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Uji Sitotoksik Fraksi n-Heksana, Etil Asetat, Etanol dari Ekstrak Etanolik Daun Ende (Coccinia grandis (L.) Voigt) pada Sel HeLa NURAINI HARMASTUTI; GUNAWAN PAMUDJI WIDODO; MARIA E.D.O. HUREK MAKING; MARIA YANGSYE LENGGU; FEOLISTIN MIENCE POBAS
Jurnal Farmasi Indonesia Vol 9 No 1 (2012): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1009.178 KB) | DOI: 10.31001/jfi.v9i1.725

Abstract

Ende leaf (Coccinia grandis (L.) Voigt) is the one of plant that most popular in Nusa Tenggara Timur province. The study was aimed to know the cytotoxic effect of n-hexane, ethyl acetate, and ethanol fractions from ethanolic extract of ende leaf on hela cell and its IC50 value. Dried ende was macerated with 96 % ethanol solvent, then it was fractionated by n-hexane, ethyl acetate, and ethanol solvent. Then it was tested the cytotoxic by MTT (Microculture Tetrazolium Salt) method on HeLa cell The absorbance response was converted into abbreviation of cell viability. IC50 value was calculated with linear regression equation between logarithm of concentration samples versus percentage viability of HeLa cell. The result showed that the cytotoxic test of ende leaf against HeLa cell presented the IC50 value of ethanolic extract and n-hexane, ethyl acetate, and ethanol fractions were 415.719 ppm; 307.142 ppm; 50303.71 ppm; dan 50722.424 ppm, respectively.
Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Pohon Faloak (Sterculia sp.) Terhadap Bakteri Staphylococcus Aureus Priska Ernestina Tenda; Maria Yangsye Lenggu; Marini Sriyuni Ngale
JURNAL INFO KESEHATAN Vol 15 No 1 (2017): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

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Abstract

Faloak is a medicinal plant that grows in extreme conditions in the East Nusa Tenggara region. Faloak has benefits for herbal treatment wherein the content of saponin compounds, steroids and triterpenoids, flavonoids, and alkaloids found in the bark of the faloak tree serves for the treatment of various diseases caused by bacteria. The purpose of this research is to know the antibacterial activity of ethanol extract of faloak bark (Sterculia sp.) On the growth of Staphylococcus aureus bacteria. Determination of antibacterial activity by a diffusion method using a cylinder, and see the existence of a clear zone around a cylinder. The results showed that the concentration of 22.5% w / v; 45% w / v; 75% w / v and 100% w / v were able to inhibit the growth of Staphylococcus aureus bacteria with mean inhibitory zone diameter of 1.33 cm, 1.66 cm, 1.90 cm, 2.13 cm, the concentration of the inhibitory zone is greater so that the effective concentration inhibiting the growth of Staphylococcus aureus bacteria is 100% w / v.
Kajian Perilaku Swamedikasi Menggunakan Obat Penggemuk Badan Oleh Pasien Pengunjung Apotek Di Kota Kupang Tahun 2016 Maria Yangsye Lenggu
JURNAL INFO KESEHATAN Vol 15 No 2 (2017): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

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Abstract

Community efforts to treat themselves are known as self-medication. Self-medication is usually done to treat complaints and minor ailments. The Sudan Journal of Rational Use of Medicine (2014) found 7 categories of drugs most widely used in self-medication, one of which is a body fat drug. Self-medication acts tend to increase. Like whether self-medication behavior uses body fat drug performed by pharmacy visitors in Kupang city? The purpose of this research is to determine the prevalence, behavior, and perception of self-medication patients using body fat drug. This research is a descriptive observational study with the subject inclusion criteria. Pharmacy visitor patients who buy body fat medicine for self-medication. The study was conducted in August - October 2016. Data collection tools were questionnaires with 60 questions covering 37 Likert scale questions distributed in 10 pharmacies. The questionnaire returned was 108. The study of self-medication behavior with prevalence indicators was 48.15% had purchased/used the drug in the past 1 month, 32.41% self-medication for me, 21.30% 2 times/ treatment period, 35.19% used dexamethasone drugs, livron B plex and pronicy. Study with behavioral indicators, as much as 62.04% of respondents knew the body fat drug, 37.96% of respondents got drug information from friends, 45.37% bought at the pharmacy, complaints that were treated were not ideal body weight (thin / bb down) and no appetite, experienced complaints for 1 week -> 20 years, 5.56% do self-medication for reasons that are cost-effective, many friends use and prove and 4.63% faster to obtain at low cost. A study with perception indicators, 75% said it was important to consider the recommendation of the pharmacist, 92.59% of patients wanted the drug to be used safely, 68.52% of respondents did self-medication because they had increased appetite and the body became fat to take the drug. as many as 81.48% of respondents said that the body's fattening drugs can increase appetite.