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All Journal Biology, Medicine, & Natural Product Chemistry Narra J Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Kusumawati, R. Lia
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Role of ACE2 and TMPRSS2 polymorphisms on COVID-19 outcome and disease severity in adult patients: A prospective cohort study in a tertiary hospital, Indonesia Yunita, Rina; Wahyuni, Arlinda S.; Sinaga, Bintang YM.; Yamamoto, Zulham; Soebandrio, Amin; Kusumawati, R. Lia; Sembiring, Rosita J.; Pandia, Pandiaman
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.919

Abstract

Coronavirus disease 2019 (COVID-19) has led to a significant number of infections and deaths worldwide, yet its pathogenesis and severity remain incompletely understood. Angiotensin-converting enzyme 2 (ACE2) and transmembrane protease, serine 2 (TMPRSS2), play crucial roles as receptors and molecules responsible for the virus's entry into host cells, initiating the infection process. Their polymorphisms have been extensively studied in relation to COVID-19 severity. The aim of this study was to examine the association of ACE2 (rs2074192) and TMPRSS2 (rs12329760) polymorphisms with COVID-19 outcome and severity. A prospective cohort study was conducted in 2022 at Haji Adam Malik Hospital, Medan, Indonesia. We randomly recruited hospitalized adult patients with COVID-19, confirmed by real-time polymerase chain reaction (RT-PCR). The baseline demographic data, disease severity, underlying disease, comorbidities, and COVID-19 vaccination status were collected. The single-nucleotide polymorphism (SNP) was assessed using TaqMan SNP genotyping assay, and the levels of TMPRSS2 and ACE2 proteins were measured using enzyme-linked immunosorbent assay (ELISA). A total of 151 COVID-19 patients were recruited and there were significant associations between age and severity with mortality outcomes. The age, kidney and lung diseases, and vaccination status were associated with severity levels. The results showed the CC genotype of ACE2 had the highest proportion, followed by TT and CT genotypes among patients, while CT was the most prevalent genotype, followed by CC and TT for TMPRSS2. This study did not find a significant association between ACE2 and TMPRSS2 genetic variants with disease severity and outcome but highlighted a specific association of TMPRSS2 SNP with mortality within the group. In addition, ACE2 concentration was significant different between mild-moderate and severe-critical COVID-19 groups (p=0.033).
The Association of Age, Parity, and History of Cesarean Section with the Occurrence of Placenta Accreta Spectrum (Pas) at H. Adam Malik Hospital in Medan 2020-2022 Siagian, Beby Febyola; Pasaribu, Hotma Partogi; Kusumawati, R. Lia; Daulay, Rini Savitri
Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI) Vol. 6 No. 1 (2024): Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Publisher : TALENTA Publisher, Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jetromi.v6i1.14935

Abstract

Background: Maternal mortality rate (MMR) is an indicator of the quality of midwifery services in a country. Postpartum hemorrhage is a serious life-threatening complication and the main cause of maternal death especially that caused by placenta accreta spectrum (PAS). This study aims to determine the relationship between age, parity, and history of cesarean section on the incidence of PAS at H. Adam Malik Hospital Medan in 2020-2022.Method: This study was an analytic-observational study with a cross-sectional design. The data used came from the medical records, including information about the population of mothers who gave birth, maternal age at delivery, parity, and previous history of cesarean section. Statistical analysis used univariate and bivariate chi-square tests.Result: The study subjects were 84 people selected by simple random sampling method. The results of the univariate analysis showed the highest frequency of age >32 years, parity 3-4 times, and a history of cesarean section ≥ 2. The results of bivariate analysis showed a significant relationship between age and the incidence of PAS (p=0.016), parity and the incidence of PAS (p<0.001), and a history of cesarean section and the incidence of PAS (p=0.004).Conclusion: There is a relationship between age, parity, and history of cesarean section with the incidence of PAS at H. Adam Malik Hospital Medan in 2020-2022.
Antibacterial Effect of Moringa oleifera Leaves on Staphylococcus aureus Based on Different Concentration and Harvest Time Panjaitan, Jeremia Aris; Kusumawati, R. Lia; Yunanda, Yuki; Lubis, Arvitamuriany Triyanthi
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.351-355

Abstract

This study investigates the antibacterial activity of Moringa oleifera leaf extract against Staphylococcus aureus ATCC 25923, focusing on the effects of harvest time and extract concentration. Leaves were harvested in the morning (08:00–10:00) and afternoon (15:00–17:00), then extracted with 96% ethanol. The antibacterial efficacy was evaluated using the Kirby-Bauer disc diffusion method at 60%, 70%, and 80%. The largest inhibition zone was observed at 80% concentration from the afternoon harvest (10.38 ± 0.43 mm), while the smallest was recorded at 60% from the morning harvest (8.25 ± 0.20 mm). Statistical analysis confirmed a significant concentration‑dependent antibacterial effect (p < 0.001, η² = 88.6%). However, harvest time did not significantly affect inhibition zone size (p = 0.882). These results suggest that M. oleifera is an effective natural antimicrobial agent against S. aureus, with concentration being the key determinant of antibacterial activity. Further research is recommended to explore its potential combined with antibiotics and its full therapeutic applicability.
Co-Authors Amin Soebandrio Lubis, Arvitamuriany Triyanthi Pandia, Pandiaman Panjaitan, Jeremia Aris Pasaribu, Hotma Partogi Rina Yunita Rini Savitri Daulay, Rini Savitri Sembiring, Rosita J. Siagian, Beby Febyola Sinaga, Bintang YM. Wahyuni, Arlinda S. Yamamoto, Zulham Yunanda, Yuki