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All Journal Pharmascience Acta Pharmaciae Indonesia : Acta Pharm Indo Jurnal Biologi Tropis Journal of Fundamental and Applied Pharmaceutical Science Jurnal IPTEK Bagi Masyarakat Jurnal Ilmiah Bakti Farmasi Journal of Biotechnology and Natural Science Jurnal Ilmiah Kesehatan Delima Parapemikir Jurnal Ilmiah Farmasi Jurnal Medika: Medika Jurnal Farmasi Galenika
Kholifah, Eva
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Formulation and Antioxidant Activity Analysis of Jotang Herb (Acmella paniculata) Extract Mask Cream Kholifah, Eva; Fitriani, Via; Shobah, Afifah Nur
Journal of Fundamental and Applied Pharmaceutical Science Vol 4, No 2 (2024): February
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jfaps.v4i2.19341

Abstract

Pollution in the environment can increase the production of reactive oxygen species (ROS), which can reduce the amount of antioxidants in the skin. Antioxidants are compounds that can inhibit oxidation reactions by binding to free radicals and reactive molecules. Herbaceous plant jotang (Acmella paniculata) has secondary metabolites such as flavonoids, alkaloids, tannins, and saponins, which can protect the internal and external organs of the body's cells from damage caused by free radicals on the skin. One of the treatment preparations used for the skin is a cream mask, as the use or application at the final level of skin care can improve cleanliness and health and stimulate and repair skin cells. This study aims to determine a formula that has a good antioxidant value using the DPPH method (2,2-diphenyl-1-pikrilhidrazyl). In this study, preparations were made with 3 formulas, namely Formula I (1% w/w), Formula II (10%w/w), and Formula 0 (Control). Evaluation of physical properties included organoleptic tests, homogeneity tests, spreadability tests, pH tests, viscosity tests, cream-type tests, and antioxidant tests. The result showed that the organoleptic test had a cream mask dosage in the form of semi-solid, distinctive jotang odor, light and dark green, had a homogeneous dosage form, spreadability around 5.1-5.7 with an average pH value of 6, had a good viscosity value and had a type of cream M/A and a high antioxidant value in formula II (10% w/w ) with an IC50 value of 21.959 μg/ml. Based on statistical tests, significant results were obtained of 0.000p (0.05) so that it can be interpreted that there was a significant difference in each concentration of jotang herb extract to the resulting IC50 value.
Literature Review: Mekanisme Kerja Obat Antidislipidemia Udin, Baha; Kholifah, Eva
Jurnal Ilmiah Bakti Farmasi Vol 6 No 1 (2021): Jurnal Ilmiah Bakti Farmasi
Publisher : Lembaga Penelitian & Pengabdian Kepada Masyarakat (LPPM) STIFI Bhakti Pertiwi Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61685/jibf.v6i1.75

Abstract

Dislipidemia merupakan kondisi tubuh manusia yang memiliki kadar lipid yang abnormal. Penggunaan obat merupakan salah satu metode yang ideal untuk menangani pasien dislipidemia. Saat ini, terdapat cukup bervariasi golongan obat yang digunakan untuk mengatasi dislipidemia dengan memiliki mekanisme kerja nya masing-masing. Berdasarkan mekanisme kerja obat antidislipidemia saat ini terdapat tujuh golongan, seperti golongan statin, fibrat dan derivatnya, bile acid sequestrants (bass), ezetimibe, asam nikotinik dan derivatnya, PCSK9 inhibitor, asam lemak omega-3. Terdapat perbedaan target dan mekanisme kerja suatu golongan obat.
Eksplorasi Toksisitas Ekstrak N-Hexan dan Aseton Telur Keong Mas (Pomacea canaliculata) Secara In Vitro dan In Silico Kholifah, Eva; Alifia A.T, Mohammad; Nur S, Afifah
Jurnal Ilmiah Bakti Farmasi Vol 7 No 1 (2022): Jurnal Ilmiah Bakti Farmasi
Publisher : Lembaga Penelitian & Pengabdian Kepada Masyarakat (LPPM) STIFI Bhakti Pertiwi Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61685/jibf.v7i1.86

Abstract

Telur keong mas merupakan salah satu hama pada tanaman padi. Namun, saat ini penanggulangan hama padi tersebut diarahkan pada pengembangan obat. Tujuan dari penelitian ini adalah untuk menganalisis potensi toksik yang ditimbulkan oleh ekstrak telur keong mas secara in silico. Kemudian dilakukan analisis secara in vitro untuk melihat potensi toksik pada larva Artemia salina denganmetode Brine Shrimp Lethality Test (BSLT). Selain itu, dilakukan skrining fitokimia dan untuk mengetahui metabolit sekunder yang terkandung dalam ekstrak telur keong mas. Hasil penelitian telur keong mas mengandung senyawa metabolit sekunder yakni alkaloid, flavonoid, steroid, saponin. Pengujian secara komputasi menunjukan senyawa yang terkandung dalam telur keong mas berpotensi menyebabkan toksik pada alga, daphnia. Namun senyawa tersebut tidak menyebabkan mutasi atau non mutagen. Namun hal ini berbanding terbalik dengan pengujian secara in vitro menggunakan artemia salina. Pengujian BSLT menggunakan pelarut aseton memiliki nilai LC50 1161,408 μg/ml dan n-heksana 1303,497 μg/ml. Berdaarkan nilai tersebut ekstrak telur keong mas (Pomacea canaliculata) dinyatakan bersifat tidak toksik terhadap Artemia salina Leach karna nilai LC50 >1000 μg/ml.
Penetapan Kadar Total Flavonoid Dan Alkaloid Ekstrak Etanol Herba Jotang (Acmella uliginosa (Sw.) Cass) Noviyanto, Fajrin; Alfiyah, Siti; Kholifah, Eva
Jurnal Pharmascience Vol 11, No 1 (2024): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i1.16840

Abstract

Jotang (Acmella uliginosa (Sw.) Cass) merupakan salah satu tanaman yang secara tradisional digunakan untuk pengobatan sakit gigi, sariawan, dan sakit tenggorokan. Pada tumbuhan jotang terdapat metabolit sekunder yaitu flavonoid yang dapat digunakan sebagai pengobatan antiinflamasi, antioksidan, antidiabetes, antibakteri, dan alkaloid dapat digunakan untuk pengobatan antibakteri, antidiare dan antiinflamasi. Tujuan penelitian ini adalah untuk mengetahui senyawa kimia ekstrak jotang, kadar total flavonoid dan kadar alkaloid yang ada dalam ekstrak jotang. Tahapan yang dilakukan pada penelitian ini yaitu pengambilan jotang, determinasi jotang, preparasi jotang, ekstraksi dengan metode maserasi, remaserasi, skrining fitokimia, analisis kadar flavonoid dan analisis kadar alkaloid total. Hasil penelitian menunjukan bahwa ekstrak etanol herba jotang  mengandung senyawa tanin, alkaloid, flavonoid dan saponin. Hasil kadar flavonoid ekstrak etanol herba jotang didapat sebesar 0,269% dan hasil kadar alkaloid total didapat sebesar 15,34%. Dapat disimpulkan bahwa pada penelitian ini mengandung senyawa flavonoid, alkaloid, saponin dan tanin. Kadar flavonoid total yang dimiliki sebesar 0,269% dan kadar alkaloid total sebesar 15,34%. Kata Kunci: Metabolit Sekunder, Maserasi, Kadar, Spektrofotometer UV-Vis, Tanin Jotang (Acmella uliginosa (Sw.) Cass) is a plant that is traditionally used to treat toothache, canker sores and sore throats. In the jotang plant there are secondary metabolites, namely flavonoids which can be used as antiinflammatory, antioxidant, antidiabetic, antibacterial, and alkaloids can be used for antibacterial, antidiarrheal and anti-inflammatory treatment. Rhe purpose of this study was to determine the chemical compounds of the jotang extract, the total levels of flavonoid, and the levels of alkaloids present in the jotang extract. The stages carried out in this study were jotang taking, jotang determination, sample preparation, extraction by maceration method, remaceration, phytochemical screening, analysis of flavonoid content, and analysis of total alkaloid content. The research results show that tannins, alkaloids, flavonoids and saponins are compound contained in the ethanol extract of jotang herbs. The flavonoid content result were 0.269% and the total alkaloid content result were 15.34%. it can be concluded that the ethanol extract of jotang herb contains flavonoids, alkaloid, saponins, and tannins. Jotang herb ethanol extract has a total flavonoid content of 0,269% and a total alkaloid content of 15.34%.
Analysis of sildenafil citrate in male stamina herbal medicines from the Magelang region, Central Java using thin-layer chromatography and UV spectrophotometry Saputra, Ilham Fahmi; Haresmita, Perdana Priya; Dianita, Puspita Septie; Kholifah, Eva
Acta Pharmaciae Indonesia Vol 12 No 1 (2024): Acta Pharmaciae Indonesia: Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2024.12.1.11964

Abstract

Background: Male stamina herbal medicines are widely consumed in Indonesia, often believed to enhance sexual performance. However, the illegal addition of medicinal chemicals (BKOs) such as sildenafil citrate in traditional herbal products poses health risks. Objective: To identify and quantify sildenafil citrate in selected herbal medicine samples using thin-layer chromatography (TLC) and UV spectrophotometry. Method: Three samples of male stamina herbal medicine were purchased and subjected to organoleptic tests. Ethanol (96%) was used for extraction, followed by TLC analysis using two mobile phases (methanol: chloroform at 4:1 and 1:1 ratios). UV spectrophotometry was employed to determine the maximum absorbance wavelength and quantify sildenafil citrate. Results: TLC analysis revealed that two samples (K and L) contained sildenafil citrate, with Rf values consistent with the standard. UV spectrophotometry confirmed the presence of sildenafil citrate at a maximum wavelength of 292.5 nm. Sample K contained 0.96%, while sample L had 13.766% sildenafil citrate. Conclusion: The presence of sildenafil citrate in two samples violates Indonesian regulations prohibiting medicinal chemicals in herbal products, raising significant safety concerns.
Effect of Particle Size and Extraction Time on Total Flavonoid Level of Artemisia vulgaris Ethanol Extract Kurniasih, Kholif Sholehah Indra; Arifah, Mitsalina Fildzah; Setiyawati, Eka Ayu; Rahmah, Syafira Atika; Widianingrum, Lucia; Kholifah, Eva
Journal of Biotechnology and Natural Science Vol. 4 No. 2 (2024): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v4i2.12045

Abstract

Background: Artemisia vulgaris, also referred to as Daun Baru Cina, is a herbaceous species within the Asteraceae family. Artemisia vulgaris is a traditional remedy for numerous ailments, exhibiting anti-inflammatory and antioxidant properties due to its secondary metabolites, specifically flavonoids. The extraction method is essential for extracting the desired chemical from natural sources intended for therapeutic use. The extraction process is affected by several elements, including the particle size of simplicia powder and the duration of extraction. Objective: This study aims to test the effect of differences in particle size of powdered simplicia and extraction time of Artemisia vulgaris leaves on total flavonoid levels, using 70% ethanol solvent. Researchers used a quantitative approach using a descriptive research design. In this study, a qualitative test was carried out in the form of phytochemical screening followed by a quantitative test to determine the total flavonoid levels of Artemisia vulgaris leaves. Various particle sizes (40 mesh and 80 mesh) and extraction times (12 hours and 36 hours) were used in this study. Using ultraviolet-visible spectrophotometry, the Total Flavonoid Level (TFC) of the ethanol extract of Artemisia vulgaris leaves was determined. Results: The TFC value obtained for the optimal particle size was 40 mesh, with an extraction time of 12 hours, which was 72.073 ± 1.126 mgQE / g extract. While 80 mesh, with an extraction time of 36 hours, gave a TFC value of 70.169 ± 0.480 mgQE / g extract. In addition, 70% ethanol extract of Artemisia vulgaris leaves contains secondary metabolites of phenolic alkaloids, tannins, saponins, and steroids. Conclusion: The study concludes that the quantity of simplicia powder particles and the extraction duration influence the total flavonoid concentrations in Artemisia vulgaris.
Acmella Uliginosa: Farmakologi, Fitokimia, Toksikologi dan Perkembangan Analog: Review Artikel Kholifah, Eva; Kurnia, Nia Marlina; Insani, Nurul
Jurnal Ilmiah Kesehatan Delima Vol. 4 No. 2 (2022): Jurnal Ilmiah Kesehatan Delima
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Sekolah Tinggi Ilmu Kesehatan Salsabila Serang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60010/jikd.v4i2.82

Abstract

Acmella uliginosa is an important medicinal plant, it is found in tropical and subtropical countries especially Indonesia, India and South America. This plant known as a toothache plant which is usually used to reduce pain associated with toothache. Various extracts and active metabolites from various parts of this plant have beneficial pharmacological activities. Several studies show Acmella Uliginosa has pharmacological activities such as antimicrobial, antipyretic, antioxidant, and anti-inflammatory. This review in detail describes the traditional uses, phytochemicals, pharmacology, toxicology and development of analogues of the active constituents of this plant. This review will help researchers to find scientific information in the future. Acmella Uliginosa adalah tanaman obat penting, ditemukan di negara-negara tropis dan subtropis terutama Indonesia, India dan Amerika Selatan. Tanaman ini dikenal sebagai tanaman sakit gigi yang biasanya digunkan untuk mengurangi rasa sakit yang berhubungan dengan sakit gigi. Bermacam-macam ekstrak dan metabolit aktif dari berbagai bagian tanaman ini memiliki aktivitas farmakologis yang bermanfaat. Beberapa penelitian menyebutkan Acmella Uliginosa memiliki aktivitas farmakologi seperti antimikroba, antipiretik, antioksidan, dan antiinflamasi. Ulasan ini secara rinci menggambarkan penggunaan tradisional, fitokimia, farmakologi, toksikologi dan perkembangan analog dari konstituen aktiv tanaman ini. Review ini akan membantu peneliti untuk mencari informasi ilmiah di masa depan.
Optimasi Formula Tablet Black Garlic Menggunakan Kombinasi Laktosa dan Avicel Ph 102 dengan Metode Simplex Lattice Design Zahroh, Siti Lailatu; Nurmay Stiani, Sofi; Kholifah, Eva
Jurnal Ilmiah Kesehatan Delima Vol. 5 No. 2 (2023): Jurnal Ilmiah Kesehatan Delima
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Sekolah Tinggi Ilmu Kesehatan Salsabila Serang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60010/jikd.v5i2.95

Abstract

The study aims to find out whether the combination of lactose and avicel pH 102 can be formulated into black garlic extract tablet in accordance with the Indonesian Pharmacopoeia, the influence of combination, and determine the optimum formulation of the two ingredients using the SLD method. Methods used in this research Black Garlic extract is made by maceration method with 96% ethanol solvent with an extract weight of each tablet of 400 mg. Black garlic extract tablets are made in three formulations with a ratio of lactose-avicel PH-102, namely FI (100%:0%), FII (50%:50%), and FIII (0%:100%). Result shows that the optimum formula obtained was 80% lactose and 20% avicel PH 102 with a total predicted value of 0.645. The greater the concentration of avicel PH 102 as a crushing material, the faster the time rate of destruction, the hardness decreases, and the fragility value rises. The higher the concentration of the filler, the longer the crushing time it will take. The results of the statistical analysis of the three formulas tested showed the absence of significant differences between flow time, hardness, and brittleness, while crushed time tests showed the presence of significant differences (p?0.05). Tujuan penelitian ini adalah kombinasi antara laktosa dan avicel pH 102 dapat diformulasikan menjadi sediaan tablet sesuai dengan Farmakope Indonesia, pengaruh tehadap kombinasi dan penentuan formulasi yang optimum dari kedua bahan tersebut dapat dilakukan dengan metode SLD. Metode dalam penelitian ini adalah Ekstrak Black Garlic dibuat dengan metode maserasi dengan pelarut etanol 96% dengan bobot ekstrak setiap tablet 400 mg. Tablet ekstrak black garlic dibuat tiga formulasi dengan perbandingan laktosa-avicel PH-102 yaitu FI (100%:0%), FII (50%:50%) dan FIII (0%:100%). Hasil penelitian menunjukkan bahwa formula optimum yang diperoleh adalah 80% laktosa dan 20% avicel PH 102 dengan total nilai prediksi 0.645. Semakin besar konsentrasi avicel PH 102 sebagai bahan penghancur, maka tingkat waktu hancur semakin cepat, kekerasan semakin turun, nilai kerapuhan semakin naik. Semakin tinggi konsentrasi pengisi, maka semakin lama waktu hancur yang dibutuhkan. Hasil analisis statistik dari ketiga formula yang diuji menunjukkan tidak adanya perbedaan yang signifikan antara waktu alir, kekerasan dan kerapuhan, sedangkan untuk uji waktu hancur menunjukkan adanya perbedaan yang signifikan (p?0.05).
Identifikasi Flavonoid total dan Vitamin C pada Ekstrak buah Alkesa (Pouteria campechiana (Kunth) Baehni) menggunakan Spektrofotometri UV-VIS Kholifah, Eva; Agustian, Lolo; Noviyanto, Fajrin
Jurnal Ilmiah Kesehatan Delima Vol. 6 No. 2 (2024): Jurnal Ilmiah Kesehatan Delima
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Sekolah Tinggi Ilmu Kesehatan Salsabila Serang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60010/jikd.v6i2.120

Abstract

Buah Alkesa (Pouteria campechiana (Kunth) Baehni) adalah buah yang mengandung antioksidan yang sangat tinggi buah alkesa ini menjadi sumber karbohidrat, vitamin, mineral, serat dan mengandung senyawa flavonoid, saponin, dan vitamin C. Tujuan penelitian ini adalah untuk mengetahui kadar vitamin C dan flavonoid total pada ekstrak buah alkesa dengan menggunakan metode spektrofotometer UV-Vis. Hasil penelitian ini menunjukan kandungan metabolit sekunder buah alkesa adalah flavonoid dan saponin, kadar vitamin C yang diperoleh pada ekstrak buah alkesa sebesar 1,2816% sedangkan kadar flavonoid total sebesar 0,2533%. Pada penelitian ini menunjukan buah alkesah mengandung flavonoid dan vitamin c yang bisa dikembangkan dalam produk farmasi.
UJI TOKSISITAS dan ANALISIS PROFIL FARMAKOKINETIK EKSTRAK ETANOL BLACK GARLIC dan TABLET BLACK GARLIC SECARA IN VITRO dan IN SILICO Kholifah, Eva
Parapemikir : Jurnal Ilmiah Farmasi Vol 13, No 2 (2024): Parapemikir : Jurnal Ilmiah Farmasi
Publisher : Pusat Penelitian dan Pengabdian Masyarakat Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/pjif.v13i2.6851

Abstract

Toksisitas merupakan masalah utama dalam pengembangan obat baru. Pengujian toksisitas bertujuan untuk melihat efek toksik terhadap organ yang ditimbulkan oleh senyawa obat. Penelitian ini bertujuan untuk mengetahui toksisitas dari Ekstrak etanol Black garlic dan tablet black garlic terhadap Artemia salina leach menggunakan metode Brine Shimp Lethal Test dan analisis profil farmakokinetik secara in silico. Hasil penelitian menunjukkan bahwa Tablet black garlic memiliki metabolit sekunder flavonoid dan fenolik dan nilai toksisitas senyawa black garlic, tablet formula 1 dan 2 berturut-turut sebesar 18866,4615 mg/L, 44666,6363 mg/L, 23704 6190 mg/L. Analisis farmakokinetik menunjukan senyawa yang terkandung dalam ekstrak black garlic menunjukan diabsorpsi tinggi di dalam saluran GI dan potensi terjadinya interaksi obat – obat pada  fase farmakokinetik sangat kecil, sehingga dalam hal ini formulasi ekstrak black garlic menjadi sediaan tablet dapat terus dikembangkan.
Co-Authors Agustian, Lolo Agustin, Siti Ahmaddhani, Surya Alfarizqi, Asep Salman Alfiyah, Siti Alifia A.T, Mohammad Anton Anton Arifah, Mitsalina Fildzah Asep Aziya, Hasna Baha Udin Cahya Skania, Pratiwi endah, endah Fajrin Noviyanto, Fajrin Fitriani, Via Ismiyati, Renditya Kurnia, Nia Marlina Kurniasih, Kholif Sholehah Indra Lukmanul Hakim, Imam Maulana, Adrian Maulana, Balqis Nabilah, Syala Nabillah, Deya Adiby Nasiah Nasiah, Nasiah Nur Insani Nur S, Afifah Perdana Priya Haresmita, Perdana Priya Puspita Septie Dianita, Puspita Septie R, Asti Yunia Rahmah, Syafira Atika Saputra, Ilham Fahmi Setiyawati, Eka Ayu Shobah, Afifah Nur Stiani, Sofi Nurmay Sudarto, Teguh Suhaemi, Muhamad Widianingrum, Lucia Yusransyah, Yusransyah Zahroh, Siti Lailatu