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All Journal HAYATI Journal of Biosciences Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Current Research on Biosciences and Biotechnology OKTAL : Jurnal Ilmu Komputer dan Sains BULLET : Jurnal Multidisiplin Ilmu
Abinawanto
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Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Electrical & Electronics Engineering Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Social Sciences

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Hormonal and Laboratory Profile of Polycystic Ovarian Syndrome (Pcos) Women Visiting Fertility Center in Indonesia Sundari, Ayu Mulia; Wulandari, Tri Aprilliana; Prakoso, Nurul Muhammad; Dwiranti, Astari; Abinawanto; Boediono, Arief; Funahashi, Hiroaki; Bowolaksono, Anom
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 18 No 1 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v18i1.7546

Abstract

Our study aims to scrutinize the hormonal and laboratory profile of women with PCOS visiting a fertility center in Indonesia. A computerized database of 120 PCOS women and 120 controls was retrospectively reviewed and analyzed. According to analysis, the concentration of AMH and basal LH were significantly more elevated in the PCOS group than in the control group (6.77 vs. 2.05, P<0.001; and 6.8 vs. 5.3, P<0.001, respectively). The level of basal FSH was significantly lower in the PCOS group (5.83 vs. 7.86; P<0.001). After an IVF cycle, PCOS women had significantly higher retrieved oocytes and mature oocytes compared to controls (18 vs. 8, P<0.001; 12 vs. 6, P<0.001). The number of fertilizations, cleavages, and top-quality cleavages were also higher in the PCOS group than in the control group (10 vs. 4, P<0.001; 10 vs. 4, P<0.001; 4 vs. 2, P<0.001). At the blastocyst stage, patients with PCOS had a higher number of blastocysts and top-quality blastocysts than control (9 vs. 4, P<0.001); 4 vs. 1, P<0.01). However, the clinical pregnancy was significantly higher in the control group than in the PCOS group (52.6% vs. 16.7%; P = 0.045).
Pengaruh Pemberian Kuning Telur Terhadap Viabilitas Sperma Ikan Lukas (Puntius bramoides Val) Pascakriopreservasi Primasari Pertiwi; Abinawanto
OKTAL : Jurnal Ilmu Komputer dan Sains Vol 3 No 11 (2024): OKTAL : Jurnal Ilmu Komputer Dan Sains
Publisher : CV. Multi Kreasi Media

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Abstract

The research aims to determine the effect of several concentrations of egg yolk as an effective natural cryoprotectant for cryopreservation and to analyze the quality of lukas fish spermatozoa (Puntius bramoides Val) 48 hours after cryopreservation. This research used a 9 treatments and 4 replications. The concentration of egg yolk used as a cryoprotectant was 0% (negative control), 5%, 7%, 9%, 11%, 13%, 15%, 17%, and 1% Carboxymethyl Cellulose (CMC) was used as a positive control. The results showed that the use of several concentrations of egg yolk as a natural extracellular cryoprotectant was able to significantly influence (P<0.005) the quality of Lukas fish spermatozoa 48 hours after cryopreservation. The use of egg yolk concentration of 9% is the optimum concentration in maintaining the viability of Lukas fish spermatozoa with the average percentage obtained each being 80.75 ± 1.55%.
Assessment of potential cellular toxicity of methylglyoxal on primary human epidermal keratinocytes Prakoso, Nurul Muhammad; Sundari, Ayu Mulia; Fadhilah; Abinawanto; Dwiranti, Astari; Bowolaksono, Anom
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/JYICDMXO

Abstract

Skin is the outermost layer of the human body, which is important to prevent damage by infection, ultraviolet radiation, and toxic materials. The impermeable barrier of the skin has to be maintained via skin regeneration to preserve its essential function. However, skin regeneration declines due to decreased cell proliferation during skin aging. In this study, we investigated the role of methylglyoxal (MGO) as a precursor of advanced glycation end-products (AGE), which have been reported as an emerging threat of early aging in the skin. Previous studies reported a reduction in survival of the HaCaT cell line exposed to 500 µM MGO. However, studies on primary keratinocytes are still limited. We evaluated the viability and proliferation of primary human epidermal keratinocytes (HEKa) under the exposure of MGO. We also employed H2DCFDA assay to quantify the generation of intracellular ROS due to MGO. Our study revealed a biphasic response in which lower doses of MGO (25 and 50 µM) increased viability, while the cellular toxicity began at higher concentrations of 100, 200, and 300 µM. The proliferation assay also suggests an overall reduction of primary epidermal keratinocytes exposed to 100 and 200 µM MGO after 24 and 48 hours of culture. Increasing ROS concentrations were also observed in cells treated with 100 and 200 µM MGO. Our data suggested that a lower concentration of MGO was required to induce cellular toxicity in primary human epidermal keratinocytes compared to the HaCaT cell line reported by another study, indicating different sensitivity to MGO.
Pengaruh Variasi Konsentrasi Kuning Telur terhadap Abnormalitas Sperma Puntius bramoides Pascakriopreservasi 48 Jam Pertiwi, Primasari; Abinawanto
OKTAL : Jurnal Ilmu Komputer dan Sains Vol 4 No 06 (2025): OKTAL : Jurnal Ilmu Komputer Dan Sains
Publisher : CV. Multi Kreasi Media

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Abstract

The study aims to determine the effect of several concentrations of egg yolk as an effective natural cryoprotectant for cryopreservation and to analyze the quality of spermatozoa of Puntius bramoides fish (lukas fish) 48 hours after cryopreservation. This study used a Completely Randomized Design (CRD) with 9 treatments and 4 replications. The concentrations of egg yolk used as cryoprotectants were 0% (negative control), 5%, 7%, 9%, 11%, 13%, 15%, 17%, and the use of Carboxymethyl Cellulose (CMC) 1% as a positive control. The results showed that the use of several concentrations of egg yolk as a natural extracellular cryoprotectant was able to significantly affect (P <0.005) the abnormalities of spermatozoa of lukas fish 48 hours after cryopreservation. The use of 9% egg yolk concentration is the optimum concentration in reducing the percentage of abnormality of sperm in lukas fish with an average percentage obtained of 26.13 ± 1.49%.
Efek Kuning Telur Terhadap Motilitas Sperma Ikan Lukas (Puntius bramoides Val) Pascakriopreservasi Pertiwi, Primasari; Abinawanto; Farisi, Salman
BULLET : Jurnal Multidisiplin Ilmu Vol. 3 No. 1 (2024): BULLET : Jurnal Multidisiplin Ilmu
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research aims to determine the effect of several concentrations of egg yolk as an effective natural cryoprotectant for cryopreservation and to analyze the quality of lukas fish spermatozoa (Puntius bramoides Val) 48 hours after cryopreservation. This research used a 9 treatments and 4 replications. The concentration of egg yolk used as a cryoprotectant was 0% (negative control), 5%, 7%, 9%, 11%, 13%, 15%, 17%, and 1% Carboxymethyl Cellulose (CMC) was used as a positive control. The results showed that the use of several concentrations of egg yolk as a natural extracellular cryoprotectant was able to significantly influence (P<0.005) the quality of Lukas fish spermatozoa 48 hours after cryopreservation. The use of egg yolk concentration of 9% is the optimum concentration in maintaining the motility of Lukas fish spermatozoa with the average percentage obtained each being 80.45 ± 0.93%.
A Comparative Study of Penicillin G Acylase Expression in Two Escherichia coli Strains: BL21 (DE3) and Arctic Express Cendana, Kartika Sari; Wulandari, Sri Rezeki; Sabbathini, Gabriela Christy; Ulfah, Maria; Achnafani, Dini; Abinawanto; Aniqah, Sunni Sofiah; Helianti, Is; Nurhayati, Niknik
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.596-604

Abstract

The growing demand for semisynthetic beta-lactams has directed attention towards enzymes, specifically Penicillin G Acylases (PGAs), for their potential in synthesizing these antibiotics. This study delves into the expression of Achromobacter xylosoxidans PGA (AxPGA) in Escherichia coli, with a focus on enhancing the yield of active PGA, often constrained by a complex maturation process. The optimization of PGA expression included variations in IPTG concentration and the addition of CaCl2. Furthermore, the study compared PGA expression in E. coli BL21 (DE3) with that in E. coli Arctic Express (DE3), capable of co-expressing chaperones (chaperonin Cpn60 and Cpn10). Induction with 0.5 mM IPTG resulted in the highest hydrolytic activity in both strains, with Arctic Express (AE) exhibiting significantly higher activity due to improved folding facilitated by cold-adapted chaperonins. Alongside optimal IPTG induction, the addition of 10 mM CaCl2 in the culture media significantly increased PGA activity in both strains, highlighting that Ca2+ supplementation is an effective strategy to enhance the yield of functional PGA. Subcellular fractionation demonstrates that the periplasmic fraction yielded higher volumetric and specific activities compared to the cytoplasmic fractions in both E. coli strains, highlighting the importance of periplasmic processing for PGA maturation. This suggests that extracting the periplasmic fraction is an effective strategy for recovering active PGA while avoiding or reducing contamination either from co-expressed cytoplasmic chaperones or other intracellular proteins. These findings emphasize that induction strategy, ionic stabilization, and host strain selection play synergistic roles in increasing active recombinant PGA expression.
Co-Authors Achnafani, Dini Aniqah, Sunni Sofiah ANOM BOWOLAKSONO Arief Boediono Cendana, Kartika Sari Dwiranti, Astari Fadhilah Farisi, Salman Funahashi, Hiroaki IS HELIANTI Maria Ulfah NIKNIK NURHAYATI Prakoso, Nurul Muhammad Primasari Pertiwi Sabbathini, Gabriela Christy Sundari, Ayu Mulia Wulandari, Sri Rezeki Wulandari, Tri Aprilliana