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The Effect of Adding White Tumeric (Curcuma zedoaria) Extract on the Immune System of Vaname Shrimp (Litopenaeus vannamei) Tested Against Vibrio harveyi Bacteria Rosyida, Azilia; Setyowati, Dewi Nur’aeni; Azhar, Fariq
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The entry of vaname shrimp into Indonesia was to replace tiger prawns (Penaeus monodon) which at that time experienced a decline in quality. Although vaname shrimp is famous for having various advantages, there are still obstacles encountered during the cultivation period, one of which is the attack of vibriosis caused by the bacterium Vibrio harveyi. This study aims to determine the effect of adding white turmeric extract (Curcuma zedoaria) on the immune system of white shrimp (Litopenaeus vannamei) which was challenged with Vibrio harveyi bacteria. This research was conducted for 60 days from March to May 2021 using experimental research methods. The research container used a container container (size 40x30x28 cm) and a stocking density of 20 fish/container with a volume of 20 L. The research design used was a completely randomized design consisting of 5 treatments with 3 replications where P1: artificial feed + Vibrio harveyi infection, P2: artificial feed + 0.1 mL NaCl injection, P3: artificial feed + 0,5% white turmeric extract + V.harveyi infection, P4: Artificial Feed + 1% white turmeric extract + V.harveyi infection, P5: Artificial feed + 2% white turmeric extract + V.harveyi infection. The results showed that administration of white turmeric extract (C.zedoaria) with a dose of 1% gave the best results and had a significant effect on the survival rate of 68.3%, Total Haemocyte Count of 21.63x10⁶ cells/mL, and Differential Haemocyte Count (hyaline cells). by 58.7%, semi-granulocytes by 18.33%, granulocytes by 23%) which play a role in the immune system of white vaname shrimp. In addition, the value of phagocytic activity was 68.8% and was able to suppress the growth of bacteria in the intestine at the lowest at 57.7×108 CFU/mL and suppress the number of vibrios to the lowest number at 26×106 CFU/mL.
EFEKTIVITAS EKSTRAK DAUN KAWISTA (Limonia acidissima) TERHADAP SISTEM IMUN IKAN NILA (Oreochromis niloticus) YANG DIINJEKSI BAKTERI Aeromonas hydrophila Febriana, Nurlita; Azhar, Fariq; Abidin, Zaenal; Setyowati, Dewi Nur’aeni
Jurnal Media Akuakultur Indonesia Vol 4 No 4 (2024): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v4i4.6139

Abstract

ABSTRACT Tilapia (Oreochromis niloticus) is a freshwater commodity that has high prospects in the fisheries sector. A. hydrophila bacteria is a pathogenic bacteria that can cause death, often found in freshwater fish. The kawista plant contains alkaloids, tannins, flavonoids, steroids and polyphenols. The objective of the study was to determine the effectiveness of kawista leaf extract Limonia acidissima) against the immune system of tilapia (Oreochromis niloticus) injected with A. hydrophila bacteria. This study was experimental using a completely randomized design (RAL) method consisting of 5 treatments with 3 replications namely K- (Not given kawista leaf extract and injected with 0.9% NaCl), K+ (Not given kawista leaf extract and injected with A. Hydrophila bacteria ), P1 (Addition of kawista leaf extract to feed with a dose of 0.5% and injection of A. hydrophila bacteria, P2 (Addition of kawista leaf extract to feed with a dose of 1% and injection of A. Hydrophila bacteria), P3 (Addition of kawista leaf extract to feed with dose of 2% and injection of A. Hydrophila bacteria).The results showed that giving kawista leaf extract with different doses affected the immune system in tilapia and fish survival.In this study the best treatment was obtained in treatment 2 with a total erythrocyte value of 1.92 x106 cell/mm3, total leukocytes 2.58x104 cell/mm3, differential leukocytes are divided into 4 parts, namely lymphocytes 66.7%, monocytes 5.33%, neutrophils 21.7%, and platelets 6.33%, hemoglobin 6.6%, hematocrit 17.33%, phagocytosis activity 69.93%, and a Total Bacteria Count (TBC) of OD620 3.53 and a survival rate of 68%. The conclusion was obtained that the use of kawista leaf extract at a dose of 1% in feed could improve the immune system of tilapia injected with A. hydrophila bacteria.
Efektivitas Ekstrak Daun Komak (Lablab purpureus) Terhadap Sistem Imun Ikan Nila (Oreochromis niloticus) Yang Diinjeksi Bakteri Aeromonas hydrophila Rabi’atussamiah; Azhar, Fariq; Abidin, Zaenal; setyowati, Dewi Nur’aeni
Jurnal Media Akuakultur Indonesia Vol 4 No 4 (2024): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v4i4.6140

Abstract

This study aims to determine the effect of comma leaf extract on the immune system of tilapia injected with A. hydrophila bacteria. This research was conducted for 60 days at the Fish Production and Reproduction Laboratory and Aquaculture Fish Health Laboratory, Aquaculture Study Program, Department of Fisheries and Marine Sciences, Faculty of Agriculture, University of Mataram. The method used was a completely randomized design (CRD) experiment with five treatments and three replications, namely where K- was not given comma leaf extract and was injected with 0.9% NaCl, K+ was not given comma leaf extract and was injected with A. Hydrophila bacteria, P1 (0.5%) extract comma leaf and injected with A. hydrophila bacteria, P2 (1%) of komak leaf extract and injected with A. hydophila bacteria, P3 (2%) of komak leaf extract and injected with A. hydrophila bacteria. The observed parameters were total erythrocytes, total leukocytes, hemoglobin, hematocrit, differential leukocytes, phagocytosis activity, total bacteria and survival (SR). The results showed that the average value of total erythrocytes was 1.22-1.82 x106 cells/mm3, total leukocytes were 2.08-2.8 x104 cells/mm3, hemoglobin was 3.4-5.5%, hematocrit was 14.1-17.3%, differential leukocytes consisted of 4 types of cells, namely lymphocytes. , monocytes, neutrophils and platelets. Lymphocytes 60.7-72.3%, monocytes 4.3-7.3%, neutrophils 17-24.3%, and platelets 6.3-7.7%, phagocytosis activity 44.8-66.3%, total bacteria (TB) OD620 3.39-3.84, survival (SR) 46.7-66.7 %. The addition of 2% of comma leaf extract to the feed can improve the immune system of tilapia injected with A. hydrophila bacteria
The Effect of Adding White Tumeric (Curcuma zedoaria) Extract on the Immune System of Vaname Shrimp (Litopenaeus vannamei) Tested Against Vibrio harveyi Bacteria Rosyida, Azilia; Setyowati, Dewi Nur’aeni; Azhar, Fariq
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The entry of vaname shrimp into Indonesia was to replace tiger prawns (Penaeus monodon) which at that time experienced a decline in quality. Although vaname shrimp is famous for having various advantages, there are still obstacles encountered during the cultivation period, one of which is the attack of vibriosis caused by the bacterium Vibrio harveyi. This study aims to determine the effect of adding white turmeric extract (Curcuma zedoaria) on the immune system of white shrimp (Litopenaeus vannamei) which was challenged with Vibrio harveyi bacteria. This research was conducted for 60 days from March to May 2021 using experimental research methods. The research container used a container container (size 40x30x28 cm) and a stocking density of 20 fish/container with a volume of 20 L. The research design used was a completely randomized design consisting of 5 treatments with 3 replications where P1: artificial feed + Vibrio harveyi infection, P2: artificial feed + 0.1 mL NaCl injection, P3: artificial feed + 0,5% white turmeric extract + V.harveyi infection, P4: Artificial Feed + 1% white turmeric extract + V.harveyi infection, P5: Artificial feed + 2% white turmeric extract + V.harveyi infection. The results showed that administration of white turmeric extract (C.zedoaria) with a dose of 1% gave the best results and had a significant effect on the survival rate of 68.3%, Total Haemocyte Count of 21.63x10⁶ cells/mL, and Differential Haemocyte Count (hyaline cells). by 58.7%, semi-granulocytes by 18.33%, granulocytes by 23%) which play a role in the immune system of white vaname shrimp. In addition, the value of phagocytic activity was 68.8% and was able to suppress the growth of bacteria in the intestine at the lowest at 57.7×108 CFU/mL and suppress the number of vibrios to the lowest number at 26×106 CFU/mL.