Article Per Year (5 Year)
p-Index From 2021 - 2026
0.751
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal JURNAL SELULOSA 3BIO: Journal of Biological Science, Technology and Management Current Research on Biosciences and Biotechnology
Wardono Niloperbowo
School Of Life Science And Technology, Institut Teknologi Bandung, Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Integration Stability of sHBsAg-Multi Expression Cassettes in Pichia pastoris GS115 during Methanol Induction Patricia Gita Naully; Neni Nurainy; Elvi Restiawaty; Dessy Natalia; Debbie Soefie Retnoningrum; Wardono Niloperbowo; Ernawati Arifin Giri-Rachman
HAYATI Journal of Biosciences Vol. 27 No. 4 (2020): October 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.4.283

Abstract

Hepatitis B is the major health problem worldwide including in Indonesia. Vaccination is the best prevention strategy for the disease. For the purpose of vaccine development and to decrease drug import, production of Hepatitis B Virus (HBV) small surface antigen (sHBsAg) from Indonesian HBV subtype is needed. The recombinant protein production can be conducted by integrating multi expression cassettes of sHBsAg gene in Pichia pastoris chromosome using gene replacement method. Such integration method turns out to allow loss of foreign gene from chromosome by excisional recombination-mediated looping out. This research was aimed to determine integration stability of four copies of sHBsAg expression cassette in P. pastoris GS115 chromosome inducted with 2% methanol in FM22 medium. The methanol induction was conducted twice at 63-h and 75-h. Integration stability determination was conducted qualitatively using PCR and quantitatively using qPCR absolute quantification. A band of 208 bp with similar intensity was observed after amplification of genomic DNA. All samples generated the same Ct value of around 22 with four copies of sHBsAg gene per genome. The result from this experiment shows that integration of four copies of sHBsAg expression cassette in P. pastoris GS115 chromosome is stable during methanol induction.
PRODUKSI LAKASE DAN POTENSI APLIKASINYA DALAM PROSES PEMUTIHAN PULP (LACASE PRODUCTION AND ITS POTENTIAL IN PULP BLEACHING PROCESS) Hendro Risdianto; Sri H. Suhardi; Wardono Niloperbowo; Tjandra Setiadi
JURNAL SELULOSA Vol 43, No 01 (2008): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5464.342 KB) | DOI: 10.25269/jsel.v43i01.162

Abstract

This study was focused on production and utilisation of laccase in pulp bleaching process. Laccase was produced by white-rot fungi of Marasmius sp which was immobilised in luffa sponge in a modified immersion bioreactor. The Experiment was conducted to study the effect of variation of immersion periods in the production of laccase. The immersion periods applied in this study were 15 minutes, 12 hours and 24 hour respectively. Lacase was then utilised in pre-treatment of pulp bleaching of Accacia mangium unbleached-pulp. The bleaching process were conducted with and without addition mediator of 2,2'-azinobis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) which has ability to enhance the delignification process.The results showed that immersion period of 12 hours exhibited the highest maximum laccase activity. Maximum level of laccase attained from culture with 12 hours immersion period was 457.6 U/L. When the immersion period be double ( 24 hours), the level of laccase, however, becoming lower i.e. 281.9 U/L. A 15 minutes immersion period has resulted in a level of laccase of 384.4 U/L. Utilisation of crude enzymes by addition of ABTS mediator with enzyme exposure time 6 hours at temperature of 45oC has increased brightness for 2.8 points. Pretreatment in similar conditions with no ABTS has added showed an increase in brightness for 0.7 point. It demonstrated that the addition  of ABTS has improved biobleaching process. The pulp exposed to crude enzymes for 6 days at room temperature has increased the brightness of pulp to 5.3 points. These results indicate that laccase has a potential ability  in pulp bleaching process.Keywords: a modified temporary immersion bioreactor, bleaching, brightness\, crude enzyme, laccase, luffa, Marasmius sp., white-rot fungi.INTISARIPenelitian ini ditujukan untuk memproduksi lakase dan menggunakannya dalam proses pemutihan pulp. Produksi lakase dilakukan dengan menggunakan jamur pelapuk putih Marasmius sp. yang diimobilisasi pada bulustru. Bioreaktor yang digunakan adalah bioreaktor imersi berkala termodifikasi. Penelitian produksi lakase dilakukan dengan variasi waktu imersi 15 menit, 12 jam dan 24 jam. Produk berupa ekstrak kasar lakase digunakan sebagai perlakuan awal proses pemutihan pulp Acasia mangium. Pemutihan pulp dilaksanakan dengan dan tanpa mediator 2,2'-azinobis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) yang berfungsi untuk meningkatkan proses delignifikasi.Hasil penelitian menunjukkan bahwa waktu immersi 12 jam menghasilkan lakase dengan aktivitas paling tinggi yaitu mencapai 457,6 U/L, lebih tinggi dibandingkan waktu immersi 15 menit (348,4 U/L) dan waktu imersi 24 jam (281,9 U/L). Pemutihan pulp menggunakan ekstrak kasar lakase dengan bantuan ABTS selama 6 jam dan suhu 45oC  dapat meningkatkan derajat putih sebesar 2,8 poin. Sedangkan pada kondisi yang sama namun tanpa penambahan ABTS hanya dapat meningkatkan derajat putih 0,7 poin. Hal ini menunjukkan bahwa penggunaan ABTS dapat meningkatkan proses pemutihan. Sementara penggunaan ekstrak kasar lakase selama enam hari dapat meningkatkan derajat putih sebesar 5,3 poin. Dengan demikian, lakase memiliki potensi untuk peningkatan derajat putih proses pemutihan pulp yang diharapkan dapat mengurangi kebutuhan bahan kimia pemutih dan mengurangi limbah berbahaya proses pemutihan.Kata kunci: bioreaktor imersi berkala termodifikasi, pemutihan, derajat putih, ekstrak kasar, lakase, bulustru, Marasmus sp., jamur pelapuk putih.
Kajian Histologis Infeksi LD50 SLNPV terhadap Kerusakan Membran Peritrofik Larva Spodoptera litura Fabricius Yayan Sanjaya; Nanin Diah; Wardono Niloperbowo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 3 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.3.273

Abstract

The effect of SlNPV infection on  peritrophic membrane  of Spodoptera litura has been carried out. The damage of histological structure caused by SlNPV (0, 315, 390, 465, 540 and 615 PIB/ml) was investigated after 0, 12, 24 ,72 and 96 hours post infection. The histological material was prepared by using parafin method after fixation with Bouin Solution, then sliced into 7 mm and stained with Hematoxilin-Eosin.   The descriptive obsevation on structural intact of peritrophic membrane histology caused by SlNPV infection shows a tendency to decrease, while in control, there was no damage at all.  The longer the exposition of virion in the midgut lumen the more damage on peritrophic membrane occurred. The severest damage occurred 96 hour after infection. The result prove that haNPV virion can destroy histological structure of midgut
Anti-HBsAg IgY polyclonal antibodies potential as capture antibody for HBsAg detection kit development A'yun, Ramadhani Qurrota; Hakim, Meutia Diva; Giri-Rachman , Ernawati Arifin; Tan, Marselina; Niloperbowo, Wardono
Current Research on Biosciences and Biotechnology Vol. 5 No. 2 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.5.2/UBVBMKPZ

Abstract

Hepatitis B, affecting about 296 million globally, is a significant concern, with Indonesia ranking second in Southeast Asia for case numbers. The disease's latent initial phase, devoid of early symptoms upon hepatitis B virus (HBV) infection, highlights the demand for precise diagnostics. This research aimed to develop anti-HBsAg polyclonal antibodies (pAb) for application as capture agents within a sandwich enzyme-linked immunosorbent assay (ELISA). Chicken egg-derived IgY antibodies have advantages over mammalian ones due to simpler extraction and higher yield. In a study involving 21-week-old chickens, four intramuscular injections of 500 µg HBsAg antigen in Freund's adjuvant were administered at two-week intervals. Eggs as IgY source were collected daily and then IgY was isolated from eggyolk using polyethylene glycol (PEG) precipitation. The Bradford method was used to measure the total protein concentration, while the existence of IgY and pAb specific IgY against Anti-HBsAg was verified through SDS-PAGE and sandwich ELISA using HRP as a reporter, respectively. The resulting SDS-PAGE showed two distinct IgY bands: a 68 kDa heavy chain and a 23 kDa light chain. Using these anti-HBsAg IgY antibodies as capturing agents, the slightly elevation of IgY pAb against HBsAg level has been identified within the second week following the initial immunization. Subsequently, from the third to the eighth week, antibody levels escalated significantly, ranging from 2 to 13-fold higher than those observed in the second week. These findings suggest the potential use of IgY pAb as effective capture antibodies in sandwich ELISA for HBsAg antigen detection.
A Brief Review of the Global and Indonesian Diagnostic Development for Sexual Transmitted Diseases Giri-Rachman, Ernawati Arifin; Laurelia, Jessica; Marselina Irasonia, Tan; Wardono , Niloperbowo; Anindyajati
Current Research on Biosciences and Biotechnology Vol. 6 No. 1 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.6.1/RIQI8H4A

Abstract

Sexually transmitted diseases (STDs) are diseases with a high prevalence rate. The World Health Organization (WHO) estimates that more than 1 million STDs are transmitted every day, those diseases are chlamydia, syphillis, trichomoniasis, ghonorroea, and virus caused diseases (hepatitis B virus, immunodeficiency virus, human papillomavirus, and herpes simplex virus). Early and accurate examination and detection are important to help with the healing and control of these diseases. One of the examination methods that could be used is using the laboratory diagnostic methods which contribute to 40% to 60% of the process of diagnosing a disease. Thus, early detection not only helps control the spread of STDs but also facilitates the healing process. However, in Indonesia there are obstacles in the examination of diseases due to several factors, such as inadequate surveillance system and limited examination facilities, so that most people are undiagnosed. Therefore, this review will discuss in more depth the development of diagnostics for sexually transmitted diseases in Indonesia and globally. Global development in diagnostics is very broad and diverse, in which many diagnostic techniques has already been established. The development of diagnostic techniques has progressed rapidly from simplex assays to multiplex and also computerized assays. Diagnostic techniques in Indonesia has also developed and some of the local kits have already been in the market showing that Indonesia is already moving towards production of local diagnostics.
The Influence of Polyethylene Glycol Precipitation Methods on Yield and Purity of White Radish Peroxidase Faizah, Nur Al; Giri-Rachman, Ernawati Arifin; Niloperbowo, Wardono
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.5

Abstract

Proteins are widely used in various industries as highly valued biotechnology products. One example is horseradish peroxidase isolated from horseradish (Armoracia rusticana) that used as enzyme label in immunochemistry. However, the cultivation of horseradish is limited to subtropical countries, making the dependency on horseradish peroxidase unsustainable for tropical countries. Numerous studies have explored alternative peroxidases, and white radish peroxidase isolated from Raphanus sativus L. has emerged as a promising candidate. In this study, white radish peroxidase is isolated using the polyethylene glycol (PEG) precipitation method which is widely used as a simple and cost-effective method. This study aims to evaluate the effectiveness of the one-step and two-step PEG precipitation method. The one-step PEG precipitation method used in this study was done by mixing the white radish juice with PEG 6000 30% (w/v), while the two-step method was done by mixing it with PEG 400 20% (w/v) and PEG 6000 30% (w/v) consecutively. This study compares the yield and recovery levels of total protein and white radish peroxidase, as well as the enzymatic specific activity of white radish peroxidase isolated both by the one-step PEG precipitation and the two-step PEG precipitation. The results indicate that both extraction methods yield the same level of white radish peroxidase. However, they differ in terms of purity. The two-step extraction method results in white radish peroxidase with higher purity, as evidenced by its specific activity towards the chromogen ABTS in the presence of H2O2.
Bioconversion of inorganic selenium to organic selenium in the black soldier fly (BSF) larvae Sihombing, Rolina Anna Erica; Niloperbowo, Wardono; Nugrahapraja, Husna
Current Research on Biosciences and Biotechnology Vol. 7 No. 1 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.7.1/JAFFPU8L

Abstract

Selenium is one of the essential micronutrients needed to fulfil livestock nutrition, which can be found in inorganic and organic forms. Black Soldier Fly (BSF) larvae can potentially be used as a natural converter from inorganic selenium to organic selenium. However, the capacity and response of BSF larvae to convert selenium are still unknown. This study aims to determine the effect of inorganic selenium administration on BSF larvae. The research method was the determination of selenium concentration by UV-Vis spectrophotometry method based on the variation in the age of inorganic selenium administration in BSF larvae to the growth of BSF larvae, and the accumulation of selenium in BSF larvae, with variations in age of 0, 4, 8, and 12 days of age given sodium selenite (Na2SeO3) and control without administration of sodium selenite from the beginning to the end of rearing. There was no significant difference (p > 0.05) in the growth performance of BSF larvae and the accumulation of selenium in BSF larvae in the age variation experiment of BSF larvae when given sodium selenite. Based on the acquisition of larval mass, the growth rate of BSF larvae in the control treatment, with sodium selenite at 1 mg/kg, was 0.129, 0.093, 0.037, 0.156, and 0.128 mg/day at 0, 4, 8, and 12 days, respectively. These results indicate that inorganic selenium administration to BSF larvae can occur during the rearing period. In the experiment of variation in the concentration of inorganic selenium given to BSF larvae, the growth rate of BSF larvae was significantly higher (p < 0.05) when given 1000 mg/kg of sodium selenite, which was 0.467 mg/day.
Co-Authors . Anindyajati A'yun, Ramadhani Qurrota DEBBIE SOEFIE RETNONINGRUM Dessy Natalia Elvi Restiawaty ERNAWATI ARIFIN GIRI-RACHMAN Faizah, Nur Al Hakim, Meutia Diva Hendro Risdianto Laurelia, Jessica Marselina Irasonia, Tan Marselina Tan Nanin Diah Neni Nurainy Nugrahapraja, Husna Patricia Gita Naully Setiadi, Tjandra Sihombing, Rolina Anna Erica Sri H. Suhardi YAYAN SANJAYA