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All Journal Jurnal Sains dan Kesehatan Jurnal Nurse
Suci Indah Budiarti
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Health Professions Nursing Public Health

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Pemeriksaan Sputum Basil Tahan Asam (BTA) pada Pasien Suspek Tuberculosis dengan Metode Ziehl Nelseen Epsi Marwati; Oksita Asri Widyayanti; Suci Indah Budiarti; Delita Septining Tyas
Jurnal Nurse Vol. 4 No. 2 (2021): Juli : Jurnal Nurse
Publisher : STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/nurse.v4i2.231

Abstract

The infectious disease tuberculosis is transmitted through the air by Mycobacterium tuberculosis when an infected patient coughs, sneezes or talks. Indonesia is the third country among the countries that contribute to 60% of the world's TB cases. Diagnosis of TB can be done by detecting the presence of acid-fast bacilli (BTA) Mycobacterium tuberculosis in sputum examination. The Ziehl Neelsen method of BTA sputum examination has quite high sensitivity in detecting pulmonary TB. The aim of the study was to identify BTA sputum in patients with suspected pulmonary tuberculosis using the Ziehl Nelseen method. The research was carried out observationally through the BTA examination stage by taking sputum, laboratory checks and interpreting the results using the IUATED scale. The results of the BTA examination showed that patients who were positive for TB had positive BTA results at all three examination times. Acid-fast bacilli in TB positive patients are red after carbol fuchsin staining using the Ziehl Nelseen method. More male patients get TB than women, in this study, namely 33%. Patients who are positive for TB are aged 35 – 44 years. The conclusion of this study is the results of identification of BTA sputum from TB positive patients which shows a red color on carbol fuchsin staining using the Ziehl Nelseen method.
Efek Pemberian Ekstrak Jamur Shiitake (Lentinula Edodes) Terhadap Aktivitas Superoksida Dismutase Pada Tikus Yang Diinduksi Karbon Tetraklorida Cici Farhana Ambarwanty Mohtar; Suci Indah Budiarti
Jurnal Nurse Vol. 4 No. 1 (2021): Januari : Jurnal Nurse
Publisher : STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/nurse.v4i1.233

Abstract

Shiitake mushrooms (Lentinula edodes) is edible mushrooms and medicinal mushrooms because they contain high nutrients and bioactive compounds, such as flavonoids, phenols and terpenoids that can function as antioxidants. Antioxidants are molecules that are able to ward off free radicals in the body, although naturally the body can ward off free radicals through enzymatic (endogenous) antioxidants, such as superoxide dismutase (SOD). But if too many free radicals enter the body, it can cause oxidative stress so that endogenous antioxidants cannot work properly, so exogenous antioxidants are needed such as those found in ethanol extracts of L. edodes. One of the materials that can become free radicals is carbon tetrachloride (CCl4). This study aims to determine the effect of ethanol extract of L.edodes on levels of SOD, in rats and determine the most effective dose of ethanol extract on increasing levels of SOD and blood catalase in rats induced by CCl4. This research carried out experimentally with a Completely Randomized Design (CRD) with variation of ethanol extract concentration (250; 500; 750 ppm). The results showed that the extraction of shiitake mushroom extract has an effect on increase in the levels of superoxide dismutase and catalase in rats induced carbon tetrachloride. The concentration of ethanol extract of 500mg/kg BW of rats was the most effective concentration for increasing superoxide dismutase and the ethanolic extract of L. edodes had antioxidant activity.
Potensi Bakteri Tanah Penghasil Senyawa Antimikrob Untuk Menghambat Pertumbuhan Bakteri Escherichia Coli dan Staphylococcus Aureus Mazidah Noer Inayah; Suci Indah Budiarti
Jurnal Nurse Vol. 4 No. 1 (2021): Januari : Jurnal Nurse
Publisher : STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/nurse.v4i1.234

Abstract

Antimicrobials are molecules that inhibit the growth of microbes. Some bacterial species, consisting of actinomycetes and fungi, are capable of producing antimicrobial substances. Streptomyces, commonly referred to as soil bacteria, are a group of actinomycetes that produce multiple antimicrobial agents. Other soil bacteria that may produce antibiotics include Pseudomonas, Bacillus, Nocardia, Kibdelosporangium, and Roseobacter. Thereby, soil is one of the potential sources that is capable of being used as a research sample for discovering and collecting antibiotic-producing bacteria, especially new types of antibiotics that weren't previously discovered. The latest research about antimicrobials (antibiotics) has been driven by the high number of cases of bacterial resistance. The study aims to investigate the potential of antimicrobial-producing bacteria from the soil to inhibit the pathogenic bacteria Staphylococcus aureus and Escherichia coli. The research procedure involves soil sampling, isolating the antimicrob-producing bacteria, describing the morphology of the bacterial colony and bacterial cells, purifying and regenerating the bacterial culture, and testing the activity of antimicrobial substances against E. coli and S. aureus. The study revealed four bacterial isolates: E1, E2, S1, and S2, that could possibly suppress the growth of S. aureus and E. coli. However, the antimicrobial activity of the four bacteria remained considerably smaller compared with 100 mg of chloramphenicol.
Co-Authors Cici Farhana Ambarwanty Mohtar Delita Septining Tyas Epsi Marwati Esa Dhiandani Inayah, Mazidah Noer Martha Intan Nagari Pratiwi Oksita Asri Widyayanti