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All Journal Jurnal DiMas (Jurnal Pengabdian Kepada Masyarakat) Media Farmasi Indonesia
Dwi WULANSARI, Endang
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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UJI KANDUNGAN FENOLIK TOTAL DAN AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH ASAM PAYA (Eleiodoxa conferta (Griff.) Burret) TERHADAP Staphylococcus aureus. Elisabeth SITORUS, Febrina Claudia; Dwi WULANSARI, Endang; SULISTYARINI, Indah
Media Farmasi Indonesia Vol. 15 No. 2 (2020): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.003 KB) | DOI: 10.53359/mfi.v15i2.163

Abstract

Asam paya (Eleiodoxa conferta (Griff.) Burret) contains phenolic compounds which can function as antibacterial agents against Staphylococcus aureus. Extraction using soxhlet with multilevel polarity, namely n-hexane and ethanol. The antibacterial activity test by TLC contact-bioautography showed that the phenolic compounds in the ethanol extract had antibacterial activity, and the diffusion test results showed that the ethanol extract had a larger inhibition zone diameter than the n-hexane extract. The total phenolic compound of extract was determined using the Folin-Ciocalteu reagent. The total phenolic compounds of Eleiodoxa conferta (Griff.) Burret. ethanolic extract is 7,326± 0,0989 mg GAE/gram sample. The ethanol extract was fractionated using a separating funnel, followed by purification with preparative TLC. The phenolic isolates obtained were tested for purity by three eluent TLC. The results of antibacterial activity diffution test and TLC contact-bioautography test showed that phenolic isolates had antibacterial activity against Staphylococcus aureus.
PERBANDINGAN KANDUNGAN FENOLIK TOTAL, FLAVONOIDTOTAL DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLDAUN JAMBU BOL (Syzigium malaccense L.) DAN DAUN JAMBU AIR KANCING (Syzigium aqueum) Zahrani Primadiastri, Ilavi; Dwi Wulansari, Endang; Suharsanti, Ririn
Media Farmasi Indonesia Vol. 16 No. 2 (2021): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.028 KB) | DOI: 10.53359/mfi.v16i2.180

Abstract

Leaves of malay apple (Syzygium malaccense L.) and water apple (Syzygium aqueum) are plants that contain phenolic and flavonoid compounds which have antioxidant activity. The aim of this study was to determine the differences in total phenolic content, total flavonoids and antioxidant activity of ethanol extract of malay apple leaves and water apple leaves. The extraction method uses maceration with 96% ethanol solvent in a ratio of 1:15. The ethanol extract of malay apple leaves and water apple was tested for total phenolic content using the Folin-Ciocalteu method, total flavonoids using the colorimetric method and antioxidant activity using the DPPH method. Qualitative identification of quercetin compounds was performed by densitometry tlc. In the phenolic and flavonoid content test, the total sample extract was made with a concentration of 5000 ppm. In the antioxidant activity test of the extract samples, a series of concentrations of 80, 100, 120, 140 and 160 ppm were made. The results showed that the total phenolic content in the ethanol extract of malay apple leaves has an average of 12.5149 mgGAE/g and water apple leaves of 14.3207 mgGAE/g. The total flavonoid content in the ethanol extract of malay apple leaves has an average of 9.1613 mgQE/g and water apple leaves of 16.5893 mgQE/g. The ethanol extract of malay apple leaves has antioxidant activity with an average IC50 of 138.3315 ppm and water apple leaves of 117.6745 ppm. In qualitative identification densitometry the Rf value of quercetin is 0,68; ethanol extract of malay apple leaves is 0,68 and water apple leaves is 0,85. The results of the independent statistical T test showed that there were differences in the total phenolic content, total flavonoids and antioxidant activity in the ethanol extract of malay apple leaves and water apple leaves.
Penyuluhan Bahaya Napza Dan Pembuatan Infused Water Di SMA IT Harapan Bunda Semarang Dwi Wulansari, Endang; Asri Elina, Alifah; Nanta Pratiwi, Anisa; Novita Meiliani, Chandra; Meydina Eka, Fabio; Khairunnisa
Jurnal Dimas Vol 2 No 1 (2020): Jurnal Pengabdian Kepada Masyarakat
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1366.898 KB) | DOI: 10.53359/dimas.v2i1.18

Abstract

NAPZA (Narkotika, Psikotropika dan Zat Adiktif) merupakan zat yang bila masuk ke dalam tubuh dapatmempengaruhi tubuh terutama susunan syaraf pusat otak, sehingga bila disalahgunakan akan menyebabkangangguan fisik, psikis dan fungsi sosial. Salah satu kelompok masyarakat yang rawan terpapar penyalahgunaannarkoba adalah pada rentang umur 15-35 tahun atau generasi milenial. Menurut Badan Narkotika Nasional(BNN), penyalahgunaan NAPZA dikalangan siswa ditahun 2017 mencapai angka 3,38 juta orang. Usia remajamerupakan usia rentan terhadap penyalahgunaan NAPZA karena sikap emosi dan mental mereka yang masihsangat labil sehingga mudah terpengaruh kedalam perilaku menyimpang. Mereka cenderung memiliki rasa ingintahu yang tinggi terhadap narkotika dan psikotropika yang menjadi salah satu pendorong bagi seseorang untukuntuk melakukan perbuatan menyimpang. Penyuluhan bertujuan untuk meningkatkan pengetahuan siswa tentangbahaya yang ditimbulkan dari penyalahgunaan NAPZA sehingga dapat mencegah penggunaan NAPZA dikalangansiswa. Selain itu, diharapkan para siswa mampu membuat infused water kombinasi lemon, strawberry dan daunmint yang dapat digunakan sebagai antioksidan. Kegiatan penyuluhan dilakukan di SMA IT Harapan Bunda,Semarang. Kuesioner digunakan sebagai alat evaluasi untuk mendapatkan umpan balik tentang pelaksanaan danmanfaat kegiatan. Hasil penilaikan siswa terhadap pelaksanaan penyuluhan secara umum dinilai baik dalam halpengelolaan waktu kegiatan, penyaji, materi yang disampaikan, serta manfaat kegiatan.
Co-Authors Asri Elina, Alifah Elisabeth SITORUS, Febrina Claudia Indah Sulistyarini Khairunnisa Meydina Eka, Fabio Nanta Pratiwi, Anisa Novita Meiliani, Chandra Ririn Suharsanti, Ririn Zahrani Primadiastri, Ilavi