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All Journal Buletin Veteriner Udayana Jurnal Veteriner Majalah Kedokteran Bandung Media Kedokteran Hewan Majalah Obstetri dan Ginekologi Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences.

Boedi Setiawan

Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia

Author-ID : 376810

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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PENGARUH TRANSPLANTASI ALLOGRAF PANCREATIC STEM CELL TERHADAP KADAR INSULIN DAN C-PEPTIDE TIKUS PUTIH PENDERITA DIABETES MELITUS TIPE I Setiawan, Boedi; Plumeriastuti, Hani
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Penyakit diabetes melitus merupakan salah satu penyakit degeneratif yang hingga kini masih belum tuntas terapinya dan masih menjadi ancaman serius bagi dunia kesehatan di Indonesia dan dunia. Tujuan penelitian ini adalah mengetahui kadar insulin dan C-peptide tikus putih penderita diabetes melitus tipe I yang diberikan transplantasi allograf pancreatic stem cell dengan laparotomi intrapankreatik. Penelitian ini dilakukan selama 6 bulan (Juli?Desember 2014) di lab. Stem Cell, Institute of Tropical Diseases, Universitas Airlangga, Surabaya. Dua belas tikus putih jantan Rattus novergicus galur Wistar dibagi secara acak menjadi dua kelompok . Kelompok pertama (P0) disuntik aloksan 150 mg/kg bobot badan tanpa terapi stem cell . Kelompok kedua disuntik aloksan dengan dosis 150 mg/kg bobot badan dan diterapi dengan 1x106/kg bobot badan stem cell pankreas secara laparotomi intrapankreatik (P1). Akhir penelitian adalah pada hari ke-31 percobaan. Hasil penelitian menunjukkan bahwa kadar glukosa darah pada akhir penelitian berbeda sangat nyata (p <0,01) antara kelompok perlakuan yang menerima terapi stem cell (P1) dengan P0 kontrol positif, meskipun nilai kadar glukosa darah rata-rata tidak senormal seperti pada hari ke-1. Tingkat C-peptide dan insulin P0 dan P1 berbeda sangat nyata (p<0,01). Dapat disimpulkan bahwa terapi stem cell secara laparotomi intrapankreatik dapat menurunkan kadar glukosa darah, serta meningkatkan kadar C-peptide dan insulin. [MKB. 2016;48(3):135?39]Kata kunci: Diabetes melitus, insulin, stem cell Insulin and C-peptide Levels in Diabetes Mellitus Type I White Rats treated with Pancreatic Stem Cell Allograft TransplantationDiabetes mellitus is one of the degenerative diseases in which the therapy still remains unresolved and is still a serious threat to the global health, including to the health of Indonesian people. The aim of this study was to describe the level of insulin and C-peptide in diabetes mellitus type I white rats treated with pancreatic stem cell allograft through intrapancreatic laparotomy. This study was conducted at the Institute of Tropical Diseases, Universitas Airlangga, Surabaya in a 6 month period (July?December 2014). Twelve male white rats Rattus novergicus Wistar strain, were randomly divided into two groups. The first group (P0) was injected by alloxan, 150 mg/kg body weight, without stem cell therapy. Another group was injected by alloxan, 150 mg/kg body weight, and was treated with 1x106/kg body weight pancreatic stem cell throughintrapancreatic laparotomy (P1). The experiment was finalized on the 31th day of the experiment. The results showed that the blood glucose levels at the end of experiment were highly significantly different p<0.01 between the treatment group that received stem cell therapy (P1) and P0 positive control, although the average value of blood glucose levels was not as normal as on the first day. C-peptide and insulin levels of P0 and P1 group differed significantly (p<0.01). It can be concluded that stem cell therapy through intrapancreatic laparotomy can reduce blood glucose levels and increase the levels of C-peptide and insulin. [MKB. 2016;48(3):135?39]Key words: Diabetes mellitus, insulin, stem cell

Terapi Fotodinamik Mempercepat Kesembuhan Luka Insisi pada Kulit Tikus Putih (Rattus novergicus) yang Diinfeksi Bakteri Methicilin-Resistant Staphylococcus aureus Yonatan Dimascahyo Budianto; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Hani Plumeriastuti; Wiwiek Tyasningsih; Boedi Setiawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.591 KB)

The aims of this research was to determine the effect of photodynamic therapy (PDT) on the healing of incision wounds in rat skin (Rattus norvegicus) that infected with MRSA bacteria by evaluating and give a score (0 – 4) at wound healing factors such as epithelialization, angiogenesis, inflammatory cells, fibroblast cells and collagen density. This study used 25 white rats divided randomly into 5 groups with 5 replications, namely P0 = MRSA infection wound + Povidone Iodine, P1 = MRSA infection wound + Silver Sulfadiazine, P2 = MRSA infection wound + 5-minute PDT irradiation, P3 = MRSA infection wound + 10- minute PDT irradiation and P4 = MRSA infection wound + 15-minute PDT irradiation. Histopathological observations and scoring showed epithelialization and collagen density in groups P2, P3 and P4 increased significantly compared to groups P0 and P1 (p<0.05). The histopathological observations and scoring of angiogenesis, inflammatory cells, fibroblast cells of P0 and P1 groups had a higher score than the treatment group with photodynamic therapy P2, P3 and P4 (p<0.05). This condition showed that in the P0 and P1 groups are still in the inflammatory phase, while in the P2, P3 and P4 groups have entered the remodeling phase which is characterized by high epithelialization scores and collagen density. Giving photodynamic therapy to wounds infected with MRSA can accelerate the wound healing process.

Perbandingan Kadar Elektrolit Serum Pascaenterektomi Ekstensif 75 % pada Anjing yang Diterapi dengan Laktoferin Boedi Setiawan; Sudarminto -; Hartiningsih -
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.342 KB)

A severe and extensive intestine damages can occur in dogs suffering from volvulus , strangulation,intersuception and neoplasia. Extensive enterectomy is the most common medical treatment for dogssuffering from such disorder. A study was therefore conducted to compare the serum electrolyte level ofextensively enterectomyzed dogs after being treated with different level (0%, 0.05% and 0.5 mg/bodyweight) of lactoferin. As many as 9 dogs at 3-4 moth-old and with the body weights ranging from 4-5 kgwere used. After being enterectomized to the extent of 75% out of the total length of the intestine, the dogswere divided randomly into 3 groups (I, II and III) each of which consisted of 3 dogs. The three groups weretreated for 30 days respectively with 0.0 mg/body weight, 0.05mg/kg body weigth and 0.5mg/kg bodyweight of lactoferin. The electrolyte levels (Na, K and Cl ion) of dogs were determined at days 1, 15 and 30of during the treatment. The data collected from this study were analysed by Analysys of Variance (Anova)proceded by Duncan Multiple Range test (DMRT). The result showed that at days 15 and 30 of thetreatment, the electrolyte levels of the dogs with lactoferin (0.05 and 0.5 mg/body weight) were significantlyhigher than in dogs without lactoferin (0mg/bodyweight). The level of Na ion of dogs with 0,5, 0,05, dan 0mg/kg bw lactoferin were 143, 143,4, dan 141,7 mEq/L respectively at day 15 and 147, 150, dan 137,7mEq/L respectively at day 30. The levels of K ion for those dogs were 5,17, 4,97, dan 3,83 mEq/Lrespectively at day 15 and 30: 5,1, 5,13, 3,73 mEq/L respectively at day 30. Meanwhile, their Cl levelswere 113,7, 114,3, 104 mEq/L respectively at day 15 and 115,3, 117,3, dan 91,3 mEq/L respectively atday 30. Hasil penelitian ini menunjukkan bahwa memperian lactoferin memperbaiki kadar elektrolitserum anjing yang dienterektomi secara ekstensif. It was evidence that lactoferin treatment can improvedthe electrolyte profiles of extensively enterotomized dogs.

New vesicovaginal fistula formation in a rabbit model Eighty M Kurniawati; Tri HS Hadi; Nur AR Widiatmoko; Widjiati Widjiati; Fedik A Rantam; Budi Santoso; Budi I Santoso; Hari Paraton; Gatut Hardianto; Azami D Azinar; Boedi Setiawan
Majalah Obstetri dan Ginekologi Vol. 27 No. 3 (2019): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V27I32019.114-118

Objective: To create an animal model of vesicovaginal fistula as a modality to develop therapy.Material and Methods: This study was an preliminary experimental study to create new fistula vesicovaginal formation in a rabbit model. Six female New Zealand white rabbits of Stem Cell Research and Development Center of Universitas Airlangga were used in this study and were divided into 3 groups in which there were 2 rabbits in each group. Vesicovaginal fistula formation was performed through laparotomy surgery. After the abdomen is opened, the Nasogastric tube is inserted vaginally into the anterior vaginal wall. The incision is made on the anterior vaginal wall and the posterior wall of the bladder. The Nasogastric tube that comes out of the vagina is inserted into the bladder incision to maintain the formation of vesicovaginal fistula. Both mucosal walls are sutured and tested to determine if there is any leak. Evaluation the model of vesicovaginal fistula was carried out 3 weeks postoperatively according the group.Result: Four out of six rabbits have formed vesicovaginal fistulas, which are from groups two and three. There are no complications during the treatment period until terminationConclusion: Rabbits have the potential to become experimental animals for the vesicovaginal fistula model. The best time to release a nasogastric tube to maintain a fistula is 2 weeks.

Prevalence and Intensity of Nematode Infection on The Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java Sesa Puput Febriyanti; Lucia Tri Suwanti; Eka Pramyrtha Hestinah; Setiawan Koesdarto; Boedi Setiawan; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This survey study was aimed to determine the prevalance of nematodosis and intensity on the Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java. The benefits of this research are to contribute giving additional information about Prevalence, Intensity, and Relation among prevalance with age, sex, and stable. Fecal samples were tested by native, sedimentation, and floatation methods. The examination was found some kind of worm eggs, which are: Trychonema sp., Strongylus spp., Dictyocaulus arnfieldi (lung worm). The result showed that the prevalence of nematodosis was 12.03%. in the calculation of worm eggs per gram feces of Crossbreed Horse in Detasemen Kaveleri Berkuda that have relatively moderate infection were 1123EPG and result analyze with ANOVA showed no real different with function of horse. The result of statistical analysis using Chi-Square test showed no significant differences in prevalence with age and sex (p>0.05). the result of statistical using Chi-Square test showed significant differences in prevalence with stable in Detasemen Kaveleri Berkuda (p<0.05).

The Effect of Folic Acid as Supportive Therapy of Spiramycine on Weight of Foetus to Toxoplasma gondii – Infected Pregnant Mice (Mus musculus) Alfina Azkiana; Boedi Setiawan; Erma Safitri; Lucia Tri Suwanti; Mufasirin Mufasirin; Djoko Legowo
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aimed to investigate the effect of folic acid as supportive therapy of spiramycine on weight of foetus to Toxoplasma gondii-infected pregnant mice (Mus musculus). Twenty pregnant female mice were divided into four groups as C -, C +, T1 and T2. C +, T1 and T2 were infected by Toxoplasma gondii. C – and C + administered orally 0.5 ml aquadest, T1 administered orally 130 mg/kg BW spiramycine and 0.052 µg/g BW folic acid and T2 administered orally 0.052 µg/g BW folic acid. Experimental groups received the treatments for 5 days, then animals of each groups were sacrified. Foetuses were dissected out for observation. The weight of fetuses were measured using an analytical balance. The data weight of foetuses was presented descriptively and analyzed by ANOVA test and continued by Tukey HSD. From this study, the weight of foetuses from the pregnant mice of T1 and T2 have difference compared with the controls. The result of this research is folic acid affects the weight of foetuses to Toxoplasma gondii-infected pregnant mice.

The Distribution of Goat Gastrointestinal Tractus Worm Egg at Rambon District of Nganjuk Regency Arum Puspitasari; Boedi Setiawan; Setiawan Koesdarto; Kusnoto Kusnoto; Soeharsono Soeharsono; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the distribution of worm tract infection in foat digestion in Sub-district of Prambon, Nganjuk Regency. This research was conducted in Agustus 2018-Januari 2019 with 108 samples of stool examination iin laboratory of Helmintologi Airlangga University Department of Parasitology, were examinated by native, sedimentation. Floatation techniques, and distribution of worm types of feces. The results showed that 66 samples were positive infected by gastrointestinal worm, its indicated that helminthiasis was 61,11%. On examination it was found some kind of worm eggs, which are: Oesophagustomum spp., Bunostomum spp., Haemonchus spp., Trichostrongylus spp., Strongyloides spp., Trichuris spp., Chabertia spp and Moniezia benedini. The results of study showed the most of worm distribution in feces is Haemonchus spp.

Identifikasi Protozoa pada Darah dan Saluran Pencernaan Biawak Air (Varanus salvator) Azizah Bilqis Nurkarimah; Mufasirin Mufasirin; Ratna Damayanti; Lucia Tri Suwanti; Boedi Setiawan; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research is to identify the variaous of protozoa in the bloodanddigestive tract on water monitor lizard (Varanus salvator) was captured in Sidoarjo, East Java. This research was taken on March until June 2019. This research used a non-experimental method through an observation study. As many as 50 water monitor lizard were used this research and examined at the Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga.The samples of this research were made in thin blood smear using Giemsa stain, while fecal examination using native method, sucrose flotation, and modified Ziehl-Neelsen stain.The results showed that two various of protozoa found were single infection. Observations on the blood was obtained Haemogregarina sp. (14%) and observations of feces obtained Eimeria sp. (2%). The conclusion of this research indicate that type of protozoa was Haemogregarina sp. and Eimeria sp. were found on water monitor lizard(Varanus salvator).

Identifikasi dan Pola Infestasi Caplak pada Biawak Air (Varanus salvator, Byers, d. 2000) Kartika Aditiya Amelia; Rahaju Ernawati; Poedji Hastutiek; Boedi Setiawan; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study is to identify the type of ticks infestation pattern on water monitor lizard (Varanus salvator, Byers, D. 2000) with regard parameters water monitor lizard species, stadium and region of the water monitor lizardâ€™s body. The total samples of 250 ticks from 30 water monitor lizard (18 male lizards and 12 female lizards) were collected every day starting from noon to evening during July to August 2019 at the monitor slaughtering place in Buduran District, Sidoarjo Regency. The tick samples were identified by the permanent mounting slide method without staining and put in bottles containing 10% KOH for 1-10 hours, then each stadium used a nikon eclipse E100 microscope with a 40x magnification binocular microscope. Data was analyzed using Multiple Correspondance Analytics. The result indicate that 30 V. salvator (100%) are positive infested by tick and the samples obtained consisted of 133 Aponomma sp. and 117 Amblyomma sp. in all regions of the body.

Identification of Acanthocephala on Water Monitor Lizard (Varanus salvator) Human Consumed in Sidoarjo Ahmad Herdianto Wicaksono; Emmanuel Djoko Poetranto; Tatik Hernawati; Boedi Setiawan
Journal of Parasite Science (JoPS) Vol. 4 No. 2 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Water monitor lizards are consumed by humans for its meat. The demand this reptile consumption because the taste and belief of the people in healing disease. Based on habitat and food, they have the potential to transmit zoonosis and have a high possibility of being infested by parasites. One of the possible disease that could infect water monitor and transmitted to human is acanthocephaliasis, infection from Acanthocephalan. Which previous research found Acanthocephala on intestine of frog. This research aims to know and identify Acanthochepala worms that infect water monitor lizards. Based on the results, 40 samples were used and there are 2 positive samples with Acanthocephalan in the body cavity.

Co-Authors A'la, Rofiqul Abdul Samik, Abdul Agus Wijaya Agustono, Bodhi Ahmad Herdianto Wicaksono Aldin Akbar Rahmatullah Alfina Azkiana Ana Rohmatul Laili Aprinda Ratna Lovela Arimbi Arum Puspitasari Aswin, Ahmad Azami D Azinar Azizah Bilqis Nurkarimah Bambang Poernomo Bambang Sektiari Lukiswanto Budi I Santoso Budi Santoso Budi Utomo Dadik Rahardjo, Dadik Daniel Leonardo Darmawan, Moch. Rizky Dellavia, Yolanda Diyantoro Diyantoro, Diyantoro Djoko Legowo Djoko Legowo Doohan Mahendra Eduardus Bimo Aksono Edward Yonas Kristijanto Eighty M Kurniawati Eka Pramyrtha Hestianah, Eka Pramyrtha Eka Pramyrtha Hestinah Emmanuel Djoko Poetranto Endang Suprihati Endang Suprihati Epy Muhammad Luqman Erma Safitri Faisal Fikri Faizah, Ayu Nur Febriyatin, Fierdausi Nur’Aini Fedik A Rantam Fedik Abdul Rantam Fianti Nufinda Rachma Gatut Hardianto, Gatut Ghifari Lutfi Fauzi Hani Plumeriastuti Hari Paraton Hartiningsih - Helen Susilowati, Helen Hendarti, Gracia Angelina Heni Puspitasari Herupradoto, Eduardus Bimo Aksono Hidajati , Nove Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Indasari, Elly Nur Indriyanti, Astrid Ira Sari Yudaniayanti Ismudiono Ismudiono Ismudiono, Ismudiono Istiana, Izzatul Iwan Sahrial Hamid Jola Rahmahani Kadek Rachmawati Kadek Rachmawati Kamilah Nurizza Kartika Aditiya Amelia Khairunnisa, Anindita Koesdarto , Setiawan Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lastuti , Nunuk Dyah Retno Legowo, Djoko Lestari, Dewi Sri Lilik Maslachah LINA SUSANTI Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Melda Mufidatul Ifqiyyah Mochamad Lazuardi Mohamad Safri Sauqi Mufasirin Mustofa Helmi Effendi Nidom, Chairul Anwar Niken Hastuti, Apriliwiyani Nove Hidajati Nur AR Widiatmoko Nusdianto Triakoso Pinayungan, Probo Probo Warih Tatag Poedji Hastutiek Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Pratama, Ponasari Galuh Primarizky, Hardany Pudji Srianto Puput Ade Wahyuningtyas Purbowati, Tri Endah Rachmanita, Rona Taufiqul Rahaju Ernawati Rahmahani, Jola Rahmandari, Dina Agylia Ramadhanty, Miladhiyah Nabila Ratna Damayanti Ratna Damayanti Retno Wulansari Rochmah Kurnijasanti Saputro, Amung Logam Sari, Fifin Kurnia Sesa Puput Febriyanti Setiawan Koesdarto Soeharsono Soeharsono Soeharsono Soeharsono Sofyan, Miyayu Soneta Sri Hidanah Sri Mulyati Srimaryanto, Leonardo Reza Sudarminto - Sudjono, Budi Santoso Sugihartuti , Rahmi Sunarso, Agus Syahraini Adhiya Lathifah Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri HS Hadi Tri Lestari Tri Wahyu Suprayogi Triagil, Annisa Lita Triakoso , Nusdianto Tyasningsih, Wiwiek Tyasningsih, Wiwiek Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati Wijaya, Andi Yasmin Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Yeni Dhamayanti Yeni Dhamayanti Yonatan Dimascahyo Budianto Yudhana, Aditya Yulianto, Rangga

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