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Identification of Tropical Marine Diatom Chaetoceros dayaensis CBO from Bokor Island through Morphology and Genetic Marker Analysis Stiawan, Elva; Sutomo, Sutomo; Sapulete, Sherly; Nurachman, Zeily; Rachmayanti, Yanti
Indonesian Journal of Chemical Studies Vol. 1 No. 1 (2022): Indones. J. Chem. Stud., June 2022
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.509 KB) | DOI: 10.55749/ijcs.v1i1.2

Abstract

Microalgae are well-recognized by many researchers working on bioenergy resources due to its capability to produce high amounts of lipids as biodiesel precursors. Diatoms as a group of microalgae that specifically categorized by their silica valve could be more attractive to the researchers because in addition to high-lipid content, their silica features can be also utilized for wider applications, or used for diatoms bioproduct purification. Identification of some diatom species, such as the diatom genus Chaetoceros, as an initial step in diatom studies were difficult to accurately perform because of the unique morphology of silica frustules. Meanwhile, some candidate universal genetic markers of microalgae could also be used for the identification of diatoms with brittle frustules. In this research, we aimed to identify isolate CBO of tropical marine Chaetoceros CBO strain using morphological and genetic approaches. The isolate CBO of Chaetoceros sp. CBO was successfully obtained from sea region of Bokor Island, Kepulauan Seribu, Jakarta, and cultivated in our laboratory as culture stock for further use. Better resolution of the Chaetoceros CBO identification until species level was shown by genetic analysis toward two proposed gene markers for microalgae (rbcL-3P and V4 region of 18S rDNA) rather than by morphological. Specific DNA region for this specimen was found in V4 region of 18S rDNA genetic marker that could be recognized by MspA1I restriction enzyme. Hence, the RFLP (restriction fragment length polymorphism) method could be also used as an initial diagnostic tool for identification of this tropical marine Chaetoceros dayaensis CBO and for stock culture labelling purposes.
Evaluation of The Biochemical Contents in Guillard f/2 and Walne Growth Medium to Fulfill the Animal-Free Aspects of Microalgal Bioprocessing Stiawan, Elva
Indonesian Journal of Chemical Studies Vol. 1 No. 2 (2022): Indones. J. Chem. Stud., December 2022
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.646 KB) | DOI: 10.55749/ijcs.v1i2.16

Abstract

Marine microalgae with their diverse biomolecule contents could be used as potential sources of food, cosmetics, and pharmaceutical ingredients. In accordance with regulations in some countries and to competitively engage huge numbers of consumers, microalgae-based products should be properly manufactured using non-animal-derived materials. As a cultivable microorganism using a scalable bioreactor technique, consideration of the origin of the material used in the upstream process of marine microalgae was inevitable. Currently, the material origin of chemical contents within common artificial microalgal seawater medium had not been evaluated. This article evaluated Guillard f/2 and Walne medium as common artificial microalgal nutrients used in marine microalgal bioprocess-related activities. The risk assessment results showed that the largest portion of Guillard f/2 and Walne media were inorganic salts considered as low, while the remaining biochemical contents of vitamins were categorized as high risk due to their relatively complex chemical synthesis and enzymatic stages during the manufacturing process. As a suggestion, several plant-based bioproducts were proposed as alternative sources to substitute related biochemical actions to fulfill non-animal-origin aspects in the initial stages of the bioprocessing of marine microalgal-based products.
The Utilization of Two Commercial Indonesian Fertilizers as Microalgal Growth Medium for Chlorella pyrenoidosa and Botryococcus braunii Cultivation Widyatama, Awang; Al Fajry, Zaidan; Widyatama, Satria Aqilla; Mulyati, Ade Heri; Stiawan, Elva
Jurnal Kartika Kimia Vol 7 No 1 (2024): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v7i1.252

Abstract

In the current trend of the upstream stage of microalgal bioprocessing studies, many works mainly focused on optimizing microalgal cultivation parameters for obtaining a high yield of either biomass or a particular metabolite. Meanwhile, efforts to alternatively provide affordable growth medium are considered still limited to be performed. This study aims to provide an alternative microalgal growth medium from commercial fertilizers and supplements provided by a particular Indonesian company. Our proposed microalgal growth medium was developed by considering the chemical contents of the products with the essential components of an established Guillard f/2 microalgal growth medium. By employing the developed growth medium in Chlorella pyrenoidosa and Botryococcus braunii cultivation, similar growth profiles were shown in both green microalgae with optimum time for biomass harvesting encouraged within two weeks of cultivation. Regarding biomass productivity, 0.19 g and 0.20 of dried biomass were averagely obtained from 1 L cultures of Chlorella pyrenoidosa and Botryococcus braunii, respectively. This finding implies that two Indonesian fertilizer products could be utilized as the main ingredient of moderate performance of green microalgal growth medium.
Enriching of society’s understanding of command-oriented leadership through an interactive virtual discussion Fahri, Mirad; Stiawan, Elva; Ariyanti, Dita; Basuki, Rahmat; Apriliyanto, Yusuf Bramastya; Hasibuan, Anggi Khairina Hanum; Kurniadi, Tedi; Putra, M. Sulthon Nurharmansyah; Septaningsih, Dewi; Tiarani, Sekar Ilma; Mayori, Ersha; Wiana, Regita Andriani; Yunita, Amalia; Nurrosyid, Naufan; Prasojo, Agus Eko; Putri, Riyanti
Journal of Community Service and Empowerment Vol. 5 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v5i2.27961

Abstract

In the post-pandemic era, many interactive activities have been flexibly generated through virtual conditions. Accordingly, some problems could appear in terms of leadership, such as difficulties in maintaining the audience’s attention during the event. Thus, the utilization of a command-based leadership style that could straightforwardly maintain audience focus ought to be considered to be adopted. However, both the command-based leadership style and its prospective aspects have not been widely understood by society. This community service aims to deliver a proper understanding of the command-based leadership style through interactive virtual discussion between experts in teaching activities of defense-based science and society consisting of Indonesian teachers, high school students as well as academics. Statistical results of pre-test and post-test scores showed enhancement of the participants’ understanding of command-based leadership. Hopefully, some relevant aspects of their new perspective of command-based leadership can be properly adopted and effectively implemented to answer current challenges, such as in terms of managing people in virtual conditions. 
Safety induction for agricultural workers in particular areas of Gadog Village in handling hazardous agrochemical exposures Xaviera, Allodya Nadra; Hijrianisa, Alya; Lubis, Astrid Blandina Cynthia; Stiawan, Elva; Gunaryo, G.
Journal of Community Service and Empowerment Vol. 5 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v5i2.30180

Abstract

An integrated program of community service and chemical teaching has been conducted to deliver proper information to handle chemical agents that are commonly used in agricultural activities. This initiative addresses the limitation of chemical understanding among local farmers and livestock breeders regarding the safety aspect of common agrochemicals, leading to either health risks or environmental problems. Knowledge regarding agrochemical handling and disposal was delivered through presentations, discussions, and hands-on practices. Accordingly, through this community service, we encouraged agricultural workers with a basic understanding of the chemical properties of hazardous agrochemicals and the safety induction in handling and disposing of those compounds. Based on the activities carried out, the results of the pre-test and post-test showed an increase of 62% in farmers' understanding of hazardous chemicals and proper handling of these chemicals. In addition to providing some beneficial scientific information to enhance agricultural workers’ safety in their working area, an activity related to education and training of agrochemicals could increase peoples’ awareness toward hazardous chemicals that would be still freely commercialized, for example, in terms of mitigating risks associated with chemical threats.
Socialization of CBRN-E Threat and Its Anticipation Toward Students of a Particular Senior High School in Bogor Area Stiawan, Elva; Sari, Dini Rusfita; Gita, Mutiara; Atmadi
Jurnal Kesehatan Tambusai Vol. 5 No. 3 (2024): SEPTEMBER 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v5i3.33269

Abstract

Kesadaran bela negara sudah sepatutnya disadari secara luas dan khususnya diterapkan oleh generasi muda Indonesia saat ini. Gagasan tersebut hendaknya disampaikan secara strategis kepada siswa-siswa SMA untuk mempersiapkan sumber daya manusia berkualitas yang mendukung pertahanan negara, khususnya dalam mengantisipasi ancaman Bahan Kimia, Biologi, Radiologi, Nuklir, dan Bahan Peledak (CBRN-E). Namun informasi mengenai pemahaman CBRN-E pada tingkat siswa SMA di Indonesia masih terbatas. Pengabdian masyarakat ini bertujuan untuk memberikan profil khusus pemahaman siswa SMA setempat terhadap ancaman CBRN-E sebagai wawasan program selanjutnya dalam mengantisipasi ancaman CBRN-E. Selain melakukan sosialisasi kepada siswa SMA di salah satu SMA tertentu di Kabupaten Bogor, kami menganalisis secara singkat pemahaman awal mereka terhadap isu-isu terkait pertahanan dan topik terkait kimia melalui pre-test kuesioner yang dirancang. Hasil studi pendahuluan ini menunjukkan bahwa pengetahuan mereka tentang ancaman CBRN-E harus diperkuat. Menariknya, sebagian besar peserta mempunyai persepsi positif mengenai preferensi karir mereka untuk mendukung sektor pertahanan negara, misalnya minat mereka untuk berkontribusi di bidang kimia untuk memperkuat pertahanan negara. Temuan ini menyiratkan mendorong pemangku kepentingan pendidikan untuk berkolaborasi secara strategis dengan lembaga pertahanan negara untuk mengintegrasikan materi pembelajaran terkait CBRN-E ke dalam kurikulum yang ada.
Exploring the Potential of Carbon-based Radar Absorbing Material Innovations Fahri, Mirad; Bolilanga, Patricya Inggrid Wilhelmina; Gunaryo, Gunaryo; Stiawan, Elva; Kurniadi, Tedi
Indonesian Journal of Chemical Studies Vol. 3 No. 2 (2024): Indones. J. Chem. Stud., December 2024
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/ijcs.v3i2.56

Abstract

This review explored the potential of carbon-based radar-absorbing materials (RAM), which had gained significant attention due to their superior properties and performance. In response to the growing demand for stealth technology in the military and civilian sectors, traditional radar-absorbing materials encountered limitations: weight, cost, and effectiveness. Carbon-based materials, such as carbon nanotubes, graphene, and various composites, offered lightweight, flexible, and tunable solutions that enhanced electromagnetic wave absorption across a wide frequency range. This paper examined the underlying mechanisms of radar wave absorption in carbon-based materials, highlighting their advantages over conventional options. In addition, recent advancements in fabrication techniques, including 3D printing and hybrid composite development, were also discussed, emphasizing their role in optimizing performance and sustainability. By synthesizing current research findings, this review aimed to provide a comprehensive understanding of the carbon-based RAM potential in advancing the future of stealth technology. Ultimately, this study presented insights that contribute to the continuing investigation in advanced materials science, suggesting a potential way to develop materials that can enhance radar absorption capabilities and extend their applications in modern technology.
Brief Virtual Observation Revealed the Possibility of Trade in Organophosphate-based Pesticides through E-commerce Stiawan, Elva; Nurhalimah, Siti; Ananda, Dea Dwi; MS, M. Adrian Rezki Anggara; Tayon, Felix
Jurnal Pengabdian Masyarakat AKA Vol 4, No 1 (2024): Juni 2024
Publisher : Politeknik AKA Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55075/jpm-aka.v4i1.225

Abstract

Reactive organophosphates (OPs) could be utilized as deadly pesticides due to their nerve agent properties for several organisms. Although the utilization of these compounds has been strictly regulated, monitoring its trading activities, particularly through e-commerce, could be quite challenging. There is a lack of information on whether the pesticide products containing OPs or slightly featured with similar phosphates groups exist or are un-existed in the online stores in Indonesia. This community service aims to briefly report the possibility of trade in phosphorous-based pesticides through online stores in Indonesia. We demonstrated virtual observation to obtain the list of phosphorous-based pesticides from several e-commerce in Indonesia. We detected 24 pesticides displayed on some popular e-commerce with particular phosphate groups in the active substance, i.e., Acephate, Chlorpyrifos, Dimethoate, Diazinon, Profenofos, and Triazofos. The finding could provide insight for those online stores to establish particular rules to monitor and control trading activities of agrochemicals that could be illegally used or further processed for any activities related to Chemical, Biological, Radiological, and Nuclear and Explosives (CBRN-E) threats.
Training of HOTS integration in elementary school level mathematics teaching as an effort to strengthen state defense awareness Stiawan, Elva; Basuki, Rahmat; Prawira, Ajeng Arimby Setyaningsih Putri Yan
Journal of Community Service and Empowerment Vol. 5 No. 3 (2024): December
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v5i3.32943

Abstract

Strengthening state defense awareness could be supported by the existence of educators from remote and border areas who have adequate performance of high-order thinking skills (HOTS). Currently, HOTS has not been consistently introduced in mathematics learning for teachers in remote areas. This study is a part of the teachers’ development program concerned with delivering effective methods in teaching mathematics at the elementary school level and gaining teachers’ State Defense Awareness to provide educators’ perspectives regarding HOTS implementation in mathematics learning in remote areas of Indonesia. Data was collected by giving questionnaires to the teachers as pre-tests and post-tests. The results showed that the teachers of some remote areas recognized the advantages as well as the difficulties of HOTS integration in learning activities. Teachers' understanding of applying HOTS in mathematics teaching could be slightly increased through comprehensive training regarding HOTS. It was also found that teachers from remote and border areas are still required to be more encouraged to implement and develop HOTS in mathematics learning through training in education research activities.
Formulation of Digested Beverage Cans and Iron Plate Wastes as a Coagulant for Adequate Hygiene of Fresh River Water Gita, Mutiara; Stiawan, Elva; Renta, Hotma; Kuntjahjono, Mayang Fauziah Putri; Lestari, Aura Puja
Indonesian Journal of Chemical Studies Vol. 4 No. 1 (2025): Indones. J. Chem. Stud., June 2025
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/ijcs.v4i1.57

Abstract

In terms of hygiene and sanitation, ensuring the availability of qualified water for those purposes remains challenging to perform under certain conditions. Accordingly, efforts to provide simple water processing technology are ongoing and innovatively developed. This study displayed an innovative approach to producing coagulants for water processing by utilizing metal salts obtained synthetically from used beverage cans and iron plates through the electrolysis principle and characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and Scanning Electron Microscopy - The Energy Dispersive X-ray (SEM-EDX), subsequently. After mixing with calcium hypochlorite and adding to water sample, subsequently, the coagulant showed the ability to reduce the turbidity level and several categories of impurities, i.e., nitrate, nitrite, dissolved Manganese, Cr6+ ion, and microbial levels. Future research and development in formulating coagulants derived from digested beverage cans and iron plate wastes hold significant potential to advance sustainable and efficient water treatment technologies, ensuring improved hygienic quality of fresh river water while contributing to waste valorization and environmental protection.