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Antioxidant Gel from Brown Algae (Ascophyllum nodosum) and Binahong Leaves for Diabetic Wound Healing Rahmadianti, Salma; Sofianty, Irine; Aslamiah, Lisa; Insan, Febrian Khoirul; Sihombing, Maria Engzelita; Nuraini, Vidia Afina; Hernawati, Hernawati
Althea Medical Journal Vol 10, No 4 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n4.3040

Abstract

Background: Diabetic wounds are difficult to treat, causing persistent infections and often leading to limb amputation. One convenient way to manage diabetic wounds is by applying wound dressings. This study aimed to develop a wound dressing in form of a gel containing brown algae and binahong leaf extract for diabetic wound dressings.Methods:  This study was an experimental study conducted in the Organic Chemistry Laboratory of Universitas Pendidikan Indonesia from June to September 2022. As many as 33 mice induced by alloxan were divided into 9 groups consisting of application of brown algae extract gel, binahong lead extract gel and a mixture of brown algae, and binahong leaf extract gel with the concentration of 2.5%, 5%, and 10% respectively. All treatments were given within 7 days. Laboratory data collected included phytochemical screening, total phenolic compound, antioxidant activity and organoleptic test.Results: Brown algae and binahong leaf extracts had a high total phenolic content of 331.25 mg GAE/g and 207.01 mg GAE/g, respectively with antioxidant activity of IC50 = 327.33 µg/mL and 209.30 µg/mL, respectively. It was found that brown algae and binahong leaf extracts could accelerate wound closure in a diabetic mouse model. Gel formulation with 10% w/w brown algae extracts presented 91.66% of wound closure, two times greater than treatment with commercial drugs.Conclusion:  The developed gel containing brown algae and binahong leaf extracts can be a promising wound dressing for healing diabetic wounds.
PENGARUH LAMA FERMENTASI TERHADAP MUTU ORGANOLEPTIK PADA PROSES PEMBUATAN KOMBUCHA SARI KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) Gumanti, Zarfani; Amalia Putri Salsabila; Sihombing, Maria Engzelita; Peristiwati; Kusnadi
Jurnal Pengolahan Pangan Vol 8 No 1 (2023)
Publisher : Fakultas Pertanian Universitas Alkhairaat Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/pangan.v8i1.96

Abstract

Kombucha merupakan produk minuman hasil fermentasi larutan teh dan gula dengan menggunakan starter kultur mikroorganisme kombucha (Acetobacter xylinum dan Saccharomyces cereviseae serta beberapa jenis khamir lainnya) yang difermentasi selama 1-2 minggu. Penelitian ini dilakukan untuk mengetahui pengaruh lama fermentasi terhadap penilaian organoleptik scoresheet berupa warna, aroma, dan rasa. Metode yang digunakan dalam penelitian ini adalah metode eksperimental laboratorium untuk menguji pengaruh waktu fermentasi terhadap mutu organoleptik kombucha sari kulit buah dengan yang terdiri dari 3 perlakuan yaitu lama fermentasi 10 hari, 12 hari, dan 14 hari. Hasil penelitian menunjukkan bahwa lama fermentasi memberikan pengaruh terhadap rasa, aroma dan warna (metode hedonik dan skoring). Lama fermentasi 14 hari direkomendasi sebagai perlakuan terbaik untuk menghasilkan kombucha sari kulit buah naga merah, karena memiliki mutu organoleptik terbaik meliputi rasa manis tidak pekat, aroma asam khas kombucha, dan warna coklat yang jernih dengan kadar pH 3.
The Role of Chloroplast DNA Markers (psbA) in Maintaining the Position of Lemnoideae Sururi, Zaki Fahreza; Nururrahmani, Azmah; Sihombing, Maria Engzelita; Hidayat, Topik
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8906

Abstract

The duckweed plant group belongs to the botanical family Araceae and has the potential as a food source. It is difficult to classify and the plants were originally grouped as the Lemnaceae family. However, current molecular taxonomy studies revealed the integration of Lemnaceae into the Araceae family, thus becoming the Lemnoideae subfamily. It is necessary to strengthen the position of Lemnoideae in Araceae using molecular marker psbA from chloroplast DNA This study aimed to determine the role of the psbA chloroplast DNA marker in regulating the position of Lemnoideae. A total of 41 sequences of the psbA gene taken from the species from seven subfamilies in Araceae and one outgroup were collected from the NCBI GenBank and then arranged in FASTA format. Sequence data was then aligned by ClustalX, and phylogenetic trees were reconstructed using PAUP and MEGA. From the resulting phylogenetic trees, it can be conferred that the Lemnoideae subfamily does not form a monophyletic group. Thereby, this in silico study using psbA markers concludes the position ofthe Lemnoideae subfamily in the family Araceae and we recommend not separating the plant group from the family Araceae.