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Calyptranti, Rosellynia
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Medicine & Pharmacology Public Health

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Mangiferin Content in N-Hexane and Diethyl Ether Fractions of Mango (Mangifera indica L.) Leaves Var. Gedong Gincu : Luviriani, Eva; Calyptranti, Rosellynia; Jehan, Nabilah Nauli
Sebatik Vol. 28 No. 2 (2024): December 2024
Publisher : STMIK Widya Cipta Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46984/sebatik.v28i2.2513

Abstract

Mango (Mangifera indica L.) is a tropical fruit plant with high economic value and potential as a source of phytopharmaceuticals. One notable mango variety is Gedong Gincu, originating from Majalengka Regency, West Java, which contains active compounds such as mangiferin, flavonoids, saponins, and tannins with various therapeutic effects. This study aims to compare the mangiferin content in the n-hexane and diethyl ether fractions of Gedong Gincu mango leaf extract using the Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) method. Mango leaves were extracted with ethanol, followed by fractionation using n-hexane and diethyl ether. The resulting fractions were then analyzed for mangiferin content using RP-HPLC. The weights of the n-hexane and diethyl ether fractions were 13.418 g and 8.21 g, respectively. Phytochemical screening revealed the presence of flavonoids, alkaloids, phenols, tannins, and saponins. RP-HPLC analysis showed differences in mangiferin content between the two fractions, with retention times (RT) of 1.430 minutes for the n-hexane fraction and 1.473 minutes for the diethyl ether fraction, indicating variations in solubility and interaction with the mobile phase. The RP-HPLC quantification revealed that the n-hexane fraction contained 339.5 mg/L of mangiferin, while the diethyl ether fraction contained 547.3 mg/L. This study provides valuable insights into the comparison of extraction solvents for mangiferin, which can be further utilized in the development of herbal formulations.
Kualitas mikrobiologi basis pelembab dari lemak daging Sapi metode low temperature dan bouble boiling berdasarkan angka lempeng total (ALT) Kurniawati, Triani; Luviriani, Eva; Rahasasti, Indri Dwi; Calyptranti, Rosellynia; Shafly, Alman
Borobudur Pharmacy Review Vol 5 No 1 (2025): January- June
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/bphr.v5i1.13560

Abstract

Dry skin is a problem that bothers many people. One of the causes of dry skin is skin dehydration, which can be overcome by using moisturizing cosmetics. Vaseline is a moisturizer commonly used by the public because it does not cause allergies and has good moisturizing capabilities. However, it is derived from non-renewable petroleum, necessitating alternatives. Animal fats can be used as cosmetic moisturizers and are considered safer than chemical substances like zinc oxide. According to BPOM, cosmetic products must pass safety tests such as microbial contamination, heavy metal contamination, and chemical contamination tests. Beef fat contains water and fatty acids that can create a suitable medium for microbial growth. However, temperature and water content affect microbial growth, necessitating further research on the microbiological quality of beef fat using low temperature and double boiling methods. This study aims to determine the microbiological quality of beef fat moisturizer bases using low temperature and double boiling methods through Total Plate Count (TPC) testing. This research is a pre-experimental study with a one-shot case study design. The results show that the TPC values of beef fat using low temperature and double boiling methods are each 1 x 10^4 CFU/g. This indicates that the TPC values of beef fat using low temperature and double boiling methods do not meet BPOM criteria, which is < 500 CFU/g. Keywords: Moisturizer; Beef Tallow; Low Temperature; Double Boiling; Total Plate Count.
Co-Authors Indri Dwi Rahasasti Jehan, Nabilah Nauli Luviriani, Eva Shafly, Alman Triani Kurniawati, Triani