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Potensi Kombinasi Ekstrak Bawang Putih dan Polifenol sebagai Metode Kuratif Pengendalian Ganoderma boninense Achmad Yusup, Ciptadi; Dewantara Eris, Deden; Sri Mulyatni, Agustin; Gama Pradana, Mahardika; Widiastuti, Happy; Santoso, Djoko
Jurnal Penelitian Kelapa Sawit Vol 32 No 2 (2024): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v32i2.259

Abstract

Pengendalian penyakit busuk pangkal batang (BPB) hingga saat ini berfokus kepada kegiatan preventif, sehingga diperlukan teknik pengendalian secara kuratif untuk melengkapi teknik pengendalian yang sudah ada. Penggunaan fungisida organik merupakan salah satu solusi yang ramah lingkungan. Ekstrak bawang putih (GE) dan polifenol (P) memiliki aktivitas antifungal terhadap berbagai jenis cendawan. Kombinasi kedua senyawa ini diharapkan mampu membentuk interaksi sinergistik yang efektif mengendalikan G. boninense secara kuratif. Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas antifungal kombinasi GE dan P (GE+P) terhadap G. boninense, menentukan konsentrasi optimum yang dapat mematikan G. boninense secara in vitro, serta membuat formulasi fungisida organik dari GE+P. Pengujian in vitro menggunakan teknik peracunan media, sedangkan untuk uji kompatibilitas menggunakan metode checkerboard assay. Evaluasi kapasitas pertumbuhan inokulum G. boninense yang diuji dilakukan dengan Bavendamm test. Sedangkan untuk evaluasi efek kuratif kombinasi GE+P dilakukan dengan uji tetes dan semprot pada koloni G. boninense. Pengamatan kerusakan miselium akibat pengujian kontak dilakukan dengan scanning electron microscope (SEM). Hasil penelitian menunjukkan bahwa kombinasi GE+P memiliki interaksi sinergistik dan mampu merusak jaringan miselium G. boninense pada konsentrasi minimum 0,4%. Pengamatan SEM menunjukkan kerusakan parah jaringan miselium G. boninense pada aplikasi dengan konsentrasi 1,6%. Formulasi fungisida organik kombinasi GE+P berhasil membentuk larutan yang homogen dan stabil dengan penambahan surfaktan sodium lauryl sulfate (SLS). Pengujian daya simpan formulasi fungisida organik menunjukkan kestabilan dan tidak adanya penurunan efektivitas setelah disimpan selama enam bulan.
The Effect of Storage Period and Mixing Techniques on the Chemical Content of Environmentally Friendly BIOPOS Organic Compost at SEAMEO BIOTROP Rosita, Risa; Imran, Zulhamsyah; Fitra, Allegra Aura; Dewi, Amelia Cantrika; Bayuaji, David Yudhoyono; Dewantara Eris, Deden; Widayanti, Sri; Ayu Astari, Risya; Purnajaya, Agus
BIODIVERS - BIOTROP Science Magazine Vol. 3 No. 1 (2024): BIODIVERS (BIOTROP Science Magazine)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56060/bdv.2024.3.1.2227

Abstract

SEAMEO BIOTROP produces daily waste from oyster mushroom baglog (Pleurotus ostreatus), dry organic waste that contains little water, such as dry leaves, grass straw, and citronella distilled waste. Managing oyster mushroom baglog waste, leaf waste, grass straw, and citronella distilled waste independently at SEAMEO BIOTROP as organic compost BIOPOS (BIOTROP Compost) is an environmentally friendly practice and can provide benefits both economically and ecologically. Apart from being carried out to protect biodiversity from decline, this study can also keep the soil system healthy and sustainable. The aim of this research is to: (1) make BIOPOS organic compost through the use of oyster mushroom baglog, leaf waste, leaf straw, and citronella distilled waste; (2) determine the influence of various types of BIOPOS organic compost (KT1, KT2, KT3) on the chemical content of compost. Research stages include: (1) creating BIOPOS organic compost, (2) measuring the chemical content of BIOPOS organic compost, and (3) measuring the effectiveness of BIOPOS. During the storage period of 30 dam up to 180 dam, BIOPOS organic compost which still contains N (0,54%), P (36,8%), K (7,23%) and complies with SNI 19-7030-2004 quality standards is KT3. In measuring C-Organic value, BIOPOS code KT2 with a storage period of 30 dam gained C-Organic amount of 28,36%, satisfying quality standards. The application of BIOPOS KT1, KT2, and KT3 with a pH range of 8,2- 8,3 high pH can be used to correct problems that often occur in acidic soils and is not effective when applied to sub-optimal alkaline soils because it will cause soil pH to increase sharply. Key words: BIOPOS, citronella distilled waste, grass straw, leaf waste, oyster mushroom baglog waste
Spore Production and Inoculum Formulation of Claroideoglomus Etunicatum and its Application in Maize (Zea Mays) Rosita, Risa; Dewantara Eris, Deden; Aslan, Aslan; Imran, Zulhamsyah
BIODIVERS - BIOTROP Science Magazine Vol. 3 No. 2 (2024): BIODIVERS Volume 3 No. 2 (2024)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56060/bdv.2024.3.2.2297

Abstract

Recently, the demand for AMF (Arbuscular Mycorrhiza Fungi) fertilizer for agriculture, plantation, and forestry in Indonesia has been very high. Meanwhile, the unavailability of an applicable AMF inoculum that's easy to use and inexpensive is one of the constraints on AMF application as a fertilizer in the field. It indicates that the best formulated inoculum must be found. The study aimed to produce AMF spores using conventional and hydroponic system, formulate the best AMF inoculum, and measure the growth response in maize (Zea mays) after applying the formulated inoculum. In the formulation stage, the study was designed using a Completely Randomized Design (CRD) with 1 factor. The factor was the type of formulation. Data were analyzed using SAS software version 9 and further tested using Duncan's Multiple Range Test (DMRT) at 5% alpha (α) level. The results reported that the conventional pot culture technique (CVNE2) treatment that had been inoculated by Claroideoglomus etunicatum produced the highest spore density amount of 350 ± 6.97 per 10 g of zeolites (P<0.05). On the other hand, the hydroponic NFT (NFTE2) treatment that had been inoculated by C. etunicatum obtained the highest AMF percent colonization amount of 98% ± 2.66% (P<0.05). Analysis of variance in the formulation stage showed TZ60F1 treatment had a significant effect (P<0.05) in increasing plant height, the number of leaves, and dry weight of Zea mays, respectively 120 cm ± 1.7 cm; 15 ± 0.84; 26.3 g ± 2.46 g in 3 months after application.