Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Jeumpa
Fatiha, Fathma Dwi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Variasi Genetik Prion Protein Gene pada Kucing Domestik (Felis catus) Menggunakan Metode RFLP In Silico Pinta, Sari Rahma; Pratama, Chelsylia Dara; Fatiha, Fathma Dwi; Muharani, Silvia; Achyar, Afifatul
Jurnal Jeumpa Vol 11 No 1 (2024): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v11i1.9933

Abstract

FSE adalah penyakit neurodegeneratif yang disebabkan oleh prion yang mempengaruhi semua keluarga kucing. Penelitian ini bertujuan untuk mengidentifikasi variasi genetik dalam prion protein gene pada kucing domestik (Felis catus) menggunakan RFLP In silico. Penelitian ini merupakan penelitian deskriptif yang menyajikan karakteristik dari sampel yang diuji. Sampel yang digunakan yaitu sekuen prion protein gene dalam format fasta dari NCBI, dengan nomor identitas NCBI Popset 2445476754. Skrining enzim restriksi dilakukan menggunakan situs insilico.ehu.es. Metode RFLP in silico ini dilakukan menggunakan tools pada situs benchling.com dengan enzim restriksi yang diperoleh selama tahap skrining. Data yang telah didapatkan dianalisis secara deskriptif. Enzim restriksi yang digunakan yaitu enzim HhaI dengan sisi pemotongan pada basa G_CG'C dan enzim DpnI dengan sisi pemotongan pada GA'TC. Hasil restriksi dengan enzim HhaI pada 25 isolat prion protein gene Felis catus menghasilkan 4 variasi alel yaitu alel A1 (frekuensi alel 0,08), alel A2 (frekuensi alel 0,16), alel A3 (frekuensi alel 0,68) dan alel A4 (frekuensi alel 0,08). Restriksi yang dilakukan oleh enzim DpnI menghasilkan 2 alel yaitu alel B1(frekensi alel 0,84) dan alel B2 (frekuensi alel 0,16). Hasil penelitian ini dapat digunakan sebagai rujukan awal penelitian tentang gen Prion kucing domestik pada tahap yang lebih kompleks seperti bioinformatika, maupun pada tahap kerja laboratorium.
Innovation in Bromelain Enzyme Production from Pineapple and Honey and Its Characteristics Yulastri, Weni; Irdawati, Irdawati; Yusrizal, Yusrizal; Fatiha, Fathma Dwi; Nissha, Dwi Khayrun; Anggraeni, Novicka Putri
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.6976

Abstract

Bromelain enzyme is one type of protease enzyme that has the ability to hydrolyze protein peptide bonds into amino acids. Bromelain enzyme can be found in pineapple (Ananas comosus) which consists of 95% cysteine protease mixture. Bromelain is often used by athletes to treat physical injuries or minor wounds. In obtaining the bromelain enzyme found in pineapple, an isolation process is required, namely by extracting it extracellularly. The method in this study does not go through the cell breakdown process so that it is easier to separate from other impurities and does not mix with other cell materials. This study aims to develop an innovative method in the production of bromelain enzyme using pineapple and honey as raw materials and to evaluate the organoleptic properties, pH, and the presence of lactic acid bacteria. This study is a descriptive study presented in the form of graphs and images. From the research that has been done, it can be concluded that the manufacture of bromelain enzyme using pineapple and honey has succeeded in producing a product that has an organoleptic value of liking (60%), pH 3 (acid) with the number of lactic acid bacteria 2.43 cells/ml.
Co-Authors Achyar, Afifatul Anggraeni, Novicka Putri Irdawati Irdawati Muharani, Silvia Nissha, Dwi Khayrun Pinta, Sari Rahma Pratama, Chelsylia Dara Weni Yulastri Yusrizal Yusrizal