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Identification of Tropical Marine Diatom Chaetoceros dayaensis CBO from Bokor Island through Morphology and Genetic Marker Analysis Stiawan, Elva; Sutomo, Sutomo; Sapulete, Sherly; Nurachman, Zeily; Rachmayanti, Yanti
Indonesian Journal of Chemical Studies Vol. 1 No. 1 (2022): Indones. J. Chem. Stud., June 2022
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.509 KB) | DOI: 10.55749/ijcs.v1i1.2

Abstract

Microalgae are well-recognized by many researchers working on bioenergy resources due to its capability to produce high amounts of lipids as biodiesel precursors. Diatoms as a group of microalgae that specifically categorized by their silica valve could be more attractive to the researchers because in addition to high-lipid content, their silica features can be also utilized for wider applications, or used for diatoms bioproduct purification. Identification of some diatom species, such as the diatom genus Chaetoceros, as an initial step in diatom studies were difficult to accurately perform because of the unique morphology of silica frustules. Meanwhile, some candidate universal genetic markers of microalgae could also be used for the identification of diatoms with brittle frustules. In this research, we aimed to identify isolate CBO of tropical marine Chaetoceros CBO strain using morphological and genetic approaches. The isolate CBO of Chaetoceros sp. CBO was successfully obtained from sea region of Bokor Island, Kepulauan Seribu, Jakarta, and cultivated in our laboratory as culture stock for further use. Better resolution of the Chaetoceros CBO identification until species level was shown by genetic analysis toward two proposed gene markers for microalgae (rbcL-3P and V4 region of 18S rDNA) rather than by morphological. Specific DNA region for this specimen was found in V4 region of 18S rDNA genetic marker that could be recognized by MspA1I restriction enzyme. Hence, the RFLP (restriction fragment length polymorphism) method could be also used as an initial diagnostic tool for identification of this tropical marine Chaetoceros dayaensis CBO and for stock culture labelling purposes.
Evaluation of The Biochemical Contents in Guillard f/2 and Walne Growth Medium to Fulfill the Animal-Free Aspects of Microalgal Bioprocessing Stiawan, Elva
Indonesian Journal of Chemical Studies Vol. 1 No. 2 (2022): Indones. J. Chem. Stud., December 2022
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.646 KB) | DOI: 10.55749/ijcs.v1i2.16

Abstract

Marine microalgae with their diverse biomolecule contents could be used as potential sources of food, cosmetics, and pharmaceutical ingredients. In accordance with regulations in some countries and to competitively engage huge numbers of consumers, microalgae-based products should be properly manufactured using non-animal-derived materials. As a cultivable microorganism using a scalable bioreactor technique, consideration of the origin of the material used in the upstream process of marine microalgae was inevitable. Currently, the material origin of chemical contents within common artificial microalgal seawater medium had not been evaluated. This article evaluated Guillard f/2 and Walne medium as common artificial microalgal nutrients used in marine microalgal bioprocess-related activities. The risk assessment results showed that the largest portion of Guillard f/2 and Walne media were inorganic salts considered as low, while the remaining biochemical contents of vitamins were categorized as high risk due to their relatively complex chemical synthesis and enzymatic stages during the manufacturing process. As a suggestion, several plant-based bioproducts were proposed as alternative sources to substitute related biochemical actions to fulfill non-animal-origin aspects in the initial stages of the bioprocessing of marine microalgal-based products.
The Utilization of Two Commercial Indonesian Fertilizers as Microalgal Growth Medium for Chlorella pyrenoidosa and Botryococcus braunii Cultivation Widyatama, Awang; Al Fajry, Zaidan; Widyatama, Satria Aqilla; Mulyati, Ade Heri; Stiawan, Elva
Jurnal Kartika Kimia Vol 7 No 1 (2024): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v7i1.252

Abstract

In the current trend of the upstream stage of microalgal bioprocessing studies, many works mainly focused on optimizing microalgal cultivation parameters for obtaining a high yield of either biomass or a particular metabolite. Meanwhile, efforts to alternatively provide affordable growth medium are considered still limited to be performed. This study aims to provide an alternative microalgal growth medium from commercial fertilizers and supplements provided by a particular Indonesian company. Our proposed microalgal growth medium was developed by considering the chemical contents of the products with the essential components of an established Guillard f/2 microalgal growth medium. By employing the developed growth medium in Chlorella pyrenoidosa and Botryococcus braunii cultivation, similar growth profiles were shown in both green microalgae with optimum time for biomass harvesting encouraged within two weeks of cultivation. Regarding biomass productivity, 0.19 g and 0.20 of dried biomass were averagely obtained from 1 L cultures of Chlorella pyrenoidosa and Botryococcus braunii, respectively. This finding implies that two Indonesian fertilizer products could be utilized as the main ingredient of moderate performance of green microalgal growth medium.
Socialization of CBRN-E Threat and Its Anticipation Toward Students of a Particular Senior High School in Bogor Area Stiawan, Elva; Sari, Dini Rusfita; Gita, Mutiara; Atmadi
Jurnal Kesehatan Tambusai Vol. 5 No. 3 (2024): SEPTEMBER 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v5i3.33269

Abstract

Kesadaran bela negara sudah sepatutnya disadari secara luas dan khususnya diterapkan oleh generasi muda Indonesia saat ini. Gagasan tersebut hendaknya disampaikan secara strategis kepada siswa-siswa SMA untuk mempersiapkan sumber daya manusia berkualitas yang mendukung pertahanan negara, khususnya dalam mengantisipasi ancaman Bahan Kimia, Biologi, Radiologi, Nuklir, dan Bahan Peledak (CBRN-E). Namun informasi mengenai pemahaman CBRN-E pada tingkat siswa SMA di Indonesia masih terbatas. Pengabdian masyarakat ini bertujuan untuk memberikan profil khusus pemahaman siswa SMA setempat terhadap ancaman CBRN-E sebagai wawasan program selanjutnya dalam mengantisipasi ancaman CBRN-E. Selain melakukan sosialisasi kepada siswa SMA di salah satu SMA tertentu di Kabupaten Bogor, kami menganalisis secara singkat pemahaman awal mereka terhadap isu-isu terkait pertahanan dan topik terkait kimia melalui pre-test kuesioner yang dirancang. Hasil studi pendahuluan ini menunjukkan bahwa pengetahuan mereka tentang ancaman CBRN-E harus diperkuat. Menariknya, sebagian besar peserta mempunyai persepsi positif mengenai preferensi karir mereka untuk mendukung sektor pertahanan negara, misalnya minat mereka untuk berkontribusi di bidang kimia untuk memperkuat pertahanan negara. Temuan ini menyiratkan mendorong pemangku kepentingan pendidikan untuk berkolaborasi secara strategis dengan lembaga pertahanan negara untuk mengintegrasikan materi pembelajaran terkait CBRN-E ke dalam kurikulum yang ada.
Exploring the Potential of Carbon-based Radar Absorbing Material Innovations Fahri, Mirad; Bolilanga, Patricya Inggrid Wilhelmina; Gunaryo, Gunaryo; Stiawan, Elva; Kurniadi, Tedi
Indonesian Journal of Chemical Studies Vol. 3 No. 2 (2024): Indones. J. Chem. Stud., December 2024
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/ijcs.v3i2.56

Abstract

This review explored the potential of carbon-based radar-absorbing materials (RAM), which had gained significant attention due to their superior properties and performance. In response to the growing demand for stealth technology in the military and civilian sectors, traditional radar-absorbing materials encountered limitations: weight, cost, and effectiveness. Carbon-based materials, such as carbon nanotubes, graphene, and various composites, offered lightweight, flexible, and tunable solutions that enhanced electromagnetic wave absorption across a wide frequency range. This paper examined the underlying mechanisms of radar wave absorption in carbon-based materials, highlighting their advantages over conventional options. In addition, recent advancements in fabrication techniques, including 3D printing and hybrid composite development, were also discussed, emphasizing their role in optimizing performance and sustainability. By synthesizing current research findings, this review aimed to provide a comprehensive understanding of the carbon-based RAM potential in advancing the future of stealth technology. Ultimately, this study presented insights that contribute to the continuing investigation in advanced materials science, suggesting a potential way to develop materials that can enhance radar absorption capabilities and extend their applications in modern technology.
Brief Virtual Observation Revealed the Possibility of Trade in Organophosphate-based Pesticides through E-commerce Stiawan, Elva; Nurhalimah, Siti; Ananda, Dea Dwi; MS, M. Adrian Rezki Anggara; Tayon, Felix
Jurnal Pengabdian Masyarakat AKA Vol 4, No 1 (2024): Juni 2024
Publisher : Politeknik AKA Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55075/jpm-aka.v4i1.225

Abstract

Reactive organophosphates (OPs) could be utilized as deadly pesticides due to their nerve agent properties for several organisms. Although the utilization of these compounds has been strictly regulated, monitoring its trading activities, particularly through e-commerce, could be quite challenging. There is a lack of information on whether the pesticide products containing OPs or slightly featured with similar phosphates groups exist or are un-existed in the online stores in Indonesia. This community service aims to briefly report the possibility of trade in phosphorous-based pesticides through online stores in Indonesia. We demonstrated virtual observation to obtain the list of phosphorous-based pesticides from several e-commerce in Indonesia. We detected 24 pesticides displayed on some popular e-commerce with particular phosphate groups in the active substance, i.e., Acephate, Chlorpyrifos, Dimethoate, Diazinon, Profenofos, and Triazofos. The finding could provide insight for those online stores to establish particular rules to monitor and control trading activities of agrochemicals that could be illegally used or further processed for any activities related to Chemical, Biological, Radiological, and Nuclear and Explosives (CBRN-E) threats.
Formulation of Digested Beverage Cans and Iron Plate Wastes as a Coagulant for Adequate Hygiene of Fresh River Water Gita, Mutiara; Stiawan, Elva; Renta, Hotma; Kuntjahjono, Mayang Fauziah Putri; Lestari, Aura Puja
Indonesian Journal of Chemical Studies Vol. 4 No. 1 (2025): Indones. J. Chem. Stud., June 2025
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/ijcs.v4i1.57

Abstract

In terms of hygiene and sanitation, ensuring the availability of qualified water for those purposes remains challenging to perform under certain conditions. Accordingly, efforts to provide simple water processing technology are ongoing and innovatively developed. This study displayed an innovative approach to producing coagulants for water processing by utilizing metal salts obtained synthetically from used beverage cans and iron plates through the electrolysis principle and characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and Scanning Electron Microscopy - The Energy Dispersive X-ray (SEM-EDX), subsequently. After mixing with calcium hypochlorite and adding to water sample, subsequently, the coagulant showed the ability to reduce the turbidity level and several categories of impurities, i.e., nitrate, nitrite, dissolved Manganese, Cr6+ ion, and microbial levels. Future research and development in formulating coagulants derived from digested beverage cans and iron plate wastes hold significant potential to advance sustainable and efficient water treatment technologies, ensuring improved hygienic quality of fresh river water while contributing to waste valorization and environmental protection.
Sosialisasi Lingkungan Bersih serta Pengelolaan Instalasi Pengolahan Air Limbah (IPAL) di Desa Muara Angke Minandre Wiratama; Gunaryo; Mentari Zikri Anty; Mirad Fahri; Anggi Khairina H H; Elva Stiawan; Rahmat Basuki; M. Sulthon Nurharmansyah P; Tedi Kurniadi; Dewi Anggraini S; Yusuf Bramastya A; Azimatur Rahmi; Aura Puja Lestari; Moch. Rizki Fajri
Babakti: Journal of Community Engangement Vol. 2 No. 1 (2025): April
Publisher : Universitas Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35706/babakti.v2i1.155

Abstract

Kesadaran Kebersihan dan Pengelolaan Sampah yang baik merupakan salah satu kebiasaan yang dapat berdampak pada kesehatan yang lebih baik. Kepedulian yang minim dalam menjaga lingkungan yang bersih diprediksi akan menimbulkan dampak buruk bagi lingkungan dan masyarakat. Selain itu, pengelolaan air bersih pada lingkungan merupakan salah satu hal yang perlu diperhatikan. Pengetahuan tentang pengolahan air bersih melalui Instalasi Pengelolaan Air Limbah (IPAL) akan memberikan dampak positif bagi keberlansungan air bersih di lingkungan masyarakat. Pengabdian kepada masyarakat ini dilakukan dengan Sosialisasi melalui pendekatan modal sosial. Pendekatan modal sosial dilakukan dalam pelaksanaan pengabdian kepada masyarakat dimana pendekatan ini digunakan sebagai wadah sosialisasi menjaga kebersihan lingkungan dan pengetahuan dalam pengolahan air bersih bagi msayarakat di Desa Muara Angke. Pengabdian kepada Masyarakat ini bertujuan untuk mensosialisasikan cara mengelola dan mengkategorikan macam-macam sampah, pengelolaan air bersih, dan desalinasi air yang dilakukan di daerah Muara Angke, Kecamatan Penjaringan, Jakarta Utara. Data dikumpulkan melalui observasi langsung dan kuesioner yang diisi oleh peserta sebelum dan sesudah program pelatihan. Hasil analisis menunjukkan bahwa kesadaran masyarakat meningkat yang dilihat dari hasil tes yang dilakukan menunjukkan peningkatan nilai dari materi yang di ujikan. Pendekatan yang tepat berhasil membangkitkan semangat dan komitmen warga yang tercermin dari tingginya tingkat partisipasi. Pelaksanaan kegiatan ini diharapkan memberikan pengetahuan dan kesadaran masyarakat dalam menjaga kebersihan lingkungan dan pengolahan air bersih.
Column Study and Desorption Performance of Coffee Biomass-Calcium-Alginate Beads to Remove Pb²⁺ Ions from Aqueous Solutions Hanifah, Sarina; Rusnadi, Rusnadi; Stiawan, Elva; Widianti, Riskia Chandra
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.01

Abstract

Heavy metal pollution, particularly by lead (Pb²⁺), poses a serious environmental threat and can significantly impact human health. This study aims to evaluate the performance of an adsorbent composed of a mixture of coffee waste and calcium alginate (Coffee-Calcium-Alginate/CCA beads) in removing Pb²⁺ ions through column experiments, as well as to assess the desorption efficiency and reusability of the adsorbent. Column studies were conducted at 1 ppm Pb2+ ions initial concentration, a flow rate of 2.2 mL/min, using a column with a length of 10 cm and a diameter of 7 mm to determine the breakthrough time of the adsorbate, while desorption was performed using HNO₃ to evaluate the efficiency of Pb²⁺ ions release and the potential for adsorbent reuse. The results demonstrated that the CCA adsorbent was able to retain high efficiency over two adsorption–desorption cycles, with desorption efficiencies of 96.35% and 86.64%. The desorption mechanism involves protonation of carboxylate groups and competition between H⁺ and Pb²⁺ ions at the active sites. Overall, CCA beads exhibit promising potential as an effective and reusable adsorbent for treating water contaminated with heavy metals. Future research could explore the effects of varying initial Pb²⁺ ions concentrations and column height to gain a more comprehensive understanding of desorption dynamics within the column system. Keywords: adsorption, coffee biomass, calcium alginate, Pb2+, leads
Biomass Contents Of Chlorella Pyrenoidosa Cultivated In Cobalt-Less Fertilizer-Enriched Saline Water Widyatama, Awang; Widyatama, Satria Aqilla; Fajry, Zaidan Al; Atmadi, Atmadi; Mulyawati, Ade Heri; Stiawan, Elva
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol. 12 No. 1 (2025): June 2025
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v12i1.39468

Abstract

This research aims to provide some information regarding the metabolite contents of the green microalgae Chlorella pyrenoidosa cultivated in designed saline water enriched with affordable commercial fertilizer products in Indonesia. Chlorella pyrenoidosa growth conditions and bioactive compounds were obtained through cultivation and extraction processes. The microalga growth in two different conditions   was analyzed based on growth curves between microalgae cultivated in a Guillard f/2 medium   and the Cobalt-less fertilizer-enriched saline water.   The biomass was profiled using UPLC-QToF-MS and DPPH assay. As a result, Chlorella pyrenoidosa cultures were normally grown on fertilizer-enriched saline water, but with a lower yield performance than those cultivated in the Guillard f/2 medium. The UPLC-QToF-MS result implies that several peptide and lipid derivatives were detected in the harvested microalgal biomass, with the peptide derivatives as the dominant compounds. The limited antioxidant performance was displayed by the untreated biomass extract. This finding implies that several optimizations are still required in the upstream and downstream stages in culturing Chlorella pyrenoidosa by using an uncommon growth medium.