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Anak Agung Ayu Niti Wedayani
Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Effect of Formaldehyde Inhalation on Leukocyte Counts and Malondialdehyde Levels in Wistar Rats (Rattus norvegicus) Muhammad Fauzan; Ima Arum Lestarini; Anak Agung Ayu Niti Wedayani
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6087

Abstract

Formaldehyde is a chemical compound that is often found in everyday life, such as in the chemical industry to research laboratories. Formaldehyde has toxic properties that can harm humans in long term exposure. The genotoxic and cytotoxic properties of formaldehyde can cause oxidative stress. This study assesses the number of leukocytes and levels of malondialdehyde in Wistar rats induced by 40 ppm formaldehyde. This study used 12 Wistar rats taken at random and put into 2 groups, namely the control and treatment groups. The treatment group was given formaldehyde inhalation at a concentration of 40 ppm for 16 weeks. At the 16th week, rat blood was taken retroorbital to calculate the number of leukocytes and levels of malondialdehyde. The calculation results were then tested statistically using IBM SPSS Statistics 25 software. The results showed that there was no significant difference (p>0.05) between the number of leukocytes in the control group and the treatment group. This study also showed that there was a significant difference (p<0.05) between malondialdehyde levels in the control group and the treatment group. In conclusion, there was no effect of formaldehyde induction with a concentration of 40 ppm on the number of leukocytes and there was an effect of formaldehyde induction with a concentration of 40 ppm on malondialdehyde levels in Wistar rats.
Effectiveness of Cages Inducing Nasopharyngeal Dysplasia in Wistar Rats (Rattus norvegicus) Gede Rama Hardy Nugraha; Hamsu Kadriyan; Anak Agung Ayu Niti Wedayani; Eka Arie Yuliyani
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6088

Abstract

The cage is a place used to put experimental animals in experimental studies to study the mechanism of the disease and its response to therapy. Formaldehyde as a carcinogenic compound can increase the occurrence of chronic inflammation which has the potential to increase the growth of cancer cells in experimental animals due to the stress experienced while in the cage. Therefore, the purpose of this study was to compare the effectiveness of large and small cages induced by formaldehyde 30 ppm against nasopharyngeal dysplasia in Wistar rats. This research is an experimental study with a true experimental design with the type of post-test only control group design. This study used 12 male Wistar rats (Rattus norvegicus) which were divided into two groups, namely the group of Wistar rats which were placed in large cages measuring (30 x 24 x 11.5) cm3 of 6 individuals, and in small cages (27 .5 x 21.5 x 9) cm3 of 6 individuals induced by 30 ppm formaldehyde with a span of 6 hours per day for 16 weeks. The rats were terminated and retro-orbital blood samples were taken to measure MDA levels and nasopharyngeal tissue to assess the degree of dysplasia. The results of this study showed that there was a statistically significant difference (p <0.05) between the degrees of nasopharyngeal dysplasia (p=0.003) and MDA levels (p=0.003) in Wistar rats in small cages and large cages. In this study the effectiveness of small cages was greater than large cages in causing dysplasia in the nasopharyngeal tissue of Wistar rats.
The Relationship Between The Total Count of Leukocyte Types and Formahldehyde Induction in Wistar Rats Anak Agung Bagus Tito Indra Prawira Negara; Anak Agung Ayu Niti Wedayani; Bayu Tirta Dirja
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6089

Abstract

Formaldehyde is a chemical compound with a high level of toxicity. In the industrial sector, formaldehyde is commonly found in cosmetic products, resins, adhesives, plastics, and wood products. Therefore, the objective of this research is to determine the effect of formaldehyde induction with concentrations of 20 ppm, 30 ppm, and 40 ppm on the total count of leukocyte types. This study is a qualitative research with a true experimental design employing a post-test only control group design. The study used male Wistar rats (Rattus norvegicus) totaling 24 individuals, which were divided into 4 groups: a control group and 3 treatment groups. Each group consisted of 6 rats induced with formaldehyde at concentrations of 20 ppm, 30 ppm, and 40 ppm. The Wistar rats will be terminated, and retro-orbital blood sampling will be conducted to count the various types of leukocytes in order to determine whether there are differences in leukocyte type counts among rats induced with formaldehyde. Data analysis will employ the Kruskal-Wallis test. The results of this study showed that there was a statistically significant difference (p <0.05) between formaldehyde and the total count of leukocyte types. In this study, it was found that there is a decrease in the total count of leukocyte types in the formaldehyde-exposed groups at 40 ppm, 30 ppm, and 20 ppm when compared to the control group.
The Effect of Feeding Non-Antioxidant Feed on The Degree of Dysplasia in Formaldehyde-Induced Wistar Rats Ni Putu Ayu Nindya Dewi Cahya Putri; Anak Agung Ayu Niti Wedayani; Ima Arum Lestarini; Abdul Qadar Punagi; Nova Audrey Luetta Pieter; Muhammad Husni Cangara; Novia Andansari Putri Restuningdyah
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6091

Abstract

Nasopharyngeal dysplasia is a condition of abnormal cellular growth that occurs due to the failure of nasopharyngeal epithelial cells in carrying out cellular adaptation. This condition can occur due to the continuous inhalation of toxic compounds, such as formaldehyde, which has genotoxic and cytotoxic effects that can induce cellular damage. One of the compound activity associated with reducing risk of dysplasia is antioxidant activity. Therefore, it is important to know whether the presence of antioxidant activity affects the occurrence of dysplasia. This study is an experimental study with a post-test only control group design which aims to determine the effect of Non-Antioxidant Feed on the occurrence of nasopharyngeal dysplasia. Testing of the antioxidant activity in feed was carried out using the DPPH assay. There were two groups of experimental animals including the treatment group (fed with Non-Antioxidant Feed) and the control group (fed with SB 11). All animals in each group were induced with 40 ppm formaldehyde by inhalation for 8 weeks and then euthanized using chloroform inhalation for nasopharyngeal tissue extraction. The results of the histopathological examination of the two groups were then statistically tested using the Mann-Whitney U test and obtained a value of P<0.05 (P=0.006), which means that there are effect of feeding Non-Antioxidants Feed on the degree of dysplasia in formaldehyde-induced wistar rats. The results can be associated with the ingredients contained in the feed given, differences in genetic polymorphisms of each subjects, and effect of body weight variations of the rats caused by randomization.
Co-Authors Abdul Qadar Punagi Anak Agung Bagus Tito Indra Prawira Negara Bayu Tirta Dirja Eka Arie Yuliyani Gede Rama Hardy Nugraha Hamsu Kadriyan Ima Arum Lestarini Muhammad Fauzan Muhammad Husni Cangara Ni Putu Ayu Nindya Dewi Cahya Putri Nova Audrey Luetta Pieter Novia Andansari Putri Restuningdyah