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All Journal Jurnal Biologi Tropis Bioscientist : Jurnal Ilmiah Biologi
Depamede, Sulaiman N
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Prevalence of helminthiasis and identification of worm species in buffaloes on smallholder farms in Jerowaru sub-district, East Lombok Sriasih, Made; Depamede, Sulaiman N; Wariata, Wayan; Ali, Muhamad; Rosyidi, Anwar; Hasma, Hasma; Hadi, Imron
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6506

Abstract

Jerowaru sub-district is one of 21 sub-districts in East Lombok district. Many people raise buffaloes to fulfill their need for food of animal origin. However, the business is still hampered by diseases caused by worm infestation, which is economically detrimental. This study aimed to obtain data on the prevalence of helminthiasis in buffaloes and identify the types of worms that infest the animals. Fecal samples were collected from buffaloes kept in smallholder farms in several villages in the Jerowaru sub-district. Examination and identification of samples were conducted at the Microbiology and Biotechnology laboratory of the Faculty of Animal Science, University of Mataram, and the Banyumulek Animal Health laboratory. The results show that the prevalence of helminthiasis in buffaloes kept in smallholder farms in the Jerowaru subdistrict was 14.60%. The types of parasites infecting buffaloes consisted of Eimeria (13.48%) and Toxocara (1.12%). Although parasite infection intensity is categorized as mild, it is necessary to pay attention to the cleanliness of cattle pens, especially during the rainy season, so they are not wet and humid.
Perbandingan Metode Isolasi Phosvitin dari Kuning Telur Ayam Buras untuk Karakterisasi Aktivitas Bioaktif Andayani, I Gusti Ayu Sri; Iswaini, Dedy; Depamede, Sulaiman N; Kisworo, Djoko; Bulkaini, Bulkaini; Sriasih, Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14304

Abstract

This study aims to compare phosvitin isolation procedures from chicken egg yolk based on purity level, time efficiency, and functional activity. The procedures compared include previously published methods and modified methods developed in this study. Phosvitin was isolated from 10 samples of native chicken eggs collected from farms in the Mataram area using a stratified random sampling approach. The isolation process involved several sequential steps: sedimentation, centrifugation, dialysis, and lyophilization. A comparative analysis of the two procedures was conducted based on protein profile characteristics using SDS-PAGE and Fourier Transform Infrared (FTIR) spectroscopy to identify phosphate group interactions and metal ion binding. SDS-PAGE results showed that both procedures yielded α-phosvitin (35 kDa) and β-phosvitin (45 kDa) protein bands. FTIR spectra confirmed that both phosvitin samples contained high levels of phosphate groups, indicating strong phosphorylation and good protein stability. From a chemical and biological standpoint, the modified procedure showed no qualitative differences compared to the previously established method. However, the modified protocol was approximately 30 minutes faster and did not require a pH adjustment step, making it a simpler and more time-efficient alternative for isolating phosvitin from egg yolk.
Co-Authors Andayani, I Gusti Ayu Sri Anwar Rosyidi Bulkaini (Bulkaini) Djoko Kisworo Hasma Hasma Imron Hadi Iswaini, Dedy Made Sriasih, Made Muhamad Ali Wariata, Wayan