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All Journal AL-HAYAT: Journal of Biology and Applied Biology Jurnal Kesehatan Tambusai
Abdelrazig, Omnia Amir Osman
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media Abdelrazig, Omnia Amir Osman; Fadilah, Fadilah; Prasetyo, Dimas Seto; Saharman, Yulia Rosa; Hegar, Badriul
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20528

Abstract

The prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population.
Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media Abdelrazig, Omnia Amir Osman; Fadilah, Fadilah; Prasetyo, Dimas Seto; Saharman, Yulia Rosa; Hegar, Badriul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20528

Abstract

The prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population.
Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media Abdelrazig, Omnia Amir Osman; Fadilah, Fadilah; Prasetyo, Dimas Seto; Saharman, Yulia Rosa; Hegar, Badriul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20528

Abstract

The prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population.
A DAMPAK ASURANSI KESEHATAN TERHADAP AKSES PELAYANAN KESEHATAN IBU : TINJAUAN LITERATUR DI KONTEKS GLOBAL DAN INDONESIA Wulandari, Reny Yulita; Rachmawan, Ade; Alridjal, Rifqi; Abdelrazig, Omnia Amir Osman
Jurnal Kesehatan Tambusai Vol. 6 No. 3 (2025): SEPTEMBER 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v6i3.48470

Abstract

Akses terhadap pelayanan kesehatan ibu yang aman, berkualitas, dan terjangkau masih menjadi tantangan utama di banyak negara berpendapatan rendah dan menengah, termasuk Indonesia. Salah satu strategi yang digunakan untuk mengatasi hambatan tersebut adalah penerapan asuransi kesehatan, baik dalam bentuk skema nasional maupun berbasis sosial. Studi ini merupakan tinjauan pustaka sistematis yang bertujuan untuk menganalisis dampak asuransi kesehatan terhadap pemanfaatan layanan kesehatan ibu, dengan mengkaji sembilan artikel ilmiah dari konteks global dan nasional. Hasil tinjauan menunjukkan bahwa asuransi kesehatan secara umum meningkatkan angka kunjungan antenatal care (ANC) dan persalinan di fasilitas kesehatan, serta memberikan perlindungan finansial bagi kelompok rentan. Namun, manfaat tersebut belum dirasakan secara merata, mengingat masih adanya hambatan geografis, sosial, dan budaya yang membatasi akses layanan, khususnya bagi ibu di daerah terpencil. Selain itu, persepsi terhadap mutu layanan terbukti menjadi faktor penentu dalam keputusan ibu untuk mengakses layanan, yang dalam beberapa kasus bahkan lebih dominan dibandingkan kepemilikan asuransi. Oleh karena itu, keberhasilan program asuransi kesehatan sangat bergantung pada peningkatan mutu layanan, pemerataan sumber daya kesehatan, serta penguatan literasi masyarakat. Kajian ini merekomendasikan pendekatan kebijakan yang lebih holistik dan responsif untuk memastikan manfaat asuransi dapat berkontribusi signifikan dalam menurunkan angka kematian ibu dan meningkatkan keadilan dalam pelayanan kesehatan.
Co-Authors Alridjal, Rifqi Badriul Hegar Dimas Seto Prasetyo Fadilah Fadilah, Fadilah Rachmawan, Ade Wulandari, Reny Yulita Yulia Rosa Saharman