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Niken Niken
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Isolation and Identification of Candida spp in Sputum of Tuberculosis Patients Undergoing Intensive Treatment Suraini Suraini; Anggun Sophia; Niken Niken
Jurnal Kesehatan Medika Saintika Vol 17, No 1 (2026): Juni 2026
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v17i1.30970

Abstract

Tuberculosis is a contagious infectious disease that attacks the lungs and can cause a decline in the immune system, increasing the risk of opportunistic infections, one of which is caused by Candida albicans. This study aimed to isolate and identify Candida albicans in the sputum of tuberculosis patients undergoing intensive treatment. The study used a descriptive laboratory method with 10 sputum samples from tuberculosis patients in the intensive phase. Examination was performed through culture using Sabouraud Dextrose Agar (SDA) media, macroscopic and microscopic observation with lactophenol cotton blue solution, Gram staining, and the Germ Tube test. The results showed that 3 of the 10 samples (30%) were positive for Candida albicans based on colony characteristics, microscopic results, and the Germ Tube test. Two positive samples came from male patients and one sample came from a female patient aged 50–65 years. Based on these results, it can be concluded that opportunistic Candida albicans infections occur in some tuberculosis patients undergoing intensive treatment. Early identification of fungal coinfections is crucial to support successful therapy and prevent further complications in tuberculosis patients.
Isolasi dan Identifikasi Molekuler Candida albicans dari Sekret Keputihan Menggunakan Metode PCR Anggun Sophia; Suraini Suraini; Vetra Susanto; Niken Niken
Jurnal Kesehatan Medika Saintika Vol 16, No 2 (2025): Desember 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i2.30704

Abstract

Candida albicans is one of the major pathogenic fungi responsible for pathological vaginal discharge in women. This study aimed to isolate and identify C. albicans from vaginal discharge samples using conventional methods and molecular confirmation based on Polymerase Chain Reaction (PCR). A total of five vaginal discharge samples were collected and cultured on selective media. The isolation results showed that four samples (SC2–SC5) exhibited colony characteristics consistent with C. albicans, while one sample (SC1) demonstrated morphological features suggestive of the genus Aspergillus. Conventional identification through macroscopic and microscopic examination, as well as the germ tube test, confirmed that the four isolates were strong candidates for C. albicans. Molecular identification using specific primers targeting the Internal Transcribed Spacer (ITS) region indicated that only one isolate produced a distinct DNA band at approximately ±600 bp, which corresponds to the specific ITS fragment size for C. albicans. The SC3 isolate was confirmed as C. albicans based on molecular analysis. These findings highlight the importance of PCR-based confirmation to improve the accuracy of fungal identification, particularly in cases of pathological vaginal discharge.
EKSPLORASI DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA AKTIF EKSTRAK DAUN PALA (Myristica Fragrans) TERHADAP PERTUMBUHAN BAKTERI Streptococcus Mutans PENYEBAB KARIES GIGI SECARA IN VITRO EXPLORATION AND TESTING OF THE ANTIBACTERIAL ACTIVITY OF ACTIVE COMPOUNDS FROM NUTMEG LEAF EXTRACT (Myristica fragrans) ON THE GROWTH OF Streptococcus mutans THE CAUSATIVE AGENT OF DENTAL CARIES IN VITRO Niken Niken; Arniat Christiani Telaumbanua
Jurnal Kesehatan Saintika Meditory Vol 7, No 2 (2024): November 2024
Publisher : STIKES Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jsm.v7i2.2883

Abstract

ABSTRAKKaries merupakan masalah kesehatan gigi yang paling menonjol dengan angka kejadian 96,5%. Berbagai faktor dapat menimbulkan karies gigi salah satunya infeksi bakteri pathogen. Streptococcus mutans (S.mutans) merupakan golongan bakteri yang teradapat dalam rongga mulut manusia yang bersifat kariogenik. Umumnya menangani infeksi bakteri S.mutans dengan pemberian antibiotik. Namun meskipun pemberian antibiotik ini dapat menyembuhkan penyakit keries gigi, penggunaan antibiotik dalam jangka panjang dapat memberikan dampak buruk bagi tubuh. Salah satu upaya pengendalian infeksi bakteri S.mutans yaitu penggunaan bahan alami yang mengandung senyawa antibakteri. Daun Pala (Myristica fragrans) merupakan bahan alami yang memiliki senyawa bioaktif yang bersifat antibakteri. Penelitian ini bertujuan untuk mengeksplorasi senyawa aktif melalui skrinning fitokimia dan melihat aktifitas antibakteri ekstrak daun pala (Myristica fragrans) terhadap pertumbuhan bakteri S.mutans secara in vitro. Penelitian ini merupakan eksperimental laboratorium dengan metode difusi cakram kertas (Kirby-bauer). Sampel yang digunakan adalah bakteri S.mutans. Konsentrasi yang digunakan yaitu 20%, 40%, 60%, 80% , kontrol positif Amoxicilin dan kontrol negatif DMSO. Dalam kesimpulannya, penelitian ini sangat penting dilakukan untuk mengurangi resiko penggunakan antibiotik dalam mengatasi infeksi bakteri pathogen. Hasil penelitian didapatkan daun pala positif mengandung senyawa aktif metabolit sekunder yaitu flavonoid, tannin, saponin dan alkaloid. Hasil uji aktivitas antibakteri menunjukkan pada konsentrasi 20% 16,4 mm (kuat), 40% 17,5 (kuat) mm, 60% 17,6 mm (kuat), 80% 17,8 mm (kuat), kontrol positif (amoxicillin) 17,8 (kuat) dan kontrol negative DMSO 0. Uji ANOVA menunjukkan nilai P.Value = 0,000 (sig.< 0,05) bahwa terdapat perbedaan signifikansi antar semua perlakuan dengan control positif. Disimpulkan bahwa ekstrak daun pala efektif menghambat pertumbuhan bakteri S.mutans. Kata kunci : Streptococcus mutans, Myristica fragrans, Karies Gigi, Fitokimia, Herbal  ABSTRACTDental caries is the most prominent oral health issue, with an incidence rate of 96.5%. Various factors can lead to dental caries, including infections by pathogenic bacteria. Streptococcus mutans (S. mutans) is a type of bacteria found in the human oral cavity that is highly cariogenic. Typically, S. mutans infections are treated with antibiotics. However, while antibiotics can cure dental caries, prolonged use may have adverse effects on the body. One alternative method to control S. mutans infection is using natural substances with antibacterial compounds. Nutmeg leaves (Myristica fragrans) are a natural source of bioactive compounds with antibacterial properties. This study aims to explore active compounds through phytochemical screening and to evaluate the antibacterial activity of nutmeg leaf extract (Myristica fragrans) against S. mutans growth in vitro. This laboratory experiment utilized the disk diffusion method (Kirby-Bauer). The samples used were S. mutans bacteria, with extract concentrations of 20%, 40%, 60%, and 80%, along with positive control (Amoxicillin) and negative control (DMSO). In conclusion, this study is essential in reducing the risks associated with antibiotic use in treating pathogenic bacterial infections. The results showed that nutmeg leaves contained active secondary metabolites, including flavonoids, tannins, saponins, and alkaloids. Antibacterial activity testing revealed inhibition zones of 16.4 mm (strong) at 20%, 17.5 mm (strong) at 40%, 17.6 mm (strong) at 60%, 17.8 mm (strong) at 80%, with 17.8 mm for the positive control (Amoxicillin) and 0 for the negative control (DMSO). ANOVA test results indicated a P-value of 0.000 (sig. < 0.05), confirming significant differences among all treatments compared to the positive control. It was concluded that nutmeg leaf extract is effective in inhibiting the growth of S. mutans.Keywords: Streptococcus mutans, Myristica fragrans, Dental Caries, Phytochemistry, Herbal@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face {font-family:Aptos; panose-1:2 11 0 4 2 2 2 2 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:536871559 3 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:8.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Aptos",sans-serif; mso-ascii-font-family:Aptos; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Aptos; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Aptos; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-font-kerning:1.0pt; mso-ligatures:standardcontextual;}p {mso-style-noshow:yes; mso-style-priority:99; mso-margin-top-alt:auto; margin-right:0cm; mso-margin-bottom-alt:auto; margin-left:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-ascii-font-family:Aptos; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Aptos; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Aptos; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}.MsoPapDefault {mso-style-type:export-only; margin-bottom:8.0pt; line-height:115%;}div.WordSection1 {page:WordSection1;}
Co-Authors -, Suraini Anggun Sophia Arniat Christiani Telaumbanua Vetra Susanto