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All Journal Acta Biochimica Indonesiana
Siska Alicia Farma
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatera, Indonesia. 2Center of Research on Recycling Organic Waste Management, Universitas Negeri Padang
Biochemistry, Genetics & Molecular Biology

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The potential of local orange peel-derived eco-enzymes in producing indole acetic acid Siska Alicia Farma; Nurfa Dewiza Luzik; Salma Sakina; Irma Leilani Eka Putri; Linda Advinda; Azwir Anhar
Acta Biochimica Indonesiana Vol. 6 No. 2 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.135

Abstract

Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture. Objective: This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones. Methods: The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method. Results: ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL). Conclusion: Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture.
Co-Authors Azwir Anhar Irma Leilani Eka Putri Linda Advinda Nurfa Dewiza Luzik Salma Sakina