Wardhana, Dhandy Koesoemo
Division Of Veterinary Public Health, Faculty Of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia

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PENINGKATAN SUMBER DAYA PANGAN ASAL HEWAN DI KECAMATAN KANDANGAN KABUPATEN KEDIRI Adiana Mutamsari Witaningrum; Dhandy Koesoemo Wardhana; Dian Ayu Permatasari
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 2 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i2.2020.243-250

Abstract

Food of animal origin is used for human consumption such as meat, milk, eggs and their derivatives. Food safety for food of animal origin is needed to prevent biological, chemical and physical contamination that can interfere of human health. One of the animal-based food businesses that are mostly managed in the Kandangan, Kediri is milk. Activities carried out in this community service program are counselling and demonstrations related to increasing food resources of animal origin in the area. The community was able to understand this extension activity on the importance of increasing food resources of animal origin and was able to participate in demonstrations of making yogurt. The results of the pre-test and post-test on public knowledge and awareness of the evaluation of the delivery that have been given show an increase from an average of 89.1 points to 100 points. This activity is able to increase the knowledge and skills of the local community on how to increase animal-based food resources in order to develop their business.Pangan asal hewan dimanfaatkan untuk konsumsi manusia yang berupa daging, susu, telur dan hasil turunannya. Keamanan pangan pada pangan asal hewan diperlukan untuk mencegah cemaran biologis, kimia dan fisik yang dapat mengganggu kesehatan manusia. Salah satu usaha pangan asal hewan yang banyak dikelola di daerah Kecamatan Kandangan, Kabupaten Kediri yaitu susu. Kegiatan yang dilaksanakan dalam program pengabdian masyarakat ini berupa penyuluhan dan demonstrasi terkait peningkatan sumber daya pangan asal hewan di Kecamatan, Kabupaten Kediri. Masyarakat mampu memahami kegiatan penyuluhan ini akan pentingnya peningkatan sumber daya pangan asal hewan dan mampu mengikuti demonstrasi pembuatan yoghurt. Hasil pre-test dan post-test pada pengetahuan dan kesadaran masyarakat akan evaluasi dari penyampaian yang telah diberikan menunjukkan peningkatan dari rata-rata mendapatkan 89.1 poin menjadi 100 poin. Kegiatan ini mampu meningkatkan pengetahuan dan keterampilan masyarakat sekitar bagaimana cara meningkatkan sumber daya pangan asal hewan dalam rangka mengembangkan usahanya.
Culling Layer Hen Ectoparasite Detection Sold at Wonokromo Market Surabaya City Nashihatus Sholihah; Sherbina Bai; Sri Hidanah; Dhandy Koesoemo Wardhana; Agus Sunarso; Poedji Hastutiek; M.Gandul Atik Yuliani
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.001 KB) | DOI: 10.20473/jops.v6i2.34077

Abstract

The purpose of the research were to identify the species, prevalence, and predilection of ectoparasite in Culling Layer Hen at Wonokromo Market, Surabaya City. The research was conducted from March to May 2021 with total of 100 sample of chickens were examined by the skin and feather body region. Identification of ectoparasite was carried out at The Laboratory Parasitology, Faculty of Veterinary Medicine Universitas Airlangga using permanent mounting methods. The results showed, the species of ectoparasite that infest the culling layer hen were Menopon gallinae 21%, Lipeurus caponis 10%, Ornythonissus bursa 10%, and Dubininia melopsitacci 70%. Predilection of ectoparasite shows that M. gallinae were found in the head-neck, back, wings, and legs region. L. caponis was found in the head-neck, wings, and back region. O. bursa was found in the legs and tail region, and D. melopsitacci was found in the entire body of chicken, the head-neck, back, wings, abdomen, legs, and tail.
PHYLOGENETIC ANALYSIS AND VIRULENCE-CODING GENES CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM LAYING HEN BRAIN WITH TETELO SYMPTOM Safira Iqlima Sarah; Christian Marco Hadi Nugroho; Ryan Septa Kurnia; Dhandy Koesoemo Wardhana; Heni Puspitasari
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.298 KB) | DOI: 10.21157/j.ked.hewan.v13i3.14493

Abstract

Newcastle disease (ND) is an acute poultry disease caused by Paramyxovirus group. It has characteristic neurological symptoms, called torticollis. The molecular assay to find out the presence of viral genes in the brain can be an option in detecting ND virus infections since it penetrates brain barrier system. The purpose of this study was to identify ND viruses in the brain of chickens with torticollis symptoms, to analyze its phylogenetic and to characterize its virulence genetic code. Samples used were 12 dead chickens with historically had torticollis symptoms, obtained from poultry farms at several areas in West Java and Banten. Chicken brains were prepared for reverse transcriptasepolymerase chain reaction (RT-PCR) test. All positive samples then sequenced to obtain its nucleotide sequences from some of Fusion (F) genes analyzed its phylogenetic by comparing with Indonesian ND isolate virus from GenBank using Mega X software. The results of RT-PCR test showed that only one sample (Virus MSL.03) contained genes of ND virus. Based on homology tests and phylogenetic analysis, the virus belonged to subgenotype VIIh with an identical level of 95.34-95.86% when compared to several isolates from Indonesia. The MSL.03 ND virus has 112RRRKRF117 pattern in F0 indicatest its virulent category.
Identification and Prevalence of Endoparasites in Timor Deer (Cervustimorensis) at Maliran Deer Captive Breeding Blitar Khairunnisa, Anindita; Lastuti , Nunuk Dyah Retno; Sugihartuti , Rahmi; Setiawan, Boedi; Koesdarto , Setiawan; Wardhana, Dhandy Koesoemo
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.6

Abstract

Deer populations in captive breedings have the potential to develop health issues such as protozoa and helminth infections. Poor sanitation and environmental conditions are among the external factors facilitate the transmission of endoparasites within deer population in a captivity. The aim of this study was to identify and determine the prevalence of endoparasite infestation in Timor deer (Cervus timorensis) in Maliran Captive Breeding, Blitar, Indonesia. Samples of 102 fresh feces, were taken during the rainy season from March to May 2021, and sent to Laboratory of Parasitology, Veterinary Medicine Faculty, Universitas Airlangga for fecal examination using sedimentation, flotation, as well as McMaster methods to determine the prevalence and the intensity of infestations. Results showed that 30 samples were detected positive, 7 samples (6.86%) were positive of Haemonchus spp. at the egg stage (range of 30-60 EPG; mild infestation); and 23 samples (22.55%) were positive of Eimeria spp. in oocyst stage (range of 200-800 OPG; mild to moderate infestation). This study provides a reference for the eradication of Haemonchiasis in the deer population in the study area.
Detection of Escherichia coli Contamination using Most Probable Number (MPN) methods in Chicken Meats in Market of Surabaya Wardhana, Dhandy Koesoemo; Safitri, Devi Ayu; Annisa, Suwaibatul; Effendi, Mustofa Helmi; Harijani, Nenny
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.118-124

Abstract

Foodborne diseases are diseases transmitted through contaminated food and are one of the main public health problems. The bacteria that usually contaminates chicken meat is Escherichia coli. E. coli contamination in chicken meat can affect consumer health. Chicken meat processing and market conditions affect the level of bacteria contamination. This study aims to measure the contamination of E. coli bacteria in chicken meat in Surabaya's markets. The method which used to count and estimate the number of E. coli bacteria found in chicken meat in this study was Most Probable Number (MPN). The results showed 20 samples (33.3%) from 60 samples gave negative results. This indicated that only 20 samples of chicken meat were safe for consumption because the MPN value is <1 í— 101 CFU/g while the remaining 40 samples (66.7%) were not safe for consumption because the MPN value is> 1 í— 101 CFU/g. The standard of SNI 3924: 2009 concerning the minimum requirement for the amount of E. coli contamination in chicken meat is 1í—101 CFU/g so that the contamination of E. coli which found in chicken meat in Surabaya was high.
Commercial Feed Substitution with Milkfish Waste Flour (Chanos chanos) Against Quail (Coturnix coturnix japonica) Egg White Index, Egg Yolk Index and Haugh Unit Annisa Nur Aulia Purwantoro; Sri Hidanah; Dhandy Koesoemo Wardhana; Mohammad Anam Al Arif; Sunaryo Hadi Warsito; Widya Paramita Lokapirnasari
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51497

Abstract

Quail farming is quite developed, from year to year the population increases. The search for alternative feed as a substitute for feed sources is an effort by breeders to minimize feed costs. One alternative to replace feed ingredients can be fishery industry waste. Fishery waste that can be used as feed is milkfish waste (Chanos chanos). The purpose of this research was to determined the substitution of milkfish waste flour (Chanos chanos) at commercial feed to egg white index, egg yolk index and haugh unit. There are 20 quails (Coturnix coturnix japonica) at one day of age of four treatments and five replications. Each treatment consisted by five females. The treatment consisted of P0 with commercial feed 100%, P1 with commercial feed 98% + milkfish waste flour 2%, P2 with commercial feed 96% + milkfish waste flour 4% and P3 with commercial feed 94% + milkfish waste flour 6%. Based on results of the Analysis of Variance (ANOVA) there was not significantly different (p>0,05) on egg white index, egg yolk index and haugh unit. It can be concluded the substitution of commercial feed with milkfish waste flour (Chanos chanos) can maintain egg white index egg yolk index and haugh unit.
PHYLOGENETIC ANALYSIS AND VIRULENCE-CODING GENES CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM LAYING HEN BRAIN WITH TETELO SYMPTOM Sarah, Safira Iqlima; Nugroho, Christian Marco Hadi; Kurnia, Ryan Septa; Wardhana, Dhandy Koesoemo; Puspitasari, Heni
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i3.14493

Abstract

Newcastle disease (ND) is an acute poultry disease caused by Paramyxovirus group. It has characteristic neurological symptoms, called torticollis. The molecular assay to find out the presence of viral genes in the brain can be an option in detecting ND virus infections since it penetrates brain barrier system. The purpose of this study was to identify ND viruses in the brain of chickens with torticollis symptoms, to analyze its phylogenetic and to characterize its virulence genetic code. Samples used were 12 dead chickens with historically had torticollis symptoms, obtained from poultry farms at several areas in West Java and Banten. Chicken brains were prepared for reverse transcriptasepolymerase chain reaction (RT-PCR) test. All positive samples then sequenced to obtain its nucleotide sequences from some of Fusion (F) genes analyzed its phylogenetic by comparing with Indonesian ND isolate virus from GenBank using Mega X software. The results of RT-PCR test showed that only one sample (Virus MSL.03) contained genes of ND virus. Based on homology tests and phylogenetic analysis, the virus belonged to subgenotype VIIh with an identical level of 95.34-95.86% when compared to several isolates from Indonesia. The MSL.03 ND virus has 112RRRKRF117 pattern in F0 indicatest its virulent category.