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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY BERNAS: Jurnal Pengabdian Kepada Masyarakat Jurnal Info Kesehatan
Rahajoe, Toeti
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Neuroscience Nursing Public Health Social Sciences Veterinary Other

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In vitro anticoagulant activity of crude fibrinolytic protease HSFT-2 as an alternative anticoagulant agent Afriansyah, Muhammad Ardi; Rahajoe, Toeti; Pranindira, Raksi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.5758

Abstract

Thrombotic disorders are one of the leading causes of death in cardiovascular patients and contribute to the incidence of non-infectious diseases. The search for new anticoagulant agents to overcome the limitations associated with existing anticoagulant therapies is increasing, especially those derived from natural and microbial sources. Staphylococcus hominis HSFT-2 is a Gram-positive coccus isolated from rusip Holothutia scabra commonly known as sand sea cucumber. Fibrinolytic protease HSFT-2 has been reported to be able to degrading fibrin, which has shown several characteristics of fibrinolytic and clot-lysis activities. This study aims to evaluate the anticoagulant activity of crude fibrinolytic protease HSFT-2 in vitro as potential alternative anticoagulant agents. The research design was completely randomized, with a sample size of 21 participants calculated using the Federer formula. The material used was crude protease derived from S. hominis cultured in Skim Milk Broth. Various tests, including blood routine tests, platelet aggregation tests, and blood smear evaluations, were conducted to assess its effectiveness as an anticoagulant. Whole blood samples were divided into three groups: a positive control, a negative control, and a group treated directly with 100 µL of crude fibrinolytic protease. The normality of the data was evaluated using the Kruskal-Wallis test, while differences between groups were analyzed with one-way ANOVA. The one-way ANOVA results from the blood routine tests indicated a significant difference between the control and the fibrinolytic protease HSFT-2 (p<0.05). The platelet aggregation percentage decreased from 75.85% in the control group to 21.75% with the addition of crude protease HSFT-2. Furthermore, the blood smear evaluations revealed no abnormalities in cell formation when HSFT-2 protease was used, compared to samples without the addition of EDTA anticoagulant. In conclusion, the fibrinolytic protease HSFT-2 exhibits anticoagulant activity; however, it does not yet meet the standards required for use as an anticoagulant in routine blood tests. Future work should focus on the purification and characterization of the protease to enhance its anticoagulant potential for laboratory diagnostic applications.
Edukasi Pemeriksaan Hematologi Rutin dan Pemeriksaan Golongan Darah pada Siswa SMP N 29 Semarang Saptaningtyas, Ragil; Rahajoe, Toeti; Lestari, Yunita; Maharani, Nadia
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 3 (2025)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v6i3.12642

Abstract

Pemeriksaan darah yang sering dilakukan adalah pemeriksaan hematologi rutin terutama pada remaja. Selain pemeriksaan haematologi rutin, pemeriksaan golongan darah juga sangat berguna untuk mengetahui golongan darah seseorang. Tujuan kegiatan pengabdian kepada masyarakat ini adalah untuk memberikan edukasi kepada siswa SMP N 29 Semarang tentang pemeriksaan hematologi rutin sekaligus melakukan pemeriksaan golongan darah ABO dan Rhesus. Kegiatan pengabdian kepada masyarakat telah dilakukan pada 8 Oktober 2024 di SMP N 29 Semarang yang berjumlah 34 siswa. Pemaparan meteri hematologi rutin dan golongan darah ABO disampaikan dengan Power point dan poster. Praktik emeriksaan golongan darah dilakukan secara langsung oleh para siswa yang bersedia dengan bantuan tim pengabdi. Perbedaan tingkat pemahaman para siswa diukur dengan hasil nilai pre test dan post test kemudian diuji dengan uji Wilcoxon. Kegiatan edukasi pemeriksaan hematologi rutin dan pemeriksaan golongan darah pada siswa SMP N 29 Semarang berjalan dengan lancar. Pemahaman para siswa meningkat secara signfikan setelah diberikan materi tentang pemeriksaan hematologi rutin dan golongan darah ABO. Sebanyak 73,5% bersedia melakukan pemeriksaan golongan darah dengan hasil yang terbanyak adalah golongan darah O Rhesus positif.
Single Nucleotide Variation in the Promoter Region of the APOA1 Gene as a Candidate Biomarker for Dyslipidemia Rahajoe, Toeti; Nugraheni, Kartika; Pinda Ayu Widiyani; Asihanto, Balqis Aureola Putri; Kartika, Aprilia Indra
JURNAL INFO KESEHATAN Vol 21 No 4 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss4.1222

Abstract

Dyslipidemia is a lipid profile abnormality that can escalate the risk of cardiovascular disease. The rate of cardiovascular events in Indonesia is very high. One of the causes of dyslipidemia is due to polymorphisms in genes associated with lipid metabolism. The APOA-1 gene encodes the APOA-1 protein which functions to regulate HDL protein synthesis. The objective of this study is to determine APOA1 gene polymorphisms in patients with dyslipidemia. Samples of healthy controls and dyslipidemia patients were used in this investigation. The lipid profiles of the patients and normal controls are determined at the onset of the study. Following DNA extraction, the APOA-1 gene was amplified and sequenced using the serum sample. MEGA X and BLAST were used to analyze the sequencing results. The study's findings demonstrated that the APOA-1 gene length in samples of dyslipidemic patients and normal controls was 433 bp. While the normal control samples have the same sequence as the database, the dyslipidemic patient samples have an APOA-1 gene polymorphism in the promoter region. APOA-1 gene polymorphism results in disturbances in lipid profiles, particularly HDL which is at risk of developing dyslipidemia. The APOA-1 gene has the potential to be developed as a biomarker for diagnosing dyslipidemia involving a larger number of samples.
Co-Authors Afriansyah, Muhammad Ardi Aprilia Indra Kartika, Aprilia Indra Asihanto, Balqis Aureola Putri Kartika Nugraheni Lestari, Yunita Nadia Maharani Pinda Ayu Widiyani Pranindira, Raksi Ragil Saptaningtyas, Ragil