Ibrahim, Zurairah
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Cervical vertebral maturation stage and Demirjian index for assessment of skeletal and dental maturation for children’s growth stages Pradopo, Seno; Nuraini, Prawati; Rahmawati, Luluk; Ibrahim, Zurairah
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 1 (2025): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i1.p60-65

Abstract

Background: Determining age is essential in various fields, particularly pediatric dentistry. A reliable method for assessing an individual's growth and development involves evaluating their chronological and biological age. Biological age can be determined by examining skeletal or dental maturation. Accurately assessing growth potential and timing of growth spurts is crucial for several clinical situations, especially in the planning and outcomes of treatments like orthodontic therapy. Purpose: This study uses common radiographic ortho-diagnosis techniques to analyze the accuracy of cervical vertebral maturation stages (CVMS) and the Demirjian index methods in evaluating children's growth and development stages. Methods: The CVMS assessment on cephalometric radiography was conducted using the Bacetti method, which includes six stages. In addition, the mandibular second molars' calcification stages were evaluated using the Demirjian index method, which encompasses stages A to H on panoramic radiography. Following this, skeletal and dental maturation accuracy was analyzed using the Statistical Package for Social Sciences application. Results: This study revealed differences in the chronological age corresponding to each stage of calcification of the mandibular second molars and the CVMS. Additionally, CVMS was found to be the most accurate method for assessing age in children. Furthermore, the right side was generally preferred over the left at the calcification stages of the mandibular second molars. Conclusion: Using CVMS to assess skeletal maturation provides a more accurate determination of growth and developmental stages in children than the Demirjian index.
Saltwater Fish Powder for Amelogenesis in Zebrafish Larvae (Danio rerio) Christiono, Sandy; Pradopo, Seno; Hutami, Islamy Rahma; Indasari, Novia; Nurhapsari, Arlina; Rochmah, Yayun Siti Rochmah; Ibrahim, Zurairah; Yuniar, Savira Nurazky; Suparmi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.54677

Abstract

Graphical Abstract Highlight Research 1. The saltwater fish powder is rich in omega3, vitamin D, and calcium which helps tooth amelogenesis. 2. LC50 Toxicity Test was carried out using zebrafish larvae (Danio rerio) with negative control, positive control using 3,4-dichloroanilin, and treatment groups with saltwater fish powder solution in 6 different concentration. 3. No signs of zebrafish embryo mortality or developmental abnormalities on treatment group from the lowest (125μg/ml) nor highest concentration (4000μg/ml). 4. Saltwater fish powder has proven lack of toxicity. Abstract Many nutrients found in saltwater fish are thought to promote amelogenesis in ameloblast cells during tooth development. Although its toxicity is uncertain, saltwater fish powder provided benefits for amelogenesis. Zebrafish embryos have complex metabolic pathways and comprehensive physiological reactions. The LC50 toxicity of saltwater fish powder on zebrafish embryos was investigated in this study. The aim of the of the study is to determine the effect of LC50 toxicity on saltwater fish powder on zebrafish embryos. The experimental research method was a post-test-only control group design, consisting of eight groups, namely the negative control and the internal group using embryo media, the positive control using 3,4-dichloroanillin, and the treatment group 125μg / ml, 250μg / ml, 500μg / ml, 1000μg / ml, 2000μg / ml, and 4000μg / ml using 384 zebrafish embryos. Saltwater fish powder is diluted and observed for 96 hours, controlled every 24 hours. It was found that saltwater fish powder was not toxic because there were no dead zebrafish embryos from various concentrations, negative control, and internal control.
Amelogenin and alkaline phosphatase expression in ameloblast after saltwater fish consumption in pregnant mice (Mus musculus) Christiono, Sandy; Pradopo, Seno; Sudiana, I Ketut; Hutami, Islamy Rahma; Mayangsari, Regilia Shinta; Rochmah, Yayun Siti; Ibrahim, Zurairah
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p166-171

Abstract

Background: The intricate process of tooth formation during embryonic development ensures sufficient nutrition for the growth of healthy dental tissues. Amelogenin and alkaline phosphatase (ALP) are serine proteinases secreted by the ameloblast during the transition and maturation phases of the amelogenesis process. Consumption of saltwater fish is predicted to increase the expression of amelogenin and ALP in ameloblast cells during tooth formation. Only now have the function of each gene, tooth-forming cells, and the proteins they produce in the biomolecular amelogenesis of tooth enamel, which began during prenatal development, been clarified. Purpose: This study aims to determine how saltwater fish powder affects the ability of mother mice to increase the expression of amelogenin and ALP in cell ameloblast. Methods: Using a completely randomized design, this study was experimental and aimed to examine the effects of sardine (Sardinella fimbriata), splendid ponyfish (Leiognathus splendens), and tuna (Euthynnus affinis) powder. As samples, twenty-four female mice (Mus musculus) were used. Two groups of mice were created: group 1 (2.14 mg/0.5 ml) and the control group. The expression of amelogenin and ALP was determined using immunohistochemistry (IHC) and t-test (p0.05). Results: Expression of ameloblast was significantly different between the treatment and control groups (p0.05). Conclusion: The consumption of saltwater fish reduces the amelogenin and ALP expressions of mouse fetal ameloblast cells during tooth development in vivo.