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Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms

Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms

Dampak Paparan Logam Berat Besi (Fe) Terhadap Hemoglobin (Hb) Pada Darah Tikus Wistar Dewi Yudiana Shinta; Muhammad Diki Juliandi; Def Primal
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.111

Iron (Fe) heavy metal, in certain amounts is an essential heavy metal, much needed by living organisms; however, in excessive amounts, iron metal can be toxic. Iron (Fe) metal has adverse effects on health, as it is harmful to organs and causes physiological problems especially Hb levels in the blood. This study aims to see the impact of exposure to heavy metal iron (Fe) on living things represented by Wistar rats. The benefit of this study is to provide an overview of the exposure of living things, especially humans, will have an impact on hemoglobin levels that make humans can be affected by anemia. This research design with a post test group design only with a total sample of 10 rats divided into 3 groups, namely negative control, treatment 1 with a dose of 0.54 mg / BW and treatment 2 with a dose of 1.08 mg/BW, giving (Fe) is given from the first day to the 14th day and then taking rat blood through the orbital eye. Examination of heavy metal Fe with atomic absorption spectrophotometry and for Hb levels with Hemosmart Gold Hemoglobin Test Strip. The results of this study heavy metal (Fe) levels in the blood of rats are the highest levels found in the sample 1.08mg / BW T3 (H14) with Fe levels of 44.14 µg / L and the lowest Fe levels found in the sample with Fe levels of 14.78 µg / L and control of rats, Fe levels are 9.85 µg / L. Hb levels in the blood of rats were 0.54mg/BB dose was (7.4 g/dl and 6.8g/dl), 1.08mg/BB dose obtained hemoglobin results (7.2g/dl and 7.6g/dl). The normal Hb level of control rats in rat blood is 6.1 g/dL. The conclusion of this study is that the level of Fe that enters the body of rats greatly affects the hemoglobin (Hb) of wistar rats where if the level of heavy metal Fe increases in the blood there will be a decrease in hemoglobin (Hb) levels in the blood which will cause anemia.

Dampak Paparan Logam Berat Besi (Fe) Terhadap Hemoglobin (Hb) Pada Darah Tikus Wistar Dewi Yudiana Shinta; Muhammad Diki Juliandi; Def Primal
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.111

Iron (Fe) heavy metal, in certain amounts is an essential heavy metal, much needed by living organisms; however, in excessive amounts, iron metal can be toxic. Iron (Fe) metal has adverse effects on health, as it is harmful to organs and causes physiological problems especially Hb levels in the blood. This study aims to see the impact of exposure to heavy metal iron (Fe) on living things represented by Wistar rats. The benefit of this study is to provide an overview of the exposure of living things, especially humans, will have an impact on hemoglobin levels that make humans can be affected by anemia. This research design with a post test group design only with a total sample of 10 rats divided into 3 groups, namely negative control, treatment 1 with a dose of 0.54 mg / BW and treatment 2 with a dose of 1.08 mg/BW, giving (Fe) is given from the first day to the 14th day and then taking rat blood through the orbital eye. Examination of heavy metal Fe with atomic absorption spectrophotometry and for Hb levels with Hemosmart Gold Hemoglobin Test Strip. The results of this study heavy metal (Fe) levels in the blood of rats are the highest levels found in the sample 1.08mg / BW T3 (H14) with Fe levels of 44.14 µg / L and the lowest Fe levels found in the sample with Fe levels of 14.78 µg / L and control of rats, Fe levels are 9.85 µg / L. Hb levels in the blood of rats were 0.54mg/BB dose was (7.4 g/dl 6.8g/dl), 1.08mg/BB dose obtained hemoglobin results (7.2g/dl 7.6g/dl). The normal Hb level of control rats in rat blood is 6.1 g/dL. The conclusion of this study is that the level of Fe that enters the body of rats greatly affects the hemoglobin (Hb) of wistar rats where if the level of heavy metal Fe increases in the blood there will be a decrease in hemoglobin (Hb) levels in the blood which will cause anemia.

Studi Kesehatan Lingkungan Terhadap Penderita Tuberkulosis Paru yang Mengkonsumsi Obat Anti Tuberkulosis (OAT) Dewi Yudiana Shinta; Muhammad Diki Juliandi; Melly Siska Suryani; Ikhwan Resmala Sudji; Merry Thressia; Herix Sonata MS
SEHATI: Jurnal Kesehatan Vol 5, No 2 (2025)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/sehati.v5i2.121

Pulmonary tuberculosis (TB) is an infectious disease that requires long-term treatment using anti-tuberculosis drugs (OAT). The use of OAT, especially in the long term, can cause side effects on metabolism and organ function, one of which is indicated by changes in uric acid and urea levels in the blood. This study aims to analyze the relationship between environmental health and uric acid and urea levels in pulmonary TB patients undergoing OAT therapy. The benefits of this research include examining the relationship between uric acid and urea levels in pulmonary TB patients taking anti-tuberculosis drugs (OAT) and informing patients that TB treatment may cause an increase in uric acid and urea levels in the kidneys. The study design is an analytical observational study. The cross-sectional design involves measuring uric acid and urea levels using a chemical analyzer. The results of uric acid level measurements using the chemical analyzer showed that 25 respondents had normal uric acid levels (72%), while 28% had levels above the normal range. For urea levels, 16 respondents (64%) had normal values, and 9 respondents (36%) had levels exceeding the normal range. After consuming OAT for 1 month or less, 9 patients (60%) were observed, 2–3 months for 2 patients (13.33%), and 5 months for 1 patient (6.67%).

Studi Toksikologi Lingkungan: Pengaruh Paparan Mikroplastik Terhadap Jumlah Leukosit Tikus Putih (Rattus norvegicus) Dewi Yudiana Shinta; Melly Siska Suryani; Ikhwan Resmala Sudji; Muhammad Diki Juliandi; Merry Thressia; Herix Sonata MS
SEHATI: Jurnal Kesehatan Vol 6, No 1 (2026)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/sehati.v6i1.183

Increasing microplastic pollution in the environment raises concern about toxic risks to living organisms, and one important health risk involves immune system disruption, which can be observed through changes in leukocyte counts. This study aims to analyze the effect of microplastic exposure on leukocyte numbers in white rats (Rattus norvegicus) as an early indicator of environmental toxicity and health risk. The research also seeks to provide scientific evidence that can support environmental management policies and human health protection strategies. An experimental design was applied in which rats were divided into one control group and several treatment groups, and each treatment group received oral microplastic exposure at different doses over a specific period while FTIR spectroscopy (Fourier Transform Infrared Spectroscopy) was used to characterize the microplastic particles before administration. After the exposure period, blood samples were collected and leukocyte counts were measured using a hematology analyzer, and statistical analysis was then performed to compare leukocyte levels across all groups. The results showed that rats exposed to microplastics exhibited increased leukocyte counts above the normal range during both week one and week two, and these elevated counts were observed across groups that received different doses of microplastics. The rise in leukocyte numbers indicates an inflammatory response and immunological stress caused by microplastic exposure, and furthermore, the findings suggest that microplastics not only produce toxic effects but also tend to accumulate and remain in the body for extended periods. In conclusion, microplastic exposure has a significant effect on leukocyte counts in white rats, and this effect reflects immune system disturbance and potential disruption of immunological homeostasis. Consequently, microplastics should be recognized as an important threat in environmental toxicology, and continued research and regulatory attention are necessary to address the risks that microplastics pose to both ecological systems and human health

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