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All Journal HAYATI Journal of Biosciences
Tafroji, Wisnu
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Gut Microbiota Profile of Infants with Breastfeeding and Mixed Feeding Patterns Kusumaningrum, Tina; Tafroji, Wisnu; Gultom, Septiani Madonna; Putri, Nina Dwi; Hafifah, Cut Nurul; Safari, Dodi
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.530-538

Abstract

We explore the gut microbiota profiles of 103 stool samples collected from infants at the age of 4 and 6 months in Jakarta, Indonesia. We performed 16S rRNA gene sequencing with Illumina MiSeq to identify the diversity, structure, and composition of the gut microbiota from those stool samples. Among 103 stool samples, 55 and 48 samples were collected from infants with breastfeeding and mixed feeding patterns, respectively. We found that the most abundant bacteria were Bifidobacteriales from the phylum of Actinobacteria (43.05%), Lactobacillales from the phylum of Firmicutes (28.39%), and Enterobacterales from the phylum of Proteobacteria (13.75%). The alpha and beta diversity analysis showed that the association between feeding patterns and differences in the microbial communities was not statistically significant (p-value >0.05). Our study did not show a difference in the gut microbiota pattern between the two feeding pattern groups. This result contributed to the variety of the world gut microbiota profile data in infants.
Immune Response to Capsular Polysaccharide of Streptococcus pneumoniae in Rabbits Immunized with Pneumococcal Conjugate Vaccine Zaenab; Tafroji, Wisnu; Salsabila, Korrie; Winarti, Yayah; Wulandari, I Gusti Ayu Inten; Yasmon, Andi; Safari, Dodi
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.642-648

Abstract

To evaluate the immune response of a rabbit model to polysaccharide capsules of Streptococcus pneumoniae after primary and booster immunization with pneumococcal conjugate vaccine. Rabbits were immunized with 0.25 ml of the 13-valent pneumococcal conjugate vaccine (PCV13) on Day 0, 7, and 14. Blood of rabbits was collected to measure the specific antibodies against the native polysaccharide capsules of S. pneumoniae serotypes 6B (Anti-Pn6BPS antibodies) and 19F (Anti-19FPS antibodies) using enzyme-linked immunosorbent assay (ELISA). The specific anti-Pn6BPS and anti-Pn19FPS antibodies in the immunized group increased significantly compared to the control rabbit after immunization (P<0.05). The highest titer of anti-Pn6BPS and anti-Pn19FPS antibodies were obtained on Day 21 and Day 28, respectively. The third immunization on Day 21 is a fairly high increase in specific antibodies against polysaccharide type 6B and type 19F. The highest antibody titers against polysaccharide capsules of S. pneumoniae were obtained after booster immunization.
Co-Authors Andi Yasmon Gultom, Septiani Madonna Hafifah, Cut Nurul Kusumaningrum, Tina Nina Dwi Putri Safari, Dodi Salsabila, Korrie Winarti, Yayah Wulandari, I Gusti Ayu Inten Zaenab