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All Journal Jurnal Kartika Kimia Helium: Journal of Science and Applied Chemistry al Kimiya : Jurnal Ilmu Kimia dan Terapan
Widyatama, Awang
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Materials Science & Nanotechnology

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Air Quality Analysis of Nitrogen Dioxide and Ozone (NO2 and O3) In The Chemistry Laboratory of Universitas Pakuan Bogor Pekerti, Unggul Tri; Widyatama, Awang; Imawati, Naufa Dea; Syahputri, Yulian
Helium: Journal of Science and Applied Chemistry Vol 3, No 2 (2023): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v3i2.9470

Abstract

Universitas Pakuan is one of the universities in Bogor which has a chemistry education laboratory for practical purposes. In this study, the room tested is the instrument room and preparation room. Air testing of this work environment refers to the Minister of Manpower Regulation No. 05 of  2018. The test results for the instrument room and preparation room found that the levels of nitrogen dioxide (NO2) and ozone (O3) in the two rooms do not show any indication of  NO2 and O3 pollution with result values below the detection limit testing (0.0066 ppm and 0.0491 ppm).
The Utilization of Two Commercial Indonesian Fertilizers as Microalgal Growth Medium for Chlorella pyrenoidosa and Botryococcus braunii Cultivation Widyatama, Awang; Al Fajry, Zaidan; Widyatama, Satria Aqilla; Mulyati, Ade Heri; Stiawan, Elva
Jurnal Kartika Kimia Vol 7 No 1 (2024): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v7i1.252

Abstract

In the current trend of the upstream stage of microalgal bioprocessing studies, many works mainly focused on optimizing microalgal cultivation parameters for obtaining a high yield of either biomass or a particular metabolite. Meanwhile, efforts to alternatively provide affordable growth medium are considered still limited to be performed. This study aims to provide an alternative microalgal growth medium from commercial fertilizers and supplements provided by a particular Indonesian company. Our proposed microalgal growth medium was developed by considering the chemical contents of the products with the essential components of an established Guillard f/2 microalgal growth medium. By employing the developed growth medium in Chlorella pyrenoidosa and Botryococcus braunii cultivation, similar growth profiles were shown in both green microalgae with optimum time for biomass harvesting encouraged within two weeks of cultivation. Regarding biomass productivity, 0.19 g and 0.20 of dried biomass were averagely obtained from 1 L cultures of Chlorella pyrenoidosa and Botryococcus braunii, respectively. This finding implies that two Indonesian fertilizer products could be utilized as the main ingredient of moderate performance of green microalgal growth medium.
Biomass Contents Of Chlorella Pyrenoidosa Cultivated In Cobalt-Less Fertilizer-Enriched Saline Water Widyatama, Awang; Widyatama, Satria Aqilla; Fajry, Zaidan Al; Atmadi, Atmadi; Mulyawati, Ade Heri; Stiawan, Elva
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol. 12 No. 1 (2025): June 2025
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v12i1.39468

Abstract

This research aims to provide some information regarding the metabolite contents of the green microalgae Chlorella pyrenoidosa cultivated in designed saline water enriched with affordable commercial fertilizer products in Indonesia. Chlorella pyrenoidosa growth conditions and bioactive compounds were obtained through cultivation and extraction processes. The microalga growth in two different conditions   was analyzed based on growth curves between microalgae cultivated in a Guillard f/2 medium   and the Cobalt-less fertilizer-enriched saline water.   The biomass was profiled using UPLC-QToF-MS and DPPH assay. As a result, Chlorella pyrenoidosa cultures were normally grown on fertilizer-enriched saline water, but with a lower yield performance than those cultivated in the Guillard f/2 medium. The UPLC-QToF-MS result implies that several peptide and lipid derivatives were detected in the harvested microalgal biomass, with the peptide derivatives as the dominant compounds. The limited antioxidant performance was displayed by the untreated biomass extract. This finding implies that several optimizations are still required in the upstream and downstream stages in culturing Chlorella pyrenoidosa by using an uncommon growth medium.
Co-Authors Ade Heri Mulyati, Ade Heri Al Fajry, Zaidan Atmadi, Atmadi Elva Stiawan Fajry, Zaidan Al Imawati, Naufa Dea Mulyawati, Ade Heri Pekerti, Unggul Tri Widyatama, Satria Aqilla Yulian Syahputri