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All Journal Biogenesis: Jurnal Ilmiah Biologi Jurnal Pendidikan Biologi Indonesia International Journal of Applied Biology Jurnal Kesehatan Manarang Bubungan Tinggi: Jurnal Pengabdian Masyarakat Carmin: Journal of Community Service
Satya Darmayani
Jurusan Analis Kesehatan, Politeknik Kesehatan Kementerian Kesehatan Kendari
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Health Professions Immunology & microbiology Nursing Public Health Social Sciences Other

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The use of silica sand, zeolite and active charcoal to reduce BOD, COD, and TSS of laundry waste water as a biology learning resources Assiddieq, Moch; Darmayani, Satya; Kudonowarso, Wirapati
Jurnal Pendidikan Biologi Indonesia Vol 3, No 3 (2017): NOVEMBER
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (639.382 KB) | DOI: 10.22219/jpbi.v3i3.4864

Abstract

The aquatic environment contaminated with detergent waste in high concentrations can endanger the lives human and biota in the water. This study aimed to determine the decrease of BOD, COD and TSS content of laundry liquid waste by using filter media of silica sand, zeolite, and activated charcoal. Filter medium 1a can reduce BOD level from 98.6 mg/L to 58.80 mg/L (40.36%), while medium 1b can decrease BOD level to 21.20 mg/L (78.48%). It showed that media 1a had not fulfilled the quality standard of BOD of waste water that is 50 mg/L, while media 1b had fulfilled the standard of quality. Filter media 1a can decrease COD level from 210 mg/L to 78.80 mg/L (62.47%), whereas medium 1b can decrease COD to 25.60 mg/L (87.80%). This showed that media 1a and media 1b had met the quality standard of COD of waste water that is 100 mg/L. Filter media 1a can decrease TSS level from 465 mg/L to 122.5 mg/L (73.65%), whereas medium 1b can decrease TSS level to 52.3 mg/L (88.75%). This showed that media 1a and media 1b had met the quality standard of TSS of waste water that is 150 mg/L.
Comparison the number of bacteria between washing hands using soap and hand sanitizer as a bacteriology learning resource for students Darmayani, Satya; Askrening, Askrening; Ariyani, Apita
Jurnal Pendidikan Biologi Indonesia Vol 3, No 3 (2017): NOVEMBER
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.358 KB) | DOI: 10.22219/jpbi.v3i3.4862

Abstract

Hands are the principal carriers of bacterial diseases, therefore very important to know that washing hands with soap or hand sanitizer is highly effective healthy behaviors to reduce bacteria in the palm. This study aimed to determine the total number of bacteria between washing hands with soap and hand sanitizer, also applying the results of these studies as a learning resource in bacteriology. The research design was the true experiment with pretest-posttest control group research design and laboratory examination. Analysis of data using paired t-test and independent sample t-test with α = 0.05. The result using paired t-test obtained t count= 2.48921> t 0.05 (14) = 2.14479 (with liquid soap), obtained t count= 2.32937> t 0.05 (14) = 2.14479 (with hand sanitizer). As for the comparison of the total number of bacteria include washing hands with soap and hand sanitizer using independent samples t-test obtained results there were differences in the total number of bacteria include washing hands with liquid soap and hand sanitizer with t count= 2.23755> t 0.05 ( 13) = 2.16037. That results showed hand sanitizer more effective to reduce the number of bacteria than the liquid soap, that was hand sanitizer 96% and liquid soap by 95%.
Identifikasi Bakteri Salmonella sp. Pada Telur yang dijual di Pasar Kota Kendari Provinsi Sulawesi Tenggara Satya Darmayani; Anita Rosanty; Vina Vanduwinata
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3429

Abstract

This study aimed to identify the presence of Salmonella sp. in eggs sold in the market of Kendari. The World Health Organization (WHO) declared Salmonella sp. is a genus of bacteria that is a major cause of food borne illness in the entire world. Two factors that cause Salmonella sp. contained in eggs are internal and external factors that cause diarrhea or gastroenteritis. The sampling technique was done by purposive sampling, the population is the seller of eggs located in the market of Kendari city that is as much as 172 egg sellers with the sample size are 34 egg samples of chicken. In the process of planting samples on Brain Heart Infusion Broth (BHIB) media, all positive samples were cloudy, indicating bacterial growth in the sample. Furthermore, bacteria were inoculated in Salmonella Shigella Agar (SSA) medium which obtained negative results from 34 samples, no Salmonella sp. colony growth on SSA medium. So it can be concluded, the sample of chicken eggs in this study declared not contaminated by Salmonella sp.
Comparison the number of bacteria between washing hands using soap and hand sanitizer as a bacteriology learning resource for students Satya Darmayani; Askrening Askrening; Apita Ariyani
JPBI (Jurnal Pendidikan Biologi Indonesia) Vol. 3 No. 3 (2017): NOVEMBER
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jpbi.v3i3.4862

Abstract

Hands are the principal carriers of bacterial diseases, therefore very important to know that washing hands with soap or hand sanitizer is highly effective healthy behaviors to reduce bacteria in the palm. This study aimed to determine the total number of bacteria between washing hands with soap and hand sanitizer, also applying the results of these studies as a learning resource in bacteriology. The research design was the true experiment with pretest-posttest control group research design and laboratory examination. Analysis of data using paired t-test and independent sample t-test with α = 0.05. The result using paired t-test obtained t count= 2.48921> t 0.05 (14) = 2.14479 (with liquid soap), obtained t count= 2.32937> t 0.05 (14) = 2.14479 (with hand sanitizer). As for the comparison of the total number of bacteria include washing hands with soap and hand sanitizer using independent samples t-test obtained results there were differences in the total number of bacteria include washing hands with liquid soap and hand sanitizer with t count= 2.23755> t 0.05 ( 13) = 2.16037. That results showed hand sanitizer more effective to reduce the number of bacteria than the liquid soap, that was hand sanitizer 96% and liquid soap by 95%.
The use of silica sand, zeolite and active charcoal to reduce BOD, COD, and TSS of laundry waste water as a biology learning resources Moch Assiddieq; Satya Darmayani; Wirapati Kudonowarso
JPBI (Jurnal Pendidikan Biologi Indonesia) Vol. 3 No. 3 (2017): NOVEMBER
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jpbi.v3i3.4864

Abstract

The aquatic environment contaminated with detergent waste in high concentrations can endanger the lives human and biota in the water. This study aimed to determine the decrease of BOD, COD and TSS content of laundry liquid waste by using filter media of silica sand, zeolite, and activated charcoal. Filter medium 1a can reduce BOD level from 98.6 mg/L to 58.80 mg/L (40.36%), while medium 1b can decrease BOD level to 21.20 mg/L (78.48%). It showed that media 1a had not fulfilled the quality standard of BOD of waste water that is 50 mg/L, while media 1b had fulfilled the standard of quality. Filter media 1a can decrease COD level from 210 mg/L to 78.80 mg/L (62.47%), whereas medium 1b can decrease COD to 25.60 mg/L (87.80%). This showed that media 1a and media 1b had met the quality standard of COD of waste water that is 100 mg/L. Filter media 1a can decrease TSS level from 465 mg/L to 122.5 mg/L (73.65%), whereas medium 1b can decrease TSS level to 52.3 mg/L (88.75%). This showed that media 1a and media 1b had met the quality standard of TSS of waste water that is 150 mg/L.
Inhibition of Juice Komba-Komba (Chromolaenaodorata) Leaf Against Growth of Bacteria Staphylococcus aureus Reni Yunus; Muhammad Hilam; Satya Darmayani
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.6062

Abstract

Nowadays many plants can be used as traditional medicines to overcome various diseases including infections, because the use of traditional medicines is relatively safer compared to drugs derived from chemicals. One of the local plants in Southeast Sulawesi that has been empirically used as a medicine for wounds is a race of plants. Komba-komba leaves (Chromolaena odorata) containcompounds alkaloid, flavonoids, tannins, phenols, triterpenoids, saponins and steroids which have their respective functions in inhibiting the growth of microorganisms or antibacterial. This study aims to determine the inhibition of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus. This type of research is experimental laboratories. The method used was agar diffusion with 5 treatment concentrations, namely komba-sheep leaf extract concentration (Chromolaena odorata) 20%, 40%, 60%, 80% and 100%, positive control (tetracycline) and negative control (aquadest) and repetition . The results showed that there was a inhibition zone of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus at a concentration of 20% at 2 mm, a concentration of 40% at 3.7 mm, a concentration of 60% at 4.7 mm, a concentration of 80% 6.2 mm and a concentration of 100% by 8 mm.
ANALISIS KANDUNGAN BORAKS PADA BAKSO YANG DIJUAL DI ANDUONOHU KOTA KENDARI SULAWESI TENGGARA Sitti Rachmi Misbah; Satya Darmayani; Narti Nasir
Jurnal Kesehatan Manarang Vol 3 No 2 (2017): Desember 2017
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.109 KB) | DOI: 10.33490/jkm.v3i2.41

Abstract

Fast food consumption today has become a habit of eating for the people in Indonesia. One of the popular fast food is meatballs, but nowadays often found the use of non-food additives in the meatballs, namely borax. The Indonesian Republic Food and Drug Administration has detected the abusive use of borax on food derived from various regions of Indonesia. Borax is a chemical compound derived from heavy metals, namely Boron and commonly used as an anti-fungal and wood preservative. This study aimed to determine the content of borax on meatballs that was sold in Anduonohu, Kendari City of Southeast Sulawesi. Type of research using total sampling conducted by observational descriptive by the qualitative method that was using the method of centrifugation and dry ash processing. The results showed that from 24 samples of meatballs located in Anduonohu, Kendari City, it was proven that none of the meatballs contained borax which could endanger the consumer's health which was marked by no change of green color when heated on the flame. Based on the results of the analysis can be concluded that all sellers of meatballs located in Anduonohu, Kendari City have the knowledge and good attitude. Knowledge and attitude level is one important factor in order to avoid pollution of toxin material. Meatball processing method in Anduonohu, Kendari City has fulfilled the requirement of Indonesian Republic Health Minister Regulation in 2015, Number: 722/Menkes/Per /IX/2015 about food, so people do not have to worry about consuming meatballs located in Anduonohu, Kendari City.
Pemberdayaan Masyarakat Pesisir Melalui Pelatihan Pembuatan Sabun dari Limbah Minyak Jelantah & Sereh Berbasis Teknologi Ramah lingkungan (Studi Kasus Masyarakat Pesisir Desa Leppe) Satya Darmayani; Tirta Yoga; Lina Indrawati; Nyimas Yanqoritha; Pahenra Pahenra
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 5, No 1 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v5i1.7633

Abstract

Kandungan senyawa kimia minyak jelantah memiliki sifat karsinogenik, terbentuk ketika proses penggorengan. Konsumsi minyak jelantah secara kontinu bisa mengganggu kesehatan. Namun apabila minyak jelantah dibuang begitu saja dapat mencemari lingkungan. Minyak jelantah bisa bermanfaat, salah satunya diolah menjadi produk sabun. Tujuan kegiatan pengabdian kepada masyarakat ini adalah untuk memberikan pelatihan dengan  mereduksi buangan limbah minyak jelantah ke lingkungan oleh masyarakat pesisir Desa Leppe sehingga resiko pencemaran air tanah bisa dikurangi. Metode yang digunakan yaitu sistem pengolahan limbah untuk mereduksi kedua limbah ini menjadi zero waste industry. Kegiatan ini dilaksanakan pada tanggal 15 November 2022. Sasaran kegiatan ini adalah 50 orang masyarakat Desa Leppe Kec. Soropia. Pengabdian masyarakat ini melalui 3 tahapan yaitu observasi, penyuluhan & pelatihan, serta pemantauan kegiatan evaluasi. Penyuluhan mengenai dampak pembuangan minyak jelantah ke lingkungan yang dilanjutkan dengan praktek membuat sabun dari limbah minyak jelantah serta sereh berbasis teknologi ramah lingkungan. Penyuluhan diawali dengan pemberian pre-test kepada subjek. Penyuluhan diakhiri dengan post-test untuk melihat kedalaman pemahaman subjek target. Indikator pemahaman dievaluasi dari instrumen angket sebelum serta setelah penyuluhan. Trampilnya peserta terlihat dari hasil desain kemasan produk sabun. Mitra yang terlibat pada aktivitas ini yaitu masyarakat pesisir Desa Leppe. Target dari aktivitas pengabdian masyarakat ini yaitu 50 orang masyarakat di Desa Leppe Kecamatan Soropia Kabupaten Konawe yang terdiri atas 6 RT dan 3 Dusun. Dapat disimpulkan bahwa kegiatan pengabdian ini berhasil, terlihat dari kehadiran masyarakat Desa Leppe, yang mengikuti kegiatan ini berjumlah 50 orang. Sehingga memenuhi sasaran total msyarakat yakni 100% dari yang ditargetkan, yakni: Dusun I (17 orang), Dusun II (17 orang), dan Dusun III (16 orang).The chemical compounds in used cooking oil have carcinogenic properties, which are formed during the frying process. Continuous consumption of used cooking oil can interfere with health. However, if used cooking oil is thrown away, it can pollute the environment. Used cooking oil can be helpful, one of which is processed into soap products. The purpose of this community service activity is to provide training by reducing the discharge of used cooking oil waste into the environment by the coastal community of Leppe Village so that the risk of groundwater pollution can be reduced. The method used is a waste treatment system to reduce these two wastes into a zero-waste industry. This activity was carried out on November 15, 2022. The target of this activity was 50 people from Leppe Village, Kec. Soropia. This community service goes through 3 stages: observation, counselling & training, and monitoring of evaluation activities. Counselling on the impact of disposal of used cooking oil into the environment, followed by making soap from waste using cooking oil and citronella based on environmentally friendly technology. Counselling begins with giving a pre-test to the subject. The counselling ended with a post-test to see the depth of understanding of the target subject. Understanding indicators were evaluated from the questionnaire instrument before and after counselling. The soap product packaging design results show the participants' skills. The partners involved in this activity are the coastal communities of Leppe Village. This community service activity targets 50 people in Leppe Village, Soropia District, Konawe Regency, which consists of 6 RTs and 3 Hamlets. It can be concluded that this service activity was successful, can be seen from the presence of the Leppe Village community, who participated in this activity totalling 50 people. To meet the total community target of 100% of the target, namely: Hamlet I (17 people), Hamlet II (17 people), and Hamlet III (16 people). 
Training on Making Hand Sanitizers Made from Local Plants to Prevent the Spread of Covid-19 (Case Study in Kambu District Kendari City) Satya Darmayani; Bestfy Anitasari; Sutiharni Sutiharni; Resy Nirawati; Marni Br Karo
Carmin: Journal of Community Service Vol. 3 No. 1 (2023)
Publisher : Borneo Research and Educatin Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.211 KB) | DOI: 10.59329/carmin.v3i1.52

Abstract

This activity is expected to be a solution to prevent the spread of Corona Virus Disease (Covid-19), which is becoming a global pandemic. This effort is expected to provide additional knowledge to the community about how to make, function, and component, the practice of making and using hand sanitizers made from natural non-alcoholic, and non-toxic ingredients from local plants that are easy to obtain. This activity was carried out in September 2022. Counseling begins with the presentation of material through interactive presentations and discussions with the target subject. Then the counseling continued with the practice of making and using hand sanitizers made from local plants: lime, mint leaves, and aloe vera gel. The activity's success can be said to be successful because it is seen from the participation of the target audience, namely 75% of the targeted minimum of 60%, and the implementation of all community service procedures thoroughly and adequately by the implementing committee of community service activities.
PERBEDAAN HASIL PEMERIKSAAN JUMLAH LEUKOSIT ANTARA METODE MANUAL IMPROVED NEUBAUER DENGAN METODE AUTOMATIC HEMATOLOGY ANALYZER Satya Darmayani; Fonnie Ester Hasan; Devi Ekafitria A.
Jurnal Kesehatan Manarang Vol 2 No 2 (2016): Desember 2016
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v2i2.18

Abstract

Leucocytes are nucleated cells in the blood that can be divided into 5 types. Every cell can be calculated its percentage in the blood by doing types calculation and can be distinguished by the size of the core form, color of granules and cytoplasm inside. This study aimed to determine the results of the difference calculation of leukocytes count examination between Manual Method (Improved Neubauer) and Automatic Hematology Analyzer Method in outpatients at the General Hospital of Kendari. Samples of this study were 30 people taken by accidental sampling. The results of the study with 30 samples using manual method (Improved Neubauer), 25 patients (83.4%) had normal leukocyte and 5 patients (16.6%) had abnormal, and examination using Automatic Hematology Analyzer Method showed 22 patients (73.4%) had normal leukocyte and 8 patients (26.6%) had abnormal. Based on paired test of samples obtained p value = 0, 000, so it can be concluded that there is significant difference between count examination of leukocytes using Manual Method with Improved Neubauer and Automatic Hematology Analyzer Method.
Co-Authors Anita Rosanty Apita Ariyani Ariyani, Apita Askrening Assiddieq, Moch Bestfy Anitasari Devi Ekafitria A. Fonnie Ester Hasan Kudonowarso, Wirapati Lina Indrawati Marni Br Karo Moch Assiddieq Muhammad Hilam Narti Nasir Nyimas Yanqoritha Pahenra Pahenra Reni Yunus, Reni Resy Nirawati Sitti Rachmi Misbah Sutiharni Tirta Yoga Vina Vanduwinata Wirapati Kudonowarso