Claim Missing Document
Check
Articles

Found 29 Documents
Search

Administration of Probiotics in Recirculating System Cultivation to Improve the Health of Colossoma macropomum Nurilam Sinaga; Morina Riauwaty; Iesje Lukistyowati
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.101-110

Abstract

Probiotic containing Nitrosomonas sp and Nitrobacter sp play a role in the nitrification process to improve water quality in the media maintenance. This research was conducted in July – October 2021 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University. The purpose of this study was to obtain the best dose of Kp Super N probiotics for fish health. The method used is an experimental method using a one-factor with five treatment levels and three replications. The treatments applied were Cn (maintenance without probiotic Kp Super N and without being challenged with Aeromonas hydrophila), Cp (maintenance without probiotic Kp Super N and challenged with A. hydrophila), maintenance was given probiotic Kp Super N and challenged with A. hydrophila: P1 (2 mL/80 L of water), P2 (3 mL/80 L), P3 (4 mL/80 L). Fish were reared for 72 days. The results shown that the best treatment was P3 (4 mL/80 L) with a total erythrocyte of 2,70 x 106 cells/mm3, haematocrit value of 36,00%, and hemoglobin level of 8,17 g/dl, total leukocytes 8,17 x104 cells/mm3, leukocrite 3,17%, lymphocytes 63,00%, monocytes 4,00%, neutrophils 1,00%, and platelets 32,00%. Weight growth was 23,29 g and the survival rate 100%. The water quality temperature 26 – 29,7oC, pH 6 – 7,5, DO 4,9 – 5,2 ppm, ammonia 0,05430-0,00062 mg/L, nitrite 0,0549-0,1709 mg/L, and nitrate 0,2000-3,6670 mg/L. Based on the date obtain, it can be concluded that the probiotic Kp Super N is able to improve the health of Colossoma macropomum
Hematology of Osphronemus goramy Fed with Fermented Vegetable Waste with Cows Rumen Ade Hidayat Ilka; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.111-125

Abstract

Vegetables in chicory and cabbage contain flavonoid compounds, alkaloids, saponins, glycosides, vitamin C, glucosinolates, glutamine, and sulforaphane, which have antimicrobial activity that can be used as immunomodulators that can strengthen the immune system. This research was conducted from February to April 2022 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to analyze the effect of fermenting mustard and cabbage waste flour in artificial feed on goramy hematology. The method used is an experimental method using a one-factor Completely Randomized Design (CRD) with five treatment levels and three replications. The treatments were P0 (Feed without added fermented chicory and cabbage and challenge with Aeromonas hydrophila), and fish were fed with fermented chicory and cabbage with doses of P1 (15%), P2 (25%), P3 (35%), P4 (45%) and challenged with A. hydrophila. Fishes were reared for 60 days with feed containing fermented chicory and cabbage, and the final data was 14 days of testing with A. hydrophila with the same feed. The results showed that the best dose of feed containing fermented chicory and cabbage was in treatment P2 (25%) with a total erythrocyte of 2.21 x106 cells/mm3, a hemoglobin level of 5.40 g/dL, and a hematocrit value of 38.67%. Tested with A. hydrophila bacteria, total erythrocytes 2.68 x106 cells/mm3, hemoglobin 6.60 g/dL, hematocrit value 39.00%, survival rate 96, 67%, absolute weight growth 1.06 g. Data obtained on O. goramy fed with fermented vegetable waste with cows' rumen showed an effect on improving fish hematology.
Leukocytes Profil of Monopterus albus raired in Biofloc System with Different Stock Densities Nurkholis Ahmad; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.137-145

Abstract

Biofloc is an agglomerate composed of various autotrophic and heterotrophic organisms as well as particles, which are mixed by aeration and circulation to form clumps that integrate quite well in water. This research was carried out in March- May 2021 in the Hatcheries and Experimental Ponds and Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The study aimed to analyze the leukocyte profile of the Monopterus albus raired biofloc system with different stock densities. The research method used a one-factor, completely randomized design (CRD) with four treatments with three replications. The treatments applied were P1 (of 10 fish/25 L), P2 (of 20 fish/25 L), P3 (of 30 fish/25 L), and P4 (of 40 fish/25 L). M. albus were reared in a biofloc system for 40 days. The results showed that there was an effect of stock densities on the leukocyte profile of M. albus reared in biofloc systems with different stock densities, a significant effect (P<0.05). The best stock density was at P3 (30 fish/25 L), where the total leukocytes were 3.62 x 104 cells/mm3, leucocrite was 2.00%, phagocyte activity was 36.66%, while the percentage of lymphocytes was 83.00 %, monocytes was 16.00%, and neutrophils was 13.00%. Water quality during this study was at temperature 26-300C, pH 6-7, dissolved oxygen 4.4-6.8 mg/L, and ammonia 0.02-0.06 mg/L. It was concluded that there was an effect on the biofloc system of stock density on the leucocyte profile M. albus
Leukocytes Differentiation of Oreochromis niloticus Fed with Herbs Fermented and Challenged with Aeromonas hydrophila Annisa Dwi Putri; Morina Riauwaty; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.146-154

Abstract

Herb-fermented medicine consists of Curcuma zanthorrhiza, Curcuma domestica Val. and Kaempferia galanga, which contains active substances to overcome disease. This research was conducted from August to December 2021 at the Marine Science Biotechnology Laboratory and Fish Parasite and Disease Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The method used in this study was an experiment using a Complete Randomized Design (CRD), one factor with five treatments and three replications. The treatment of this study was Kn: Negative control (feeding without herbs fermented and without being challenged Aeromonas hydrophila), Kp: Positive Control (feeding without herbs fermented and challenged with A. hydrophila), P1; P2 and P3: Fed herbs fermented at a dose of 150, 175 and 200 mL/kg and challenged with A. hydrophila. The fish used was 6-7 BW with a 1 fish/4L density. The result showed the best dose was in P3, with a total of leukocytes 5,01x104 cells/mm3, Lymphocytes 77,67%, Neutrophils 7,67%, Monocytes 14,67%, Phagocytic activity 37,67%. Based on the data obtained, it can be concluded that fermented herbs have a significant effect on the leukocyte differentiation of Oreochromis niloticus
Histopathology of Intestine and Liver of Oreochromis niloticus Fed with Herbs Fermented and Infected with Aeromonas hydrophila Dwi Indah Fransiska; Morina Riauwaty; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.175-183

Abstract

Natural ingredients used in the manufacture of fermented herbs medicine, such as turmeric, ginger, and kencur. These plants are antibacterial and antimicrobial and increase the growth of fish. This research was conducted from November 2021 to March 2022 at the Faculty of Fisheries and Marine, Riau University. The purpose of this study was to analyze the histopathology of the intestine and liver of Oreochromis niloticus fed with herbs fermented pellet and infected with Aeromonas hydrophila. The method used is an experiment with a completely randomized design (CRD) one factor, five levels of treatment, and three replications so that 15 experimental units were obtained. The treatments used were Kn (feeding without fermented herbs and without infection with A. hydrophila bacteria), Kp (feeding without fermented herbs and infected with A. hydrophila bacteria) and feeding with the addition of fermented herbs and infected with bacteria A. hydrophila at a dose of 150 mL/kg (P1), 175 mL/kg (P2), and 200 mL/kg (P3). The results showed that the damage that occurs in the intestine was edema, haemorrhage and necrosis. Liver damage in the form of hypertrophy, bleeding, vacuole degeneration and necrosis. The best dose was found which is 200 mL/kg of feed (P3). The survival rate was 85,00%. It can be concluded that the addition of herbs fermented to feed can improve the structure of O.niloticus
Antibacterial Activity Test of Cinnamomum burmannii Solution in Inhibiting the Growth of Aeromonas hydrophila Bacteria Lilis Cahaya Septiana; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 5 No. 1 (2024): May
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.1.1-10

Abstract

Aeromonas hydrophila bacteria that cause MAS (Motile Aeromonas Septicemia) disease. Cinnamon contains essential oils, tannins, saponins, flavonoids and phenols. The active compounds of cinnamon essential oil were cinnamaldehyde and cinnamic acid that can inhibit the growth of bacteria. The research was carried out from March to July 2022 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau, Pekanbaru. This study aimed to determine the sensitivity of Cinnamomum burmannii in inhibiting the growth of Aeromonas hydrophila bacteria, Minimum Inhibitory Concentration (MIC), and Lethal Dose (LD50) concentration of C. burmannii solution against Cyprinus carpio by immersion technique for 24 hours. The research method used was an experimental method using the Kirby Bauer disc method with concentrations of 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, and 1%. The results shown that the sensitivity of the C. burmannii solution to A. hydrophila bacteria ranged from 22.60 mm to 6.67 mm. The MIC value at a concentration of 4% was determined as the average number of bacterial colonies 143 CFU/mL. The LD50 value of C. burmannii solution against Cyprinus carpio by immersion for 24 hours was 3.64%. Based on the data abtain, it can be concluded that C.burmannii is able to inhibit the growth of A.hydrophila bacteria.
Identification of Clinostomum sp. Parasites In Swamp Sepat Fish (Trichogaster trichopterus) In FAPERIKA DAM, Pekanbaru Ahmad Tirmizi Nasution; Iesje Lukistyowati; Morina Riauwaty
Jurnal Akuakultur SEBATIN Vol. 4 No. 1 (2023): May
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.1.49-57

Abstract

Parasites are organisms that live on other organisms that can benefit from the symbiosis, while the host is harmed. This research was conducted from March to Mei 2022 at the Laboratory of Fish Parasites and Diseases, Department of Aquaculture, Faculty of Fisheries and Marine. Trichogaster trichopterus sampling was carried out Feperika Dam, Pekanbaru. The aim of the study was to obtain the prevalence and intensity of the parasite Clinostomum sp. which infects the swamp fish (Trichogaster trichopterus) in Faperika Dam, Pekanbaru. The method used is a survey method and direct observation of the sample. Sampling was carried out three times with sampling points consisting of 3 places, namely: Location 1 is at the entrance of the reservoir, locations 2 and 3 are located to the west and north of location 1 with a distance of each location 50 meters. The results of the identification showed that the sample of bog fish infected with trematode parasites was Clinostomum sp. with prevalence ranging from 93.9-100%, prevalence in locations 1 and 2 (100%), and intensity for each location, namely, at location 1 at 36.9 ind/bird, location 2 at 41.3 ind and location 3 is 34.3 ind.
Differentiation of Leukocytes of Dumbo Catfish (Clarias gariepinus) Infected with Aeromonas hydrophila and Treated with Bay Leaf Solution (Syzygium polyantha) Tomuso Angdi Suryani Sinaga; Morina Riauwaty; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 4 No. 1 (2023): May
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.1.58-70

Abstract

The research was conducted from January to October 2021 at the Laboratory of Fish Parasites and Diseases, Departement of Aquaculture, Faculty of Fisheries and Marine, University of Riau. The purpose of this research was to analyze and obtain the best dose of bay leaf solution to treat catfish (Clarias gariepinus) infected with Aeromonas hydrophilla. Bay leaf is a plant that has antibacterial properties in fish because it contains secondary metabolites, namely tannins, saponins, steroids, alkaloids, flavonoids, and essential oils. The method used is an experimental method with a Completely Randomized Design (CRD), one factor with five treatments. The treatment applied is Kn (without immersed with bay leaf solution and not infected by A. hydrophila), Kp (without immersed with bay leaf solution and infected by A. hydrophila), P1 (1000 ppm), P2 (1100 ppm), P3 (1200 ppm). The curing is done by immersed with bay leaf solution for 5 minutes, carried out three times with an interval of 24 hours and then maintained for 14 days after treatment. The result shown that bay leaf solution able to cure catfish infected by A. hydrophila. The best dose was 1200 ppm with average total leukocytes of 12.61 × 104 cells /mm3, leukocyte differentiation (lymphocytes 84.66%, Neutrophils 6.66%, Monocytes 8.66%), phagocytitosis activity is 35.33%, and the survival rate 96.67%.
The Effect of Soaking Jambal Siam Fish (Pangasianodon hypophthalmus) In Pineapple Peel (Ananas comosus) Solution On Leukocyte Image Herparinda Novita; Morina Riauwaty; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 4 No. 1 (2023): May
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.1.78-89

Abstract

This research was carried out in July 2021 – September 2021 at the Laboratory of Parasites and Fish Diseases. This study aims to obtain the best dose of pineapple peel solution. This study used a Complete Randomized Design (RAL) consisting of 5 treatments and 3 tests. The treatment in this study was that there was an influence where the best treatment was found in P2 (Soaking of pineapple skin solution dose 1,800 ppm tested challenged with A. hydrophila bacteria) with an average total leukocytes of 9.92 x 104 cells / mm3, total lymphocytes 82.67%, total monocytes 9.33%, neutrophy value 8.00% phagocytic value 37.00%, siamese jambal fish dilution classified as good with a value of 93.33%, and water quality during the study temperatures ranged from 27.3–29oC,, pH 6.3–6.6, DO 3.4–4.2 mg/L, and NH3 0.01-0.02 mg/L.