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All Journal Jurnal Laboratorium Khatulistiwa
Dj, Herlinda Djohan
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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HUBUNGAN SEKS BEBAS DENGAN KEJADIAN SIFILIS DI WILAYAH KERJA PUSKEMAS KOM YOS SUDARSO KOTA PONTIANAK Dj, Herlinda Djohan; Sutriswanto, Sutriswanto; Nurhayati, Etiek; Ratika, Meila; Salim, Maulidiyah
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1347

Abstract

Syphilis, also known as "the lion king", is an STI (Sexually Transmitted Infection), a disease caused by infection with the Treponema Pallidum bacteria. It is chronic and chronic. This is a dangerous disease because it can attack all organs of the body. This research was conducted at the Kom Yos Sudarso Pontianak Community Health Center because the Community Health Center is a referral health center for syphilis examinations and the respondents were people who were caught by field officers who allowed free sex.               This study aims to find out who suffers from syphilis among respondents who engage in casual sex, as well as finding out the percentage of syphilis incidents in people who engage in casual sex and to find out the relationship between casual sex and the incidence of syphilis in the work area of the Kom Yos Sudarso Community Health Center, Pontianak City.               This research design uses cross sectional with random sampling technique. The samples used were 123 patient blood samples using the TP Rapid method.               Based on the results of the research conducted, casual sex and the incidence of syphilis were obtained. The results of analysis using the chi square test using Continuity Correctionb obtained a value of p = 0.021 < 0.05, so HA was accepted so that there was a significant relationship between casual sex and the incidence of syphilis in the work area of the Community Health Center. Yos Sudarso, Pontianak City.
Aktifitas Perasan Daun Pucuk Merah (Syzygium oleana) Sebagai Bahan Alami Hand Sanitizer Dj, Herlinda Djohan; Sugito, Sugito; Slamet, Slamet
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1095

Abstract

Indonesia is a country that has a fairly high biodiversity, one of which is red shoots (Syzygium oleana). Red shoots are reported to have strong potential to inhibit bacterial growth, including tannins, saponins, flavonoids, and essential oils. To increase effectiveness in utilizing the potential of red shoots, it is made in a practical and easy-to-use dosage form. One of the effective dosage forms for topical therapy is hand sanitizer. The increasing prevalence of the corona virus has caused a shortage of hand sanitizers. Until now, there is no treatment that can cure Corona virus infection. Therefore, the World Health Organization (WHO) appeals to take preventive measures, one of which is washing hands properly. This  study aimed  to examine the antibacterial potential of red shoots (Syzygium oleana) against the growth of Staphylococcus aureus bacteria using the disc method. The juice is made in the form of a Hand Sanitizer Spay. The antibacterial activity was obtained by measuring the diameter of the inhibition zone after giving 4 variations of the concentration of the juice sample. Positive control used amoxicillin 30 g/disk and negative control used aquadest. The next stage of analysis of the quality of hand sanitizers includes organoleptic tests, pH measurements. The research design used is a quasi-experimental method. This research was started in May-December 2022. Samples in the form of red shoots were made in the form of Hand Sanitizer Spay with various concentrations of 5%, 10%, 15%, and 20% and the average inhibition zone value was 11.8 mm, 12.6 mm, 13.75 mm and 14.4 mm. Based on the results of research and computerized data processing with the Simple Linear Regression test, the value of p = 0.000 (p <0.05) means that there is an effect of the concentration of hand sanitizer squeezed from red shoots on the inhibition of Staphylococcus aureus bacteria.
Daya Hambat Air Perasan Daun Pucuk Merah (Syzygium oleana) Terhadap Pertumbuhan Bakteri Staphylococcus aureus Dj, Herlinda Djohan; Sugito, Sugito; Sutriswanto, Sutriswanto
Jurnal Laboratorium Khatulistiwa Vol 5, No 2 (2022): Mei 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i2.972

Abstract

Indonesia is a country that has a fairly high biodiversity, one of which is red shoots (Syzygium oleana). Red shoots are reported to have strong potential to inhibit the growth of bacteria including Staphylococcus aureus because they contain tannins, saponins, flavonoids, and essential oils. To increase effectiveness in exploiting the potential of red shoot leaf extract, it is made in a practical and easy to use dosage form. One of the effective dosage forms for topical therapy is juice. The parameter of this research is the inhibition of red shoot leaf juice (Syzygium oleana) against the growth of Staphylococcus aureus bacteria. This research is a quasi-experimental research (Quasy Experiment). The sample in this study was red shoots leaf juice with 6 repetitions, the number of samples was 24, diluted from the red shoots leaf extract to 5%, the average inhibition zone was 7,33,33 mm, 10% inhibition zone 8 ,17,33 mm, 15% inhibition zone 9 mm and 20% inhibition zone 11.4 mm, amoxicillin positive control and negative control using aquadest. Based on the statistical results of the Anova test, the value of Sig. 0.000 (p= 0.001 < 0.005) which means that there is an effect of red shoot leaf juice (Syzygium oleana) in inhibiting the growth of Staphylococcus aureus by diffusion method.
PERBEDAAN PENGGUNAAN TABUNG VACUTAINER PLAIN DAN CLOT ACTIVATOR TERHADAP WAKTU PEMERIKSAAN GULA DARAH PUASA DI RUMAH SAKIT SULTAN SYARIF MOHAMAD ALKADRIE Dj, Herlinda Djohan; Yuana Putri, Dyah; Kamila, Laila; Tumpuk, Sri
Jurnal Laboratorium Khatulistiwa Vol 6, No 2 (2023): MEI 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i2.1171

Abstract

Tabung vacutainer plain adalah tabung vakum kaca tidak mengandung zat aditif. Tabung vacutainer clot activator adalah tabung vakum plastik mengandung zat aktivator pembekuan. Perbedaan tabung vacutainer plain dan clot activator terdapat pada proses pembekuan darah. Pada tabung vacutainer plain proses pembekuan darah berlangsung normal 15-30 menit sedangkan  tabung vacutainer clot activator relatif lebih cepat proses pembekuan darahnya. Perbedaan waktu pembekuan darah ini membuat hasil pemeriksaan gula darah puasa relatif berbeda.Tujuan penelitian untuk mengkaji perbedaan penggunaan tabung vacutainer plain dan clot activator terhadap waktu pemeriksaan gula darah puasa. Penelitian ini menggunakan desain penelitian cross sectional dan metode pengambilan sampel consecutive sampling. Sampel yang digunakan dalam penelitian ini adalah pasien poli penyakit dalam dengan diagnosa diabetes mellitus tipe 2 yang melakukan pemeriksaan gula darah puasa.Hasil uji Wilcoxon perbedaan waktu pembekuan darah antara  penggunaan tabung vacutainer plain dan clot activator didapat nilai signifikansi 0,000 (p < 0,05) yang berarti Ha diterima, terdapat perbedaan waktu pembekuan darah antara penggunaan tabung vacutainer plain dan clot activator di Rumah Sakit Sultan Syarif Mohamad Alkadrie. Hasil uji Kendall Tau hubungan waktu pembekuan darah tabung vacutainer plain dengan waktu pemeriksaan gula darah puasa diperoleh nilai signifikansi 0,000 (p < 0,05) berarti Ha diterima, ada hubungan antara waktu pembekuan darah penggunaan  vacutainer plain dengan waktu pemeriksaan gula darah puasa di Rumah Sakit Sultan Syarif Mohamad Alkadrie. Sedangkan pada Hasil uji Kendal Tau pada hubungan antara waktu pembekuan darah tabung vacutainer clot activator dengan waktu pemeriksaan gula darah puasa diperoleh nilai signifikansi 0,000 (p < 0,05) berarti Ha diterima, ada hubungan waktu pembekuan darah penggunaan  vacutainer clot activator terhadap waktu pemeriksaan gula darah puasa di Rumah Sakit Sultan Syarif Mohamad Alkadrie.
UJI AKTIVITAS ANTI BAKTERI SEDIAAN SPRAY DAUN KEMANGI (Ocimum sanctum L.) TERHADAP PERTUMBUHAN staphylococcus epidermdis Dj, Herlinda Djohan; Ratnawati, Gervacia Jenny; Sugito, Sugito; D.M.T, Immaculata
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1207

Abstract

Daun kemangi merupakan salah satu tanaman yang memiliki banyak manfaat salah satunya adalah sebagai anti bakteri. Daun kemangi memiliki senyawa kimia seperti flavonoid, tanin, fenolik, saponin dan minyak atsiri yang dapat berperan sebagai anti bakteri. Sehingga dapat digunakan untuk kesehatan dan  kebersihan kaki, terutama untuk penghilang bau kaki yang disebabkan oleh bakteri. Tujuan dari penelitian ini adalah untuk mengetahui adanya aktivitas anti bakteri pada sediaan spray perasan daun kemangi dalam menghambat pertumbuhan bakteri staphylococcus epidermidis. Metode uji yang digunakan ialah metode difusi cakram. Perasan daun kemangi diformulasikan menjadi spray dengan konsentrasi yang berbeda yaitu 15%, 20% dan 25% setiap konsentrasi dilakukan 9 kali pengulangan sehingga sampel yang digunakan sebanyak 27 sampel. Sediaan spray kemudian di uji evaluasi dan di uji aktivitas anti bakteri terhadap bakteri staphylococcus epidermidis. Hasil penelitian menunjukkan bahwa sediaan spray perasan daun kemangi memiliki aktivitas anti bakteri dalam menghambat pertumbuhan staphylococcus epidermidis dengan luas zona hambat berturut-turut sebesar 9,61 mm (F1=15%) kategori sedang, 10,56 mm (F2=20%) kategori sedang, 12,83 mm (F3=25%) kategori kuat.
Co-Authors D.M.T, Immaculata Kamila, Laila Maulidiyah Salim Nurhayati, Etiek Ratika, Meila Ratnawaty, Gervacia Jenny Slamet . Sugito - Sutriswanto, Sutriswanto Tumpuk, Sri Yuana Putri, Dyah