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All Journal Jurnal Biologi Tropis Bioscientist : Jurnal Ilmiah Biologi
Andayani, I Gusti Ayu Sri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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In-Silico Study to Design Foot and Mouth Disease Vaccine Candidates Andayani, I Gusti Ayu Sri; Depamede, Sulaiman Ngongu; Dradjat, Adji Santoso; Sriasih, Made
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7493

Abstract

Foot and Mouth Disease (FMD) is a viral disease that affects livestock and can cause significant economic losses.  Vaccination has been recognized as the primary strategy for FMD prevention, but vaccine development, especially conventional vaccine production, has time, cost, and effectiveness limitations. Using software-based immunoinformatics methods has cost and time efficiency for simulation development and calculations in vaccine development research.  This study aimed to design FMD vaccine candidates using an epitope-based in-silico approach, focusing on identifying potential epitopes of the pathogen that causes FMD. The in silico approach was used to analyze FMD virus genome sequences from UniProtKB (https://www.ebi.ac.uk/Tools/msa/clustalo) and the National Library of Medicine application (https://www.ncbi.nlm.nih.gov).  Furthermore, the prediction of FMD virus epitopes was analyzed using the Immuno-Epitope database, and the prediction of peptide antigenicity levels using VaxiJen 2.0 software. The analysis identified eight potential epitope candidates, such as CSIQKR, TEFGFHPNA, EIRPMEKVRA, YEGVEL, SFARRGT, APGLPWALQGKRRGALIDFESGTV, MASLEDKGKPF, and TLPTSFNYGAI with antigen binding affinities of 2. 9424nM, 1.65nM, 1.4702nM, 1.2966nM, 1.2643nM, 1.1967nM, 1.1921nM and 1.0143nm respectively. These epitopes are expected to form the basis for developing more selective and safe peptide vaccines. By focusing on epitopes, the resulting vaccine can improve the effectiveness and safety of vaccination and accelerate the development of new vaccines for FMD prevention.
Performa Rapid Diagnostik Tes Hepatitis B dengan ELISA sebagai Gold Standar Andayani, I Gusti Ayu Sri; Rizki, Mohammad; Sriasih, Made; Tauhida, Ika Nurfitria
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9035

Abstract

The ELISA (Enzyme Linked Immunoassay) method has high sensitivity and specificity, qualitatively and quantitatively detecting Hepatitis B Surface Antigen (HBsAg) levels, but the process is long, expensive and requires special expertise, compared to the Rapid Diagnostic Test (RDT) as a rapid test which is more practical and cheaper. The aim of this study was to compare the sensitivity and specificity of the Entebe Rapid Test Diagnostic (ERTD) produced by PT. Hepatika Mataram, with ELISA as the gold standard in detecting HBsAg. The requirement for interpreting sensitivity and specificity results according to World Health Organization (WHO) standards is a minimum of 95%. Testing with a negative panel of 00 samples and a positive panel of 1 samples from Papua in 012 at -80℃ storage. Testing in April 023 at the Molecular Diagnostics and Research Laboratory, Mataram University Hospital. Statistical analysis using MedCal software. ERD HBsAg produced by PT. Hepatika Mataram has a sensitivity of 95.45%, specificity of 99.50%, positive predictive value of 93.59%, negative predictive value of 99.65%, and accuracy of 99.21%.
Perbandingan Metode Isolasi Phosvitin dari Kuning Telur Ayam Buras untuk Karakterisasi Aktivitas Bioaktif Andayani, I Gusti Ayu Sri; Iswaini, Dedy; Depamede, Sulaiman N; Kisworo, Djoko; Bulkaini, Bulkaini; Sriasih, Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14304

Abstract

This study aims to compare phosvitin isolation procedures from chicken egg yolk based on purity level, time efficiency, and functional activity. The procedures compared include previously published methods and modified methods developed in this study. Phosvitin was isolated from 10 samples of native chicken eggs collected from farms in the Mataram area using a stratified random sampling approach. The isolation process involved several sequential steps: sedimentation, centrifugation, dialysis, and lyophilization. A comparative analysis of the two procedures was conducted based on protein profile characteristics using SDS-PAGE and Fourier Transform Infrared (FTIR) spectroscopy to identify phosphate group interactions and metal ion binding. SDS-PAGE results showed that both procedures yielded α-phosvitin (35 kDa) and β-phosvitin (45 kDa) protein bands. FTIR spectra confirmed that both phosvitin samples contained high levels of phosphate groups, indicating strong phosphorylation and good protein stability. From a chemical and biological standpoint, the modified procedure showed no qualitative differences compared to the previously established method. However, the modified protocol was approximately 30 minutes faster and did not require a pH adjustment step, making it a simpler and more time-efficient alternative for isolating phosvitin from egg yolk.
Co-Authors Adji Santoso Dradjat Bulkaini (Bulkaini) Depamede, Sulaiman N Djoko Kisworo Iswaini, Dedy Made Sriasih, Made Rizki, Mohammad Sulaiman Ngongu Depamede Tauhida, Ika Nurfitria