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All Journal Jurnal Biologi Tropis Journal of Tropical Diversity
Milansari, Sindi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Genetic Diversity of Red Snapper (Lutjanus erythropterus) in Kaimana Waters Milansari, Sindi; Toha, Abdul Hamid A.; Pranata, Bayu; Dailami, Muhammad; Manangkalangi, Emmanuel; Jeni, Jeni
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8840

Abstract

Genetic diversity is one level of biodiversity that is very importan for the resilience of biota in the face of environmental pressures such as cilmater change. However, information on the genetic diversity of red snapper in Kaimana water is still very limited. This study determines the genetic diversity of red snapper (Lutjanus erythropterus) in Kaimana waters, West Papua, to support conservation and sustainable management. The methods used include PCR, DNA sequencing, and phylogenetic analysis using MEGA11, with samples taken from the Kaimana fish market. The results of this study show differences in the percentage of adenine and guanine in sample B81.01, with an average codon composition of 218 codons, where UCU(G) Serine has the highest percentage of 13.8% and RSCU 2.38%, and 20 types of amino acids were detected. Lutjanus erythropterus has 3 haplotypes, with 2 universal haplotypes and 1 specific. In Kaimana waters, the haplotype diversity (hd) value is 0.50000 and the nucleotide diversity (π) is 0.00091, which is influenced by the large population size and high migration ability.
Identifikasi Genetik Ikan Nilem dengan Pendekatan DNA Barcoding Dailami, Muhammad; Yuniarti, Ating; Widodo, Maheno Sri; Pratama, Rakhmad Ndaru; Ariyani, Destia Fitri; Siahaan, Marthin Daniel Theo Gratia; Maulana, Antajala Rian; Ramadhan, Surya Dewa; Putri, Radwa Fathin H; Nurcahyani, Shinta Eka; Milansari, Sindi; Mayor, Corazon Ell Merilia Papuani
Journal of Tropical Diversity Vol 1 No 1 (2025): Februari
Publisher : Penerbit Brainy Bee

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64283/jotdiverse.2025.1(1):3

Abstract

Nilem fish (Osteochilus vittatus) is a species of freshwater fish from the Cyprinidae family which has ecological and economic importance in Indonesia, with its natural habitat in rivers, swamps and stagnant waters. Accurate species identification is very important in biological research and fisheries resource management, but morphological identification methods have several limitations. DNA barcoding is a revolutionary molecular technique that uses short DNA sequences to identify and differentiate species with high accuracy. The use of DNA barcoding in identifying freshwater fish in Indonesia has shown significant development, including in a comprehensive study documenting 1,172 native freshwater fish species. The research "Genetic Identification of Nilem Fish using the DNA Barcoding Approach" aims to characterize the genetics of nilem fish, compare the genetic profiles of various populations, and evaluate the effectiveness of DNA barcoding in distinguishing nilem fish from other Osteochilus species, with the hope of contributing to fish conservation and sustainable management efforts nilem in Indonesia. The DNA sequencing results obtained a sequence with a length of 619 base pairs with the composition of Nucleotides A (27.2%), G (17.6%), C (26.4%), T (28.8%). The BLAST results with the NCBI database showed a similarity of 99.84% with the O. vittatus sequence and 99.83% with O. hasseltii. Both species are synonyms with the official name O. vittatus. Phylogenetic analysis is also in line with the BLAST results which show that the sample sequence is in the same clade as the O. vittatus and O. hasseltii sequences. Identification with molecular markers of the COI gene was successfully used in determining the species of the Nilem fish (O. vittatus).
Co-Authors Abdul Hamid A. Toha Ariyani, Destia Fitri Ating Yuniarti Bayu Pranata, Bayu Emmanuel Manangkalangi Jeni, Jeni Maheno Sri Widodo, Maheno Sri Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Muhammad Dailami, Muhammad Nurcahyani, Shinta Eka Pratama, Rakhmad Ndaru Putri, Radwa Fathin H Ramadhan, Surya Dewa Siahaan, Marthin Daniel Theo Gratia