeda dengan tanaman inangnya. Pada SMV hanya diperbanyak pada tanaman kedelai. Salah satu faktor untuk keberhasilan pemurnian virus adalah perbanyakan virus pada tanaman inang yang tepat. Metode pemurnian sangat ditentukan oleh karakter virus. Sifat-sifat kimia fisika protein dan asam nukleat yang berbeda antara virus atau strain virus akan menyebabkan metode pemurnian virus sangat bervariasi. Virus murni ini digunakan untuk karakterisasi virus dan pembuatan antiserum. Identifikasi SMV dengan memanfaatkan reaksi antara antigen dan antibodi telah banyak diaplikasikan sebagai alat deteksi keberadaan virus pada tanaman dan evaluasi genotipe sebagai sumber gen dalam program pemuliaan untuk ketahanan terhadap SMV berdaya hasil tinggi.]]>
<![CDATA[SELEKSI GALUR DARI POPULASI F4 KEDELAI YANG TAHAN TERHADAP PENYAKIT MOSAIK (Soybean mosaic virus) DAN BERDAYA HASIL TINGGI ]]>
<![CDATA[Wuye Ria Andayanie]]>;
<![CDATA[Praptiningsih Gamawati Adinurani]]>
<![CDATA[ Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ]]>
Publisher : <![CDATA[Universitas Lampung ]]>
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DOI: 10.23960/j.hptt.214152-159
<![CDATA[Soybean lines selection of F4 population resistant to soybean mosaic disease (Soybean mosaic virus) with high yield. The soybean breeding program is usually not purposedly done for resistance to Soybean mosaic virus (SMV) but rather for crop yields. The experiment was aimed to obtain soybean lines of F4 population resistant to soybean mosaic disease with high yield. F2-F4 plants that have been inoculated with the T isolate of SMV one week after planting were selected by the pedigree in the screen house. The result indicated eight F4 populations (Wilis x L. Temanggung; Wilis x L. Jombang; Wilis x Pangrango; Wilis x PI 200485; Gepak Kuning x L. Jombang; Gepak Kuning x L. Temanggung; Gepak Kuning x Malabar; Gepak Kuning x PI 200485) produced medium seed size (from 9.84-10.26 g 100/seeds). Gepak Kuning x Mlg 3288 showed more resistant than Gepak Kuning x PI 200485. The seed produced by Gepak Kuning x PI 200485 was 1.97 ton/ha. There were no F4 populations that had higher yield and bigger seed size than Gepak Kuning x PI 200485 even though they were moderately resistant to SMV. Therefore, these lines of Gepak Kuning x Mlg 3288 and Gepak Kuning x PI 200485 might provide exellent sources to develop a new variety that resistant to SMV and of high yield.]]>
<![CDATA[DIAGNOSIS PENYAKIT MOSAIK (SOYBEAN MOSAIC VIRUS) TERBAWA BENIH KEDELAI ]]>
<![CDATA[Wuye Ria Andayanie]]>
<![CDATA[ Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 2 (2012): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ]]>
Publisher : <![CDATA[Universitas Lampung ]]>
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DOI: 10.23960/j.hptt.212185-191
<![CDATA[Soybean mosaic disease is wide spread throughout soybean-growing countries. Incidence of this disease in East Java is caused by Soybean mosaic virus (SMV) and cowpea mild mottle virus (CMMV). This aim of study was to find the etiology of disease at 14-28 days after planting (dap) on soybean. Research was done by observing visual symptoms. Visual symptoms confirmed by infectivity test, serology assay,electron microscopy (EM) and molecular detection. Results from experiment indicated that soybean plants (14-28 dap) with symptom could be detected in infectivity test. Mechanical inoculation with symptomatic leaf extracts produced local lessions on Chenopodium amaranticolor. Positive results were obtained for Madiun, Ngawi, and Magetan samples when tested against antiserum of SMV in serological assay, however Ponorogo samples were not detected by serological assay. Electron microscopy was also done for the selected sample to confirm the result of positive results. In EM observations, characteristic filamentous particles with modal length close to 900 nm were observed in samples infected with SMV. We detected an array of amplification products of expected size 1385 bp fragment of cylindrical inclusion gene from Madiun, Ngawi, and Magetan isolates in former fields, but not detected in Ponorogo isolate. This result showed the existence of SMV of soybean seed transmission at 14-28 dap in Madiun, Magetan and Ngawi.]]>
<![CDATA[UPAYA PENYEDIAAN VIRUS MURNI UNTUK PEMBUATAN ANTISERUM Soybean Mosaic Virus ]]>
<![CDATA[Wuye Ria Andayanie]]>
<![CDATA[ Buletin Palawija No 28 (2014): Buletin Palawija No 28, 2014 ]]>
Publisher : <![CDATA[Balai Penelitian Tanaman Aneka Kacang dan Umbi ]]>
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DOI: 10.21082/bulpa.v0n28.2014.p84-92
<![CDATA[SMV merupakan salah satu penyebab penyakit mosaik penting pada kedelai (Glycine max L. Merr.) karena dapat mengakibatkan penurunan hasil hingga 57%. Hingga saat ini SMV masih merupakan salah satu kendala bagi peningkatan produksi kedelai. Hingga saat ini, pemuliaan tanaman kedelai lebih diarahkan untuk hasil tinggi, belum ada program pemuliaan untuk ketahanan terhadap penyakit soybean mosaic virus. Pemurnian virus merupakan salah satu langkah penting untuk kajian suatu virus dan pengelolaan penyakit virus tersebut. Virus murni dapat diisolasi dari satu luka lokal (local lesion) pada Chenopodium amaranticolor yang diinokulasi SMV. Tanaman untuk perbanyakan virus (propagative plants) kebanyakan berbeda dengan tanaman inangnya. Pada SMV hanya diperbanyak pada tanaman kedelai. Salah satu faktor untuk keberhasilan pemurnian virus adalah perbanyakan virus pada tanaman inang yang tepat. Metode pemurnian sangat ditentukan oleh karakter virus. Sifat-sifat kimia fisika protein dan asam nukleat yang berbeda antara virus atau strain virus akan menyebabkan metode pemurnian virus sangat bervariasi. Virus murni ini digunakan untuk karakterisasi virus dan pembuatan antiserum. Identifikasi SMV dengan memanfaatkan reaksi antara antigen dan antibodi telah banyak diaplikasikan sebagai alat deteksi keberadaan virus pada tanaman dan evaluasi genotipe sebagai sumber gen dalam program pemuliaan untuk ketahanan terhadap SMV berdaya hasil tinggi.]]>
<![CDATA[Pendampingan pada Petani Mawar Tabur di Desa Tapak Kecamatan Panekan Kabupaten Magetan ]]>
<![CDATA[Wuye Ria Andayanie]]>
<![CDATA[ JURNAL DAYA-MAS Vol. 3 No. 2 (2018): JURNAL DAYA MAS ]]>
Publisher : <![CDATA[Universitas Merdeka Madiun ]]>
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DOI: 10.33319/dymas.v3i2.8
<![CDATA[Rose (Rosa hybrida L.) is classified as the genus Rosa and is an ornamental flower plant in the form of herbs with thorny stems. Roses have many types, one of which is a type of sowing roses that are widely grown in Tapak Village, Panekan Subdistrict. Sowing roses can be used as a support for daily economic needs by residents in Tapak Village. In addition, rose plants can be used as conservation plants, if planted on a bench terrace on sloping land. The Panekan area is an area declared by the Magetan Regency Government as an area prone to landslides. Until now the terraces of sloping land in Tapak Village are often not utilized with terrace reinforcing plants, so land degradation is increasing and expanding, mainly due to the high level of soil erosion, especially in sloping areas. Utilization of conservation techniques with rose plants on sloping lands prone to erosion can reduce degradation of soil fertility. Therefore it is necessary to provide assistance for: 1) planting a terrace with rose plants; 2) supply of rose seeds and non-dependence on synthetic chemical fertilizers. Community service activities are held from October to December 2018. Each partner farmer is given training and is facilitated to supply sowing roses and compost plus for nurseries. The method of activity uses a group approach, field meeting, farmer course. The mentoring activity was carried out with a pattern of rural appraisal Participatory, namely working with members of farmer groups in Tapak Village. The results of the mentoring activities were carried out to increase the income of sowing rose farmers and reduce degradation on sloping land. Keywords—: sowing roses, terrace, degradation, Tapak Village ]]>
<![CDATA[Pendampingan pada Petani Mawar Tabur di Desa Tapak Kecamatan Panekan Kabupaten Magetan ]]>
<![CDATA[Wuye Ria Andayanie]]>;
<![CDATA[Ahadiati Rohmatiah]]>
<![CDATA[ JURNAL DAYA-MAS Vol. 4 No. 2 (2019): JURNAL DAYA MAS ]]>
Publisher : <![CDATA[Universitas Merdeka Madiun ]]>
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DOI: 10.33319/dymas.v4i2.28
<![CDATA[Rose (Rosa hybrida L.) is classified as the genus Rosa and is an ornamental flower plant in the form of herbs with thorny stems. Roses have many types, one of which is a type of sowing roses that are widely grown in Tapak Village, Panekan Subdistrict. Sowing roses can be used as a support for daily economic needs by residents in Tapak Village. In addition, rose plants can be used as conservation plants, if planted on a bench terrace on sloping land. The Panekan area is an area declared by the Magetan Regency Government as an area prone to landslides. Until now the terraces of sloping land in Tapak Village are often not utilized with terrace reinforcing plants, so land degradation is increasing and expanding, mainly due to the high level of soil erosion, especially in sloping areas. Utilization of conservation techniques with rose plants on sloping lands prone to erosion can reduce degradation of soil fertility. Therefore it is necessary to provide assistance for: 1) planting a terrace with rose plants; 2) supply of rose seeds and non-dependence on synthetic chemical fertilizers. Community service activities are held from October to December 2018. Each partner farmer is given training and is facilitated to supply sowing roses and compost plus for nurseries. The method of activity uses a group approach, field meeting, farmer course. The mentoring activity was carried out with a pattern of rural appraisal Participatory, namely working with members of farmer groups in Tapak Village. The results of the mentoring activities were carried out to increase the income of sowing rose farmers and reduce degradation on sloping land.]]>
