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Niken Niken
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Isolation and Identification of Candida spp in Sputum of Tuberculosis Patients Undergoing Intensive Treatment Suraini Suraini; Anggun Sophia; Niken Niken
Jurnal Kesehatan Medika Saintika Vol 17, No 1 (2026): Juni 2026
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v17i1.30970

Abstract

Tuberculosis is a contagious infectious disease that attacks the lungs and can cause a decline in the immune system, increasing the risk of opportunistic infections, one of which is caused by Candida albicans. This study aimed to isolate and identify Candida albicans in the sputum of tuberculosis patients undergoing intensive treatment. The study used a descriptive laboratory method with 10 sputum samples from tuberculosis patients in the intensive phase. Examination was performed through culture using Sabouraud Dextrose Agar (SDA) media, macroscopic and microscopic observation with lactophenol cotton blue solution, Gram staining, and the Germ Tube test. The results showed that 3 of the 10 samples (30%) were positive for Candida albicans based on colony characteristics, microscopic results, and the Germ Tube test. Two positive samples came from male patients and one sample came from a female patient aged 50–65 years. Based on these results, it can be concluded that opportunistic Candida albicans infections occur in some tuberculosis patients undergoing intensive treatment. Early identification of fungal coinfections is crucial to support successful therapy and prevent further complications in tuberculosis patients.
Isolasi dan Identifikasi Molekuler Candida albicans dari Sekret Keputihan Menggunakan Metode PCR Anggun Sophia; Suraini Suraini; Vetra Susanto; Niken Niken
Jurnal Kesehatan Medika Saintika Vol 16, No 2 (2025): Desember 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i2.30704

Abstract

Candida albicans is one of the major pathogenic fungi responsible for pathological vaginal discharge in women. This study aimed to isolate and identify C. albicans from vaginal discharge samples using conventional methods and molecular confirmation based on Polymerase Chain Reaction (PCR). A total of five vaginal discharge samples were collected and cultured on selective media. The isolation results showed that four samples (SC2–SC5) exhibited colony characteristics consistent with C. albicans, while one sample (SC1) demonstrated morphological features suggestive of the genus Aspergillus. Conventional identification through macroscopic and microscopic examination, as well as the germ tube test, confirmed that the four isolates were strong candidates for C. albicans. Molecular identification using specific primers targeting the Internal Transcribed Spacer (ITS) region indicated that only one isolate produced a distinct DNA band at approximately ±600 bp, which corresponds to the specific ITS fragment size for C. albicans. The SC3 isolate was confirmed as C. albicans based on molecular analysis. These findings highlight the importance of PCR-based confirmation to improve the accuracy of fungal identification, particularly in cases of pathological vaginal discharge.
Co-Authors -, Suraini Anggun Sophia Vetra Susanto