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Vernandes Sasadara, Maria Malida
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Agriculture, Biological Sciences & Forestry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Uji Aktivitas Antibakteri Ekstrak Etanol Bulung Sangu (Gracilaria Sp.) Terhadap Bakteri Gram Negatif Pseudomonas aeruginosa Putri, Leoni; Vernandes Sasadara, Maria Malida; Cahyaningsih, Erna; Santoso, Puguh
Usadha Vol 2 No 3 (2023): Usadha: Jurnal Integrasi Obat Tradisional
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/usadha.v2i3.7262

Abstract

Pseudomonas aeruginosa is a gram-negative bacterium that is pathogenic to humans, especially in causing nosocomial infections. This bacterium has resistance to several classes of antibiotics, one of which is carbapenem and cephalosporin class antibiotics. The development of new antibiotics is an alternative in solving this case. Seaweed is one of the marine biota that has various benefits to be developed due to its primary and secondary metabolite compounds. Secondary metabolites in seaweed are used as a source of bioactive metabolites which have broad antiviral, antifungal and antibacterial activities. Bulung sangu (Gracilaria sp.) is one of the seaweeds that has biological activity as an antibacterial. This study aims to determine the antibacterial effectiveness of 70% ethanol extract of bulung sangu (Gracilaria sp.) on the growth of Pseudomonas aeruginosa bacteria. Sangu bamboo shoots were obtained from the southern Denpasar area (Bali). Bulung sangu viscous extract was extracted using 70% ethanol by ultrasonic method for 5 minutes at 40oC. Phytochemical screening of the extract was carried out to determine the content of secondary metabolite compounds it had. Antibacterial activity testing was carried out using the well diffusion method using 3 extract concentrations namely 4%, 8% and 12%. The positive and negative controls used were chloramphenicol antibiotics and sterile aquadest. Inhibition zones were observed and expressed as mean ± standard deviation (mm). The results were statistically analyzed using Kruskal-Wallis with a 95% confidence level. The inhibition zones generated from the positive control, concentrations of 4%, 8% and 12% could inhibit Pseudomonas aeruginosa bacteria with the following average diameters of the inhibition zones of 25.67mm, 2.00mm, 2.67mm and 2.67mm in the weak inhibition zone category.
Kajian Etnofarmasi Kelompok Masyarakat Banjar Subamia Kelong, Tabanan Bali Perayanthi, Reni; Anita Dewi, Ni Luh Kade Arman; Kusuma Yuda, Putu Era Sandhi; Cahyaningsih, Erna; Vernandes Sasadara, Maria Malida; Suradnyana, I Gede Made; Sugiyanto, Milyadi
Usadha Vol 3 No 2 (2024): Usadha: Jurnal Integrasi Obat Tradisional
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/usadha.v3i2.7369

Abstract

  Bali is rich in culture and traditions passed down from generation to generation, especially in the ethnopharmacy aspect as a traditional medecine. This research aims to study ethnopharmacy aspects of the application of herbal plants as traditional medicine including plant parts, processing methods, indications, and usage of herbal plants in Banjar Subamia Kelong, Tabanan, Bali. This research was carried out using survey methods, direct observation in the field, and interviews. This research identified 20 types of plants that are generally used by the community in the Banjar Subamia Kelong. The plant parts generally used are leaves (60%), stems (15%), rhizomes (15%) and fruit (10%). Most of the plants are processed by boiling (60%). These medicinal plants are mostly used orally (65%), and the general indication is to treat joint problems, such as muscle pain and gout (25%).
Potensi Tabir Surya pada Tanaman Herbal: Literature Review Wulaningtyas, Ni Ketut Maha; Kusuwa Wardani, I Gusti Agung Ayu; Vernandes Sasadara, Maria Malida
Usadha Vol 2 No 2 (2023): Usadha: Jurnal Integrasi Obat Tradisional
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/usadha.v2i2.7065

Abstract

Paparan sinar matahari dengan intensitas yang tinggi dan secara berlebihan dapat menimbulkan berbagai efek samping, salah satunya adalah kerusakan pada kulit. Oleh karena itu, diperlukan upaya yang tepat untuk mencegah efek-efek yang merugikan bagi kulit, salah satunya adalah dengan penggunaan tabir surya. Tabir surya memiliki kemampuan dalam melindungi kulit dengan cara menunda eritema yang dinyatakan Sun Protection Factor (SPF). Tabir surya yang bersumber dari bahan alam dapat dijadikan alternatif bagi konsumen yang memiliki kulit sensitif terhadap penggunaan tabir surya dari zat aktif kimia. Literature review ini bertujuan untuk mengetahui bahan alam yang memiliki potensi sebagai tabir surya berdasarkan nilai SPFnya.Pencarian literatur dilakukan melalui database Pubmed, Google Scholar dan Science Direct, dengan menggunakan kata kunci yang sesuai. Artikel yang terpilih adalah artikel yang memenuhi kriteria inklusi, yakni artikel yang bersifat original research dan dipublikasikan maksimal 10 tahun terakhir serta sesuai dengan kata kunci yang telah disusun. Berdasarkan hasil penelitian diketahui bahwa terdapat beberapa tanaman yang memiliki proteksi untuk melindungi kulit terhadap sinar ultraviolet seperti tanaman kecombrang (Etlingera elatior), binahong (Anredera cordifolia), Dengen (Dillenia serrata), Kebiul (Caesalpinia bonduc), alga hijau (Ulva reticulata Forsskal), jeruju (Acanthus Ilicifolius), moringa (Moringa oleifera), Zea mays dan(Persea americana). Potensi tabir surya yang dihasilkan oleh tanaman tersebut disebabkan karena adanya kandungan senyawa polifenol seperti flavonoid dan tannin yang berperan sebagai antioksidan kuat.
Co-Authors Anita Dewi, Ni Luh Kade Arman Erna Cahyaningsih Kusuma Yuda, Putu Era Sandhi Kusuwa Wardani, I Gusti Agung Ayu Perayanthi, Reni puguh santoso, puguh Putri, Leoni Sugiyanto, Milyadi Suradnyana, I Gede Made Wulaningtyas, Ni Ketut Maha