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All Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Kefarmasian Indonesia
Al Faizah, Belinda Nabiila
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience

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Green Tea Yoghurt with Encapsulated Lacticaseibacillus paracasei E1 Improves Hepatocyte Damage in High-Fat High-Fructose Diet Mice by Reducing MDA and Increasing SOD Fadlilah, Dawama Nur; Izati, Rahmi; Al Faizah, Belinda Nabiila; Kavitarna, Septhyanti Aprilia; Ardiansyah, Esha; Sa'adah, Nur Alfi Maghfirotus; Atho'illah, Mochammad Fitri; Arifah, Siti Nur; Soewondo, Aris; Jatmiko, Yoga Dwi; Wardhani, Shinta Oktya; Barlianto, Wisnu; Rifa'i, Muhaimin
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.501

Abstract

Background: Obesity is a global epidemic caused by excessive body fat, which is increasing free fatty acids in the liver, causing oxidative stress and liver cell damage. Green tea yogurt (GTY) with encapsulated Lacticaseibacillus paracasei E1 (GTY-LpE1) might have a beneficial effect in reducing liver cell damage. This study was conducted determine GTY-LpE1 effect on superoxide dismutase (SOD) expression, malondialdehyde (MDA) expression and liver histopathology in high-fat high-fructose diet (HFFD) mice. Material and Methods: A completely randomized design (CRD) with 7 groups, including normal diet (ND) group, HFFD group, 1.3 mg/kg BW simvastatin (SIM)-administered HFFD group, 5 g/kg BW probiotic yoghurt (PY)-administered HFFD (PY), 2.5 g/kg BW GTY-administered HFFD (2.5 GTY), 5 g/kg BW GTY-administered HFFD (5 GTY), and 10 g/kg BW GTY-administered HFFD (10 GTY). The diet was given for 16 weeks, followed by oral administration of sim/yoghurt during the last 4 weeks. Mice were sacrificed and the liver was collected. SOD and MDA expression were analyzed by flow cytometry. Histopathology analysis was done by evaluating hematoxylin-eosin (HE) staining of the liver.Result: The percentage of necrotic cells were 34.55, 34.31, and 21.95%, when treated with 2.5, 5, and 10 g/kg BW with GTY-administered HFFD, respectively, these were lower than the ones in the HFFD group (69.49%). The percentage of MDA expression were 15.55, 18.69, and 22.42%, respectively, these were lower than the ones in the HFFD group as well. The percentage of SOD expression were 9.49, 7.85, and 11.11%, respectively, these were higher than the ones in the HFFD group (3.44%). Conclusion: GTY-LpE1 could decrease the number of necrotic cells in the HFFD mice livers and improve the hepatocyte damage by reducing MDA expression and enhancing SOD expression. GTY-LpE1 can be used as an alternative food to control obesity.Keywords: alginate, chitosan, encapsulation, green tea, probiotic
Probiotic-Green Tea Yoghurt on Improving Testicular Histology of High-fat and Fructose Diet Mice Izati, Rahmi; Al Faizah, Belinda Nabiila; Fadlilah, Dawama Nur; Kavitarna, Septhyanti Aprilia; Sa'adah, Nur Alfi Maghfirotus; Ardiansyah, Esha; Atho'illah, Mochammad Fitri; Arifah, Siti Nur; Rifa'i, Muhaimin; Jatmiko, Yoga Dwi; Rahayu, Sri
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 2, AUGUST 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

An unhealthy lifestyle can cause changes in the body's metabolism, leading to obesity. The development of obesity is supported by a disturbance in gut microbiota balance that triggers visceral fat deposition in organs such as the testes. Excess fat deposition triggers inflammation, dysfunction, and high ROS production that can damage testicular tissue. Yoghurt, a fermented milk product fortified with green tea, is high in antioxidants that can help reduce excess ROS. Adding encapsulated probiotics in yoghurt can stabilize the gut microbiota in obesity so that dysbiosis can be resolved. This study was conducted to determine the potential of green tea-probiotic yoghurt (GTY) on testicular tissue repair in mice fed a high-fat and fructose diet (HFFD). The research procedure includes feeding HFFD for 3 months, calculating the Lee index, lactic acid bacteria preparation, microencapsulated probiotics, yoghurt preparation, treatment, data collection, including relative weight of testes, the diameter of seminiferous tubules (DST), the epithelium thickness (ET), the number of Leydig cells (LC), and the number of spermatogenic cells. The mice groups were divided into normal (P0), HFFD (P1), HFFD + simvastatin 1.3 mg/Kg BW (P2), HFFD + plain yoghurt 5 g/Kg BW (P3), HFFD + GTY 2.5 g/Kg BW (P4), HFFD + GTY 5 g/Kg BW (P5), and HFFD + GTY 10 g/Kg BW (P6). The results showed that green tea infusion yoghurt with encapsulated probiotics could improve the structure of testicular tissue in mice after HFFD administration. The most effective dose is green tea yoghurt 5 g/Kg BW.
Co-Authors Ardiansyah, Esha Aris Soewondo Atho'illah, Mochammad Fitri Fadlilah, Dawama Nur Izati, Rahmi Kavitarna, Septhyanti Aprilia Rifa'i, Muhaimin Sa'adah, Nur Alfi Maghfirotus Shinta Oktya Wardhani, Shinta Oktya Siti Nur Arifah, Siti Nur SRI RAHAYU Wisnu Barlianto YOGA DWI JATMIKO