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All Journal Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Acta Holistica Pharmaciana Borneo Journal of Pharmacy
Ramadhan, Muhammad Marwan
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Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Pengaruh Metode Ekstraksi terhadap Aktivitas Antioksidan Ekstrak Etanol Daun Singkil (Premna corymbosa Rottl. et Willd) dengan DPPH secara Spektrofotometri UV-Vis Jubaidah, Siti; Wijaya, Heri; Safira, Alya; Ramadhan, Muhammad Marwan
Acta Holistica Pharmaciana Vol 6 No 1 (2024): Acta Holistica Pharmaciana
Publisher : School of Pharmacy Mahaganesha (Sekolah Tinggi Farmasi Mahaganesha)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62857/ahp.v6i1.160

Abstract

Daun singkil (Premna corymbosa Rottl. et Willd) merupakan salah satu tumbuhan yang mengandung senyawa flavonoid, yang memiliki aktivitas antioksidan. Metode ekstraksi yang tepat akan menentukan banyaknya jumlah bahan aktif yang didapat dalam suatu ekstrak. Tujuan penelitian ini adalah mengetahui aktivitas daun singkil berdasarkan perbedaan metode ekstraksi yaitu maserasi, refluks dan soxhlet.Penelitian yang dilakukan bersifat eksperimental dengan tahapan dimulai dari pengumpulan bahan baku, determinasi tumbuhan, pembuatan simplisia, pembuatan ekstrak dengan metode maserasi, refluks dan soxhlet menggunakan pelarut etanol 70%, dan pengujian aktivitas antioksidan dengan DPPH (1,1-Difenil-2-Pikrilhidrazil) secara spektrofotometri UV-Vis. Analisis data yang dilakukan menggunakan statistik Kruskal-Wallis.Berdasarkan hasil penelitian dengan uji Kruskal-Wallis nilai signifikan (0,180) > nilai p-value 0,05 maka perbedaan metode ekstraksi tidak berpengaruh terhadap aktivitas antioksidan ekstrak daun singkil. Hasil aktivitas antioksidan ekstrak daun singkil pada metode maserasi 40,56 ppm, refluks 41,59 ppm dan soxhlet 35,66 ppm. Aktivitas antioksidan dari ekstrak daun singkil termasuk sangat kuat.
Purple Yam (Dioscorea alata) Extract Increasing Dopamine Levels and Improving the Brain's Microscopic Features in Parkinson's Model Mice Yuliani, Sapto; Utami, Dwi; Nurani, Laela Hayu; Ramadhan, Muhammad Marwan; Ainiyah, Nadia Putri; Bachri, Mochammad Saiful; Widyaningsih, Wahyu; Amukti, Danang Prasetyaning
Borneo Journal of Pharmacy Vol. 8 No. 2 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i2.7267

Abstract

Parkinson's disease (PD) is a severe neurodegenerative disorder, that causes progressive motor issues from the loss of dopamine-producing neurons in the substantia nigra pars compacta (SNpc). Purple yam (Dioscorea alata), rich in anthocyanins, shows promise as a natural antioxidant and neuroprotectant. This study investigated the antiparkinsonian effects of D. alata extract on dopamine levels and brain microscopic features in a haloperidol-induced PD mouse model. Thirty-five male mice were randomly allocated into seven groups: normal (CMC-Na and aqua pro injection), haloperidol-induced negative control (CMC-Na), positive control (levodopa 39 mg/kgBW), curcumin (200 mg/kgBW), and D. alata extract-treated groups (100, 200, and 400 mg/kgBW). Treatments were administered daily for seven days. On day 8, all groups, except the normal control, received an intraperitoneal injection of haloperidol (2 mg/kgBW) to induce Parkinsonism. Three hours post-haloperidol injection, dopamine levels were measured from orbital vein blood. Subsequently, brains were harvested for histological examination of the SNpc using Toluidine blue staining. Data were statistically analyzed using one-way ANOVA followed by LSD post-hoc tests. The 400 mg/kgBW dose of D. alata extracts significantly increased dopamine levels (p<0.05) compared to the negative control group. Microscopic analysis of the SNpc in mice treated with 400 mg/kgBW extract revealed preserved, dark, and solid neuronal morphology, with significantly higher scoring results (p<0.05) when compared to the levodopa-treated group. These findings suggest that D. alata extract, particularly at a dose of 400 mg/kgBW, exhibits potential antiparkinsonian activity by elevating dopamine levels and mitigating dopaminergic neuronal damage in a haloperidol-induced PD mouse model.
In Silico Study of the Potency of Purple Yam Anthocyanin Compounds (Dioscorea alata L.) As MAO-B and COMT Inhibitors in Parkinson's Disease Ramadhan, Muhammad Marwan; Utami, Dwi; Yuliani, Sapto
Media Farmasi: Jurnal Ilmu Farmasi Vol. 21 No. 1: March 2024
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v21i1.26076

Abstract

The purple yam plant (Dioscorea alata L.) is an economically important staple food for millions of people in tropical and subtropical regions. Dioscorea spp. anthocyanin chemicals have been demonstrated to have antioxidant and neuroprotective properties. The purpose of this study was to explore the potency of anthocyanin compounds in purple yam as antiparkinsonian agents via the monoamine oxidase B (MAO-B) receptor (pdb: 2V5Z) and the catechol-O-methyltransferase (COMT) receptor (pdb: 6I3C) using a molecular docking technique. The study was divided into four stages: (1) pharmacokinetic and Lipinski Rule evaluation, (2) protein (receptor) and ligand preparation, (3) docking method validation, and (4) molecular docking for MAO-B and COMT proteins. Pharmacokinetic prediction and Lipinski rule evaluation revealed that cyanidin, delphinidin, and delphinidin-3-glucoside had an ADMED profile and met Lipinski’s rule. The docking results showed that the binding energy (G) of the compounds cyanidin, delphinidin, and delphinidin-3-glucoside to the MAO-B receptor was lower (-9.50 kcal/mol) than that of the natural ligands ((-4.79 kcal/mol). The Cys172, Leu 171, Ile198, Phe168, Pro104, Trp119 and the 'gatekeeper' residue Ile199 are the amino acids that are majoring involved in MAO-B inhibitors. At the COMT receptor, all the tested compounds had a higher binding energy than native ligands (> -4.79 kcal/mol) except for Cyanidin 3,5-diglucoside (-4.64 kcal/mol). The amino acids Trp143 and Pro174, ensure correct substrate orientation, Mg2+ ions, and cofactor SAM, as well as residues Lys144 and Glu199. In conclusion, this study showed that based on the molecular docking approach, the active compounds of purple yam namely cyanidin, delphinidin, and delphinidin-3-glucoside have the potential to be developed as anti-parkinsonian agents through MAO-B and COMT.
Co-Authors Ainiyah, Nadia Putri Bachri, Mochammad Saiful Danang Prasetyaning Amukti Dwi Utami Heri Wijaya, Heri Laela Hayu Nurani Muhammad Yusron Maulana El-Yunusi Safira, Alya Sapto Yuliani Siti Jubaidah, Siti Wahyu Widyaningsih