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All Journal Jurnal Biologi Tropis Jurnal Pembelajaran dan Biologi Nukleus
An Nisaa, Ranti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Exploration of High Economic Value Microalgaes in The Mangrove Area of Pari Island, Seribu Islands, Jakarta Hutari, Andri; An Nisaa, Ranti; Suhendra, Suhendra; Agustin, Yeni; Ayunda, Khairunisa Aprilia
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i3.3096

Abstract

Mangrove areas are widely reported as a place of abundance of microalgae producing omega-3 docosahexaenoic acid (DHA) and other bioactive materials of high economic value from the genus Aurantiochytrium. The search for microalgae was carried out in the mangrove area of Pari Island, Seribu Islands, Jakarta, in April 2022 and identified at the Biology Education Laboratory, FKIP UHAMKA. The purpose of this study is part of the long-term goal of collecting new strains or isolates from the genus Aurantiochytrium and other members of the family Thraustochytriidae from forests in Indonesian mangroves. This research was conducted by isolating microalgae with the pure streaking method and identifying them morphologically. This study succeeded in obtaining eight isolates (UHA1, UHA3, UHA5, UHA6, UHA10, UHA11, UHA12, and UHA13) which in the appearance of colonies and microscopic morphology lead to the species Aurantiochytrium sp. One of the isolates obtained (UHA12) was cultivated by batch culture for five days in an Erlenmeyer flask using glucose, yeasts extract, and seawater as a medium. The result is that the dry biomass obtained is almost equivalent to that of the Aurantiochytrium sp. other famous
Identification of Thraustochytrids from Mangrove Forest of Tidung Island, Kepulauan Seribu, Jakarta An Nisaa, Ranti; Hutari, Andri; Awaliah, Walidah; Permatasari, Renna; Ar Rasyid, Muhammad Luqman
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10133

Abstract

Thrautochytrids are often called heterotrophic microalgae that have a lot of omega-3 docosahexaenoic acid, or DHA. These microbes are found in many mangrove forest areas, including Indonesia, which has the biggest mangrove forests in the world. Indonesia is also beginning to look for useful microbes in these environments. The goal of this study was to find and identify a type of Thrautochytrid called Aurantiochytrium sp. in the mangrove forests of Tidung Island, part of the Thousand Islands near Jakarta. This area has never been studied for this type of microbe before. The study found two isolates of Thrautochytrids, named Aurantiochytrium sp. PTD5 and PTD6. These were identified by looking at the phylogenetic tree made from 18S rRNA sequences. The phylogenetic tree shows that PTD5 and PTD6 are closely related to other Aurantiochytrium strains like SR21 and BL10, which are widely used in research for making omega-3 DHA lipids.
Co-Authors Ar Rasyid, Muhammad Luqman Awaliah, Walidah Ayunda, Khairunisa Aprilia Hutari, Andri Permatasari, Renna Suhendra Suhendra Yeni Agustin