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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Mutiara Medika: Jurnal Kedokteran dan Kesehatan Caraka : Indonesia Journal of Communication Kalimatuna: Journal Of Arabic Research
Tsabitah, Khansa
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Agriculture, Biological Sciences & Forestry Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology

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Analisis Wacana Kritis Pidato Pertama Kaesang Pangarep sebagai Ketua Umum Partai Solidaritas Indonesia Tsabitah, Khansa; Suryawati, Indah
CARAKA : Indonesia Journal of Communication Vol 5, No 1 (2024): Caraka : Indonesia Journal of Communication
Publisher : Indonesian Scientific Journal (Jurnal Ilmiah Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25008/caraka.v5i1.109

Abstract

Partai Solidaritas Indonesia mendeklarasikan Kaesang Pangarep sebagai Ketua Umum yang baru, dalam acara Kopdarnas: Deklarasi sikap politik PSI yang diselenggarakan pada 25 September 2023. Di kesempatan tersebut Kaesang Pangarep menyampaikan pidato pertamanya serta alasan terjun ke dunia politik. Banyak pro dan kontra di masyarakat karena begitu cepat pengangkatannya sebagai ketua umum, juga belum adanya pengalaman terkait politik. Penunjukkan Kaesang sebagai Ketua Umum PSI diartikan fokus kepada kekusaan saja. Jenis penelitian ini kualitatif dengan analisis wacana kritis model Teun A. Van Dijk. Penelitian ini bertujuan mengkaji struktur mikro, struktur supra, dan struktur makro pada teks pidato pertama Kaesang Pangarep sebagai ketua umum Partai Solidaritas Indonesia. Disimpulkan bahwa pidato yang disampaikan ada struktur wacana serta elemen pada pidato Kaesang Pangarep. Di setiap struktur wacana yang disampaikan terdapat ideologi yang diperlihatkan seperti ideologi liberalisme, ideologi nasionalisme, dan ideologi sosialisme. 
The Cytotoxicity of Agaro-Oligosaccharides and Neoagaro-Oligosaccharides on Macrophage Cells Pratitis, Visi Endah; Puspitasari, Pinki Anggrahini; Hanbal, Mayland Muhammad; Tsabitah, Khansa; Juliadmi, Dian; Saksono, Budi; Wijayanti, Nastiti
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 24, No 2 (2024): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v24i2.21059

Abstract

Agarose is a polysaccharide from red algae. Enzymatic hydrolysis of agarose can produce Agaro-Oligosaccharidess (AOS) and Neoagaro-Oligosaccharidess (NAOS). Different times of enzymatic hydrolysis can produce different types of AOS and NAOS based on their degree of polymerization (DPs). This study aims to examine the cytotoxicity of AOS and NAOS with different hydrolysis times on the macrophage cell line RAW 264.7. The parameters used were the percentage of cell viability and IC50 value. The cytotoxicity test using MTT assay and One-way ANOVA were used as statistical tests. The results showed that AOS-0 at a concentration below 125 µg/mL was not toxic and showed moderate toxicity up to a high concentration of 1000 µg/mL, while AOS hydrolyzed for 24 h (AOS-24) was not toxic to RAW 264.7 cells at all concentrations tested. The different results were shown in all NAOS samples, which were highly toxic to RAW 264.7 cells in the 125 to 1000 µg/mL, indicating that it was concentration dependent. The results showed that different hydrolysis times caused differences in the structure of AOS and NAOS compounds and influenced the toxicity level. Research development for further studies on antioxidants and anti-inflammatory needs more attention to the sample type and hydrolysis time.
Kualitas Buku Teks Bahasa Arab MTs Kelas VII: Studi Komparatif Buku Terbitan Kemenag RI dan Dar al-Nile Roja, Marwah Raisa; Tsabitah, Khansa
Kalimatuna: Journal of Arabic Research Vol 3, No 2 (2024): Kalimatuna: Journal of Arabic Research
Publisher : Department of Arabic Education - Faculty of Educational Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kjar.v3i2.43644

Abstract

This study examines the quality of Class VII Arabic textbooks published by Dar Al-Nile and the Ministry of Religion (Kemenag), based on W.F. Mackey's theory, which encompasses elements of selection, gradation, presentation, and repetition. The book published by the Ministry of Religion aims to support the national curriculum by integrating moderate Islamic values with a context-based thematic approach. In contrast, Dar Al-Nile’s textbook is noteworthy for its application of communicative and phonetic methods, emphasizing speaking skills in a more systematic manner. The researcher employed a descriptive qualitative method, utilizing primary data sources from the Arabic textbooks for MTs Class VII published by the Ministry of Religion of the Republic of Indonesia and Dar Al-Nile. The analysis reveals that the Ministry of Religion’s book excels in the selection of diverse vocabulary that aligns with local needs, while Dar Al-Nile’s textbook is distinguished by its effective material gradation and appealing visual presentation. To enhance contextual and tailored learning experiences for students in Indonesia, this study recommends the development of Arabic textbooks that integrate the strengths of both approaches.
In-Silico Optimization of α-agarase for Sustainable Bioprospecting of Agaro-oligosaccharides: In-Silico Optimization of α-agarase for Sustainable Bioprospecting of Agaro-oligosaccharides Hanbal, Mayland Muhammad; Wijayanti, Nastiti; Tsabitah, Khansa; Zulfa, Amania; Tasania, Nadia Nisa; Saksono, Budi
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/gg6mkk56

Abstract

Enzymatic synthesis is a sustainable alternative to chemical methods, offering high substrate specificity, reduced byproducts, and environmentally friendly processes. Despite its advantages, agaro-oligosaccharides (AOS) production largely depends on chemical synthesis due to the limited exploration of α-agarase. Therefore, this study aims to identify, analyze, and redesign a suitable α-agarase candidate for AOS production using in-silico approaches. Biological databases from CAZy, UniProtKB, and PDB, along with journal references, were used to curate α-agarase candidates. Non-catalytic regions were removed to retain only the GH96 catalytic domain, with a His-tag added for easier purification. Subsequently, structural modeling using SWISS-MODEL was performed to facilitate blind docking with CB-Dock2. Modeling also facilitated physicochemical properties predictions incorporating OphPred, Protein-Sol, and SCooP for pH, solubility, and thermal stability. The results showed that AgaA33, obtained from Thalassomonas agarivorans JAMB-A33, was selected due to its high annotation score and optimal temperature. Structural modeling and blind docking confirmed that the functional domains were preserved after redesign. In-silico physicochemical assessments revealed that the redesigned enzyme exhibited improved pH tolerance and thermal stability, despite a slight reduction in solubility. This study showed the use of computational tools for enzyme redesign and showed the potential of α-agarase as a green and sustainable biocatalyst for AOS production. By combining database-driven candidate selection with in-silico structural and functional analyses, these results set the foundation for further optimization of α-agarase to meet industrial needs. Future efforts must focus on improving solubility and refining activity predictions to fully realize the enzyme’s potential for eco-friendly bioprocesses.
Co-Authors BUDI SAKSONO Hanbal, Mayland Muhammad Indah Suryawati Juliadmi, Dian Nastiti Wijayanti Pratitis, Visi Endah Puspitasari, Pinki Anggrahini Roja, Marwah Raisa Tasania, Nadia Nisa Zulfa, Amania