Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Bumi Lestari
Zaetuna, Hanifa Laila
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Energy Environmental Science

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Optimasi PCR (Polymerase Chain Reaction) dan Identifikasi Ikan Hiu Muda (Juvenile) dengan Metode DNA Barcoding Zaetuna, Hanifa Laila; Sembiring, Andrianus; Pharmawati, Made
Bumi Lestari Journal of Environment Vol 24 No 2 (2024)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843//blje.2024.v24.i02.p01

Abstract

Polymerase Chain Reaction (PCR) adalah suatu teknik sintesis dan amplifikasi DNA secara in-vitro yang digunakan untuk identifikasi hewan, tumbuhan maupun mikroorganisme dengan DNA barcoding. Identifikasi secara molekular menggunakan metode berbasis PCR perlu dilakukan pada ikan hiu muda (juvenile) karena belum terlihatnya karakter kunci untuk identifikasi ke tingkat spesies sehingga identifikasi berbasis morfologi sangat sulit dilakukan. Selain itu, kondisi demikian juga tidak mendukung kelestarian ikan hiu, karena dalam fase ini hiu belum memiliki kesempatan memijah sekali dalam siklus hidupnya. Penelitian ini bertujuan untuk menentukan kondisi optimal untuk amplifikasi DNA dan mengidentifikasi ikan hiu muda (juvenile) menggunakan metode DNA Barcoding. Amplifikasi dilakukan pada lokus cytochrome oxidase subunit I (COI) dengan modifikasi temperatur suhu pada tahap annealing. Hasil penelitian menunjukkan modifikasi amplifikasi dapat dilakukan dengan penggantian temperatur suhu annealing. Hasil PCR terbaik diperoleh pada suhu annealing 55oC. Hasil identifikasi ikan hiu muda (juvenile) yang didaratkan di Pelabuhan Perikanan Pantai (PPP) Muncar dengan metode DNA Barcoding pada gen COI adalah Prionace glauca.
Keanekaragaman Jenis Burung di Kawasan Kampus Universitas Udayana, Bukit Jimbaran, Bali Sharon, Michelle Angelina; Sumbawati, Ni Kadek Aryani; Andani, Firman; Zaetuna, Hanifa Laila; Wijaya, I Made Saka; Yuni, Luh Putu Eswaryanti Kusuma
Bumi Lestari Journal of Environment Vol 24 No 1 (2024)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2024.v24.i01.p06

Abstract

Birds are an important component of balancing ecosystems and have a reciprocal relationship and close dependence to their environment. The high number of bird species found can indicate the ecological role of the environment, either for finding food and shelter, or for nesting and breeding areas. Udayana University Bukit Jimbaran Campus, with a total land area of ??27,649 ha, still has plenty of green area. This condition provides great potential as habitat for various bird species. This study aims to identify the bird species found in Udayana University Bukit Jimbaran campus area. The method used was the point count method at 11 observation points that was determined by purposive sampling. The indices calculated were the diversity, uniformity, dominance, and relative abundance. From this study, 30 bird species from 18 families were recorded with a total of 571 individuals scattered at each observation point. Based on the calculated indices, the diversity of bird species in Bukit Jimbaran Udayana University campus area was considered as moderate, with a stable species distribution, and low dominance (no species dominates). Keywords: abundance, birds, diversity, dominance, evenness
Co-Authors Andani, Firman Andrianus Sembiring, Andrianus LUH Puru ESWARYANTI KUSUMA YUNI MADE PHARMAWATI Sharon, Michelle Angelina Sumbawati, Ni Kadek Aryani Wijaya, I Made Saka