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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Journal of Tropical Diversity
Ariyani, Destia Fitri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Pendugaan Fekunditas Induk Betina Ikan Nilem (Osteochilus vittatus) berdasarkan Karakter Morfometrik Ramadhan, Surya Dewa; Widodo, Maheno Sri; Ariyani, Destia Fitri; Pratama, Rakhmad Ndaru; Dailami, Muhammad
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 7 No 2 (2024): ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua
Publisher : Cenderawasih University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/acr.v7i2.4389

Abstract

Ikan nilem merupakan ikan air tawar asli Indonesia yang persebaranya hanya di daerah Jawa Barat. Induk betina ikan nilem memiliki jumlah telur yang bervariasi sehingga perlu dilakukan seleksi. Seleksi yang dapat dilakukan pada induk betina ikan nilem yaitu dengan cara melihat karakter morfometrik. Sampai saat ini penelitian tentang ikan nilem khususnya yang berkaitan dengan reproduksi juga masih sangat jarang dilakukan. Tujuan dilaksanakanya penelitian ini adalah untuk menganalisis hubungan dan mengetahui karakter morfometrik yang paling berhubungan terhadap fekunditas. Metode yang digunakan dalam penelitian iniyaitu deskripstif komparatif dengan pendekatan kuantitatif. Parameter yang diamati meliputi panjang standar, tinggi badan, lingkar tubuh, dan bobot tubuh. Kelompok sampel ikan uji diukur berdasarkan panjang standar yang telah ditentukan, yaitu 06,1-09 cm, 09,1-12 cm, 12,1-15 cm, 15,1- 18 cm, 18,1-21 cm. Hasil penelitian menunjukkan bahwa morfometrik berpengaruh terhadap fekunditas. Karakter morfometrik terhadap fekunditas diperoleh hasil tertinggi pada lingkar tubuh dengan fekunditas sebesar 99%terhadap fekunditas yang dihasilkan pada induk betina ikan nilem. Faktor yang dapat mempengaruhi jumlah fekunditas diantaranya yaitu umur ikan, lingkungan, makanan, dan diameter ikan. Diameter ikan sangat berpengaruh terhadap jumlah fekunditas yang di hasilkan, semakin besar diameter telur dalam suatu gonad maka akan semakin sedikit jumlah telur yang dihasilkan.
Identifikasi Genetik Ikan Nilem dengan Pendekatan DNA Barcoding Dailami, Muhammad; Yuniarti, Ating; Widodo, Maheno Sri; Pratama, Rakhmad Ndaru; Ariyani, Destia Fitri; Siahaan, Marthin Daniel Theo Gratia; Maulana, Antajala Rian; Ramadhan, Surya Dewa; Putri, Radwa Fathin H; Nurcahyani, Shinta Eka; Milansari, Sindi; Mayor, Corazon Ell Merilia Papuani
Journal of Tropical Diversity Vol 1 No 1 (2025): Februari
Publisher : Penerbit Brainy Bee

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64283/jotdiverse.2025.1(1):3

Abstract

Nilem fish (Osteochilus vittatus) is a species of freshwater fish from the Cyprinidae family which has ecological and economic importance in Indonesia, with its natural habitat in rivers, swamps and stagnant waters. Accurate species identification is very important in biological research and fisheries resource management, but morphological identification methods have several limitations. DNA barcoding is a revolutionary molecular technique that uses short DNA sequences to identify and differentiate species with high accuracy. The use of DNA barcoding in identifying freshwater fish in Indonesia has shown significant development, including in a comprehensive study documenting 1,172 native freshwater fish species. The research "Genetic Identification of Nilem Fish using the DNA Barcoding Approach" aims to characterize the genetics of nilem fish, compare the genetic profiles of various populations, and evaluate the effectiveness of DNA barcoding in distinguishing nilem fish from other Osteochilus species, with the hope of contributing to fish conservation and sustainable management efforts nilem in Indonesia. The DNA sequencing results obtained a sequence with a length of 619 base pairs with the composition of Nucleotides A (27.2%), G (17.6%), C (26.4%), T (28.8%). The BLAST results with the NCBI database showed a similarity of 99.84% with the O. vittatus sequence and 99.83% with O. hasseltii. Both species are synonyms with the official name O. vittatus. Phylogenetic analysis is also in line with the BLAST results which show that the sample sequence is in the same clade as the O. vittatus and O. hasseltii sequences. Identification with molecular markers of the COI gene was successfully used in determining the species of the Nilem fish (O. vittatus).
DNA Barcoding of Snapper Fish (Lutjanus spp.) from Kaimana and Fakfak, West Papua Ariyani, Destia Fitri; Kusuma, Wahyu Endra; Pranata, Bayu; Manangkalangi, Emmanuel; Jeni, Jeni; Toha, Abdul Hamid A.; Dailami, Muhammad
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 31, No 1 (2026): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.31.1.26-36

Abstract

Kaimana and Fakfak are two regions in West Papua which have high biodiversity. The total fish production in Kaimana and Fakfak are 10,039 tons.y-1 and 17,806 tons.y-1, respectively. The snapper fish (Lutjanus spp.) is one of the important economic commodities in Kaimana and Fakfak regions. There has been a decrease in the number of exports of snapper fish in 2018 to 2019 from 4,742 tons to 4,290 tons due to overfishing and environmental pollution. This study employed DNA barcoding technology to identify the species of snapper fish collected from Kaimana and Fakfak. The DNA isolation was conducted by using genomic DNA mini kit (tissue) and the amplification of COI gene with Go Taq green master mix. Agarose gel electrophoresis was used to visualize the PCR product.  A total of 16 sequences with length 654 base pairs of COI gene were identified as five species of Lutjanidae, which were Lutjanus decussatus, L. gibbus, L. quinquelineatus, L. malabaricus, and L. johnii. Homology analysis with BLAST NCBI and BOLD System showed that all samples have similarity of 99.08-100% and query cover of 93-100%. Relationship analysis using phylogenetic tree and genetic distances showed results of intraspecific close relatives (0.001-0.016) and interspecific distant relatives (>0.1000). The phylogenetic tree illustrated that all species of Lutjanidae are separated into monophyletic clades. DNA barcoding technology successfully identified the snapper fish collected from Kaimana and Fakfak. 
Co-Authors Abdul Hamid A. Toha Ating Yuniarti Bayu Pranata, Bayu Emmanuel Manangkalangi Jeni, Jeni Kusuma, Wahyu Endra Maheno Sri Widodo, Maheno Sri Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Muhammad Dailami, Muhammad Nurcahyani, Shinta Eka Pratama, Rakhmad Ndaru Putri, Radwa Fathin H Ramadhan, Surya Dewa Siahaan, Marthin Daniel Theo Gratia