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THE RELATIONSHIP BETWEEN INTERNAL QUALITY ASSURANCE OF MALARIA PARASITE EXAMINATION WITH THE QUALITY OF MALARIA PREPARATIONS Rita Permata Sari; Vetra Susanto; Fuji Verdian Putra; Endang Suriani; Yustitis Akbar; Meri Wulandari
JURNAL KATALISATOR Vol. 8 No. 1 (2023): Jurnal Katalisator Volume 8 No. 1, April 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i1.2466

Abstract

Internal Quality Assurance of malaria is a mandatory activity carried out by the malaria examination laboratory which includes a whole series of malaria examinations from pre-analytical, analytical, and post-analytical carried out with the aim of obtaining good malaria microscopic examination results in the form of good quality preparations. So far, there has not been much internal quality assurance associated with quality assessment. The purpose of this study was to determine the relationship between internal quality assurance and the assessment of the quality of malaria preparations in the laboratory of RSUD Umbu Rara Meha Waingapu. This type of research is an analytical quantitative approach with a cross sectional design The sample in this study is the total sample. Seven laboratory officers who carried out microscopic examination of malaria were interviewed and observed regarding the implementation of internal quality assurance from the pre-analytical to post-analytical stages, then 23 positive preparations were taken in 2021 and macroscopic and microscopic observations were carried out and an assessment of the quality of malaria preparations was carried out. Statistical analysis using the Pearson test processed using SPSS 25. The results of the study found that the percentage for the implementation of Internal Quality Assurance was 86%, while the percentage of preparate quality was 82%. Based on the Pearson correlation test between the two variables of internal quality assurance and also the quality of malaria preparate obtained a p-value of 0.010 where p < 0.05 which means there is a relationship between internal quality assurance and the quality of malaria preparate.  
SUGAR LEVELS AND PROTEIN LEVELS OF URINE SAMPLES DECREASED DUE TO THE DELAY OF EXAMINATION OF 1 HOUR AND 2 HOURS: KADAR GULA DAN KADAR PROTEIN SAMPEL URIN MENURUN KARENA TERTUNDANYA PEMERIKSAAN 1 JAM 2 JAM vetra susanto; Rita Permata Sari; Endang Suriani; Sri Suciana
JURNAL KATALISATOR Vol. 8 No. 1 (2023): Jurnal Katalisator Volume 8 No. 1, April 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i1.2542

Abstract

The sugar levels and protein levels in a urine sample may change over time after sampling. Such delays can affect the stability or degradation of components in the urine, including sugar and protein. Several studies have been conducted concerning that delay such as the difference in the length of time of the delay. But the information that the delay affects the sugar content and protein content of the sample, has not been studied much. The purpose of the study was to analyze the sugar levels and protein levels of urine samples that experienced delayed examination. This study was a laboratory study, with the sample being urine. Because of this, a clearance ethics test was carried out. Chemical Analysis of urine is the measurement of chemical components in urine, namely sugar (glucose), protein, uric acid, ketones, bilirubin, nitrite, and urine pH. Chemical analysis can help in the diagnosis of certain diseases or conditions, such as diabetes, kidney disease, urinary tract infections, or liver disorders. The result was that urine levels and protein levels dropped after a delay in examination for 1 hour and 2 hours. Postponement of the examination lowers the sugar level and protein content of the urine sample. By understanding the changes in sugar and protein levels that can occur during delayed urine examinations, healthcare practitioners can make more informed decisions in the diagnosis and management of patients.
UJI PERBANDINGAN EFEKTIFITAS REBUSAN DAUN KEMANGI (Oncimum Basilicum) DAN DAUN SIRSAK (Annona Muricata L) TERHADAP KEMATIAN LARVA NYAMUK AEDES AEGYPTI Vetra Susanto; Endang Suriani; Dian Dilastri Putri
Jurnal Kesehatan Medika Saintika Vol 17, No 1 (2026): Juni 2026
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v17i1.30971

Abstract

Dengue Hemorrhagic Fever (DHF) is still a health problem in Indonesia and is transmitted through the bite of the Aedes aegypti mosquito. Continuous use of chemical larvicides can cause resistance and environmental pollution, so alternatives to natural larvicides are needed, such as basil leaves (Ocimum basilicum) and soursop leaves (Annona muricata L.). This study aims to compare the effectiveness of boiled basil and soursop leaves on the death of third instar Aedes aegypti larvae. The research used a laboratory experimental method with a completely randomized design (CRD). The concentrations used were 25%, 30%, 35% and 40% with three repetitions and observations for 24 hours. Data analysis was carried out using normality, homogeneity, One Way ANOVA, Duncan test, and probit tests to determine LC50. The results showed that the percentage of larval death in boiled basil leaves was 23.3%; 36.6%; 40%; and 60%, while soursop leaf decoction is 70%; 76.6%; 76.6%; and 86.6%. One Way ANOVA test showed that there were significant differences between treatments (p < 0.05). Decoction of soursop leaves is more effective than decoction of basil leaves as a natural larvicide against Aedes aegypti larvae.
Isolasi dan Identifikasi Molekuler Candida albicans dari Sekret Keputihan Menggunakan Metode PCR Anggun Sophia; Suraini Suraini; Vetra Susanto; Niken Niken
Jurnal Kesehatan Medika Saintika Vol 16, No 2 (2025): Desember 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i2.30704

Abstract

Candida albicans is one of the major pathogenic fungi responsible for pathological vaginal discharge in women. This study aimed to isolate and identify C. albicans from vaginal discharge samples using conventional methods and molecular confirmation based on Polymerase Chain Reaction (PCR). A total of five vaginal discharge samples were collected and cultured on selective media. The isolation results showed that four samples (SC2–SC5) exhibited colony characteristics consistent with C. albicans, while one sample (SC1) demonstrated morphological features suggestive of the genus Aspergillus. Conventional identification through macroscopic and microscopic examination, as well as the germ tube test, confirmed that the four isolates were strong candidates for C. albicans. Molecular identification using specific primers targeting the Internal Transcribed Spacer (ITS) region indicated that only one isolate produced a distinct DNA band at approximately ±600 bp, which corresponds to the specific ITS fragment size for C. albicans. The SC3 isolate was confirmed as C. albicans based on molecular analysis. These findings highlight the importance of PCR-based confirmation to improve the accuracy of fungal identification, particularly in cases of pathological vaginal discharge.