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Effectivity of Silver Nanoparticles-Temu Giring (Curcuma heyneana) Rhizome on Inhibiting the Growth of Bacteria Causing Nosocomial Infection Nurjamil, Aris Muhamad; Ekajaya, Renandy Kristianlie; Saputro, William Junino; Sururi, Zaki Fahreza; Kusumawaty, Diah
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.284-292

Biofilms are a common cause of nosocomial infections that often attack hospitalized patients. The main objective of this study was to examine the efficacy of silver nanoparticles-temu giring rhizomes in combating bacteria and preventing biofilm formation. The antibacterial and antibiofilm properties of these nanoparticles were evaluated against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The research began with the extraction of temu giring rhizome, synthesis of silver nanoparticles-temu giring rhizome, disk diffusion test, biofilm formation inhibitory activity test, and characterization of silver nanoparticles-temu giring rhizome. In this research, silver nanoparticles-temu giring rhizome were utilized at concentrations of 10, 20, 40, 80, 160 μg/ml, and a control in the form of chlorhexidine. The results showed that the silver nanoparticles-temu giring rhizome produced a larger inhibition zone for bacterial growth compared to the control against the three bacteria. The IC50 value of silver nanoparticles-temu giring rhizome required to inhibit biofilm formation was 27.64 μg/ml in E. coli, 29.29 μg/ml in P. aeruginosa, and 26.21 μg/ml in S. aureus. In P. aeruginosa, E. coli, and S. aureus, the IC50 for preventing biofilm formation by silver nanoparticles-temu giring rhizome was determined to be 27.64 μg/ml, 29.29 μg/ml, and 26.21 μg/ml, respectively. Evaluation of silver nanoparticles revealed the success of temu giring rhizomes in reducing silver ions. This is shown that silver nanoparticles-temu giring rhizomes can be developed into active ingredients that inhibit the growth of bacteria that cause nosocomial infections.

The Role of Chloroplast DNA Markers (psbA) in Maintaining the Position of Lemnoideae Sururi, Zaki Fahreza; Nururrahmani, Azmah; Sihombing, Maria Engzelita; Hidayat, Topik
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8906

The duckweed plant group belongs to the botanical family Araceae and has the potential as a food source. It is difficult to classify and the plants were originally grouped as the Lemnaceae family. However, current molecular taxonomy studies revealed the integration of Lemnaceae into the Araceae family, thus becoming the Lemnoideae subfamily. It is necessary to strengthen the position of Lemnoideae in Araceae using molecular marker psbA from chloroplast DNA This study aimed to determine the role of the psbA chloroplast DNA marker in regulating the position of Lemnoideae. A total of 41 sequences of the psbA gene taken from the species from seven subfamilies in Araceae and one outgroup were collected from the NCBI GenBank and then arranged in FASTA format. Sequence data was then aligned by ClustalX, and phylogenetic trees were reconstructed using PAUP and MEGA. From the resulting phylogenetic trees, it can be conferred that the Lemnoideae subfamily does not form a monophyletic group. Thereby, this in silico study using psbA markers concludes the position ofthe Lemnoideae subfamily in the family Araceae and we recommend not separating the plant group from the family Araceae.

Metabolite Profile of arabica Coffee Cascara from Typica Cultivar in Bandung with Different Drying Processes Sururi, Zaki Fahreza; Diana, Sariwulan; Kusdianti, Kusdianti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.52756

Arabica coffee bean (Coffea arabica L.) is Indonesia's leading commodity, with Bandung Regency as the largest producer in West Java. The exocarp and mesocarp of coffee fruits known as cascara have potential health benefits as a beverage. Cascara is usually dried by direct sunlight before consumption, but this process is prone to weather disturbances and contamination. The use of dehydrators is more controlled to maintain metabolite content, but there has been no research on cascara from coffee cultivars in Bandung Regency dried by this method. This study aims to obtain the metabolite profile of Typica arabica coffee cultivar cascara dried using direct sun and dehydrator. The cascara samples were taken from Gunung Puntang Coffee Plantation, Bandung Regency, and extracted using maceration method with 70% ethanol p.a. solvent. The metabolite content was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS), which specifically detects volatile metabolites. Non-volatile compounds were not within the scope of this study. The results showed that direct sun-dried cascara had 6 metabolites, while dehydrator-dried had 15 metabolites. Both samples were dominated by caffeine (sun-dried cascara: 46.61%, dehydrator cascara: 24.44%). Five metabolites were found in both samples, with 1 unique metabolite in solar cascara and 10 unique metabolites in dehydrator cascara. This study showed differences in metabolite content in Typica cultivar cascara with different drying methods.

The Role of Chloroplast DNA Markers (psbA) in Maintaining the Position of Lemnoideae Sururi, Zaki Fahreza; Nururrahmani, Azmah; Sihombing, Maria Engzelita; Hidayat, Topik
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8906

The duckweed plant group belongs to the botanical family Araceae and has the potential as a food source. It is difficult to classify and the plants were originally grouped as the Lemnaceae family. However, current molecular taxonomy studies revealed the integration of Lemnaceae into the Araceae family, thus becoming the Lemnoideae subfamily. It is necessary to strengthen the position of Lemnoideae in Araceae using molecular marker psbA from chloroplast DNA This study aimed to determine the role of the psbA chloroplast DNA marker in regulating the position of Lemnoideae. A total of 41 sequences of the psbA gene taken from the species from seven subfamilies in Araceae and one outgroup were collected from the NCBI GenBank and then arranged in FASTA format. Sequence data was then aligned by ClustalX, and phylogenetic trees were reconstructed using PAUP and MEGA. From the resulting phylogenetic trees, it can be conferred that the Lemnoideae subfamily does not form a monophyletic group. Thereby, this in silico study using psbA markers concludes the position ofthe Lemnoideae subfamily in the family Araceae and we recommend not separating the plant group from the family Araceae.

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