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Pemberdayaan Ibu Rumah Tangga Dalam Pemanfaatan Tanaman Obat Keluarga (TOGA) Leswara, Dianita Febrina; Mufrod; Kurniasih, Kholif Sholehah Indra
The Journal of Innovation in Community Empowerment Vol 6 No 1 (2024): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACT. Indonesian have used plants as traditional medicine to treat health problems. In general, the use of traditional medicine is considered safer than the use of modern medicine. This is because traditional medicine has relatively fewer side effects than modern medicine. However, it is still necessary to use traditional medicines accurately to minimize side effects. Empowering housewives in using Family Medicinal Plants can be used to support improving family welfare and health. For this reason, this service activity aims to: (1) increase knowledge about the scientific properties of Family Medicinal Plants among housewives, (2) increase knowledge about the procedures for planting Family Medicinal Plants among housewives, and (3) improve skills for processing Family Medicinal Plants among housewives. stairs so that it can be beneficial for health. Service activities are carried out using the lecture method to explain the scientific benefits of Family Medicinal Plants, planting Family Medicinal Plants, and processing Family Medicinal Plants. Overall service activities can be said to be good and successful, seen from the success of the target number of training participants, achievement of training objectives, achievement of planned material targets, and participant's ability to master the material to meet predetermined targets. The Empowerment of Housewives in the Use of Family Medicinal Plants community service program in Pangkah Hamlet, Bantul, Yogyakarta was able to increase participants' interest and knowledge regarding the use of family medicinal plants. KEYWORDS: Herbal; Housewife; family medicinal plants

Effect of Particle Size and Extraction Time on Total Flavonoid Level of Artemisia vulgaris Ethanol Extract Kurniasih, Kholif Sholehah Indra; Arifah, Mitsalina Fildzah; Setiyawati, Eka Ayu; Rahmah, Syafira Atika; Widianingrum, Lucia; Kholifah, Eva
Journal of Biotechnology and Natural Science Vol. 4 No. 2 (2024): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v4i2.12045

Background: Artemisia vulgaris, also referred to as Daun Baru Cina, is a herbaceous species within the Asteraceae family. Artemisia vulgaris is a traditional remedy for numerous ailments, exhibiting anti-inflammatory and antioxidant properties due to its secondary metabolites, specifically flavonoids. The extraction method is essential for extracting the desired chemical from natural sources intended for therapeutic use. The extraction process is affected by several elements, including the particle size of simplicia powder and the duration of extraction. Objective: This study aims to test the effect of differences in particle size of powdered simplicia and extraction time of Artemisia vulgaris leaves on total flavonoid levels, using 70% ethanol solvent. Researchers used a quantitative approach using a descriptive research design. In this study, a qualitative test was carried out in the form of phytochemical screening followed by a quantitative test to determine the total flavonoid levels of Artemisia vulgaris leaves. Various particle sizes (40 mesh and 80 mesh) and extraction times (12 hours and 36 hours) were used in this study. Using ultraviolet-visible spectrophotometry, the Total Flavonoid Level (TFC) of the ethanol extract of Artemisia vulgaris leaves was determined. Results: The TFC value obtained for the optimal particle size was 40 mesh, with an extraction time of 12 hours, which was 72.073 ± 1.126 mgQE / g extract. While 80 mesh, with an extraction time of 36 hours, gave a TFC value of 70.169 ± 0.480 mgQE / g extract. In addition, 70% ethanol extract of Artemisia vulgaris leaves contains secondary metabolites of phenolic alkaloids, tannins, saponins, and steroids. Conclusion: The study concludes that the quantity of simplicia powder particles and the extraction duration influence the total flavonoid concentrations in Artemisia vulgaris.

Pengaruh Sosialisasi Pengelolaan Sampah Organik dan Anorganik di Desa Jetakan Sumberagung Bantul Nurhasanah, Devika; Kurniasih, Kholif Sholehah Indra
The Journal of Innovation in Community Empowerment Vol 5 No 2 (2023): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v5i2.964

Sampah merupakan material sisa yang dihasilkan dari kegiatan dan usaha manusia. Permasalahan sampah tidak dapat dipisahkan dari kehidupan manusia, karena setiap beraktivitas berpotensi menghasilkan sampah. Pengelolaan sampah adalah suatu kegiatan yang berkesinambung dalam pengurangan dan penanganan sampah. Pemisahan sampah organik dan anorganik merupakan salah satu cara dalam penanganan sampah, terutama sampah dosmetic. Tujuan dari pengabdian kepada masyarakat ini adalah untuk meningkatkan pengetahuan masyarakat mengenai pengelolaan sampah organik maupun anorganik. Pengabdian masyarakat dilakukan di Desa Jetakan, Kecamatan Sumberagung, Kabupaten Bantul Yogyakarta dengan sasaran kegiatan ini adalah ibu-ibu rumah tangga. Metode yang dilakukan dalam pengabdian ini adalah sosialisasi menggunakan media leaflet. Pengukuran hasil kegiatan melalui analisis kuisioner terkait pemahaman mengenai persampahan. Dari hasil analisis kegiatan sosialisasi pengelolaan sampah organik dan anorganik menunjukkan terjadi peningkatan pemahaman masyarakat terhadap persampahan.

Penerapan Response Surface Methodology dalam Optimasi Proses Ekstraksi dari Artemisia vulgaris Terhadap Nilai Rendemen Kurniasih, Kholif Sholehah Indra; Firdausia, Rizqa Salsabila; Leswara, Dianita Febrina; Nurhayati, Adela; Febriana, Siti Zahara
Majalah Farmaseutik Vol 21, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v21i2.101817

Dalam beberapa tahun terakhir, telah terjadi peningkatan terhadap penggunaan bahan alam sebagai senyawa dalam proses pembuatan obat. Penggunaan bahan alam mempunyai efek samping yang cenderung lebih kecil dibandingkan dengan obat sintetik. Artemisia vulgaris memiliki kandungan metabolit sekunder yang dapat dijadikan sebagai kandidat obat bahan alam. Dalam mengisolasi senyawa metabolit tersebut diperlukan kondisi ekstraksi yang optimal untuk memberikan efek yang maksimal. Tujuan dari penelitian ini adalah melakukan optimasi kondisi proses ekstraksi pada Artemisia vulgaris sehingga diperoleh ukuran partikel, waktu maserasi serta konsentrasi pelarut yang optimal dalam mengisolasi metabolit sekunder berdasarkan atas nilai rendemen. Program Minitab 17 dengan Response Surface Methodology Box-Behnken Design digunakan dalam penelitian ini untuk memilih kondisi proses ekstraksi berdasarkan faktor-faktor yang dapat memberikan respon maksimal. Penelitian ini dilakukan dengan menggunakan variasi ukuran partikel 40, 60, dan 80 mesh, waktu maserasi 12, 24, dan 36 jam, serta variasi konsentrasi pelarut etanol 50%, 70%, dan 90%. Hasil analisis menunjukkan bahwa perbedaan nomer ayakan, waktu maserasi, dan konsentrasi pelarut dapat mempengaruhi %rendemen yang diperoleh. Diketahui nilai rendemen menggunakan nomer ayakan 40, waktu maserasi 24 jam serta konsentrasi etanol 90% memiliki %rendemen yang rendah, yaitu 0,715% jika dibandingkan dengan nomer ayakan 80, waktu maserasi 24 jam, dan konsentrasi pelarut 50% yaitu 3,42%.

The Influence of Dry Rendering and Ultrasonic?Assisted Extraction on DPPH Radical Scavenging Activity of Red Fruit (Pandanus conoideus) Arifah, Mitsalina Fildzah; Kurniasih, Kholif Sholehah Indra; Kurniawati, Endah; Erlinaningrum, Mustika; Melanie, Zaskia Nora
MEDIA ILMU KESEHATAN Vol 14 No 2 (2025): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v14i2.1471

Background: Red fruit (Pandanus conoideus) is a source of antioxidant compound components. Extraction methods can affect the antioxidant activity of the red fruit oil (RFO). Dry rendering is often used in conventional oil extraction, but it can potentially degrade antioxidant components. Currently, ultrasonic-assisted extraction (UAE) is a modern, yet underexplored method for oil extraction.Objective: This study aims to compare the extraction method on the antioxidant activity of RFO. Methods: The extraction methods included dry rendering and ultrasound-assisted extraction (UAE) using water and n-hexane as solvents. The flavonoid and phenolic contents, percentage yield, and DPPH free radical scavenging antioxidant activity were evaluated in RFO.Results: The results showed that the % yield with dry rendering was higher than in the UAE. The three extracts showed the presence of flavonoids and phenolics based on FeCl3 and AlCl3 tests. The highest antioxidant activity was in the dry rendering method (0,13% w/v) compared to UAE-water (0,98% w/v) and UAE-n hexane (1,47% w/v). Dry rendering could separate bioactive compounds with the highest % yield and inhibit DPPH free radicals with high temperatures, and without solvents. Conclusion: The dry rendering was selected as the extraction method, resulting in higher yields and antioxidant compounds in EBM compared to UAE.

Pemberdayaan Ibu Rumah Tangga Dalam Pemanfaatan Tanaman Obat Keluarga (TOGA) Leswara, Dianita Febrina; Mufrod; Kurniasih, Kholif Sholehah Indra
The Journal of Innovation in Community Empowerment Vol 6 No 1 (2024): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACT. Indonesian have used plants as traditional medicine to treat health problems. In general, the use of traditional medicine is considered safer than the use of modern medicine. This is because traditional medicine has relatively fewer side effects than modern medicine. However, it is still necessary to use traditional medicines accurately to minimize side effects. Empowering housewives in using Family Medicinal Plants can be used to support improving family welfare and health. For this reason, this service activity aims to: (1) increase knowledge about the scientific properties of Family Medicinal Plants among housewives, (2) increase knowledge about the procedures for planting Family Medicinal Plants among housewives, and (3) improve skills for processing Family Medicinal Plants among housewives. stairs so that it can be beneficial for health. Service activities are carried out using the lecture method to explain the scientific benefits of Family Medicinal Plants, planting Family Medicinal Plants, and processing Family Medicinal Plants. Overall service activities can be said to be good and successful, seen from the success of the target number of training participants, achievement of training objectives, achievement of planned material targets, and participant's ability to master the material to meet predetermined targets. The Empowerment of Housewives in the Use of Family Medicinal Plants community service program in Pangkah Hamlet, Bantul, Yogyakarta was able to increase participants' interest and knowledge regarding the use of family medicinal plants. KEYWORDS: Herbal; Housewife; family medicinal plants

Combination of PCR analysis and sequencing on cytochrome-b gene Canis lupus familiaris for halal authentication Kurniasih, Kholif Sholehah Indra; Erwanto, Yuny
Journal of Halal Science and Research Vol. 4 No. 2 (2023): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v4i2.8754

The adulteration of beef with lower-priced meat, such as dog meat, is common to get economic profit. Dog meat is one type of meat that is not halal for consumption. There are several ways in which beef can be adulterated, including the use of dog meat. Specific primers for cytochrome-b (CYTBCA3-kh) can be used to identify the presence of dog meat contamination. This study aimed to identify dog meat using these primers. After conducting conventional PCR and agarose electrophoresis tests, the specificity of the primers was confirmed. Following this, the DNA base sequence was analyzed using a sequencing method to ensure accurate identification of dog meat contamination in beef. Specific dog primers tested on cattle, pigs, wild boars, goats, chickens, rabbits, and rats were confirmed using conventional PCR and agarose gel electrophoresis. Amplicon length verification was analyzed in a silico sequencing method using MUSCLE and BLAST NCBI software. The results showed that the primer CYTBCA3-kh amplified the canine Cyt-b mt-DNA gene specifically. The amplicon length obtained was 111 base pairs (bp), with a similarity value of 99.12% with Canis lupus familiaris mitochondrion, complete genome. The specific primer CYTBCA3-kh can be used to identify dog meat contamination in meatball products for halal authentication. Keywords: CYTB gene, Halal Authentication, PCR, Sequencing, Canis lupus familiaris

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