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All Journal Jurnal Kimia Riset Journal of Research and Technology Journal of Pharmaceutical Care Anwar Medika Journal Pharmasci (Journal of Pharmacy and Science) Farmasis: Jurnal Sains Farmasi Jurnal Abdi Masyarakat Kita Jurnal Komunitas Farmasi Nasional
Tri Puji Lestari Sudarwati
Akademi Farmasi Surabaya
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

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AKTIVITAS ANTIBAKTERI DAUN PEPAYA (Carica Pepaya) MENGGUNAKAN PELARUT ETANOL TERHADAP BAKTERI Salmonella thypi Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol 4, No 1 (2018)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.043 KB) | DOI: 10.5281/zenodo.2580446

Abstract

Typhoid fever is one of the health problems that often occur in people of Indonesia. The spreads of this desease are through contaminated food. Thyphoid fever could be caused by bacterials infection, one of the bactery is Salmonella typhi. One of solution o cure the infection is by using antibacterial derived from plants or herbs called traditional medicine.  This medicine is chosen and preffered because it has relatively small side effects. Carica papaya L. is the most kind of plant which is learnt for the function as antibacterial. This study aims to determine the concentration effects of papaya leaf extract to the inhibition zone of Salmonella typhi bacteria. The method to determine the concentration effect of papaya leaf extract is a paper disc difussion.  The results of this study at a concentration of 20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml and 100 μg/ml indicate inhibiton zone in the category of moderate.
LAJU PERKEMBANGAN Aedes aegypti PADA BEBERAPA MEDIA YANG BERBEDA Sudarwati, Tri Puji Lestari
Journal of Research and Technology Vol 1, No 01 (2015)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.096 KB) | DOI: 10.5281/zenodo.2581122

Abstract

This research was conducted to determine the pace of development of larvae of Aedes aegypti in some media of water, with the aim of knowing the development of Aedes aegypti mosquitoes from egg to be by looking at the development and physiology. The research was conducted between February and April 2008. The material used is the Aedes aegypti mosquito eggs laid on 3 media: water bath water, sewage, and water ponds. The data obtained were statistically analyzed using SPSS. The development of mosquito larvae in the sewer water is 8.3 days, 21.5 days of pool water, water bath of 24.3 days. Results of statistical analysis of all the observed variables showed that, there is a real difference between the sewage and the pool water and bath water, but the pool water and bath water are not significantly different. It can be concluded that the difference in water media affect the development of larvae of Aedes aegypti. Keywords: Aedes aegypti, water bath, water sewer, water ponds.
Aktivitas Antibakteri Daun Pepaya (Carica Pepaya) Menggunakan Pelarut Etanol Terhadap Bakteri Escherichia Coli Sudarwati, Tri Puji Lestari; Suryandari, Mercyska
Journal of Pharmaceutical Care Anwar Medika (J-PhAM) Vol 1 No 2 (2019): Volume 1, Nomor 2, Juni 2019
Publisher : STIKES Rumah Sakit Anwar Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36932/jpcam.v1i2.11

Abstract

Isolasi zat aktif dari herba menimbulkan pandangan baru bahwa tiap herba memiliki zat aktif (satu atau lebih). Carica papaya L memiliki zat aktif yang berhasil diisolasi, zat – zat tersebut dapat menggantikan pemakaian herba untuk tujuan pengobatan. Escherichia coli merupakan bakteri yang banyak terdapat di usus besar (colon) manusia dan sebagai flora normal colon, sifat Escherichia coli dapat menyebabkan infeksi primer pada usus besar sehingga menyebabkan penyakit diare. Sampel yang digunakan adalah Daun Pepaya tua berwarna hijau tua yang diperoleh dari UPT Materia Medika Batu. Dicuci bersih kemudian dikeringkan, kemudian dihaluskan dengan cara diblender. Proses ekstraksi menggunakan metode maserasi. Pengujian dilakukan dengan menggunakan 5 konsentrasi dan kontrol negatif. Metode pengujian antibakteri yang digunakan adalah difusi kertas cakram. Diameter zona hambat pada tiap konsentrasi dianalisis menggunakan statistik menggunakan uji ANOVA. Berdasarkan hasil pengujian dengan melakukan replikasi sebanyak 6 kali pada konsentrasi 20 µ g/mL dengan rata – rata 7,25 mm, 40 µ g/mL dengan rata – rata 7,43 mm, 60µ g/mL dengan rata – rata 7,7 mm, 80 µ g/mL dengan rata – rata 7,8 dan 100 µ g/mL dengan rata – rata 7,9 mm dengan kategori sedang. Dapat dikatakan terdapat pengaruh aktivitas antibakteri pada Daun Pepaya (Carica papaya L) dengan bakteri Escherichia coli maka, semakin tinggi konsentrasi maka daya hambat yang dihasilkan semakin tinggi.
Aktivitas Antibakteri Daun Pepaya (Carica Papaya) Menggunakan Pelarut Etanol Terhadap Bakteri Bacillus subtilis Lestari Sudarwati, Tri Puji
Journal of Pharmacy and Science Vol. 3 No. 2 (2018): Journal of Pharmacy and Science
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v3i2.105

Abstract

ABSTRAKDaun pepaya banyak digunakan masyarakat sebagai obat tradisional. Daun pepaya mengandung senyawa antibakteri seperti tannin, alkaloid, flavonoid, terpenoid, saponin dan alkaloid karpain. Bakteri Bacillus subtilis merupakan  bakteri yang mengontaminasi makanan dan dapat menyebabkan infeksi gastroenteritis. Tujuan penelitian untuk mengetahui kemampuan ekstrak daun pepaya (Carica papaya L) dalam menghambat pertumbuhan bakteri Bacillus subtilis. Jenis penelitian ini adalah eksperimen laboratorium. Uji daya hambat menggunakan metode difusi kertas cakram. Variabel penelitian yaitu konsentrasi ekstrak daun pepaya 20 µg/mL, 40 µg/mL, 60 µg/mL, 80 µg/mL, 100 µg/mL dan zona hambat pertumbuhan bakteri Bacillus subtilis. Hasil penelitian ini didapatkan ekstrak daun papaya (Carica papaya L) dapat menghambat pertumbuhan bakteri Bacillus subtilis pada konsentrasi 20% sampai 100% dengan rata – rata diameter zona hambat 8,1 mm sampai dengan 8,6 mm dengan kategori sedang. Hal ini menunjukkan bahwa ekstrak daun pepaya (Carica papaya L) mempunyai pengaruh terhadap pertumbuhan bakteri Bacillus subtilis.Kata kunci: Daun Pepaya,Etanol, Bacillus subtilis.ABSTRACTPapaya leaves are common to use as a traditional medicine for society. Papaya leaves contain of anti-bacteria compound, such as tannins, alkaloids, flavonoids, terpenoids, saponins, and karpain alkaloids. Bacillus subtilis is a bacteria that contaminates food and can cause gastroenteritis infection. The purpose of this observation is to find out the ability of papaya leaves extract towards the obstruction of Bacillus subtilis bacteria. The observer conducts a laboratory experiment. To conduct obstruction power test, the observer uses disc paper diffusion method. The observation variable measures the papaya leaves extract concentration in 20 µg/mL, 40 µg/mL, 60 µg/mL, 80 µg/mL, 100 µg/mL and the obstruction zone growth of Bacillus subtilis bacteria. Hence, the result shows that papaya leaves (Carica papaya L) extract successfully obstruct the growth of Bacillus subtilis bacteria in a concentration range 20 % to 100 % with the diameter zone average 8,1 mm to 8,6 mm in a medium category. Thus, it shows that papaya leaves (Carica papaya L) extract significantly influence the growth of Bacillus subtilis bacteria.Key Words: Papaya leaves, Ethanol, Bacillus subtilis bacteria.
Potensi Antimikroba Ekstrak Ethanol Ganoderma lucidum Menggunakan Metode Bioautografi terhadap Bakteri Escherichia coli dan Bacillus subtillis Sudarwati, Tri Puji Lestari; Fernanda, M.A. Hanny Ferry
Journal of Pharmacy and Science Vol. 6 No. 1 (2021): Journal of Pharmacy and Science
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v6i1.212

Abstract

Potensi antibakteri ekstrak ethanol jamur lingzhi (Ganoderma lucidum) menggunakan metode analisis KLT bioautografi terhadap Escherichia coli dan Bacillus subtillis sebagai penelitian pendahuluan untuk mengetahui kandungan metabolit sekunder pada jamur lingzi. Soxhletasi merupakan metode untuk mengekstraksi serbuk jamur lingzhi menggunakan pelarut etanol. Bioautografi merupakan metode yang digunakan untuk mengetahui kandungan metabolit sekunder pada jamur lingzi dan potensi antibakteri. Konsentrasi yang digunakan yaitu: 2%, 4%, 6%, 8%dan 10%, dengan pengulangan sebanyak 5 kali. KLT bioautografi menggunakan n-butanol : Asam asetat glasial : Air (4:1:5) sebagai eluent. Analisis yang telah dilakukan menghasilkan 3 noda dengan Rf 0,5 dengan warna kuning, Rf 0,87 warna biru, Rf 0,93 warna coklat, dengan daerah hambatan pada noda coklat dengan Rf 0,93 merupakan senyawa triterpen. Hasil dari pengukuran nilai rata-rata zona hambat yang diperoleh terhadap bakteri E.coli adalah sebagai berikut, pada konsentrasi 2% 5,45 mm dengan kategori lemah, konsentrasi 4% 5,14 mm, konsentrasi 6% 5,48 mm, konsentrasi 8% 5,94 mm dan konsentrasi terbesar terdapat pada konsentrasi 10% 6,98 mm dengan kategori sedang. Pada bakteri Bakteri Bacillus subtillis konsentrasi 2% Sebesar 5,9 mm, Konsentrasi 4% Sebesar 6,34 mm, Konsentrasi 6% Sebesar 6,68 mm, Konsentrasi 8% Sebesar 7,88 mm, Dan Konsentrasi 10% Sebesar 8,6 mm dengan Kategori Sedang Maka dapat disimpulkan bahwa ekstrak jamur lingzhi memiliki aktivitas antibakteri terhadap bakteri Escherichia coli. Sehingga semakin besar konsentrasi yang digunakan, semakin besar pula aktivitasnya dalam menghambat pertumbuhan bakteri.
Analisis GCMS terhadap Senyawa Fitokimia Ekstrak Metanol Ganoderma lucidum Surahmaida Surahmaida; Tri Puji Lestari Sudarwati; Junairiah Junairiah
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.637 KB) | DOI: 10.20473/jkr.v3i2.12060

Abstract

AbstrakPenelitian ini bertujuan untuk menganalisis senyawa fitokimia ekstrak metanol Ganoderma lucidum dengan metode GCMS. Tahapan penelitian ini meliputi  proses ekstraksi serbuk halus G. lucidum menggunakan pelarut metanol dengan metode maserasi selama 3x3 hari dan ekstrak metanol G. lucidum yang didapatkan dianalisis dengan alat GCMS. Senyawa fitokimia yang teridentifikasi oleh GCMS antara lain thiophene, 2-hexyl-; 3-((3-Acetoxythyl)-6-acetoxymethyl-2,4-dimethyl)phenyl)-2-methyl-(E)-2-propenyl acetate; 2,7-Diphenylindole; dan 5-(2-Bromophenyl)-7-chloro-2,3-dihydro-1H-1,4-benzodiazepin-2-one. Dapat disimpulkan bahwa senyawa fitokimia pada ekstrak metanol G. lucidum memiliki efek farmakologis bagi kesehatan manusia dan aktivitas biologis lainnya.  Katakunci: Ganoderma lucidum, senyawa fitokimia, GCMS AbstractThis study aims to analyze the phytochemical compounds of methanol extract of Ganoderma lucidum using the GCMS method. The stages of this study include extraction of fine powder G. lucidum using methanol solvent by maceration method for 3x3 days and methanol extract of G. lucidum obtained were analyzed by GCMS. Phytochemical compounds identified by GCMS is thiophene, 2-hexyl-; 3 - ((3-Acetoxythyl) -6-acetoxymethyl-2,4-dimethyl) phenyl) -2-methyl- (E) -2-propenyl acetate; 2,7-Diphenylindole; and 5- (2-Bromophenyl) -7-chloro-2,3-dihydro-1H-1,4-benzodiazepine-2-one. It can be concluded that phytochemical compounds in methanol extract of G. lucidum have pharmacological effects on human health and other biological activities. Keywords: Ganoderma lucidum, phytochemical compounds, GCMS
PENINGKATAN PENGETAHUAN MASYARAKAT MENGENAI PERKEMBANGAN DAN PENYEBARAN DBD Tri Puji Lestari Sudarwati; M.A Hanny Ferry Fernanda; Mercyska Suryandari
Jurnal Abdi Masyarakat Kita Vol 1 No 1 (2021): Jurnal Abdi Masyarakat Kita
Publisher : APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1176.178 KB) | DOI: 10.33759/asta.v1i1.92

Abstract

This community service event was aimed to elevate the knowledge of local society regarding the current update of Aedes aegypti mosquito as a vector of Dengue Hemorrhagic Fever (DHF). Hence, it raised awareness of their role in breaking the disease transmission. Involving students as supporting team, this activity was carried out at Jemur Gayungan RW 01 by giving public counselling and mentoring for the larvae surveyor cadre (Jumantik). Abdimas, which has been done, adds to the knowledge of jumantik cadres about the proliferation and spread of dengue, so that jumantik cadres not only observe their environment but can also take action to break the chain of dengue spread.
POTENSI ANTIMIKROBA EKSTRAK DAUN PEPAYA (Carica papaya L.) MENGGUNAKAN METODE BIOAUTOGRAFI TERHADAP BAKTERI Streptococcus sp Tri Puji Lestari Sudarwati
Jurnal Komunitas Farmasi Nasional Vol. 1 No. 1 (2021): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Carica papaya L is one of herbal plant that has been known as a traditional medicine of bacterial infections with various bioactivities such as an antiseptic, antiinflammatory, antifungal and antibacterial. This research aims to determine the antibacterial activity of the extracts ethanol leaf of papaya (Carica papaya L) against Streptococcus sp. Extract ethanol were obtained by maceration method and were tested using bioautography method. The extracts ethanol leaf of papaya can inhibit growth of Streptococcus sp at consentrations 2%, 4%, 6%, 8% and 10% with average inhibitory zone diameter that are 5,52 mm; 6,34 mm; 7,06 mm; 7,72 mm and 8,9 mm. TLC bioautography assay with eluen n-butanol : acetate acid : aquadest (4 : 1 : 5) is obtained inhibitory zone at stain with Rf value 0,93. Result of identification shown that the stain are tannin. Statistical result of Anova Oneway showed significant difference from each concentration.
UJI ANTIMIKROBA FRAKSI III DAUN KRATOM (Mitragyna speciosa) TERHADAP BAKTERI Staphylococcus aureus Tri Puji Lestari Sudarwati
Jurnal Komunitas Farmasi Nasional Vol. 1 No. 2 (2021): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kratom (Mitragyna speciosa Korth) is a type of plant found in forest areas in Indonesia which contains chemical compounds such as alkaloids, flavonoids, phenols, terpenoids, steroids, triterpenoids, tannins, and saponins. In this study, the antimicrobial potency of fraction III of kratom leaves was tested by column chromatography separation. Kratom leaves have several benefits, one of which is as an antimicrobial. The extraction process chosen is the maceration method. The results obtained from column chromatography were then grouped based on RF values ​​using the thin layer chromatography method, from 80 vials the RF value calculated in the TLC test of kratom leaf methanol extract obtained a value of 0.84. Then the results obtained were made with concentrations of 2%, 4%, 6%, 8% and 10% for the antibacterial activity test. The antibacterial activity test was repeated 5 times and the results of the inhibition zone with an average concentration of 2% obtained data of 2,436 mm, a concentration of 4% obtained an average of 1,616 mm, a concentration of 6% obtained an average of 0.685 mm, a concentration of 8% obtained an average of 0.685 mm. an average of 0.596 mm, while for a concentration of 10% the average was 0.96. From the results of this study, it can be concluded that kratom leaf extract (Mitragyna speciosa) using methanol with column chromatography separation method has antibacterial activity against Staphylococcus aureus with a weak category.
Fraksinasi dan Identifikasi Ektrak Daun Mitragyna Speciosa Menggunakan Metode Kromatografi Fernanda, M.A. Hanny Ferry; Suryandari, Mercyska; Sudarwati, Tri Puji Lestari
FARMASIS: Jurnal Sains Farmasi Vol 2 No 2 (2021): Farmasis : Jurnal Sains Farmasi
Publisher : Universitas PGRI Adi Buana, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/farmasis.v2i2.4072

Abstract

Background: Mitragyna Speciosa or better known as Kratom is a typical plant from the Putusibau area, West Kalimantan. The most widely used part of this plant is the leaves. The kratom plant contains alkaloids, flavonoids, phenols, terpenoids-steroids, triterpenoids, saponins, and tannins. Alkaloid content is the most dominant and has benefits in this plant so it is necessary to do fractionation and identification of secondary metabolites. Objective: To fractionate and identify secondary metabolites from Mitragyna Speciosa leaf extract. Methods: The fractionation method was carried out using Column Chromatography while for identification using Thin Layer Chromatography. Results: 80 fractions were obtained from the column chromatography results, while the results of identification using thin layer chromatography showed that only the 2nd and 3rd fractions were obtained. The second fraction has two spots with Rf values of 0.51 and 0.73 and in the third fraction there are three spots with Rf values of 0.21; 0.52, and 0.74. Conclusion: Fractionation and identification by chromatographic method can be used as initial screening for secondary metabolite content in Mitragyna Speciosa leaf extract.
Co-Authors Fernanda, M.A. Hanny Ferry Junairiah Junairiah M. A Hanny Ferry Fernanda M. A Hanny Ferry Fernanda M.A Hanny Ferry Fernanda Mercyska Suryandari Surahmaida, Surahmaida