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All Journal Biology, Medicine, & Natural Product Chemistry Indonesian Journal of Global Health research Narra J Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Kusumawati, R. Lia
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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Role of ACE2 and TMPRSS2 polymorphisms on COVID-19 outcome and disease severity in adult patients: A prospective cohort study in a tertiary hospital, Indonesia Yunita, Rina; Wahyuni, Arlinda S.; Sinaga, Bintang YM.; Yamamoto, Zulham; Soebandrio, Amin; Kusumawati, R. Lia; Sembiring, Rosita J.; Pandia, Pandiaman
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.919

Abstract

Coronavirus disease 2019 (COVID-19) has led to a significant number of infections and deaths worldwide, yet its pathogenesis and severity remain incompletely understood. Angiotensin-converting enzyme 2 (ACE2) and transmembrane protease, serine 2 (TMPRSS2), play crucial roles as receptors and molecules responsible for the virus's entry into host cells, initiating the infection process. Their polymorphisms have been extensively studied in relation to COVID-19 severity. The aim of this study was to examine the association of ACE2 (rs2074192) and TMPRSS2 (rs12329760) polymorphisms with COVID-19 outcome and severity. A prospective cohort study was conducted in 2022 at Haji Adam Malik Hospital, Medan, Indonesia. We randomly recruited hospitalized adult patients with COVID-19, confirmed by real-time polymerase chain reaction (RT-PCR). The baseline demographic data, disease severity, underlying disease, comorbidities, and COVID-19 vaccination status were collected. The single-nucleotide polymorphism (SNP) was assessed using TaqMan SNP genotyping assay, and the levels of TMPRSS2 and ACE2 proteins were measured using enzyme-linked immunosorbent assay (ELISA). A total of 151 COVID-19 patients were recruited and there were significant associations between age and severity with mortality outcomes. The age, kidney and lung diseases, and vaccination status were associated with severity levels. The results showed the CC genotype of ACE2 had the highest proportion, followed by TT and CT genotypes among patients, while CT was the most prevalent genotype, followed by CC and TT for TMPRSS2. This study did not find a significant association between ACE2 and TMPRSS2 genetic variants with disease severity and outcome but highlighted a specific association of TMPRSS2 SNP with mortality within the group. In addition, ACE2 concentration was significant different between mild-moderate and severe-critical COVID-19 groups (p=0.033).
The Association of Age, Parity, and History of Cesarean Section with the Occurrence of Placenta Accreta Spectrum (Pas) at H. Adam Malik Hospital in Medan 2020-2022 Siagian, Beby Febyola; Pasaribu, Hotma Partogi; Kusumawati, R. Lia; Daulay, Rini Savitri
Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI) Vol. 6 No. 1 (2024): Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Publisher : TALENTA Publisher, Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jetromi.v6i1.14935

Abstract

Background: Maternal mortality rate (MMR) is an indicator of the quality of midwifery services in a country. Postpartum hemorrhage is a serious life-threatening complication and the main cause of maternal death especially that caused by placenta accreta spectrum (PAS). This study aims to determine the relationship between age, parity, and history of cesarean section on the incidence of PAS at H. Adam Malik Hospital Medan in 2020-2022.Method: This study was an analytic-observational study with a cross-sectional design. The data used came from the medical records, including information about the population of mothers who gave birth, maternal age at delivery, parity, and previous history of cesarean section. Statistical analysis used univariate and bivariate chi-square tests.Result: The study subjects were 84 people selected by simple random sampling method. The results of the univariate analysis showed the highest frequency of age >32 years, parity 3-4 times, and a history of cesarean section ≥ 2. The results of bivariate analysis showed a significant relationship between age and the incidence of PAS (p=0.016), parity and the incidence of PAS (p<0.001), and a history of cesarean section and the incidence of PAS (p=0.004).Conclusion: There is a relationship between age, parity, and history of cesarean section with the incidence of PAS at H. Adam Malik Hospital Medan in 2020-2022.
Antibacterial Effect of Moringa oleifera Leaves on Staphylococcus aureus Based on Different Concentration and Harvest Time Panjaitan, Jeremia Aris; Kusumawati, R. Lia; Yunanda, Yuki; Lubis, Arvitamuriany Triyanthi
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.351-355

Abstract

This study investigates the antibacterial activity of Moringa oleifera leaf extract against Staphylococcus aureus ATCC 25923, focusing on the effects of harvest time and extract concentration. Leaves were harvested in the morning (08:00–10:00) and afternoon (15:00–17:00), then extracted with 96% ethanol. The antibacterial efficacy was evaluated using the Kirby-Bauer disc diffusion method at 60%, 70%, and 80%. The largest inhibition zone was observed at 80% concentration from the afternoon harvest (10.38 ± 0.43 mm), while the smallest was recorded at 60% from the morning harvest (8.25 ± 0.20 mm). Statistical analysis confirmed a significant concentration‑dependent antibacterial effect (p < 0.001, η² = 88.6%). However, harvest time did not significantly affect inhibition zone size (p = 0.882). These results suggest that M. oleifera is an effective natural antimicrobial agent against S. aureus, with concentration being the key determinant of antibacterial activity. Further research is recommended to explore its potential combined with antibiotics and its full therapeutic applicability.
Risk Factors Influencing the Occurrence of Urinary Tract Infections in Inpatients Ardiana, Ardiana; Warli, Syah Mirsya; Ritarwan, Kiking; Kusumawati, R. Lia; Harahap, Juliandi
Indonesian Journal of Global Health Research Vol 6 No 6 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6i6.4016

Abstract

A urinary tract infection is an infection of the urinary tract both upper and or lower, where the number of bacteria is greater than 10⁵ bacterial colonies per unit per ml in a urine specimen. Several studies have shown that there are risk factors that can cause urinary tract infections such as age, gender, long lying down, and other predisposing factors. Objective: To identify the factors that influence the occurrence of urinary tract infections in H. Adam Malik Hospital Medan. Method: This study was designed cross-sectional approach. 59 patients were applied in this study using consecutive sampling. Data were collected using a questionnaire and analyzed using chi-square and Fisher. Results: the results showed that there was no relationship (p>0.05) among age (p=0.869; OR=0.9), gender (p=0.812; OR=1.1), predisposing factors with diabetes mellitus (p=0.146), human immunodeficiency virus (p=0,286), kidney disease (p=0,386), autoimmune (p=0,641) and there were significant relationship (p< 0.05) among prolonged lying down (p=0.04; OR=3,1) were the most significant factor related to urinary tract infection without urinary catheter. Conclusions: the results showed that there was no relationship among age, gender, or predisposing factors such as diabetes mellitus, human immunodeficiency virus, kidney disease, or autoimmune disease with the incidence of urinary tract infection. Long lying down is a relationship with urinary tract infection.
Identification of Microbiota Causing Bromhidrosis in the Axillary Utama, Putri Wahdiah; Jusuf, Nelva Karmila; Putra, Imam Budi; Ichwan, Muhammad; Kusumawati, R. Lia
Indonesian Journal of Global Health Research Vol 7 No 1 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i1.4194

Abstract

Bromhidrosis is a clinical condition defined by an uncomfortable or unpleasant body odor. Since the apocrine glands are only active during puberty, most bromhidrosis occurs in the apocrine glands in the armpit and occurs when apocrine gland secretions approach the skin's surface, where they undergo various biochemical reactions caused by the presence of microbiota in the axillary skin, resulting in odor in the axilla. This research aims to identify microbiota species in the axilla that can cause bromhidrosis. A laboratory experimental research design with purposive sampling is used in this study. In this study, 60 samples were collected from students aged 15 to 18 years old at a high school in Medan, and the samples were analyzed at the University of North Sumatra Hospital's Microbiology Laboratory. All samples were taken from the right axillary skin swab, a Gram stain was performed, and the identified microbiota isolates were identified. The results of skin smear culture revealed a Gram-positive cocci group in 59 samples (98.3%) and a Gram-positive bacilli group in only one sample (1.7%). The microbiota responsible for bromhidrosis was identified as Staphylococcus hominis spp. hominins (30.4%), Staphylococcus epidermidis (27.8%), Staphylococcus aureus (16.5%), Kocuria kristinae and Staphylococcus haemolyticus (6.3%), Staphylococcus lugdunensis and Staphylococcus saprophyticus. The most common microbiota that causes bromhidrosis in the axilla is Staphylococcis hominis spp. Hominis, Staphylococcus epidermidis, and Staphylococcus aureus.
Co-Authors Amin Soebandrio Ardiana Ardiana, Ardiana Harahap, Juliandi Jusuf, Nelva Karmila Kiking Ritarwan Lubis, Arvitamuriany Triyanthi Muhammad Ichwan Pandia, Pandiaman Panjaitan, Jeremia Aris Pasaribu, Hotma Partogi Putra, Imam Budi Rina Yunita Rini Savitri Daulay, Rini Savitri Sembiring, Rosita J. Siagian, Beby Febyola Sinaga, Bintang YM. Utama, Putri Wahdiah Wahyuni, Arlinda S. Warli, Syah Mirsya Yamamoto, Zulham Yunanda, Yuki