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All Journal Veterinary Biomedical and Clinical Journal
Lastuti , Nunuk Dyah Retno
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

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The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method: Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing Kismawardani, Putri; Koesdarto , Setiawan; Santoso , Kuncoro Puguh; Lastuti , Nunuk Dyah Retno; Sabdoningrum , Emy Koestanti; Kusnoto
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.2

Abstract

This study aimed to identify the parasitic nematode Hysterothylacium sp. using the sequencing method. A sampling of Hysterothylacium sp. was taken from 60 striped grouper fish (Ephinephelus sexfasciatus) and has characteristics such as brown color, booring teeth on the anterior, a simple digestive system including the esophagus, stomach (ventriculus), small intestine and does not have mucron on the posterior. Specific primers for the COX-2 mtDNA gene (210F and 211R) were used for DNA amplification (Polymerase Chain Reaction) and DNA sequencing with a length of 629bp. The positive result of the PCR method (sample S) was sequenced. Then, the results of the sequencing method were compared with the reference DNA sequences KY065298.1, MF120253.1, MF120248.1, and KU886687.1. Furthermore, Homology and kinship tests were carried out using GenBank (NCBI). Results showed a 97% similarity of the sample with Hysterothylacium amoyense cytochrome oxidase subunit II (COX-2) gene MF120253.1.
Identification and Prevalence of Endoparasites in Timor Deer (Cervustimorensis) at Maliran Deer Captive Breeding Blitar Khairunnisa, Anindita; Lastuti , Nunuk Dyah Retno; Sugihartuti , Rahmi; Setiawan, Boedi; Koesdarto , Setiawan; Wardhana, Dhandy Koesoemo
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.6

Abstract

Deer populations in captive breedings have the potential to develop health issues such as protozoa and helminth infections. Poor sanitation and environmental conditions are among the external factors facilitate the transmission of endoparasites within deer population in a captivity. The aim of this study was to identify and determine the prevalence of endoparasite infestation in Timor deer (Cervus timorensis) in Maliran Captive Breeding, Blitar, Indonesia. Samples of 102 fresh feces, were taken during the rainy season from March to May 2021, and sent to Laboratory of Parasitology, Veterinary Medicine Faculty, Universitas Airlangga for fecal examination using sedimentation, flotation, as well as McMaster methods to determine the prevalence and the intensity of infestations. Results showed that 30 samples were detected positive, 7 samples (6.86%) were positive of Haemonchus spp. at the egg stage (range of 30-60 EPG; mild infestation); and 23 samples (22.55%) were positive of Eimeria spp. in oocyst stage (range of 200-800 OPG; mild to moderate infestation). This study provides a reference for the eradication of Haemonchiasis in the deer population in the study area.
Identification and The Prevalence of Gastrointestinal Endoparasite in Long-Tailed Macaque (Macaca Fascicularis) in Wonorejo and Gunung Anyar Mangrove Eco-Tourism Surabaya Fauziyah , Nafisah Nurti; Lastuti , Nunuk Dyah Retno; Damayanti , Ratna; Koesdarto , Setiawan; Suprihati , Endang; Witaningrum, Adiana Mutamsari
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.5

Abstract

This study aimed to determine the types of endoparasites and their prevalence in Macaca fascicularis in the Wonorejo and Gunung Anyar mangrove forests, Surabaya. A total of 90 samples of Macaca fascicularis faeces were collected from two different mangrove forests, 45 samples from Wonorejo and 45 from Gunung Anyar. All samples were examined using sedimentation and floating methods to identify endoparasite species. Further, the number of eggs and oocysts per gram of feces was counted using the McMaster method. Results showed that 26.67% (12/45), and 22.2% (10/45) of the faecal samples were detected positive for endoparasite at Wonorejo and Gunung Anyar mangrove forests, respectively. The prevalence of parasitosis in M. fascicularis from Wonorejo was 17.7% (8/45) for Taenia sp. and 8.8% (4/45) for Balantidium coli cyst. On the other hand, M. fascicularis from Gunung Anyar was infested by Trichuris sp. at a prevalence of 22.2% (10/45). The total number of eggs and cysts ranged from 30 – 400 per gram of faecal samples. The study provides new knowledge on the parasitic burdens among long-tailed macaques in East Java.
Co-Authors Adiana Mutamsari Witaningrum Boedi Setiawan Damayanti , Ratna Fauziyah , Nafisah Nurti Khairunnisa, Anindita Kismawardani, Putri Koesdarto , Setiawan Kusnoto Sabdoningrum , Emy Koestanti Santoso , Kuncoro Puguh Sugihartuti , Rahmi Suprihati , Endang Wardhana, Dhandy Koesoemo