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All Journal Journal of Research and Technology Journal of Pharmaceutical Care Anwar Medika Journal Pharmasci (Journal of Pharmacy and Science) Farmasis: Jurnal Sains Farmasi
Sudarwati, Tri Puji Lestari
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

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AKTIVITAS ANTIBAKTERI DAUN PEPAYA (Carica Pepaya) MENGGUNAKAN PELARUT ETANOL TERHADAP BAKTERI Salmonella thypi Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol 4, No 1 (2018)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.043 KB) | DOI: 10.5281/zenodo.2580446

Abstract

Typhoid fever is one of the health problems that often occur in people of Indonesia. The spreads of this desease are through contaminated food. Thyphoid fever could be caused by bacterials infection, one of the bactery is Salmonella typhi. One of solution o cure the infection is by using antibacterial derived from plants or herbs called traditional medicine.  This medicine is chosen and preffered because it has relatively small side effects. Carica papaya L. is the most kind of plant which is learnt for the function as antibacterial. This study aims to determine the concentration effects of papaya leaf extract to the inhibition zone of Salmonella typhi bacteria. The method to determine the concentration effect of papaya leaf extract is a paper disc difussion.  The results of this study at a concentration of 20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml and 100 μg/ml indicate inhibiton zone in the category of moderate.
LAJU PERKEMBANGAN Aedes aegypti PADA BEBERAPA MEDIA YANG BERBEDA Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol 1, No 01 (2015)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.096 KB) | DOI: 10.5281/zenodo.2581122

Abstract

This research was conducted to determine the pace of development of larvae of Aedes aegypti in some media of water, with the aim of knowing the development of Aedes aegypti mosquitoes from egg to be by looking at the development and physiology. The research was conducted between February and April 2008. The material used is the Aedes aegypti mosquito eggs laid on 3 media: water bath water, sewage, and water ponds. The data obtained were statistically analyzed using SPSS. The development of mosquito larvae in the sewer water is 8.3 days, 21.5 days of pool water, water bath of 24.3 days. Results of statistical analysis of all the observed variables showed that, there is a real difference between the sewage and the pool water and bath water, but the pool water and bath water are not significantly different. It can be concluded that the difference in water media affect the development of larvae of Aedes aegypti. Keywords: Aedes aegypti, water bath, water sewer, water ponds.
Aktivitas Antibakteri Daun Pepaya (Carica Pepaya) Menggunakan Pelarut Etanol Terhadap Bakteri Escherichia Coli Sudarwati, Tri Puji Lestari; Suryandari, Mercyska
Journal of Pharmaceutical Care Anwar Medika (J-PhAM) Vol 1 No 2 (2019): Volume 1, Nomor 2, Juni 2019
Publisher : STIKES Rumah Sakit Anwar Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36932/jpcam.v1i2.11

Abstract

Isolasi zat aktif dari herba menimbulkan pandangan baru bahwa tiap herba memiliki zat aktif (satu atau lebih). Carica papaya L memiliki zat aktif yang berhasil diisolasi, zat – zat tersebut dapat menggantikan pemakaian herba untuk tujuan pengobatan. Escherichia coli merupakan bakteri yang banyak terdapat di usus besar (colon) manusia dan sebagai flora normal colon, sifat Escherichia coli dapat menyebabkan infeksi primer pada usus besar sehingga menyebabkan penyakit diare. Sampel yang digunakan adalah Daun Pepaya tua berwarna hijau tua yang diperoleh dari UPT Materia Medika Batu. Dicuci bersih kemudian dikeringkan, kemudian dihaluskan dengan cara diblender. Proses ekstraksi menggunakan metode maserasi. Pengujian dilakukan dengan menggunakan 5 konsentrasi dan kontrol negatif. Metode pengujian antibakteri yang digunakan adalah difusi kertas cakram. Diameter zona hambat pada tiap konsentrasi dianalisis menggunakan statistik menggunakan uji ANOVA. Berdasarkan hasil pengujian dengan melakukan replikasi sebanyak 6 kali pada konsentrasi 20 µ g/mL dengan rata – rata 7,25 mm, 40 µ g/mL dengan rata – rata 7,43 mm, 60µ g/mL dengan rata – rata 7,7 mm, 80 µ g/mL dengan rata – rata 7,8 dan 100 µ g/mL dengan rata – rata 7,9 mm dengan kategori sedang. Dapat dikatakan terdapat pengaruh aktivitas antibakteri pada Daun Pepaya (Carica papaya L) dengan bakteri Escherichia coli maka, semakin tinggi konsentrasi maka daya hambat yang dihasilkan semakin tinggi.
Potensi Antimikroba Ekstrak Ethanol Ganoderma lucidum Menggunakan Metode Bioautografi terhadap Bakteri Escherichia coli dan Bacillus subtillis Sudarwati, Tri Puji Lestari; Fernanda, M.A. Hanny Ferry
Journal of Pharmacy and Science Vol. 6 No. 1 (2021): Journal of Pharmacy and Science
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v6i1.212

Abstract

Potensi antibakteri ekstrak ethanol jamur lingzhi (Ganoderma lucidum) menggunakan metode analisis KLT bioautografi terhadap Escherichia coli dan Bacillus subtillis sebagai penelitian pendahuluan untuk mengetahui kandungan metabolit sekunder pada jamur lingzi. Soxhletasi merupakan metode untuk mengekstraksi serbuk jamur lingzhi menggunakan pelarut etanol. Bioautografi merupakan metode yang digunakan untuk mengetahui kandungan metabolit sekunder pada jamur lingzi dan potensi antibakteri. Konsentrasi yang digunakan yaitu: 2%, 4%, 6%, 8%dan 10%, dengan pengulangan sebanyak 5 kali. KLT bioautografi menggunakan n-butanol : Asam asetat glasial : Air (4:1:5) sebagai eluent. Analisis yang telah dilakukan menghasilkan 3 noda dengan Rf 0,5 dengan warna kuning, Rf 0,87 warna biru, Rf 0,93 warna coklat, dengan daerah hambatan pada noda coklat dengan Rf 0,93 merupakan senyawa triterpen. Hasil dari pengukuran nilai rata-rata zona hambat yang diperoleh terhadap bakteri E.coli adalah sebagai berikut, pada konsentrasi 2% 5,45 mm dengan kategori lemah, konsentrasi 4% 5,14 mm, konsentrasi 6% 5,48 mm, konsentrasi 8% 5,94 mm dan konsentrasi terbesar terdapat pada konsentrasi 10% 6,98 mm dengan kategori sedang. Pada bakteri Bakteri Bacillus subtillis konsentrasi 2% Sebesar 5,9 mm, Konsentrasi 4% Sebesar 6,34 mm, Konsentrasi 6% Sebesar 6,68 mm, Konsentrasi 8% Sebesar 7,88 mm, Dan Konsentrasi 10% Sebesar 8,6 mm dengan Kategori Sedang Maka dapat disimpulkan bahwa ekstrak jamur lingzhi memiliki aktivitas antibakteri terhadap bakteri Escherichia coli. Sehingga semakin besar konsentrasi yang digunakan, semakin besar pula aktivitasnya dalam menghambat pertumbuhan bakteri.
Fraksinasi dan Identifikasi Ektrak Daun Mitragyna Speciosa Menggunakan Metode Kromatografi Fernanda, M.A. Hanny Ferry; Suryandari, Mercyska; Sudarwati, Tri Puji Lestari
FARMASIS: Jurnal Sains Farmasi Vol 2 No 2 (2021): Farmasis : Jurnal Sains Farmasi
Publisher : Universitas PGRI Adi Buana, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/farmasis.v2i2.4072

Abstract

Background: Mitragyna Speciosa or better known as Kratom is a typical plant from the Putusibau area, West Kalimantan. The most widely used part of this plant is the leaves. The kratom plant contains alkaloids, flavonoids, phenols, terpenoids-steroids, triterpenoids, saponins, and tannins. Alkaloid content is the most dominant and has benefits in this plant so it is necessary to do fractionation and identification of secondary metabolites. Objective: To fractionate and identify secondary metabolites from Mitragyna Speciosa leaf extract. Methods: The fractionation method was carried out using Column Chromatography while for identification using Thin Layer Chromatography. Results: 80 fractions were obtained from the column chromatography results, while the results of identification using thin layer chromatography showed that only the 2nd and 3rd fractions were obtained. The second fraction has two spots with Rf values of 0.51 and 0.73 and in the third fraction there are three spots with Rf values of 0.21; 0.52, and 0.74. Conclusion: Fractionation and identification by chromatographic method can be used as initial screening for secondary metabolite content in Mitragyna Speciosa leaf extract.
Concentration and Time Exposure Determination of Methanol Extract from Carica papaya Leaves in The Larvicidal Activity Against Aedes aegypti Larvae Sudarwati, Tri Puji Lestari; M. A Hanny Ferry Fernanda
Journal of Research and Technology Vol. 7 No. 1 (2021): JRT Volume 7 No 1 Jun 2021
Publisher : 2477 - 6165

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55732/jrt.v7i1.288

Abstract

Dengue Haemorrhagic Fever (DHF) is a disease caused by dengue virus which is transmitted through Aedes aegypti mosquito bite. Efforts to control the widespread of the vectors have been made using biological agents and also chemical compound. Chemicals known as a standard protocol have raised concerns about resistance and harmfulness to the environment. Hence, the present study was aimed to explore the larvicidal activity of papaya (Carica papaya) leaf extract against Aedes aegypti larvae in regards to the optimum concentration and time exposure.  Preparation the obtained extract was diluted to make a serial concentration. These solutions were made by pipetting 0.65 mL, 1.25 mL, 2.5 mL, 5.0 mL, and 10.0 mL of extract into 10.0 mL volumetric flasks and dilute with distilled water. The test solution was poured into a glass jar contained 90 mL of distilled water and filled with 20 third instar larvae. Each experiment was replicated four times. The larval mortality was recorded in 24h and calculated as a percentage of total larvae used in the experiment. The table above shows the value of LC50 And LT50 from toxicity assay of papaya leaf extract. According to the LT50 value, it can be seen that the lowest LT50 of 1,006h occurred at the concentration of 11000 ppm. Moreover, calculated LC50 is 4929,344 ppm. Based on these results, papaya leaves have the ability to Aedes aegypti larvaside so that it can help in breaking the chain of development of Aedes aegypti.  
LAJU PERKEMBANGAN Aedes aegypti PADA BEBERAPA MEDIA YANG BERBEDA Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol. 1 No. 1 (2015): JRT Volume 1 No 1 Des 2015
Publisher : 2477 - 6165

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55732/jrt.v1i1.329

Abstract

This research was conducted to determine the pace of development of larvae of Aedes aegypti in some media of water, with the aim of knowing the development of Aedes aegypti mosquitoes from egg to be by looking at the development and physiology. The research was conducted between February and April 2008. The material used is the Aedes aegypti mosquito eggs laid on 3 media: water bath water, sewage, and water ponds. The data obtained were statistically analyzed using SPSS. The development of mosquito larvae in the sewer water is 8.3 days, 21.5 days of pool water, water bath of 24.3 days. Results of statistical analysis of all the observed variables showed that, there is a real difference between the sewage and the pool water and bath water, but the pool water and bath water are not significantly different. It can be concluded that the difference in water media affect the development of larvae of Aedes aegypti.
Concentration and Time Exposure Determination of Methanol Extract from Carica papaya Leaves in The Larvicidal Activity Against Aedes aegypti Larvae Sudarwati, Tri Puji Lestari; M. A Hanny Ferry Fernanda
Journal of Research and Technology Vol. 7 No. 1 (2021): JRT Volume 7 No 1 Jun 2021
Publisher : 2477 - 6165

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55732/jrt.v7i1.288

Abstract

Dengue Haemorrhagic Fever (DHF) is a disease caused by dengue virus which is transmitted through Aedes aegypti mosquito bite. Efforts to control the widespread of the vectors have been made using biological agents and also chemical compound. Chemicals known as a standard protocol have raised concerns about resistance and harmfulness to the environment. Hence, the present study was aimed to explore the larvicidal activity of papaya (Carica papaya) leaf extract against Aedes aegypti larvae in regards to the optimum concentration and time exposure.  Preparation the obtained extract was diluted to make a serial concentration. These solutions were made by pipetting 0.65 mL, 1.25 mL, 2.5 mL, 5.0 mL, and 10.0 mL of extract into 10.0 mL volumetric flasks and dilute with distilled water. The test solution was poured into a glass jar contained 90 mL of distilled water and filled with 20 third instar larvae. Each experiment was replicated four times. The larval mortality was recorded in 24h and calculated as a percentage of total larvae used in the experiment. The table above shows the value of LC50 And LT50 from toxicity assay of papaya leaf extract. According to the LT50 value, it can be seen that the lowest LT50 of 1,006h occurred at the concentration of 11000 ppm. Moreover, calculated LC50 is 4929,344 ppm. Based on these results, papaya leaves have the ability to Aedes aegypti larvaside so that it can help in breaking the chain of development of Aedes aegypti.  
Co-Authors Fernanda, M.A. Hanny Ferry M. A Hanny Ferry Fernanda Suryandari, Mercyska