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Penyuluhan dan Pemeriksaan Golongan Darah Sistem ABO - Rhesus Bagi Anak-Anak dan Pengelola Panti Asuhan Sonaf Maneka Kupang, Nusa Tenggara Timur Musa, Susan Hasanah; Mutmainnah, Siti; Ayanti, Bio Putri; Dewi, Okta Yosiana; Ernanto, Aditya Rahman; Sultistyaningtyas, Ayu Rahmawati; Ethica, Stalis Norma; Afriansyah, Muhammad Ardi
JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Vol 3 No 1 (2024): Januari
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jipmi.v3i1.294

Latar belakang: Golongan darah merupakan informasi yang penting bagi setiap individu, karena menentukan berbagai aspek medis seseorang yang meliputi antara lain keperluan transfusi darah, transplantasi organ dan kehamilan. Keberhasilan dalam berbagai aspek medis tersebut sangat ditentukan oleh kecocokan golongan darah. Hasil survei pendahuluan menunjukkan bahwa Anak-anak dan pengelola Panti Asuhan Katolik Sonaf Maneka Kupang banyak yang belum mengetahui manfaat mengetahui golongan darah dan belum memiliki informasi tentang golongan darahnya masing-masing. Tujuan: memberikan pengetahuan kepada anak-anak dan pengelola panti Asuhan Sonaf Manek di Kupang tentang pentingnya mengetahui golongan darah dan memberikan informasi tentang golongan darahnya masing-masing. Metode: Penyuluhan dilakukan oleh tim mahasiswa dan dosen Universitas Muhammadiyah dalam skema Pembangunan Kesehatan Masyarakat Desa (PKMD) dengan metode ceramah yang didukung penggunaan media poster. Evaluasi kegiatan penyuluhan dilakukan dengan kuesioner, sedangkan pemeriksaan golongan darah sistem ABO (A, B, AB dan O) dan Rhesus dilakukan secara langsung oleh mahasiswa Program Diploma Teknologi Laboratorium Medik. Hasil: Hasil analisis yang dilakukan menunjukkan bahwa kegiatan penyuluhan dengan media poster meningkatkan pemahaman peserta akan pentingnya pengetahuan tentang golongan darah. Kesimpulan: hasil pemeriksaan golongan darah menunjukkan bahwa anak-anak dan pengelola panti asuhan yang bergolongan darah A, B, AB, O berturut-turut 23, 43, 7, dan 27% serta seluruhnya memiliki golongan darah Rhesus Positif (Rh+). Kata kunci: media poster, panti asuhan, pemeriksaan golongan darah, pembangunan kesehatan masyarakat desa _______________________________________________________________________________ Abstract Background: Blood type is important information for every individual, as it determines various aspects of a person's medical such as the need for blood transfusions, organ transplants, and pregnancy. The compatibility of blood types largely determines success in various medical aspects. The preliminary survey results show that many children and tenants at the Sonaf Maneka Catholic Orphanage in Kupang did not know the benefits of knowing their blood type and did not yet have information about their respective blood groups. Objective: to provide knowledge to the children of the Sonaf Maneka Orphanage at Kupang about the importance of knowing their blood type and providing information about their respective blood groups. Method: Counseling was carried out by a team of students and lecturers of Muhammadiyah University in the Village Community Health Development (PKMD) scheme with the lecture method supported by media posters. Evaluation of activities carried out by questionnaires, ABO, and Rhesus system blood group examinations were carried out directly by students of the Diploma Program of Medical Laboratory Technology. Result: Results of the analysis showed that the outreach activities with posters increased participants' understanding of the importance of knowledge about blood groups. Conclusion: the results of the blood group examination showed that orphanage tenants with blood types A, B, AB, and O were 23, 43, 7, and 27% respectively and all were Rh-Positive (Rh+). Keywords: poster media, orphanage children, counseling, blood type test, village community health development

Pengaruh Sentrifugasi Spesimen Darah Anemia Terhadap Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Slide Handayani, Sri Puji; Sukeksi, Andri; Afriansyah, Muhammad Ardi
Jurnal Analis Kesehatan Vol. 11 No. 1 (2022): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v11i1.3196

Anemic patients often have low hemoglobin and red blood cell level, which on the slide method and ABO system blood type test is used to obtain low degree of agglutination. Addition of treatment of a centrifugation was used to obtain a stronger degree of agglutination. The purpose of this research was to determine the difference degree of agglutination between centrifuged and uncentrifuged blood specimens in anemic patients. Quasi experimental research with non-equivalent control group design was conducted in March 2022 at the Hematology Laboratory, Universitas Muhammadiyah Semarang. The research sample were 16 specimens of anemic patients for slide method and ABO system blood type test. All samples were checked for blood type testbefore being centrifuged, then re-checked after the centrifugation there only concentrated red blood cell deposit were taken with only a small amount of plasma. The data from the test were analyzed using the Kruskal Wallis method. Comparison anti-A, anti-B, and anti-AB between centrifuged and uncentrifuged blood specimens there was a significant difference (p value < 0,005 means p = 0,002). The result showed that there were effect by centrifugation for the degree of agglutination improved better in centrifuged if compared with uncentrifugedanemicpatients blood specimens on blood type test slide method.Â

Efek Homogenisasi Spesimen Darah Metode Inversi Terhadap Nilai Hematokrit Fadillah, Noor; Afriansyah, Muhammad Ardi; Sukeksi, Andri; Santosa, Budi
Jurnal Analis Kesehatan Vol. 12 No. 1 (2023): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i1.3714

Laboratory examination consists of three stages, namely pre-analytical, analytical, and post-analytic. The biggest error occurred in the pre-analytical stage, which was around 61%. Homogenization including the pre-analytical stage is the process of mixing blood and anticoagulants which are included in the pre-analytical stage. Homogenization has two ways namely manual and automatic. Inadequate homogenization can cause clots or rupture of erythrocytes/lysis and shrinkage of erythrocytes, leading to a falsely low hematocrit value. The study aimed to find out and analyze differences in hematocrit values by homogenizing the inversion method 2, 4, and 10 times. This type of research is observational analytic. The research was carried out in June - August 2021 at the Clinical Laboratory of Muhammadiyah University of Palangkaraya. The research sample consisted of 9 EDTA blood from 9 people according to the inclusion criteria. All samples were examined for hematocrit using a Hematology Analyzer Sysmex XP-300. The results showed that the average hematocrit value in 2 times inversion homogenization was 38.40%, 4 times homogenization was 38.78%, and 10 times homogenization was 39.14%. The conclusion of this study was that there was no significant effect of the inversion homogenization technique 2, 4, and 10 times on the hematocrit value (p=0.584 > 0.05).

Effect Of Donor Blood Storage Period on Hemoglobin Levels and Erythrocyte Cell Morphology Wulandari, Eka Wahyu Putri; Afriansyah, Muhammad Ardi; Sukeksi, Andri
Jurnal Kesehatan Manarang Vol 10 No 2 (2024): August 2024
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v10i2.929

Blood is a body fluid whose stability can be influenced by both internal and external factors such as storage period. Storing blood specimens for a long time can cause changes in the physiological condition of blood cells such as color, size, shape and cell formation. This study aims to determine the effect of the storage period for donor blood specimens at UTD RSUP dr. Kariadi Semarang on hemoglobin levels and erythrocyte morphology. The research design is an experiment using a modified Time Series design with a pre and posttest control group design. The samples in this study were CPDA-1 (Citrate Phosphate Dextrose Adenine) donor blood specimens at the Blood Transfusion Unit of RSUP dr. Kariadi Semarang. Observation of erythrocyte cell morphology using blood smear preparations (SHD). Primary data was taken from the results of measurements of hemoglobin levels and observations of erythrocyte cell morphology with blood specimens examined directly (day 0) and storage days 1, 2, and 3. Analysis of research data used the One-way Anova statistical test. The results of the One-Way Anova test showed that there was a significant difference in hemoglobin levels on the 3rd day of donor blood storage (p-value 0.000). The results of the evaluation of erythrocyte cell morphology showed that there were abnormalities in crenation morphology on the 3rd day of storage. Blood specimens should be processed immediately after they leave the body because the condition of the blood cells is still stable and optimal for use in laboratory tests.

Effect Of Donor Blood Storage Period on Hemoglobin Levels and Erythrocyte Cell Morphology Wulandari, Eka Wahyu Putri; Afriansyah, Muhammad Ardi; Sukeksi, Andri
Jurnal Kesehatan Manarang Vol 10 No 2 (2024): August 2024
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v10i2.929

Blood is a body fluid whose stability can be influenced by both internal and external factors such as storage period. Storing blood specimens for a long time can cause changes in the physiological condition of blood cells such as color, size, shape and cell formation. This study aims to determine the effect of the storage period for donor blood specimens at UTD RSUP dr. Kariadi Semarang on hemoglobin levels and erythrocyte morphology. The research design is an experiment using a modified Time Series design with a pre and posttest control group design. The samples in this study were CPDA-1 (Citrate Phosphate Dextrose Adenine) donor blood specimens at the Blood Transfusion Unit of RSUP dr. Kariadi Semarang. Observation of erythrocyte cell morphology using blood smear preparations (SHD). Primary data was taken from the results of measurements of hemoglobin levels and observations of erythrocyte cell morphology with blood specimens examined directly (day 0) and storage days 1, 2, and 3. Analysis of research data used the One-way Anova statistical test. The results of the One-Way Anova test showed that there was a significant difference in hemoglobin levels on the 3rd day of donor blood storage (p-value 0.000). The results of the evaluation of erythrocyte cell morphology showed that there were abnormalities in crenation morphology on the 3rd day of storage. Blood specimens should be processed immediately after they leave the body because the condition of the blood cells is still stable and optimal for use in laboratory tests.

Penyuluhan dan Pemeriksaan Tekanan Darah Pada Ibu PKK Perumahan Batursari Asri Demak Viandy, Amartya Desta; Zakiya, Roma Dhona Beauty; Cendani, Tinezia Rivan; Ethica, Stalis Norma; Aprianti, Nurannisa Fitria; Hikmah, Annisa Nurul; Afriansyah, Muhammad Ardi
JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Vol 2 No 3 (2023): Juli
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jipmi.v2i3.119

Latar belakang: Hipertensi dapat menjadi penyebab utama gagal jantung, stoke, dan gagal ginjal. Peningkatan tekanan darah sistolik menunjukan resiko yang lebih penting daripada peningkatan tekanan darah diastolik. Kemungkinan seseorang mengalami hipertensi akan semakin tinggi saat usia semakin bertambah. Tujuan: memberikan penjelasan mengenai pencegahan terjadinya Hipertensi dan Hipotensi. Pelaksanaan PKMD di perumahan Batursari Asri Blok B.87 RT.04 RW.05, Desa Batursari, Kecamatan Mranggen, Kabupaten Demak berdasarkan survey belum pernah dipakai oleh pelajar atau mahasiswa dalam melakukan kegiatan PKMD. Metode: Penyuluhan tentang tekanan darah diberikan secara langsung melalui poster. Evaluasi hasil kegiatan melalui pretest dan posttest. Pretest diberikan sebelum penyuluhan dan posttest serta pemeriksaan tekanan darah diberikan setelah penyuluhan. Hasil: Rerata nilai pretest peserta penyuluhan adalah 80 dengan nilai terendah adalah 60 yang diperoleh satu peserta dan tertinggi adalah 90 yang diperoleh dari enam peserta. Setelah diberikan penyuluhan peserta mendapatkan 10 pertanyaan yang sama seperti sebelumnya dan diperoleh nilai rerata posttest adalah 87,5 dengan nilai terendah 70 dan tertinggi adalah 100. Berdasarkan nilai pretest dan posttest menunjukkan terjadinya peningkatan pengetahuan pada peserta setelah diberikan penyuluhan. Kesimpulan: Peserta penyuluhan diperumaha Batursasri Asri mengalami peningkatan pengetahuan tentang resiko dan pencegahan hipertensi. Kata kunci: hipertensi, penyuluhan, tekanan darah ___________________________________________________________________________________ Abstract Background: Hypertension can be a major cause of heart failure, stoke, and kidney failure. Elevated systolic blood pressure indicates a more important risk than increased diastolic blood pressure. The possibility of someone experiencing hypertension will be higher as age increases. Objective: provide an explanation of the prevention of Hypertension and Hypotension. The implementation of PKMD in Batursari Asri housing RT.04 RW.05, Batursari Village, Mranggen District, Demak Regency based on surveys has never been used by students or students in carrying out PKMD activities. Method: Counseling on blood pressure is given directly through posters. Evaluation of activity results through pretest and posttest. Pretest is given before counseling and a posttest and blood pressure check is given after counseling. Result: The average pretest score of counseling participants was 80 with the lowest score being 60 obtained by one participant and the highest was 90 obtained from six participants. After being given counseling, participants received the same 10 questions as before and obtained the average posttest score was 87.5 with the lowest score of 70 and the highest was 100. Based on pretest and post-test scores, it shows an increase in knowledge in participants after being given counseling. Conclusion: Counseling participants in Batursasri Asri housing experienced increased knowledge about the risks and prevention of hypertension. Keywords: hypertension, counseling, blood pressure

PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10 Ainutajriani, Ainutajriani; Darmawati, Sri; Zilda, Dewi Seswita; Afriansyah, Muhammad Ardi; Saptaningtyas, Ragil; Ethica, Stalis Norma
BIOTROPIA Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1765

Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.

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