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Muhammad Diki Juliandi
Universitas Perintis Indonesia
Earth & Planetary Sciences Environmental Science

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Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

Abstract

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms
Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

Abstract

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms
Dampak Paparan Logam Berat Besi (Fe) Terhadap Hemoglobin (Hb) Pada Darah Tikus Wistar Dewi Yudiana Shinta; Muhammad Diki Juliandi; Def Primal
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.111

Abstract

Iron (Fe) heavy metal, in certain amounts is an essential heavy metal, much needed by living organisms; however, in excessive amounts, iron metal can be toxic. Iron (Fe) metal has adverse effects on health, as it is harmful to organs and causes physiological problems especially Hb levels in the blood. This study aims to see the impact of exposure to heavy metal iron (Fe) on living things represented by Wistar rats. The benefit of this study is to provide an overview of the exposure of living things, especially humans, will have an impact on hemoglobin levels that make humans can be affected by anemia. This research design with a post test group design only with a total sample of 10 rats divided into 3 groups, namely negative control, treatment 1 with a dose of 0.54 mg / BW and treatment 2 with a dose of 1.08 mg/BW, giving (Fe) is given from the first day to the 14th day and then taking rat blood through the orbital eye. Examination of heavy metal Fe with atomic absorption spectrophotometry and for Hb levels with Hemosmart Gold Hemoglobin Test Strip. The results of this study heavy metal (Fe) levels in the blood of rats are the highest levels found in the sample 1.08mg / BW T3 (H14) with Fe levels of 44.14 µg / L and the lowest Fe levels found in the sample with Fe levels of 14.78 µg / L and control of rats, Fe levels are 9.85 µg / L. Hb levels in the blood of rats were 0.54mg/BB dose was (7.4 g/dl and 6.8g/dl), 1.08mg/BB dose obtained hemoglobin results (7.2g/dl and 7.6g/dl). The normal Hb level of control rats in rat blood is 6.1 g/dL. The conclusion of this study is that the level of Fe that enters the body of rats greatly affects the hemoglobin (Hb) of wistar rats where if the level of heavy metal Fe increases in the blood there will be a decrease in hemoglobin (Hb) levels in the blood which will cause anemia.
Dampak Paparan Logam Berat Besi (Fe) Terhadap Hemoglobin (Hb) Pada Darah Tikus Wistar Dewi Yudiana Shinta; Muhammad Diki Juliandi; Def Primal
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.111

Abstract

Iron (Fe) heavy metal, in certain amounts is an essential heavy metal, much needed by living organisms; however, in excessive amounts, iron metal can be toxic. Iron (Fe) metal has adverse effects on health, as it is harmful to organs and causes physiological problems especially Hb levels in the blood. This study aims to see the impact of exposure to heavy metal iron (Fe) on living things represented by Wistar rats. The benefit of this study is to provide an overview of the exposure of living things, especially humans, will have an impact on hemoglobin levels that make humans can be affected by anemia. This research design with a post test group design only with a total sample of 10 rats divided into 3 groups, namely negative control, treatment 1 with a dose of 0.54 mg / BW and treatment 2 with a dose of 1.08 mg/BW, giving (Fe) is given from the first day to the 14th day and then taking rat blood through the orbital eye. Examination of heavy metal Fe with atomic absorption spectrophotometry and for Hb levels with Hemosmart Gold Hemoglobin Test Strip. The results of this study heavy metal (Fe) levels in the blood of rats are the highest levels found in the sample 1.08mg / BW T3 (H14) with Fe levels of 44.14 µg / L and the lowest Fe levels found in the sample with Fe levels of 14.78 µg / L and control of rats, Fe levels are 9.85 µg / L. Hb levels in the blood of rats were 0.54mg/BB dose was (7.4 g/dl 6.8g/dl), 1.08mg/BB dose obtained hemoglobin results (7.2g/dl 7.6g/dl). The normal Hb level of control rats in rat blood is 6.1 g/dL. The conclusion of this study is that the level of Fe that enters the body of rats greatly affects the hemoglobin (Hb) of wistar rats where if the level of heavy metal Fe increases in the blood there will be a decrease in hemoglobin (Hb) levels in the blood which will cause anemia. 
Co-Authors Akmal Djamaan Anthoni Agustien Def Primal Dewi Yudiana Shinta