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IDENTIFIKASI PEWARNA RHODAMIN B PADA PERONA MATA DENGAN KROMATOGRAFI LAPIS TIPIS Indra, Febryan Feriza; Jayadi, Lukky; Ikayanti, Retno
Jurnal Insan Farmasi Indonesia Vol 7 No 2 (2024): JIFI : Special edition
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v7i2.2087

Abstract

Eyeshadow is a cosmetic that contains color pigments derived from natural/inorganic ingredients. The results of the BPOM survey conducted in 2021 to 2022 found that 16 sample products of blush on, nail polish, lip balm, eyeshadow and lipstick did not meet the requirements, containing prohibited ingredients, Rhodamine B. Considering the high use of cosmetics by the public and the report of eyeshadows that contain Rhodamine B, then it is necessary toidentify the Rhodamine B content in the eyeshadow. The aim of this study was to identify Rhodamine B in eyeshadow samples using Thin Layer Chromatography (TLC). The samples used were 4 different brands without BPOM registration numbers, consisting of 10 pallets, with pink to dark red colors. Identification was carried out using the TLC method with the mobile phase n-butanol: ethyl acetate: 25% ammonia (55:20:25). The test was carried out in 2 replications with the test results showing that 3 out of 10 samples showed the color and the Rf value that was in line with the Rf 0.63 of Rhodamine B standard. Further analysis was carried out by applying the UV light at 254 nm and 366 nm to the plate, and showed a typical fluorescence of Rhodamine B. 
Analysis of Caffeine, Tannin and Total Phenol Content of Tea Leaves from Sirah Kencong Blitar Plantation Dikatoru, Ravendha; Widayanti, Elok; Ikayanti, Retno
Indonesian Journal of Chemical Research Vol 12 No 2 (2024): Edition for September 2024
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2024.12-rav

Abstract

Caffeine, tannins, and total phenol are chemical components of tea leaves that are beneficial for the body. Caffeine is a psychoactive substance, tannin is anti-diarrhea, and phenol compounds are antioxidants that prevent free radicals. The purpose of this research was to analyze the levels of caffeine, tannin, and total phenol of tea leaves at Sirah Kencong Blitar Plantation. Determination of the caffeine level was carried out in tea leaves powder using UV-Vis Spectrophotometry method at 273.5 nm wavelength with caffeine as a standard. The determination of tannin and total phenol levels was carried out using the ethanol extract of tea leaves. Tannin was determined by the Follin Denis method using UV-Vis spectrophotometry at 649.9 nm wavelength with tannic acid as a standard. The total phenol was determined by the Follin Ciocalteu method using UV-Vis spectrophotometry at 794 nm wavelength with gallic acid as a standard. Based on the results of the research in tea leaves from the Sirah Kencong Blitar Plantation, the caffeine content was 1.649%, tannin was 7.095%, and total phenol was 34.142%.
Development of Banana Kepok Starch Extract (Musa Pardisia) and Moringa Leaves (Moringa oleifera Lamk.) As An Adolescent Prebiotic Supplement Luthfiyah, Fifi; Ikayanti, Retno; Iswarawanti, Dwi Nastiti
Asian Journal of Social and Humanities Vol. 2 No. 9 (2024): Asian Journal of Social and Humanities
Publisher : Pelopor Publikasi Akademika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59888/ajosh.v2i9.330

Abstract

The study aimed to determine the feasibility of kepok banana starch, Moringa leaf powder, to meet microbiological requirements as a high fe-resistant starch supplement Methode. This type of research is experimental research. The study was conducted in two stages. The first stage determines the formulation of resistant starch extract supplements to which Moringa leaf powder is added. The second stage determines the composition of vitamin C levels, fiber content, and chemical composition of the product. The third stage analyzes the product stability test by differentiating products based on storage duration and storage temperature. In this study, the type of banana used was the red kepok banana which calculated water extraction. Results: 6,679 kg of fresh kepok bananas produced 840 g of resistant starch. The yield of the material is 14.97%. 1,580 kg of fresh Moringa leaves produce 587 grams of Moringa leaf powder. material yield of 37.15%. The use of the calculated supplement is 11 grams of banana starch extract mixed with 0.5 grams of Moringa powder. The results of the microbiological examination that has been carried out on product samples show that the product is free and safe from E-coli, Staphylococcus, and Salmonella thypi bacteria and has met the microbiological quality requirements for health supplement products. Conclusion: There are three microbiological examinations that have been carried out on product samples, namely Salmonella Sp, E-Colli, and Staphylococcus Aureus, and have met the microbiological quality requirements of health supplement products.